Abstract:
:Pfs expression is required for several metabolic pathways and limits the production of autoinducer-2, a molecule proposed to play a central role in interspecies quorum sensing. The present study reveals physiological conditions and promoter DNA elements that regulate Escherichia coli pfs transcription. Pfs transcription is shown to rely on both sigma 70 and sigma 38 (rpoS), and the latter is subject to induction that increases pfs expression. Transcription is maximal as the cells approach stationary phase, and this level can be increased by salt stress through induction of sigma 38-dependent expression. The pfs promoter is shown to contain both positive and negative elements, which can be used by both forms of RNA polymerase. The negative element is contained within the overlapping dgt promoter, which is involved in purine metabolism. Consideration of the physiological roles of sigma 38 and dgt leads to a model for how autoinducer production is controlled under changing physiological conditions.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Kim Y,Lew CM,Gralla JDdoi
10.1128/JB.00868-06subject
Has Abstractpub_date
2006-11-01 00:00:00pages
7457-63issue
21eissn
0021-9193issn
1098-5530pii
JB.00868-06journal_volume
188pub_type
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