Effect of deoxyribonucleic acid replication inhibitors on bacterial recombination.

Abstract:

:Two inhibitors of replicative deoxyribonucleic acid (DNA) synthesis, nalidixic acid (NAL) and 6-(p-hydroxyphenylazo)-uracil (HPUra), showed different effects on genetic recombination and DNA repair in Bacillus subtilis. Previous work (Pedrini et al., 1972) showed that NAL does not interfere with the transformation process of B. subtilis. The results reported in this work demonstrated that the drug was also without effect on the transfection by SPP1 or SPO-1 phage DNA (a process that requires a recombination event). The drug was also ineffective on the host cell reactivation of ultraviolet-irradiated SPP1 phage, as well as on transfection with ultraviolet-irradiated DNA of the same phage. HPUra instead markedly reduced the transformation process, as well as transfection, by SPO-1 DNA, but it did not affect the host cell reactivation of SPO-1 phage. In conclusion, whereas the NAL target seems to be specific for replicative DNA synthesis, the HPUra target (i.e., the DNA polymerase III of B. subtilis) seems to be involved also in recombination, but not in the excision repair process. The mutations conferring NAL and HPUra resistance used in this work were mapped by PBS-1 transduction.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Canosi U,Siccardi AG,Falaschi A,Mazza G

doi

10.1128/JB.126.1.108-121.1976

subject

Has Abstract

pub_date

1976-04-01 00:00:00

pages

108-21

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

126

pub_type

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