A complex network regulates expression of eps and other virulence genes of Pseudomonas solanacearum.

Abstract:

:We have discovered an unusual and complex regulatory network used by the phytopathogen Pseudomonas solanacearum to control transcription of eps, which encodes for production of its primary virulence factor, the exopolysaccharide EPS I. The major modules of this network were shown to be three separate signal transduction systems: PhcA, a LysR-type transcriptional regulator, an dual two-component regulatory systems, VsrA/VsrD and VsrB/VsrC. Using lacZ fusions and RNA analysis, we found that both PhcA and VsrA/VsrD control transcription of another network component, xpsR, which in turn acts in conjunction with vsrB/vsrC to increase transcription of the eps promoter by > 25-fold. Moreover, gel shift DNA binding assays showed that PhcA specifically binds to the xpsR promoter region. Thus, the unique XpsR protein interconnects the three signal transduction systems, forming a network for convergent control of EPS I in simultaneous response to multiple environmental inputs. In addition, we demonstrate that each individual signaling system of the network also acts independently to divergently regulate other unique sets of virulence factors. The purpose of this complex network may be to allow this phytopathogen to both coordinately or independently regulate diverse virulence factors in order to cope with the dynamic situations and conditions encountered during interactions with plants.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Huang J,Carney BF,Denny TP,Weissinger AK,Schell MA

doi

10.1128/jb.177.5.1259-1267.1995

subject

Has Abstract

pub_date

1995-03-01 00:00:00

pages

1259-67

issue

5

eissn

0021-9193

issn

1098-5530

journal_volume

177

pub_type

杂志文章
  • Biochemical studies of bacterial sporulation and germination. XII. A sulfonic acid as a major sulfur compound of Bacillus subtilis spores.

    abstract::A sulfonic acid found to be a major constituent of spores of Bacillus subtilis was provisionally identified as 3-l-sulfolactic acid. This compound was completely absent from vegetative cells during growth, but large amounts accumulated in sporulating cells just before the development of refractile spores. Essentially ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.1.62-68.1969

    authors: Bonsen PP,Spudich JA,Nelson DL,Kornberg A

    更新日期:1969-04-01 00:00:00

  • Mutations that affect control of the methylesterase activity of CheB, a component of the chemotaxis adaptation system in Escherichia coli.

    abstract::Sensory adaptation by the chemotaxis system of Escherichia coli requires adjustments of the extent of methyl esterification of the chemotaxis receptor proteins. One mechanism utilized by E. coli to make such adjustments is to control the activity of CheB, the enzyme responsible for removing receptor methyl ester group...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.6.3388-3399.1990

    authors: Stewart RC,Roth AF,Dahlquist FW

    更新日期:1990-06-01 00:00:00

  • A Gly145Ser substitution in the transcriptional activator PrfA causes constitutive overexpression of virulence factors in Listeria monocytogenes.

    abstract::Virulence genes in Listeria monocytogenes are coordinately expressed under the control of the transcriptional activator PrfA, encoded by prfA, a member of the cyclic AMP (cAMP) receptor protein (CRP)/FNR family of bacterial regulators. Strain P14-A is a spontaneous mutant of L. monocytogenes serovar 4b which produces ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.5.1533-1540.1997

    authors: Ripio MT,Domínguez-Bernal G,Lara M,Suárez M,Vazquez-Boland JA

    更新日期:1997-03-01 00:00:00

  • An Extended Cyclic Di-GMP Network in the Predatory Bacterium Bdellovibrio bacteriovorus.

    abstract:UNLABELLED:Over the course of the last 3 decades the role of the second messenger cyclic di-GMP (c-di-GMP) as a master regulator of bacterial physiology was determined. Although the control over c-di-GMP levels via synthesis and breakdown and the allosteric regulation of c-di-GMP over receptor proteins (effectors) and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00422-15

    authors: Rotem O,Nesper J,Borovok I,Gorovits R,Kolot M,Pasternak Z,Shin I,Glatter T,Pietrokovski S,Jenal U,Jurkevitch E

    更新日期:2015-08-31 00:00:00

  • The poc locus is required for 1,2-propanediol-dependent transcription of the cobalamin biosynthetic (cob) and propanediol utilization (pdu) genes of Salmonella typhimurium.

    abstract::In this communication we present evidence that indicates that 1,2-propanediol (1,2-PDL) is a positive effector of the transcription of the cobalamin (vitamin B12) biosynthetic (cob) operon and of the 1,2-PDL utilization (pdu) genes. The stimulatory effects of 1,2-PDL were demonstrated using Mu d-lac transcriptional fu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.7.2267-2272.1992

    authors: Rondon MR,Escalante-Semerena JC

    更新日期:1992-04-01 00:00:00

  • Regulation and timing of deoxyribonucleic acid synthesis in hyphae of Aspergillus nidulans.

    abstract::Pulse labeling of deoxyribonucleic acid (DNA) and radioautography have been used to study the effect of growth rate on nuclear replication in Aspergillus nidulans. When conidia were germinated in media supporting a fast growth rate, the radioactive pulse labeled either all of the nuclei in a cell or none of them. At s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.6.2275-2281.1968

    authors: Kessel M,Rosenberger RF

    更新日期:1968-06-01 00:00:00

  • Synthesis of the sigmaD protein is not sufficient to trigger expression of motility functions in Bacillus subtilis.

    abstract::The gene encoding sigmaD, sigD, is transcribed from two promoter regions, the fla/che promoter region in front of the fla/che operon and PsigD directly in front of sigD. If sigmaD is translated from transcripts originating from PsigD, the cell is unable to express motility functions but synthesizes autolysins. Therefo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.9.2942-2946.1999

    authors: Yang DH,von Kalckreuth J,Allmansberger R

    更新日期:1999-05-01 00:00:00

  • Mycoplasma inocuum sp. n., a saprophyte from chickens.

    abstract::Adler, H. E. (University of California, Davis), M. Shifrine, and Herrad Ortmayer. Mycoplasma inocuum sp. n., a saprophyte from chickens. J. Bacteriol. 82:239-240. 1961.-A new species of Mycoplasma was isolated from the infraorbital sinuses of chickens. The organism, a saprophyte, differs from all known mycoplasmas of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.82.2.239-240.1961

    authors: ADLER HE,SHIFRINE M,ORTMAYER H

    更新日期:1961-08-01 00:00:00

  • Regulation of lysine- and lysine-plus-threonine-inhibitable aspartokinases in Bacillus brevis.

    abstract::Further studies on the expression of the two aspartokinase activities in Bacillus bovis are presented. Aspartokinase I (previously shown to be inhibited and repressed by lysine) was found to be repressed by diaminopimelate in the wild-type strain. However, in a mutant unable to convert diaminopimelate to lysine, starv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.142.2.424-432.1980

    authors: Hitchcock MJ,Hodgson B,Linforth JL

    更新日期:1980-05-01 00:00:00

  • Tn5401, a new class II transposable element from Bacillus thuringiensis.

    abstract::A new class II (Tn3-like) transposable element, designated Tn5401, was recovered from a sporulation-deficient variant of Bacillus thuringiensis subsp. morrisoni EG2158 following its insertion into a recombinant plasmid. Sequence analysis of the insert revealed a 4,837-bp transposon with two large open reading frames, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.10.2835-2845.1994

    authors: Baum JA

    更新日期:1994-05-01 00:00:00

  • Analysis of a mutation affecting the specificity domain for prohead binding of the bacteriophage lambda terminase.

    abstract::Genetic studies have identified a specificity domain for prohead binding in the C-terminal 32 amino acids of gpA, the large subunit of bacteriophage lambda terminase (S. Frackman, D. A. Siegele, and M. Feiss, J. Mol. Biol. 180:283-300, 1984). In the present work, an amber mutation, Aam42, in the fifth-to-last codon of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.3.850-856.1992

    authors: Sippy J,Feiss M

    更新日期:1992-02-01 00:00:00

  • Pyrimidine biosynthetic enzymes of Salmonella typhimurium, repressed specifically by growth in the presence of cytidine.

    abstract::The repressive effects of exogenous cytidine on growing cells was examined in a specially constructed strain in which the pool sizes of endogenous uridine 5'-diphosphate and uridine 5'-triphosphate cannot be varied by the addition of uracil and/or uridine to the medium. Five enzymes of the pyrimidine biosynthetic path...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.124.2.764-774.1975

    authors: Kelln RA,Kinahan JJ,Foltermann KF,O'Donovan GA

    更新日期:1975-11-01 00:00:00

  • Kinetic characterization of the WalRKSpn (VicRK) two-component system of Streptococcus pneumoniae: dependence of WalKSpn (VicK) phosphatase activity on its PAS domain.

    abstract::The WalRK two-component system plays important roles in maintaining cell wall homeostasis and responding to antibiotic stress in low-GC Gram-positive bacteria. In the major human pathogen, Streptococcus pneumoniae, phosphorylated WalR(Spn) (VicR) response regulator positively controls the transcription of genes encodi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01690-09

    authors: Gutu AD,Wayne KJ,Sham LT,Winkler ME

    更新日期:2010-05-01 00:00:00

  • Identification of a new gene, molR, essential for utilization of molybdate by Escherichia coli.

    abstract::A mutation in a new gene, molR, prevented the synthesis in Escherichia coli of molybdoenzymes, including the two formate dehydrogenase isoenzymes, nitrate reductase and trimethylamine-N-oxide reductase. This phenotype was suppressed by supplementing the media with molybdate. Thus, the molR mutant was phenotypically si...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.4.2079-2087.1990

    authors: Lee JH,Wendt JC,Shanmugam KT

    更新日期:1990-04-01 00:00:00

  • Complete genome sequence of Erythrobacter litoralis HTCC2594.

    abstract::Erythrobacter litoralis has been known as a bacteriochlorophyll a-containing, aerobic, anoxygenic, phototrophic bacterium. Here we announce the complete genome sequence of E. litoralis HTCC2594, which is devoid of phototrophic potential. E. litoralis HTCC2594, isolated by dilution-to-extinction culturing from seawater...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00026-09

    authors: Oh HM,Giovannoni SJ,Ferriera S,Johnson J,Cho JC

    更新日期:2009-04-01 00:00:00

  • Involvement of a plasmid-encoded type IV secretion system in the plant tissue watersoaking phenotype of Burkholderia cenocepacia.

    abstract::Burkholderia cenocepacia strain K56-2, a representative of the Burkholderia cepacia complex, is part of the epidemic and clinically problematic ET12 lineage. The strain produced plant tissue watersoaking (ptw) on onion tissue, which is a plant disease-associated trait. Using plasposon mutagenesis, mutants in the ptw p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.18.6015-6024.2004

    authors: Engledow AS,Medrano EG,Mahenthiralingam E,LiPuma JJ,Gonzalez CF

    更新日期:2004-09-01 00:00:00

  • Conjugational recombination in resolvase-deficient ruvC mutants of Escherichia coli K-12 depends on recG.

    abstract::ruvC mutants of Escherichia coli appear to lack an activity that resolves Holliday intermediates into recombinant products. Yet, these strains produce close to normal numbers of recombinants in genetic crosses. This recombination proficiency was found to be a function of recG. A "mini-kan" insertion in recG was introd...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.17.5414-5418.1991

    authors: Lloyd RG

    更新日期:1991-09-01 00:00:00

  • Beta-2-linked glucans secreted by fast-growing species of Rhizobium.

    abstract::Fast-growing species of Rhizobium were found to secrete low-molecular-weight beta-2-linked glucans when cultured in synthetic liquid medium. These glucans are quite similar to beta-2-linked glucans produced by species of Agrobacterium. No reducing terminus was detected in these glucans. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.142.1.243-248.1980

    authors: York WS,McNeil M,Darvill AG,Albersheim P

    更新日期:1980-04-01 00:00:00

  • Repair of x-ray-induced deoxyribonucleic acid single-strand breaks in xth mutants of Escherichia coli.

    abstract::An exonuclease III-deficient strain of Escherichia coli K-12, BW2001 (xthA11), was unable to perform rapid repair of X-ray-induced deoxyribonucleic acid single-strand breaks and appeared to have a defect in the priming of the 3'-termini necessary for initiation of repair synthesis at the breaks. This defect cannot be ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.3.1424-1427.1980

    authors: Seeberg E,Steinum AL

    更新日期:1980-03-01 00:00:00

  • Induced sensitization of normal laboratory animals to Brucella abortus endotoxin.

    abstract::A Boivin preparation of Brucella abortus, unlike common enterobacterial endotoxins, failed to depress water intake or increase numbers of hemolysin-producing spleen cells in mice, or to cause delayed inflammatory reactions in rabbit skin. Reactivity to the B. abortus endotoxin was found only in animals which were prev...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.2.286-290.1968

    authors: Freedman HH,Fox AE,Willis RS,Schwartz BS

    更新日期:1968-02-01 00:00:00

  • 16S ribosomal DNA amplification for phylogenetic study.

    abstract::A set of oligonucleotide primers capable of initiating enzymatic amplification (polymerase chain reaction) on a phylogenetically and taxonomically wide range of bacteria is described along with methods for their use and examples. One pair of primers is capable of amplifying nearly full-length 16S ribosomal DNA (rDNA) ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.2.697-703.1991

    authors: Weisburg WG,Barns SM,Pelletier DA,Lane DJ

    更新日期:1991-01-01 00:00:00

  • The integrase of the conjugative transposon Tn916 directs strand- and sequence-specific cleavage of the origin of conjugal transfer, oriT, by the endonuclease Orf20.

    abstract::Orf20 of the conjugative transposon Tn916 was purified as a chimeric protein fused to maltose binding protein (MBP-Orf20). The chimeric protein possessed endonucleolytic activity, cleaving both strands of the Tn916 origin of conjugal transfer (oriT) at several distinct sites and favoring GT dinucleotides. Incubation o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.6.2207-2213.2006

    authors: Rocco JM,Churchward G

    更新日期:2006-03-01 00:00:00

  • Binding specificity of the periplasmic oligopeptide-binding protein from Escherichia coli.

    abstract::The structural properties required for the binding of peptide substrates to the Escherichia coli periplasmic protein involved in oligopeptide transport were surveyed by measuring the ability of different peptides to compete for binding in an equilibrium dialysis assay with the tripeptide Ala-Phe-[3H]Gly. The protein s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.168.2.775-779.1986

    authors: Guyer CA,Morgan DG,Staros JV

    更新日期:1986-11-01 00:00:00

  • A functional leuABCD operon is required for leucine synthesis by the tyrosine-repressible transaminase in Escherichia coli K-12.

    abstract::In Escherichia coli K-12, two enzymes, encoded by ilvE and tyrB, catalyze the amination of 2-ketoisocaproate (2-KIC) to form leucine. Although leucine-requiring derivatives of an ilvE strain that are unable to grow on 2-KIC were expected to have mutations only in tyrB, mapping studies showed that one such mutation was...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.12.3864-3871.1991

    authors: Vartak NB,Liu L,Wang BM,Berg CM

    更新日期:1991-06-01 00:00:00

  • Regulation of the transposase of Tn4652 by the transposon-encoded protein TnpC.

    abstract::Transposition is a DNA reorganization reaction potentially deleterious for the host. The frequency of transposition is limited by the amount of transposase. Therefore, strict regulation of a transposase is required to keep control over the destructive multiplication of the mobile element. We have shown previously that...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.20.6312-6318.1999

    authors: Hõrak R,Kivisaar M

    更新日期:1999-10-01 00:00:00

  • Identification of Nudix hydrolase family members with an antimutator role in Mycobacterium tuberculosis and Mycobacterium smegmatis.

    abstract::Mycobacterium tuberculosis and Mycobacterium smegmatis MutT1, MutT2, MutT3, and Rv3908 (MutT4) enzymes were screened for an antimutator role. Results indicate that both MutT1, in M. tuberculosis and M. smegmatis, and MutT4, in M. smegmatis, have that role. Furthermore, an 8-oxo-guanosine triphosphatase function for Mu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.8.3159-3161.2006

    authors: Dos Vultos T,Blázquez J,Rauzier J,Matic I,Gicquel B

    更新日期:2006-04-01 00:00:00

  • Replication of the broad-host-range plasmid RK2: direct measurement of intracellular concentrations of the essential TrfA replication proteins and their effect on plasmid copy number.

    abstract::The trfA gene of the broad-host-range plasmid RK2 is essential for initiation of plasmid replication. Two related TrfA proteins of 43 and 32 kilodaltons (kDa) are produced by independent translation initiation at two start codons within the trfA open reading frame. These proteins were o overproduced in Escherichia col...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.7.3849-3858.1990

    authors: Durland RH,Helinski DR

    更新日期:1990-07-01 00:00:00

  • Plasmids in avirulent strains of Agrobacterium.

    abstract::Twelve strains of Agrobacterium radiobacter isolated from naturally occurring crown galls or soil were found to be avirulent on sunflower, tomato, Kalanchoe, and carrot. Eleven strains contained plasmids of molecular weights 77 X 10(6) to 182 X 10(6) as determined by electron microscopy. One strain contained only a sm...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.1.76-80.1977

    authors: Merlo DJ,Nester EW

    更新日期:1977-01-01 00:00:00

  • Antigens of Bordetella pertussis. II. Purification of heat-labile toxin.

    abstract::Banerjea, A. (Rocky Mountain Laboratory, Hamilton, Mont.) and J. Munoz. Antigens of Bordetella pertussis. II, Purification of heat-labile toxin. J. Bacteriol. 84:269-274. 1962.-A mild method of separating heat-labile toxin of Bordetella pertussis from other cellular components is described; it consists of absorbing to...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.84.2.269-274.1962

    authors: BANERJEA A,MUNOZ J

    更新日期:1962-08-01 00:00:00

  • Physiological modifications in the production and repair of methyl methane sulfonate-induced breaks in the deoxyribonucleic acid of Escherichia coli K-12.

    abstract::The medium in which Rec(+) strains of Escherichia coli K-12 are grown affected their sensitivity to treatment with methyl methane sulfonate (MMS). Rec(+) cells grown to the stationary phase in glucose-enriched nutrient broth (GNB) were more resistant to MMS than cells grown in nutrient broth (NB). The repair of MMS-in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.114.1.267-272.1973

    authors: Scudiero DA,Friesen BS,Baptist JE

    更新日期:1973-04-01 00:00:00