An Extended Cyclic Di-GMP Network in the Predatory Bacterium Bdellovibrio bacteriovorus.


UNLABELLED:Over the course of the last 3 decades the role of the second messenger cyclic di-GMP (c-di-GMP) as a master regulator of bacterial physiology was determined. Although the control over c-di-GMP levels via synthesis and breakdown and the allosteric regulation of c-di-GMP over receptor proteins (effectors) and riboswitches have been extensively studied, relatively few effectors have been identified and most are of unknown functions. The obligate predatory bacterium Bdellovibrio bacteriovorus has a peculiar dimorphic life cycle, in which a phenotypic transition from a free-living attack phase (AP) to a sessile, intracellular predatory growth phase (GP) is tightly regulated by specific c-di-GMP diguanylate cyclases. B. bacteriovorus also bears one of the largest complement of defined effectors, almost none of known functions, suggesting that additional proteins may be involved in c-di-GMP signaling. In order to uncover novel c-di-GMP effectors, a c-di-GMP capture-compound mass-spectroscopy experiment was performed on wild-type AP and host-independent (HI) mutant cultures, the latter serving as a proxy for wild-type GP cells. Eighty-four proteins were identified as candidate c-di-GMP binders. Of these proteins, 65 did not include any recognized c-di-GMP binding site, and 3 carried known unorthodox binding sites. Putative functions could be assigned to 59 proteins. These proteins are included in metabolic pathways, regulatory circuits, cell transport, and motility, thereby creating a potentially large c-di-GMP network. False candidate effectors may include members of protein complexes, as well as proteins binding nucleotides or other cofactors that were, respectively, carried over or unspecifically interacted with the capture compound during the pulldown. Of the 84 candidates, 62 were found to specifically bind the c-di-GMP capture compound in AP or in HI cultures, suggesting c-di-GMP control over the whole-cell cycle of the bacterium. High affinity and specificity to c-di-GMP binding were confirmed using microscale thermophoresis with a hypothetical protein bearing a PilZ domain, an acyl coenzyme A dehydrogenase, and a two-component system response regulator, indicating that additional c-di-GMP binding candidates may be bona fide novel effectors. IMPORTANCE:In this study, 84 putative c-di-GMP binding proteins were identified in B. bacteriovorus, an obligate predatory bacterium whose lifestyle and reproduction are dependent on c-di-GMP signaling, using a c-di-GMP capture compound precipitation approach. This predicted complement covers metabolic, energy, transport, motility and regulatory pathways, and most of it is phase specific, i.e., 62 candidates bind the capture compound at defined modes of B. bacteriovorus lifestyle. Three of the putative binders further demonstrated specificity and high affinity to c-di-GMP via microscale thermophoresis, lending support for the presence of additional bona fide c-di-GMP effectors among the pulled-down protein repertoire.


J Bacteriol


Journal of bacteriology


Rotem O,Nesper J,Borovok I,Gorovits R,Kolot M,Pasternak Z,Shin I,Glatter T,Pietrokovski S,Jenal U,Jurkevitch E




Has Abstract


2015-08-31 00:00:00














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    pub_type: 杂志文章


    authors: Möllenkvist A,Nordström T,Halldén C,Christensen JJ,Forsgren A,Riesbeck K

    更新日期:2003-04-01 00:00:00

  • Genetic selection and DNA sequences of 4.5S RNA homologs.

    abstract::A general strategy for cloning the functional homologs of an Escherichia coli gene was used to clone homologs of 4.5S RNA from other bacteria. The genes encoding these homologs were selected by their ability to complement a deletion of the gene for 4.5S RNA. DNA sequences of the regions encoding the homologs were dete...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Brown S,Thon G,Tolentino E

    更新日期:1989-12-01 00:00:00

  • Mechanism of chloride elimination from 3-chloro- and 2,4-dichloro-cis,cis-muconate: new insight obtained from analysis of muconate cycloisomerase variant CatB-K169A.

    abstract::Chloromuconate cycloisomerases of bacteria utilizing chloroaromatic compounds are known to convert 3-chloro-cis,cis-muconate to cis-dienelactone (cis-4-carboxymethylenebut-2-en-4-olide), while usual muconate cycloisomerases transform the same substrate to the bacteriotoxic protoanemonin. Formation of protoanemonin req...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kaulmann U,Kaschabek SR,Schlömann M

    更新日期:2001-08-01 00:00:00

  • The extent of migration of the Holliday junction is a crucial factor for gene conversion in Rhizobium etli.

    abstract::Gene conversion, defined as the nonreciprocal transfer of DNA, is one result of homologous recombination. Three steps in recombination could give rise to gene conversion: (i) DNA synthesis for repair of the degraded segment, (ii) Holliday junction migration, leading to heteroduplex formation, and (iii) repair of misma...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Castellanos M,Romero D

    更新日期:2009-08-01 00:00:00

  • oriT sequence of the antibiotic resistance plasmid R100.

    abstract::We present the nucleotide sequence of the oriT region from plasmid R100. Comparison to other IncF plasmids revealed homology around the proposed nick sites as well as conservation of inverted repeated sequences in the nonhomologous region. Three areas showed strong homology (eight of nine nucleotides) to the consensus...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: McIntire SA,Dempsey WB

    更新日期:1987-08-01 00:00:00

  • Synthesis of the sigmaD protein is not sufficient to trigger expression of motility functions in Bacillus subtilis.

    abstract::The gene encoding sigmaD, sigD, is transcribed from two promoter regions, the fla/che promoter region in front of the fla/che operon and PsigD directly in front of sigD. If sigmaD is translated from transcripts originating from PsigD, the cell is unable to express motility functions but synthesizes autolysins. Therefo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Yang DH,von Kalckreuth J,Allmansberger R

    更新日期:1999-05-01 00:00:00


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    journal_title:Journal of bacteriology

    pub_type: 杂志文章



    更新日期:1965-08-01 00:00:00

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    abstract::Neonatal bacterial meningitis continues to be an important cause of mortality and morbidity worldwide. Escherichia coli possessing the K1 capsular polysaccharide is the most common Gram-negative pathogen causing neonatal meningitis. Here we present the complete genome sequence of neonatal meningitis-associated E. coli...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lu S,Zhang X,Zhu Y,Kim KS,Yang J,Jin Q

    更新日期:2011-12-01 00:00:00

  • Oxygen does not directly regulate carotenoid biosynthesis in Rhodopseudomonas capsulata.

    abstract::We examined the role of bacteriochlorophyll synthesis on the regulation of carotenoid synthesis in Rhodopseudomonas capsulata. Strains capable of making bacteriochlorophyll accumulated greater amounts of carotenoids under low oxygen than they did under high oxygen. However, strains unable to produce bacteriochlorophyl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Biel AJ,Marrs BL

    更新日期:1985-06-01 00:00:00

  • Mutagenesis of Neisseria gonorrhoeae: absence of error-prone repair.

    abstract::The lethal and mutagenic effects of various mutagens on Neisseria gonorrhoeae were investigated. Lethality studies demonstrated that N. gonorrhoeae was relatively sensitive to ethyl methanesulfonate, UV light, and methyl methanesulfonate. Although N. gonorrhoeae was readily mutated by ethyl methanesulfonate and N-meth...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Campbell LA,Yasbin RE

    更新日期:1984-10-01 00:00:00

  • Role of plant root exudate and Sym plasmid-localized nodulation genes in the synthesis by Rhizobium leguminosarum of Tsr factor, which causes thick and short roots on common vetch.

    abstract::In a previous paper it was shown that cocultivation of Rhizobium leguminosarum with the plant Vicia sativa subsp. nigra on solid medium causes a changed mode of growth of the plant roots, resulting in thick and short roots (Tsr). The Sym plasmid present in the bacterium appeared to be essential for causing Tsr (A. A. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Van Brussel AA,Zaat SA,Cremers HC,Wijffelman CA,Pees E,Tak T,Lugtenberg BJ

    更新日期:1986-02-01 00:00:00

  • Transcriptional cross talk within the mar-sox-rob regulon in Escherichia coli is limited to the rob and marRAB operons.

    abstract::Bacteria possess multiple mechanisms to survive exposure to various chemical stresses and antimicrobial compounds. In the enteric bacterium Escherichia coli, three homologous transcription factors-MarA, SoxS, and Rob-play a central role in coordinating this response. Three separate systems are known to regulate the ex...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Chubiz LM,Glekas GD,Rao CV

    更新日期:2012-09-01 00:00:00


    abstract::Enger, M. D. (North Dakota State University, Fargo), and B. P. Sleeper. Multiple cellulase system from Streptomyces antibioticus. J. Bacteriol. 89:23-27. 1965.-Starch-block zone electrophoresis was used to isolate five electrophoretically distinct, active cellulolytic components (I to V) from the crude extracellular c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: ENGER MD,SLEEPER BP

    更新日期:1965-01-01 00:00:00

  • Subapical wall synthesis and wall thickening induced by cycloheximide in hyphae of Aspergillus nidulans.

    abstract::Hyphae of Aspergillus nidulans continued to synthesize all the major polysaccharide components of the cell wall when cycloheximide was added to cultures. In the presence of cycloheximide, hyphae did not elongate, but electron microscopy showed that the walls became thicker around the cell. The conclusion that cyclohex...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sternlicht E,Katz D,Rosenberger RF

    更新日期:1973-05-01 00:00:00

  • Unique regulatory mechanism of sporulation and enterotoxin production in Clostridium perfringens.

    abstract::Clostridium perfringens causes gas gangrene and gastrointestinal (GI) diseases in humans. The most common cause of C. perfringens-associated food poisoning is the consumption of C. perfringens vegetative cells followed by sporulation and production of enterotoxin in the gut. Despite the importance of spore formation i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ohtani K,Hirakawa H,Paredes-Sabja D,Tashiro K,Kuhara S,Sarker MR,Shimizu T

    更新日期:2013-06-01 00:00:00

  • Genome sequence of the welan gum-producing strain Sphingomonas sp. ATCC 31555.

    abstract::Sphingomonas sp. strain ATCC 31555 can produce an anionic heteropolysaccharide, welan gum, which shows excellent stability and viscosity retention even at high temperatures. Here we present a 4.0-Mb assembly of its genome sequence. We have annotated 10 coding sequences (CDSs) responsible for the welan gum biosynthesis...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Wang X,Tao F,Gai Z,Tang H,Xu P

    更新日期:2012-11-01 00:00:00

  • Identification of a protein of 15,000 daltons related to isoleucine-valine biosynthesis in Escherichia coli K-12.

    abstract::The effect of the ilvG671, ilvG468, and ilvG603 mutations (phenotype, IlvG+ Valr; formerly ilvO) upon proteins synthesized was determined by infection of irradiated Escherichia coli K-12 cells, using specifically constructed derivatives of lambda dilv phage. These ilvG alleles are similar to the previously studied ilv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Gray JE,Wallen JW,Calhoun DH

    更新日期:1982-07-01 00:00:00

  • Cloning and expression in Escherichia coli of the Alcaligenes eutrophus H16 poly-beta-hydroxybutyrate biosynthetic pathway.

    abstract::The poly-beta-hydroxybutyrate (PHB) biosynthetic pathway from Alcaligenes eutrophus H16 has been cloned and expressed in Escherichia coli. Initially, an A. eutrophus H16 genomic library was constructed by using cosmid pVK102, and cosmid clones that encoded the PHB biosynthetic pathway were sought by assaying for the f...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Slater SC,Voige WH,Dennis DE

    更新日期:1988-10-01 00:00:00

  • Nucleotide sequence and regulation of a gene involved in bile acid 7-dehydroxylation by Eubacterium sp. strain VPI 12708.

    abstract::Eubacterium sp. strain VPI 12708 is an anaerobic intestinal bacterium that has inducible bile acid 7-dehydroxylation activity. At least four new polypeptides were synthesized after addition of primary bile acids to the growth medium. One of these, of molecular weight 27,000 (P-27), was shown to be involved in the 7-de...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Coleman JP,White WB,Lijewski M,Hylemon PB

    更新日期:1988-05-01 00:00:00

  • Oligohistidine tag mutagenesis of the lambda holin gene.

    abstract::Holins are a diverse group of small integral membrane proteins elaborated by bacteriophages to lyse bacterial hosts and effect release of progeny phages in a precisely timed manner. Recently, the holin S gene of phage lambda was overexpressed and the holin protein was purified to homogeneity by means of an oligohistid...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Smith DL,Young R

    更新日期:1998-08-01 00:00:00

  • Highly efficient protoplast transformation system for Streptococcus faecalis and a new Escherichia coli-S. faecalis shuttle vector.

    abstract::A highly efficient protoplast transformation system for Streptococcus faecalis has been developed by systematically optimizing different parameters. Up to 10(6) transformants per micrograms of DNA were consistently obtained within 3 days, and cell wall regeneration of protoplasts was virtually 100%. A systematic searc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Wirth R,An FY,Clewell DB

    更新日期:1986-03-01 00:00:00

  • Coexpression patterns of sigma(B) regulators in Bacillus subtilis affect sigma(B) inducibility.

    abstract::RsbT is an essential component of the pathway that activates the Bacillus subtilis sigma(B) transcription factor in response to physical stress. rsbT is located within an operon that includes the genes for its principal negative regulator (RsbS) and the stress pathway component that it activates (RsbU), as immediate u...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Zhang S,Reeves A,Woodbury RL,Haldenwang WG

    更新日期:2005-12-01 00:00:00

  • Control of enzyme IIscr and sucrose-6-phosphate hydrolase activities in Streptococcus mutans by transcriptional repressor ScrR binding to the cis-active determinants of the scr regulon.

    abstract::In Streptococcus mutans, enzyme II(scr) and sucrose-6-phosphate hydrolase are two important enzymes in the transport and metabolism of dietary sucrose. The scr regulon of S. mutans is composed of three genes, scrA and scrB, which code for enzyme II(scr) and sucrose-6-phosphate hydrolase, respectively, and scrR, which ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Wang B,Kuramitsu HK

    更新日期:2003-10-01 00:00:00

  • Role of the electrochemical proton gradient in genetic transformation of Haemophilus influenzae.

    abstract::The uptake of homologous DNA by Haemophilus influenzae was studied as a function of the proton motive force in completely competent cultures in the pH range of 6 to 8. The composition and magnitude of the proton motive force were varied by using the ionophores valinomycin and nigericin (in the presence of various pota...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Bremer W,Kooistra J,Hellingwerf KJ,Konings WN

    更新日期:1984-03-01 00:00:00

  • Percent charging of transfer ribonucleic acid and levels of ppGpp and pppGpp in dormant and germinated spores of Bacillus megaterium.

    abstract::The levels of transfer ribonucleic acids (tRNAs) specific for 14 amino acids were almost identical in dormant spores and in spores germinated from 6 to 75 min. Germinated spore tRNAs specific for all amino acids tested were between 63 and 93% charged, and there was no significant change in this value from 6 to 75 min ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Setlow P

    更新日期:1974-06-01 00:00:00

  • The bZip transcription factor Cap1p is involved in multidrug resistance and oxidative stress response in Candida albicans.

    abstract::Candida albicans is an opportunistic pathogenic yeast which frequently develops resistance to the antifungal agent fluconazole (FCZ) in patients undergoing long-term therapy. FCZ-resistant strains often display a reduced intracellular FCZ accumulation which correlates with the overexpression of the ATP-binding cassett...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Alarco AM,Raymond M

    更新日期:1999-02-01 00:00:00

  • AmiC functions as an N-acetylmuramyl-l-alanine amidase necessary for cell separation and can promote autolysis in Neisseria gonorrhoeae.

    abstract::Neisseria gonorrhoeae is prone to undergo autolysis under many conditions not conducive to growth. The role of autolysis during gonococcal infection is not known, but possible advantages for the bacterial population include provision of nutrients to a starving population, modulation of the host immune response by rele...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Garcia DL,Dillard JP

    更新日期:2006-10-01 00:00:00

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    abstract::The plasmid-encoded citrate determinant of the Lactococcus lactis subsp. lactis var. diacetylactis NCDO176 was cloned and functionally expressed in a Cit- Escherichia coli K-12 strain. From deletion derivative analysis, a 3.4-kilobase region was identified which encodes the ability to transport citrate. Analysis of pr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: David S,van der Rest ME,Driessen AJ,Simons G,de Vos WM

    更新日期:1990-10-01 00:00:00

  • Complete genome sequence of Paenibacillus mucilaginosus 3016, a bacterium functional as microbial fertilizer.

    abstract::Paenibacillus mucilaginosus is a ubiquitous functional bacterium in microbial fertilizer. Here we report the complete sequence of P. mucilaginosus 3016. Multiple sets of functional genes have been found in the genome. To the best of our knowledge, this is the first announcement about the complete genome sequence of a ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ma M,Wang Z,Li L,Jiang X,Guan D,Cao F,Chen H,Wang X,Shen D,Du B,Li J

    更新日期:2012-05-01 00:00:00


    abstract::Wright, D. N. (Iowa State University, Ames), and W. R. Lockhart. Environmental control of cell composition in Escherichia coli. J. Bacteriol. 89:1026-1031. 1965.-Escherichia coli was grown in continuous culture at various rates in a defined medium with either the carbon or the nitrogen source as rate-limiting substrat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章



    更新日期:1965-04-01 00:00:00