Abstract:
UNLABELLED:Over the course of the last 3 decades the role of the second messenger cyclic di-GMP (c-di-GMP) as a master regulator of bacterial physiology was determined. Although the control over c-di-GMP levels via synthesis and breakdown and the allosteric regulation of c-di-GMP over receptor proteins (effectors) and riboswitches have been extensively studied, relatively few effectors have been identified and most are of unknown functions. The obligate predatory bacterium Bdellovibrio bacteriovorus has a peculiar dimorphic life cycle, in which a phenotypic transition from a free-living attack phase (AP) to a sessile, intracellular predatory growth phase (GP) is tightly regulated by specific c-di-GMP diguanylate cyclases. B. bacteriovorus also bears one of the largest complement of defined effectors, almost none of known functions, suggesting that additional proteins may be involved in c-di-GMP signaling. In order to uncover novel c-di-GMP effectors, a c-di-GMP capture-compound mass-spectroscopy experiment was performed on wild-type AP and host-independent (HI) mutant cultures, the latter serving as a proxy for wild-type GP cells. Eighty-four proteins were identified as candidate c-di-GMP binders. Of these proteins, 65 did not include any recognized c-di-GMP binding site, and 3 carried known unorthodox binding sites. Putative functions could be assigned to 59 proteins. These proteins are included in metabolic pathways, regulatory circuits, cell transport, and motility, thereby creating a potentially large c-di-GMP network. False candidate effectors may include members of protein complexes, as well as proteins binding nucleotides or other cofactors that were, respectively, carried over or unspecifically interacted with the capture compound during the pulldown. Of the 84 candidates, 62 were found to specifically bind the c-di-GMP capture compound in AP or in HI cultures, suggesting c-di-GMP control over the whole-cell cycle of the bacterium. High affinity and specificity to c-di-GMP binding were confirmed using microscale thermophoresis with a hypothetical protein bearing a PilZ domain, an acyl coenzyme A dehydrogenase, and a two-component system response regulator, indicating that additional c-di-GMP binding candidates may be bona fide novel effectors. IMPORTANCE:In this study, 84 putative c-di-GMP binding proteins were identified in B. bacteriovorus, an obligate predatory bacterium whose lifestyle and reproduction are dependent on c-di-GMP signaling, using a c-di-GMP capture compound precipitation approach. This predicted complement covers metabolic, energy, transport, motility and regulatory pathways, and most of it is phase specific, i.e., 62 candidates bind the capture compound at defined modes of B. bacteriovorus lifestyle. Three of the putative binders further demonstrated specificity and high affinity to c-di-GMP via microscale thermophoresis, lending support for the presence of additional bona fide c-di-GMP effectors among the pulled-down protein repertoire.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Rotem O,Nesper J,Borovok I,Gorovits R,Kolot M,Pasternak Z,Shin I,Glatter T,Pietrokovski S,Jenal U,Jurkevitch Edoi
10.1128/JB.00422-15subject
Has Abstractpub_date
2015-08-31 00:00:00pages
127-37issue
1eissn
0021-9193issn
1098-5530pii
JB.00422-15journal_volume
198pub_type
杂志文章abstract::A new bacteriocin, termed lactococcin A (LCN-A), from Lactococcus lactis subsp. cremoris LMG 2130 was purified and sequenced. The polypeptide contained no unusual amino acids and showed no significant sequence similarity to other known proteins. Only lactococci were killed by the bacteriocin. Of more than 120 L. lacti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.12.3879-3887.1991
更新日期:1991-06-01 00:00:00
abstract::The cis-acting regulatory regions of the tps and ops genes of Myxococcus xanthus were localized by analyzing the expression of fusions of these genes with the lacZ gene. A 201-base-pair (bp) fragment of tps DNa extending 95 bp upstream (-95) from the transcriptional start was sufficient to direct developmentally regul...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.10.4931-4938.1988
更新日期:1988-10-01 00:00:00
abstract::Streptococcus bovis JB1 cells energized with glucose transported glutamine at a rate of 7 nmol/mg of protein per min at a pH of 5.0 to 7.5; sodium had little effect on the transport rate. Because valinomycin-treated cells loaded with K and diluted into Na (pH 6.5) to create an artificial delta psi took up little gluta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.6.2981-2985.1989
更新日期:1989-06-01 00:00:00
abstract::Sequences essential to the chloramphenicol-inducible expression of cat-86, a chloramphenicol acetyltransferase gene, reside in a 144-base pair (bp) regulatory region that intervenes between the cat-86 coding sequence and its promoter. A key regulatory element within the 144-bp segment consists of a pair of inverted-re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.2.696-703.1985
更新日期:1985-11-01 00:00:00
abstract::Many gram-negative bacteria harbor a copper/zinc-containing superoxide dismutase (CuZnSOD) in their periplasms. In pathogenic bacteria, one role of this enzyme may be to protect periplasmic biomolecules from superoxide that is released by host phagocytic cells. However, the enzyme is also present in many nonpathogens ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00554-06
更新日期:2006-09-01 00:00:00
abstract::The Put3p and Gal4p transcriptional activators are members of a distinct class of fungal regulators called the Cys(6) Zn(II)(2) binuclear cluster family. This family includes over 50 different Saccharomyces cerevisiae proteins that share a similar domain organization. Gal4p activates the genes of the galactose utiliza...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.13.3748-3753.2000
更新日期:2000-07-01 00:00:00
abstract::A Synechocystis sp. strain PCC 6803 mutant lacking CtaI, a main subunit of cytochrome c oxidase, is not capable of growing at light intensities below 5 micromol photons m(-2) s(-1), presumably due to an overreduced plastoquinone pool in the thylakoid membrane. Upon selection for growth at light intensities below 5 mic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.4.1286-1294.2006
更新日期:2006-02-01 00:00:00
abstract::A leucine-requiring, rifampin-sensitive strain of Neisseria gonorrhoeae was transformed to a leucine-nonrequiring, rifampin-resistant phenotype with deoxyribonucleic acid (DNA) obtained from both N. meningitidis and N. gonorrhoeae. The transforming efficiency of the meningococcal DNA was about 10- to 100-fold less tha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.2.471-474.1975
更新日期:1975-02-01 00:00:00
abstract::Farrar, W. Edmund, Jr. (Walter Reed Army Institute of Research, Washington, D.C.), Thomas H. Kent, and Van B. Elliott. Lethal gram-negative bacterial superinfection in guinea pigs given bacitracin. J. Bacteriol. 92:496-501. 1966.-Oral administration of a single dose of bacitracin (either 2,000 or 10,000 units) was let...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.2.496-501.1966
更新日期:1966-08-01 00:00:00
abstract::Proteus rettgeri and Escherichia coli W were shown to express structurally different penicillin G acylases. The enzymes had similar substrate specificity but differed in molecular weight, isoelectric point, and electrophoretic mobility in polyacrylamide gels and did not antigenically cross-react. When the organisms we...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.163.3.925-932.1985
更新日期:1985-09-01 00:00:00
abstract::Mycoplasma mobile has a unique mechanism that enables it to glide on solid surfaces faster than any other gliding mycoplasma. To elucidate the gliding mechanism, we developed a transformation system for M. mobile based on a transposon derived from Tn4001. Modification of the electroporation conditions, outgrowth time,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01418-13
更新日期:2014-05-01 00:00:00
abstract::The lic3 locus of Haemophilus influenzae consists of four open reading frames. The derived amino acid sequences of orf2 and orf4 exhibit homology to Escherichia coli GalE and AdK, respectively. The functions of orf1 and orf3 remain unknown. orf1 contains multiple tandem repeats of the tetrameric DNA sequence CAAT near...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.22.7245-7252.1992
更新日期:1992-11-01 00:00:00
abstract::Polyamines are present in high concentrations in archaea, yet little is known about their synthesis, except by extrapolation from bacterial and eucaryal systems. S-Adenosylmethionine (AdoMet) decarboxylase, a pyruvoyl group-containing enzyme that is required for spermidine biosynthesis, has been previously identified ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.23.6667-6672.2000
更新日期:2000-12-01 00:00:00
abstract::The mode of synthesis and the regulation of fructose-1,6-bisphosphatase (Fbpase), a gluconeogenic enzyme, and phosphofructokinase (PFK), a glycolytic enzyme, were investigated in Saccharomyces cerevisiae after growth in the presence of different concentrations of glucose or various gluconeogenic carbon sources. The ac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.2.647-656.1978
更新日期:1978-11-01 00:00:00
abstract::The leucine auxotroph Escherichia coli 2961 exhibited stringent control of net ribonucleic acid (RNA) synthesis during amino acid starvation. After leucine was exhausted from the medium, the rate of uracil incorporation into RNA rapidly decreased to 2 to 4% of the prestarvation value. Infection of the starved cells wi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.1.111-116.1968
更新日期:1968-07-01 00:00:00
abstract::Faine, S. (University of Sydney, Sydney, Australia), and J. van der Hoeden. Virulence-linked colonial and morphological variation in Leptospira. J. Bacteriol. 88:1493-1496. 1964.-Large-colony typical hooked Leptospira icterohaemorrhagiae was virulent for hamsters and guinea pigs. On cultivation, it was gradually repla...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.5.1493-1496.1964
更新日期:1964-11-01 00:00:00
abstract::Two-dimensional gel analyses were made of proteins synthesized in Escherichia coli during various O2- -generating conditions. Nine proteins were constitutively synthesized over wild-type levels in superoxide dismutase (sodA sodB) double mutants. Addition of redox cycling agents such as paraquat and plumbagin at variou...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.3.1476-1484.1989
更新日期:1989-03-01 00:00:00
abstract::The serotype 5 capsule gene cluster of Staphylococcus aureus comprises 16 genes (cap5A through cap5P), but little is known about how the putative gene products function in capsule biosynthesis. We propose that the N-acetylmannosaminuronic acid (ManNAcA) component of the S. aureus serotype 5 capsular polysaccharide (CP...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.16.4818-4824.1999
更新日期:1999-08-01 00:00:00
abstract::Extracellular muramidase-2 of Enterococcus hirae ATCC 9790 was purified to homogeneity by substrate binding, guanidine-HCl extraction, and reversed-phase chromatography. A monoclonal antibody, 2F8, which specifically recognizes muramidase-2, was used to screen a genomic library of E. hirae ATCC 9790 DNA in bacteriopha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.5.1619-1625.1992
更新日期:1992-03-01 00:00:00
abstract::NBU1 is an integrated Bacteroides element that can he mobilized from Bacteroides donors to Bacteroides recipients. Previous studies have shown that a plasmid carrying the internal mobilization region of NBU1 could be transferred by conjugation from Bacteroides thetaiotaomicron to Escherichia coli. In this report, we s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.12.3601-3607.1996
更新日期:1996-06-01 00:00:00
abstract::5-methylcytosine in chromosomal DNA represents a potential source of frequent spontaneous mutation for hyperthermophiles. To determine the relevance of this threat for the archaeon Sulfolobus acidocaldarius, the mode of GGCC methylation by its restriction-modification system, SuaI, was investigated. Distinct isoschizo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.15.4657-4661.2003
更新日期:2003-08-01 00:00:00
abstract::The type IV pili of plasmid R64 belonging to the type IVB group are required only for liquid mating. They consist of the major and minor components PilS pilin and PilV adhesin, respectively. PilS pilin is first synthesized as a 22-kDa prepilin from the pilS gene and is then processed to a 19-kDa mature pilin by PilU p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01204-07
更新日期:2008-02-01 00:00:00
abstract::Freundlich, Martin (University of Minnesota, Minneapolis) and Herman C. Lichstein. Tryptophanase-tryptophan synthetase systems in Escherichia coli. III. Requirements for enzyme synthesis. J. Bacteriol. 84:996-1006. 1962.-The requirements for the formation of tryptophanase and tryptophan synthetase in Escherichia coli ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.5.996-1006.1962
更新日期:1962-11-01 00:00:00
abstract::Escherichia coli W3110 contains four types of IS1 elements in the chromosome. Using an insertion element entrapping system, we collected 116 IS1 plasmid insertion mutants, which resulted from a minimum of 26 independent IS1 insertion events. All of them had insertions of IS1 of the IS1A (IS1E and IS1G) type. Inspectio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.19.5279-5283.1998
更新日期:1998-10-01 00:00:00
abstract::A mutant of Escherichia coli resistant to serine hydroxamate which has a large increase in Km for serine of seryl-tRNA synthetase is described. The mutant serS gene was cloned and sequenced and was found to contain a single-base-pair mutation, resulting in the substitution of the residue alanine 262 by valine in motif...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.11.3347-3350.1995
更新日期:1995-06-01 00:00:00
abstract::Kawakami, Masaya (Gunma University, Maebashi, Japan), Nobutaka Osawa, and Susumu Mitsuhashi. Experimental salmonellosis. VII. Comparison of the immunizing effect of live vaccine and materials extracted from Salmonella enteritidis. J. Bacteriol. 92:1585-1589. 1966.-Attempts were made to isolate the factors of live vacc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.6.1585-1589.1966
更新日期:1966-12-01 00:00:00
abstract::Spores of all strains of Dictyostelium discoideum tested in this study germinated after a heat shock of 45 C for 30 min. Whereas the strains differed in their rates of germination, the rate for each strain was constant. A correlation existed between the rate of germination and the rate of vegetative growth when spores...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.5.1690-1695.1968
更新日期:1968-11-01 00:00:00
abstract::The ultrastructure of the cytoplasmic membrane and cell wall of two strains of Escherichia coli, Proteus morganii, P. vulgaris, Acinetobacter anitratum, Moraxella lacunata, Erwinia amylovora, Acinetobacter sp., and of a plant pathogen, unclassified gram-negative, fixed by the Ryter-Kellenberger procedure, was found to...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.2.953-962.1973
更新日期:1973-02-01 00:00:00
abstract::The nucleotide sequence of a 23S rRNA gene of Campylobacter coli VC167 was determined. The primary sequence of the C. coli 23S rRNA was deduced, and a secondary-structure model was constructed. Comparison with Escherichia coli 23S rRNA showed a major difference in the C. coli rRNA at approximately position 1170 (E. co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.15.4597-4609.1994
更新日期:1994-08-01 00:00:00
abstract::Clusters of cytoplasmic vesicles resembling those in growing hyphal apices of mycelial fungi are found near the tips of buds in three heterobasidiomycetous yeasts, Rhodotorula glutinis, Candida scottii, and Sporobolomyces salmonicolor. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.109.2.922-926.1972
更新日期:1972-02-01 00:00:00