Characterization of the enzymatic component of Clostridium perfringens iota-toxin.

abstract：:Two arginyl-transfer ribonucleic acid (tRNA) synthetase (EC 6.1.1.13, arginine: ribonucleic acid ligase adenosine monophosphate) activities were found in extracts of Escherichia coli strains AB1132 and NP2. The two arginyl-tRNA synthetase activities in extracts of strain AB1132 were found to be separable by diethylami...

journal_title：Journal of bacteriology

authors： Yem DW,Williams LS

更新日期：1973-02-01 00:00:00

abstract：:A naturally occurring constitutive macrolide-lincosamide-streptogramin B (MLS) resistance plasmid, pNE131, from Staphylococcus epidermidis was chosen to study the molecular basis of constitutive expression. Restriction and functional maps of pNE131 are presented along with the nucleotide sequence of ermM, the gene whi...

journal_title：Journal of bacteriology

authors： Lampson BC,Parisi JT

更新日期：1986-05-01 00:00:00

abstract：:Phytoplasmas are plant-pathogenic bacteria that cause numerous diseases. This study shows a strong positive selection on the phytoplasma antigenic membrane protein (Amp). The ratio of nonsynonymous to synonymous substitutions was >1 with all the methods we tested. The clear positive selections imply an important biolo...

journal_title：Journal of bacteriology

authors： Kakizawa S,Oshima K,Jung HY,Suzuki S,Nishigawa H,Arashida R,Miyata S,Ugaki M,Kishino H,Namba S

更新日期：2006-05-01 00:00:00

abstract：:The DnaK protein of Zymomonas mobilis (DnaKz) was identified and found to be 80% identical to the DnaK protein of Escherichia coli on the basis of the sequence of the N-terminal 21 amino acids. The dnaKz gene was cloned and found to be expressed in a thermosensitive dnaK mutant of Escherichia coli. Expression of the f...

journal_title：Journal of bacteriology

authors： Michel GP

更新日期：1993-05-01 00:00:00

abstract：:The DNA sequence of the central regulatory locus vir of Bordetella pertussis predicts that three gene products, BvgA, BvgB, and BvgC, are encoded. Features of the predicted primary structures of these proteins and their homology to other two-component systems suggest that BvgA is located in the cytoplasm, BvgB is loca...

journal_title：Journal of bacteriology

authors： Stibitz S,Yang MS

更新日期：1991-07-01 00:00:00

abstract：:A total of 303 isolates of Bordetella bronchiseptica recovered from 11 host species were characterized by the electrophoretic mobilities of 15 metabolic enzymes, and 21 distinctive multilocus genotypes (electrophoretic types) were distinguished on the basis of allele profiles at the enzyme loci. The population structu...

journal_title：Journal of bacteriology

authors： Musser JM,Bemis DA,Ishikawa H,Selander RK

更新日期：1987-06-01 00:00:00

abstract：:Fruiting body formation in Myxococcus xanthus involves the aggregation of cells to form mounds and the differentiation of rod-shaped cells into spherical myxospores. The surface of the myxospore is composed of several sodium dodecyl sulfate (SDS)-soluble proteins, the best characterized of which is protein S (Mr, 19,0...

journal_title：Journal of bacteriology

authors： McCleary WR,Esmon B,Zusman DR

更新日期：1991-03-01 00:00:00

abstract：:The type IV pili of plasmid R64 belonging to the type IVB group are required only for liquid mating. They consist of the major and minor components PilS pilin and PilV adhesin, respectively. PilS pilin is first synthesized as a 22-kDa prepilin from the pilS gene and is then processed to a 19-kDa mature pilin by PilU p...

journal_title：Journal of bacteriology

authors： Shimoda E,Muto T,Horiuchi T,Furuya N,Komano T

更新日期：2008-02-01 00:00:00

abstract：:The gene specifying a membrane-bound nucleoside diphosphate sugar hydrolase of Salmonella typhimurium was mapped near the metA locus by using intergeneric crosses between this bacterium and Escherichia coli. ...

journal_title：Journal of bacteriology

authors： Minton N,Gunn J,Beacham IR

更新日期：1979-03-01 00:00:00

abstract：:DNA damage-inducible DNA replication in SOS-induced Escherichia coli cells, termed inducible stable DNA replication (iSDR), has previously been shown to require either the RecBCD or the RecE pathway of homologous recombination for initiation. Here, we demonstrate that recB recC sbcC quadruple mutant cells are capable ...

journal_title：Journal of bacteriology

authors： Asai T,Kogoma T

更新日期：1994-11-01 00:00:00

abstract：:Mutations in the spoIIIA and spoIIIJ loci disrupt the compartmentalization of gene expression during sporulation of Bacillus subtilis. The breakdown in compartmentalization is not the cause of their being blocked in spore formation. Rather, it appears to be a consequence of the engulfed prespore's being unstable. ...

journal_title：Journal of bacteriology

authors： Li Z,Di Donato F,Piggot PJ

更新日期：2004-04-01 00:00:00

abstract：:Virus-host interactions are regulated by complex coevolutionary dynamics. In Streptococcus pneumoniae, phase-variable type I restriction-modification (R-M) systems are part of the core genome. We hypothesized that the ability of the R-M systems to switch between six target DNA specificities also has a key role in prev...

journal_title：Journal of bacteriology

authors： Furi L,Crawford LA,Rangel-Pineros G,Manso AS,De Ste Croix M,Haigh RD,Kwun MJ,Engelsen Fjelland K,Gilfillan GD,Bentley SD,Croucher NJ,Clokie MR,Oggioni MR

更新日期：2019-09-06 00:00:00

abstract：:Tn5 insertion mutations in the recN gene, and in what appears to be a new RecF pathway gene designated recO and mapping at approximately 55.4 min on the standard genetic map, were isolated by screening Tn5 insertion mutations that cotransduced with tyrA. The recO1504::Tn5 mutation decreased the frequency of recombinat...

journal_title：Journal of bacteriology

authors： Kolodner R,Fishel RA,Howard M

更新日期：1985-09-01 00:00:00

abstract：:Plasmid pE194 has been shown to be rescued by integration after cultivation of infected Bacillus subtilis recE4 cells at a restrictive high temperature. The plasmid is also spontaneously excised from the chromosome at a low frequency by precise or imprecise excision (J. Hofemeister, M. Israeli-Reches, and D. Dubnau, M...

journal_title：Journal of bacteriology

authors： Conrad B,Bashkirov VI,Hofemeister J

更新日期：1992-11-01 00:00:00

abstract：:The DNA binding proteins ArgR and AhrC are essential for regulation of arginine metabolism in Escherichia coli and Bacillus subtilis, respectively. A unique property of these regulators is that they form hexameric protein complexes, mediating repression of arginine biosynthetic pathways as well as activation of argini...

journal_title：Journal of bacteriology

authors： Larsen R,Buist G,Kuipers OP,Kok J

更新日期：2004-02-01 00:00:00

abstract：:The specific activities of 13 aminoacyl-soluble ribonucleic acid (sRNA) synthetases were measured at various time intervals during the germination of Botryodiplodia theobromae conidiospores. The enzyme activities were low or absent in ungerminated spores, and they increased rapidly as germination proceeded. When extra...

journal_title：Journal of bacteriology

authors： Van Etten JL,Brambl RM

更新日期：1968-10-01 00:00:00

abstract：:A fragment of the Lactococcus lactis chromosome containing an open reading frame of 265 codons, denoted bglR, has been characterized. The polypeptide encoded by bglR shares 36 to 30% sequence identity with a family of regulatory proteins including ArbG from Erwinia chrysanthemi, BglG from Escherichia coli, and SacT an...

journal_title：Journal of bacteriology

authors： Bardowski J,Ehrlich SD,Chopin A

更新日期：1994-09-01 00:00:00

abstract：:Phosphorylation of a major 57-kilodalton protein substrate was observed in cell lysates of Spiroplasma melliferum BC3 incubated with [gamma-32P]ATP. Only serine phosphates have been isolated from the acid hydrolysate of the phosphorylated protein. The 57-kilodalton protein substrate was found, to a large extent, in th...

journal_title：Journal of bacteriology

authors： Platt MW,Reizer J,Rottem S

更新日期：1990-05-01 00:00:00

abstract：:A mutant of Pseudomonas aeruginosa severely deficient in outer membrane protein F levels was isolated by screening heavily mutagenized strains for membrane protein alterations on sodium dodecyl sulphate-polyacrylamide gel electrophoresis. To provide a basis for phenotypic comparison, three independent spontaneous reve...

journal_title：Journal of bacteriology

authors： Nicas TI,Hancock RE

更新日期：1983-01-01 00:00:00

abstract：:Acid-soluble spore proteins (ASSPs) comprise about 5% of the total protein of mature spores of different Bacillus subtilis strains. They consist of three abundant species, alpha, beta, and gamma, four less abundant species, and several minor species, alpha, beta, and gamma make up about 18, 18 and 36%, respectively, o...

journal_title：Journal of bacteriology

authors： Johnson WC,Tipper DJ

更新日期：1981-06-01 00:00:00

abstract：:Two new mutants of Rhodobacter sphaeroides deficient in sulfolipid accumulation were isolated by directly screening mutagenized cell lines for polar lipid composition by thin-layer chromatography of lipid extracts. A genomic clone which complemented the mutations in these two lines, but not the previously described su...

journal_title：Journal of bacteriology

authors： Benning C,Somerville CR

更新日期：1992-10-01 00:00:00

abstract：:The control of men gene expression during growth and sporulation of Bacillus subtilis was examined at the transcriptional level. Two different approaches were used. (i) Steady-state levels of men-specific mRNA were measured directly. (ii) A men'-lacZ gene fusion was constructed. In both cases, it was observed that men...

journal_title：Journal of bacteriology

authors： Miller P,Mueller J,Hill K,Taber H

更新日期：1988-06-01 00:00:00

abstract：:Despite extensive similarities between the genomes of the Streptomyces temperate phages phiC31 and phiBT1, the attP-int loci are poorly conserved. Here we demonstrate that phiBT1 integrates into a different attachment site than phiC31. phiBT1 attB lies within SCO4848 encoding a 79-amino-acid putative integral membrane...

journal_title：Journal of bacteriology

authors： Gregory MA,Till R,Smith MC

更新日期：2003-09-01 00:00:00

abstract：:The maintenance energy coefficient of Desulfovibrio vulgaris was studied by using a chemostat, with Methanosarcina barkeri or sulfate as the electron acceptor; lithium lactate or sodium pyruvate served as the electron donor. The experiments showed that the growth energetics of D. vulgaris or M. barkeri were greatly af...

journal_title：Journal of bacteriology

authors： Traore AS,Gaudin C,Hatchikian CE,Le Gall J,Belaich JP

更新日期：1983-09-01 00:00:00

abstract：:The relative stability of membrane proteins in Escherichia coli was investigated to determine whether these proteins are degraded at heterogeneous rates and, if so, whether the degradative rates are correlated with the sizes or charges of the proteins. Cells growing in a glucose-limited chemostat with a generation tim...

journal_title：Journal of bacteriology

authors： Schroer DW,St John AC

更新日期：1981-05-01 00:00:00

abstract：:Endoribonuclease RNase E appears to control the rate-limiting step that mediates the degradation of many mRNA species in bacteria. In this work, an RNase E-like activity in Archaea is described. An endoribonucleolytic activity from the extreme halophile Haloarcula marismortui showed the same RNA substrate specificity ...

journal_title：Journal of bacteriology

authors： Franzetti B,Sohlberg B,Zaccai G,von Gabain A

更新日期：1997-02-01 00:00:00

abstract：:The general stress regulon of Bacillus subtilis comprises approximately 200 genes and is under the control of the alternative sigma factor σ(B). The activation of σ(B) occurs in response to multiple physical stress stimuli as well as energy starvation conditions. The expression of the general stress proteins provides ...

journal_title：Journal of bacteriology

authors： Reder A,Höper D,Gerth U,Hecker M

更新日期：2012-07-01 00:00:00

abstract：:Escherichia coli has been used as a platform host for studying the production of free fatty acids (FFA) and other energy-dense compounds useful in biofuel applications. Most of the FFA produced by E. coli are found extracellularly. This finding suggests that a mechanism for transport across the cell envelope exists, y...

journal_title：Journal of bacteriology

authors： Lennen RM,Politz MG,Kruziki MA,Pfleger BF

更新日期：2013-01-01 00:00:00

abstract：:In all organisms, chromosome replication is regulated mainly at the initiation step. Most of the knowledge about the mechanisms that regulate replication initiation in bacteria has come from studies on rod-shaped bacteria, such as Escherichia coli and Bacillus subtilisStreptomyces is a bacterial genus that is characte...

journal_title：Journal of bacteriology

authors： Łebkowski T,Wolański M,Ołdziej S,Flärdh K,Zakrzewska-Czerwińska J

更新日期：2020-01-15 00:00:00

abstract：:ICE6013 represents one of two families of integrative conjugative elements (ICEs) identified in the pan-genome of the human and animal pathogen Staphylococcus aureus Here we investigated the excision and conjugation functions of ICE6013 and further characterized the diversity of this element. ICE6013 excision was not ...

journal_title：Journal of bacteriology

authors： Sansevere EA,Luo X,Park JY,Yoon S,Seo KS,Robinson DA

更新日期：2017-03-28 00:00:00