Abstract:
:Virus-host interactions are regulated by complex coevolutionary dynamics. In Streptococcus pneumoniae, phase-variable type I restriction-modification (R-M) systems are part of the core genome. We hypothesized that the ability of the R-M systems to switch between six target DNA specificities also has a key role in preventing the spread of bacteriophages. Using the streptococcal temperate bacteriophage SpSL1, we show that the variants of both the SpnIII and SpnIV R-M systems are able to restrict invading bacteriophage with an efficiency approximately proportional to the number of target sites in the bacteriophage genome. In addition to restriction of lytic replication, SpnIII also led to abortive infection in the majority of host cells. During lytic infection, transcriptional analysis found evidence of phage-host interaction through the strong upregulation of the nrdR nucleotide biosynthesis regulon. During lysogeny, the phage had less of an effect on host gene regulation. This research demonstrates a novel combined bacteriophage restriction and abortive infection mechanism, highlighting the importance that the phase-variable type I R-M systems have in the multifunctional defense against bacteriophage infection in the respiratory pathogen S. pneumoniaeIMPORTANCE With antimicrobial drug resistance becoming an increasing burden on human health, much attention has been focused on the potential use of bacteriophages and their enzymes as therapeutics. However, the investigations into the physiology of the complex interactions of bacteriophages with their hosts have attracted far less attention, in comparison. This work describes the molecular characterization of the infectious cycle of a bacteriophage in the important human pathogen Streptococcus pneumoniae and explores the intricate relationship between phase-variable host defense mechanisms and the virus. This is the first report showing how a phase-variable type I restriction-modification system is involved in bacteriophage restriction while it also provides an additional level of infection control through abortive infection.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Furi L,Crawford LA,Rangel-Pineros G,Manso AS,De Ste Croix M,Haigh RD,Kwun MJ,Engelsen Fjelland K,Gilfillan GD,Bentley SD,Croucher NJ,Clokie MR,Oggioni MRdoi
10.1128/JB.00370-19subject
Has Abstractpub_date
2019-09-06 00:00:00issue
19eissn
0021-9193issn
1098-5530pii
JB.00370-19journal_volume
201pub_type
杂志文章abstract::The lining of the maltodextrin-specific maltoporin (LamB) channel exhibits a string of aromatic residues, the greasy slide, part of which has been shown previously by crystallography to be involved in substrate binding. To probe the functional role of the greasy slide, alanine scanning mutagenesis has been performed o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.11.2994-2999.2002
更新日期:2002-06-01 00:00:00
abstract::A series of R (rough) Salmonella minnesota mutants with rfb, rfe, and rfa mutations leading to various defects in the biosynthesis of cell wall lipopolysaccharide was analyzed as to their enterobacterial common antigen (CA) content. All mutants that had functional rfe genes were CA(+) as is the wild-type parent. This ...
journal_title:Journal of bacteriology
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doi:10.1128/JB.119.3.760-764.1974
更新日期:1974-09-01 00:00:00
abstract::Energy-coupling factor (ECF) transporters form a large group of vitamin uptake systems in prokaryotes. They are composed of highly diverse, substrate-specific, transmembrane proteins (S units), a ubiquitous transmembrane protein (T unit), and homo- or hetero-oligomeric ABC ATPases. Biotin transporters represent a spec...
journal_title:Journal of bacteriology
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doi:10.1128/JB.00350-13
更新日期:2013-09-01 00:00:00
abstract::The current work by Jain et al. (S. Jain, A. M. Chang, M. Singh, J. S. McLean, et al., J Bacteriol 201:e00683-18, 2019, https://doi.org/10.1128/JB.00683-18) reports the cloning of the lipid A deacylase gene of Porphyromonas gingivalis and the phenotypic characterization of the enzyme. Attempts to clone the gene and th...
journal_title:Journal of bacteriology
pub_type: 评论,杂志文章
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更新日期:2019-05-08 00:00:00
abstract::In cyanobacteria, gene expression is regulated mainly at the level of transcription initiation, which is mediated by the RNA polymerase holoenzyme. The RNA polymerase core is catalytically active, while the sigma factor recognizes promoter sequences. Group 2 sigma factors are similar to the principal sigma factor but ...
journal_title:Journal of bacteriology
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doi:10.1128/JB.00132-09
更新日期:2009-06-01 00:00:00
abstract::Bradyrhizobium diazoefficiens is a soil alphaproteobacterium that possesses two evolutionarily distinct flagellar systems, a constitutive subpolar flagellum and inducible lateral flagella that, depending on the carbon source, may be expressed simultaneously in liquid medium and used interactively for swimming. In each...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00708-19
更新日期:2020-02-11 00:00:00
abstract::Silverman, Sidney J. (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.), Lynn P. Elwell, and James F. Drawdy. Influence of route of infection and other factors on growth and distribution of Listeria monocytogenes in organs of mice. J. Bacteriol. 86:355-362. 1963.-The growth of Listeria monocytogenes in ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.3.355-362.1963
更新日期:1963-09-01 00:00:00
abstract::L-Glutamate decarboxylase (GAD) activity of Clostridium perfringens (ATCC 8009) cells grown in various culture conditions was investigated. Remarkable variations of GAD level occur during the growth cycle in thioglycollate broth. These changes are affected by the pH of the culture medium. Addition of alkali to the cul...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.123.3.1115-1123.1975
更新日期:1975-09-01 00:00:00
abstract::Desulfomonile tiedjei DCB-1, a sulfate-reducing bacterium, conserves energy for growth from reductive dehalogenation of 3-chlorobenzoate by an uncharacterized chemiosmotic process. Respiratory electron transport components were examined in D. tiedjei cells grown under conditions for reductive dehalogenation, pyruvate ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.1.40-46.1999
更新日期:1999-01-01 00:00:00
abstract::The ultrastructure of the cytoplasmic membrane and cell wall of two strains of Escherichia coli, Proteus morganii, P. vulgaris, Acinetobacter anitratum, Moraxella lacunata, Erwinia amylovora, Acinetobacter sp., and of a plant pathogen, unclassified gram-negative, fixed by the Ryter-Kellenberger procedure, was found to...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.2.953-962.1973
更新日期:1973-02-01 00:00:00
abstract::Freeman, B. M. (Houghton Poultry Research Station, Huntingdon, England), and L. G. Chubb. Effect of Salmonella gallinarum on certain Krebs cycle intermediates of domestic fowl. J. Bacteriol. 88:93-95. 1964.-In chickens infected with Salmonella gallinarum, the citric acid content of the blood rose but that of the liver...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.1.93-95.1964
更新日期:1964-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.1.240-248.2006
更新日期:2006-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.19.6298-6302.1992
更新日期:1992-10-01 00:00:00
abstract::Cytochrome bd oxidase operons from more than 50 species of bacteria contain a short gene encoding a small protein that ranges from ∼30 to 50 amino acids and is predicted to localize to the cell membrane. Although cytochrome bd oxidases have been studied for more than 70 years, little is known about the role of this sm...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00324-13
更新日期:2013-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.110.3.803-808.1972
更新日期:1972-06-01 00:00:00
abstract::Superoxide dismutase (SOD) and catalase (CAT) levels were determined for several aerobically grown halophilic vibrios and compared with those found in aerobically grown Escherichia coli K-12. The SOD levels ranged from 25 to 103.6 U/mg of protein for the vibrios compared with 44.6 U/mg of protein for E. coli. The CAT ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.134.2.375-380.1978
更新日期:1978-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.24.8018-8023.1993
更新日期:1993-12-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1974-01-01 00:00:00
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abstract::Melanin production is a major virulence factor for Cryptococcus neoformans, an organism causing life-threatening infections in an estimated 10% of AIDS patients. In order to characterize the events involved in melanin synthesis, an enzyme having diphenol oxidase activity was purified and its gene was cloned. The enzym...
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更新日期:1994-02-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1968-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2006-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.20.6238-6246.1999
更新日期:1999-10-01 00:00:00
abstract::Anabaena variabilis ATCC 29413 is a heterotrophic, nitrogen-fixing cyanobacterium that has been reported to fix nitrogen and reduce acetylene to ethane in the absence of molybdenum. DNA from this strain hybridized well at low stringency to the nitrogenase 2 (vnfDGK) genes of Azotobacter vinelandii. The hybridizing reg...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.19.6276-6286.1993
更新日期:1993-10-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1999-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.3.941-947.1982
更新日期:1982-03-01 00:00:00
abstract::Calcium-dependent exonuclease activity was produced by sporulating cells of Bacillus subtilis 168. Nuclease activity was released into the culture medium at approximately the same time as sporulation proteases, and production of these enzymes was tied to DNA replication. Results suggest that nuclease production is a f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.2.768-770.1982
更新日期:1982-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.1.99-103.1962
更新日期:1962-07-01 00:00:00
abstract::Mutations in carbohydrate-negative mutants of Pseudomonas aeruginosa PAO1 individually deficient in glucose 6-phosphate dehydrogenase (zwf), 6-phosphogluconate dehydratase (edd), or pyruvate carboxylase (pyc) were mapped on the chromosome by plasmid R68.45-mediated conjugation and by bacteriophage F116L-mediated trans...
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pub_type: 杂志文章
doi:10.1128/JB.156.3.1123-1129.1983
更新日期:1983-12-01 00:00:00