Abstract:
:The nucleic acid binding cold shock proteins (CSPs) and the cold-induced DEAD box RNA helicases have been proposed separately to act as RNA chaperones, but no experimental evidence has been reported on a direct cooperation. To investigate the possible interaction of the putative RNA helicases CshA and CshB and the CSPs from Bacillus subtilis during cold shock, we performed genetic as well as fluorescence resonance energy transfer (FRET) experiments. Both cshA and cshB genes could be deleted only in the presence of a cshB copy in trans, showing that the presence of one csh gene is essential for viability. The combined gene deletion of cshB and cspD resulted in a cold-sensitive phenotype that was not observed for either helicase or csp single mutants. In addition to the colocalization of the putative helicases CshA and CshB with CspB and the ribosomes in areas surrounding the nucleoid, we detected a strong FRET interaction in vivo between CshB and CspB that depended on active transcription. In contrast, a FRET interaction was not observed for CshB and the ribosomal protein L1. Therefore, we propose a model in which the putative cold-induced helicases and the CSPs work in conjunction to rescue misfolded mRNA molecules and maintain proper initiation of translation at low temperatures in B. subtilis.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Hunger K,Beckering CL,Wiegeshoff F,Graumann PL,Marahiel MAdoi
10.1128/JB.188.1.240-248.2006keywords:
subject
Has Abstractpub_date
2006-01-01 00:00:00pages
240-8issue
1eissn
0021-9193issn
1098-5530pii
188/1/240journal_volume
188pub_type
杂志文章abstract::Pseudomonas cepacia mutants deficient in either 6-phosphogluconate (6PGA) dehydratase (Edd-) or 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase (Eda-) failed to utilize glucose or gluconate despite the prominence of of 6-phosphogluconate dehydrogenase (6PGAD) ii this bacterium and the potential for utilizing the pen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.150.3.1340-1347.1982
更新日期:1982-06-01 00:00:00
abstract::The growth phase-dependent change in sucrose density gradient centrifugation patterns of ribosomes was analyzed for both laboratory strains of Escherichia coli and natural isolates from the ECOR collection. All of the natural isolates examined formed 100S ribosome dimers in the stationary phase, and ribosome modulatio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.10.2893-2899.2000
更新日期:2000-05-01 00:00:00
abstract::A novel method is described that allows a direct determination of the location of a portion of the dipicolinic acid (DPA) in spores. Ultraviolet (UV) irradiation is shown to cause cross-linkage of DPA to spore proteins which have the characteristics of membrane proteins. We suggest that DPA resides in the inner foresp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.118.1.202-208.1974
更新日期:1974-04-01 00:00:00
abstract::The protein SpoIVB plays a key role in signaling in the final sigma(K) checkpoint of Bacillus subtilis. This regulatory mechanism coordinates late gene expression during development in this organism and we have recently shown SpoIVB to be a serine peptidase. SpoIVB signals by transiting a membrane, undergoing self-cle...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.1.191-199.2002
更新日期:2002-01-01 00:00:00
abstract::A comprehensive equation, upsilon = VM/[1 + (A0.5/Fru-P2)n] [ 1 + (Glc-6-P/I0.5)], has been proposed to represent the quantitative interrelationships between the rate of glucose utilization and the levels of glucose-6-phosphate and fructose-1,6-diphosphate in the intact Escherichia coli cell. This comprehensive equati...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.128.1.165-169.1976
更新日期:1976-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.20.6095-6106.2001
更新日期:2001-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.183.18.5279-5284.2001
更新日期:2001-09-01 00:00:00
abstract::The RecA proteins of the unusually strong protease-constitutive mutants recA1202 and recA1211 can use RNA in addition to single-stranded DNA (ssDNA) as a cofactor in the cleavage of the LexA repressor in vitro. In the presence of rRNA or tRNA, the effectiveness of these proteins decreased in the order RecA1202 greater...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.10.4823-4827.1988
更新日期:1988-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.10.6112-6121.1990
更新日期:1990-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.3.1245-1253.1988
更新日期:1988-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.7.2010-2017.2000
更新日期:2000-04-01 00:00:00
abstract::The isocitrate lyase from a thermophilic Bacillus is activated about threefold by a variety of salts. Such strong stimulation of activity is not seen with isocitrate lyase from the mesophiles, Bacillus licheniformis, Bacillus megaterium, Escherichia coli, and Aspergillus nidulans. The salt activation is markedly pH-de...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.116.3.1160-1169.1973
更新日期:1973-12-01 00:00:00
abstract::In many organisms, phosphatase expression and phosphate (P) uptake are coordinately regulated by the Pho regulon. In Myxococcus xanthus P limitation initiates multicellular development, a process associated with changes in phosphatase expression. We sought here to characterize the link between P acquisition and develo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01781-07
更新日期:2008-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01477-12
更新日期:2013-01-01 00:00:00
abstract::As a result of serial exposures to a mutagenic agent, N-methyl-N'-nitro-N-nitrosoguanidine, the yield of enterotoxin A produced by the last mutant in the series was increased nearly 20-fold over the amount produced by the parent Staphylococcus aureus 100. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.106.1.289-291.1971
更新日期:1971-04-01 00:00:00
abstract::The genome of the facultative ribulose monophosphate (RuMP) cycle methylotroph Bacillus methanolicus encodes two bisphosphatases (GlpX), one on the chromosome (GlpX(C)) and one on plasmid pBM19 (GlpX(P)), which is required for methylotrophy. Both enzymes were purified from recombinant Escherichia coli and were shown t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00672-13
更新日期:2013-11-01 00:00:00
abstract::The photosynthetic bacterium Rhodobacter capsulatus contains two [NiFe]hydrogenases: an energy-generating hydrogenase, HupSL, and a regulatory hydrogenase, HupUV. The synthesis of HupSL is specifically activated by H(2) through a signal transduction cascade comprising three proteins: the H(2)-sensing HupUV protein, th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.24.7111-7119.2003
更新日期:2003-12-01 00:00:00
abstract::In Caulobacter crescentus, progression through the cell cycle is governed by the periodic activation and inactivation of the master regulator CtrA. Two phosphorelays, each initiating with the histidine kinase CckA, promote CtrA activation by driving its phosphorylation and by inactivating its proteolysis. Here, we exa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00992-09
更新日期:2009-12-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2019-06-21 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.19.6358-6363.1993
更新日期:1993-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00192-11
更新日期:2011-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01868-12
更新日期:2012-12-01 00:00:00
abstract::The ccd mechanism specified by the ccdA and ccdB genes of the mini-F plasmid determines fate of plasmid-free segregants in Escherichia coli (Jaffé et al., J. Bacteriol. 163:841-849, 1985). The killing function in plasmid-free segregants by the ccd mechanism did not affect cell growth of coexisting cells in the same cu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.1.100-104.1986
更新日期:1986-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.1.256-263.1979
更新日期:1979-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.146.1.192-199.1981
更新日期:1981-04-01 00:00:00
abstract::CheY is the response regulator in the signal transduction pathway of bacterial chemotaxis. Position 106 of CheY is occupied by a conserved aromatic residue (tyrosine or phenylalanine) in the response regulator superfamily. A number of substitutions at position 106 have been made and characterized by both behavioral an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.14.4208-4215.1996
更新日期:1996-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.24.7067-7069.2000
更新日期:2000-12-01 00:00:00
abstract::Lipopolysaccharide (LPS) fractions were isolated from three species of blue-green algae of the genus Phormidium, namely, P. africanum, P. laminosum, and P. uncinatum, by using a phenol-water procedure followed by exhaustive extraction with ammonium oxalate. The materials obtained were shown to be closely related bioch...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.1.1-3.1977
更新日期:1977-04-01 00:00:00
abstract::Recent studies of anaerobic toluene catabolism have demonstrated a novel reaction for anaerobic hydrocarbon activation: the addition of the methyl carbon of toluene to fumarate to form benzylsuccinate. In vitro studies of the anaerobic benzylsuccinate synthase reaction indicate that the H atom abstracted from the tolu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.20.5454-5457.1998
更新日期:1998-10-01 00:00:00
abstract::Myxococcus xanthus multicellular fruiting body development is initiated by nutrient limitation at high cell density. Five clustered point mutations (sasB5, -14, -15, -16, and -17) can bypass the starvation and high-cell-density requirements for expression of the 4521 developmental reporter gene. These mutants express ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00