Identification and expression of the Escherichia coli fdhD and fdhE genes, which are involved in the formation of respiratory formate dehydrogenase.

abstract：:The M-band technique was used to assess the number of attachment points of DNA to the cell membrane of Streptococcus faecalis grown at three different rates. Cells were X irradiated in liquid nitrogen and then analyzed simultaneously for the introduction of double-strand breaks into the chromosome and the degree of re...

journal_title：Journal of bacteriology

authors： Parks LC,Rigney D,Daneo-Moore L,Higgins ML

更新日期：1982-10-01 00:00:00

abstract：:The Staphylococcus aureus autoinducer-2 (AI-2) producer protein LuxS is phosphorylated by the Ser/Thr kinase Stk1 at a unique position, Thr14. The enzymatic activity of the phosphorylated isoform of LuxS was abrogated compared to that of nonphosphorylated LuxS, thus providing the first evidence of an AI-2-producing en...

journal_title：Journal of bacteriology

authors： Cluzel ME,Zanella-Cléon I,Cozzone AJ,Fütterer K,Duclos B,Molle V

更新日期：2010-12-01 00:00:00

abstract：:Transcription-coupled repair (TCR) is a cellular process by which some forms of DNA damage are repaired more rapidly from transcribed strands of active genes than from nontranscribed strands or the overall genome. In humans, the TCR coupling factor, CSB, plays a critical role in restoring transcription following both ...

journal_title：Journal of bacteriology

authors： Schalow BJ,Courcelle CT,Courcelle J

更新日期：2012-05-01 00:00:00

abstract：:Borrelia burgdorferi is a motile spirochete which has been identified as the causative microorganism in Lyme disease. The physiological functions which govern the motility of this organism have not been elucidated. In this study, we found that motility of B. burgdorferi required an environment similar to interstitial ...

journal_title：Journal of bacteriology

authors： Shi W,Yang Z,Geng Y,Wolinsky LE,Lovett MA

更新日期：1998-01-01 00:00:00

abstract：:The genome of Corynebacterium glutamicum type strain ATCC 13032 (accession number BX927147) contains three prophages, CGP1, CGP2, and CGP3. We recently observed that many genes within the CGP3 prophage region have increased mRNA levels in a dtxR deletion mutant that lacks the master regulator of iron homeostasis (J. W...

journal_title：Journal of bacteriology

authors： Frunzke J,Bramkamp M,Schweitzer JE,Bott M

更新日期：2008-07-01 00:00:00

abstract：:In exponentially growing cultures of Escherichia coli strains carrying the dnaC28 mutation, DNA replication can be synchronized by temperature changes (R. L. Rodriguez, M. S. Dalbey, and C. I. Davern, J. Mol. Biol. 74:599-604, 1973). We used this synchronization procedure and DNA-DNA hybridization to develop a techniq...

journal_title：Journal of bacteriology

authors： Fayet O,Prere MF

更新日期：1987-12-01 00:00:00

abstract：:Galactoside permease-containing cells of Escherichia coli can be depleted of energy reserves so that the "downhill" cellular hydrolysis of o-nitrophenyl-beta-d-galactopyranoside (ONPG) no longer takes place. Treatment of such energy-depleted cells with proton-conducting agents such as carbonylcyanide m-chlorophenylhyd...

journal_title：Journal of bacteriology

authors： Cecchini G,Koch AL

更新日期：1975-07-01 00:00:00

abstract：:Microcin J25 (MccJ25) is a small peptide antibiotic produced by an Escherichia coli strain isolated from human feces. The genetic determinants for MccJ25 synthesis and immunity have been cloned from the low-copy-number wild-type plasmid pTUC1OO into the compatible vectors pBR322 and pACYC184. Physical and phenotypical...

journal_title：Journal of bacteriology

authors： Solbiati JO,Ciaccio M,Farías RN,Salomón RA

更新日期：1996-06-01 00:00:00

abstract：:Recent studies of anaerobic toluene catabolism have demonstrated a novel reaction for anaerobic hydrocarbon activation: the addition of the methyl carbon of toluene to fumarate to form benzylsuccinate. In vitro studies of the anaerobic benzylsuccinate synthase reaction indicate that the H atom abstracted from the tolu...

journal_title：Journal of bacteriology

authors： Beller HR,Spormann AM

更新日期：1998-10-01 00:00:00

abstract：:Nonpiliated, phage phi 6-resistant mutants of Pseudomonas syringae pv. phaseolicola were generated by Tn5 transposon mutagenesis. A P. syringae pv. phaseolicola LR700 cosmid library was screened with Tn5-containing EcoRI fragments cloned from nonpiliated mutants. The cosmid clone pVK253 complemented the nonpiliated mu...

journal_title：Journal of bacteriology

authors： Roine E,Nunn DN,Paulin L,Romantschuk M

更新日期：1996-01-01 00:00:00

abstract：:Bacteriophage 7-7-1, a member of the family Myoviridae, infects the soil bacterium Agrobacterium sp. strain H13-3. Infection requires attachment to actively rotating bacterial flagellar filaments, with flagellar number, length, and rotation speed being important determinants for infection efficiency. To identify the s...

journal_title：Journal of bacteriology

authors： Gonzalez F,Helm RF,Broadway KM,Scharf BE

更新日期：2018-09-10 00:00:00

abstract：:In a previous study (J. O'Rear, L. Alberti, and R. M. Harshey, J. Bacteriol. 174:6125-6137, 1992) we reported the isolation of several transposon mutants of Serratia marcescens 274 that were defective either in swarming alone or in both swimming and swarming motility. All the nonflagellate (Fla-) mutants, while defect...

journal_title：Journal of bacteriology

authors： Matsuyama T,Bhasin A,Harshey RM

更新日期：1995-02-01 00:00:00

abstract：:We investigated the capacity of the hyperthermophile Pyrococcus furiosus for DNA repair by measuring survival at high levels of 60Co gamma-irradiation. The P. furiosus 2-Mb chromosome was fragmented into pieces ranging from 500 kb to shorter than 30 kb at a dose of 2,500 Gy and was fully restored upon incubation at 95...

journal_title：Journal of bacteriology

authors： DiRuggiero J,Santangelo N,Nackerdien Z,Ravel J,Robb FT

更新日期：1997-07-01 00:00:00

abstract：:Human dental biofilm communities comprise several species, which can interact cooperatively or competitively. Bacterial interactions influence biofilm formation, metabolic changes, and physiological function of the community. Lactic acid, a common metabolite of oral bacteria, was measured in the flow cell effluent of ...

journal_title：Journal of bacteriology

authors： Periasamy S,Kolenbrander PE

更新日期：2010-06-01 00:00:00

abstract：:We isolated and characterized crp mutations in Escherichia coli that allow cyclic AMP (cAMP) receptor protein to function without cAMP. These mutants defined a region involved in the cAMP-induced allosteric change of cAMP receptor protein that is necessary for activation of the protein. Currently, we have isolated int...

journal_title：Journal of bacteriology

authors： Garges S,Adhya S

更新日期：1988-04-01 00:00:00

abstract：:Twenty-four-hour-old, aerobically grown, Luria-Bertani broth cultures of Salmonella typhimurium F98 suppressed the growth of a spectinomycin-resistant (Spcr) derivative of the same strain inoculated at 10(3) CFU ml(-1). This growth suppression is genus specific and RpoS independent, and it is not solely a result of nu...

journal_title：Journal of bacteriology

authors： Zhang-Barber L,Turner AK,Martin G,Frankel G,Dougan G,Barrow PA

更新日期：1997-11-01 00:00:00

abstract：:Serratia plymuthica strain PRI-2C is a rhizosphere bacterial strain with antagonistic activity against different plant pathogens. Here we present the 5.39-Mb (G+C content, 55.67%) draft genome sequence of S. plymuthica strain PRI-2C with the aim of providing insight into the genomic basis of its antagonistic activity....

journal_title：Journal of bacteriology

authors： Garbeva P,van Elsas JD,de Boer W

更新日期：2012-08-01 00:00:00

abstract：:Pathways of ammonia assimilation into glutamic acid in Bacillus macerans were investigated by measurements of the specific activities of glutamate dehydrogenase (GDH), glutamine synthetase, and glutamate synthase. In ammonia-rich medium, GDH was the predominant pathway of ammonia assimilation. In nitrogen-fixing cells...

journal_title：Journal of bacteriology

authors： Kanamori K,Weiss RL,Roberts JD

更新日期：1987-10-01 00:00:00

abstract：:A procedure was developed for purification of ornithine transcarbamylase (OTCase) to near homogeneity from Bacillus subtilis 168. The purified native enzyme existed as a mixture of dimeric, tetrameric, and hexameric forms, but was converted to the dimer in the presence of 2-mercaptoethanol. The molecular weight of the...

journal_title：Journal of bacteriology

authors： Neway JO,Switzer RL

更新日期：1983-08-01 00:00:00

abstract：:An aryl aldehyde oxidoreductase from Nocardia sp. strain NRRL 5646 was purified 196-fold by a combination of Mono-Q, Reactive Green 19 agarose affinity, and hydroxyapatite chromatographies. The purified enzyme runs as a single band of 140 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The molecular ...

journal_title：Journal of bacteriology

authors： Li T,Rosazza JP

更新日期：1997-06-01 00:00:00

abstract：:Enterococcus faecalis is associated with a high proportion of nosocomial infections; however, little is known of the ability of this organism to proliferate in vivo. The ability of RNase B, a model glycoprotein with a single N-glycosylation site occupied by a family of high-mannose-type glycans (Man(5)- to Man(9)-GlcN...

journal_title：Journal of bacteriology

authors： Roberts G,Tarelli E,Homer KA,Philpott-Howard J,Beighton D

更新日期：2000-02-01 00:00:00

abstract：:Fertility factor F confers bacterial conjugation, a process which involves at least 20 tra genes. Resistance plasmids such as R100, R6-5, and R1 have homology with F in the tra region. Conjugal transfer of these plasmids is, however, repressed, while transfer of F is constitutive. Repression of R transfer is due to th...

journal_title：Journal of bacteriology

authors： Yoshioka Y,Ohtsubo H,Ohtsubo E

更新日期：1987-02-01 00:00:00

abstract：:Two low-molecular-weight basic proteins, termed A and B proteins, comprise about 15% of the protein of dormant spores of Bacillus megaterium. Irradiation of intact dormant spores with ultraviolet light results in covalent cross-linking of the A and B proteins to other spore macromolecules. The cross-linked A and B pro...

journal_title：Journal of bacteriology

authors： Setlow B,Setlow P

更新日期：1979-08-01 00:00:00

abstract：:Pantothenate kinase (CoaA) is a key regulator of coenzyme A (CoA) biosynthesis in Escherichia coli, and its activity is controlled by feedback inhibition by CoA and its thioesters. The importance of feedback inhibition in the control of the intracellular CoA levels was tested by constructing three site-directed mutant...

journal_title：Journal of bacteriology

authors： Rock CO,Park HW,Jackowski S

更新日期：2003-06-01 00:00:00

abstract：:The tsr gene specifies a methyl-accepting membrane protein involved in chemotaxis to serine and several repellent compounds. We have characterized a special class of tsr mutations designated cheD which alter the signaling properties of the Tsr transducer. Unlike tsr null mutants, cheD strains are generally nonchemotac...

journal_title：Journal of bacteriology

authors： Callahan AM,Parkinson JS

更新日期：1985-01-01 00:00:00

abstract：:Capnocytophaga spp. contain a group of unusual sulfonolipids, called capnoids (W. Godchaux III and E. R. Leadbetter, J. Bacteriol. 144:592-602, 1980). One of these lipids, capnine, is 2-amino-3-hydroxy-15-methylhexadecane-1-sulfonic acid; the others are, apparently, N-acylated versions of capnine. The lipids were foun...

journal_title：Journal of bacteriology

authors： Godchaux W 3rd,Leadbetter ER

更新日期：1983-03-01 00:00:00

abstract：:Induction of colicin la at a high temperature (42 C) was demonstrated in a wild strain of Shigella sonnei. After transfer of the Col factor, a similar effect was observed in those recipient cells acquiring colicinogeny. The induction is suggested to be due to the presence of a thermosensitive colicin repressor. The wi...

journal_title：Journal of bacteriology

authors： Gromkova RH

更新日期：1971-06-01 00:00:00

abstract：:Phosphopantetheine adenylyltransferase (PPAT) from Escherichia coli is an essential hexameric enzyme that catalyzes the penultimate step in coenzyme A (CoA) biosynthesis and is a target for antibacterial drug discovery. The enzyme utilizes Mg-ATP and phosphopantetheine (PhP) to generate dephospho-CoA (dPCoA) and pyrop...

journal_title：Journal of bacteriology

authors： Miller JR,Ohren J,Sarver RW,Mueller WT,de Dreu P,Case H,Thanabal V

更新日期：2007-11-01 00:00:00

abstract：:In Bacillus subtilis, the ermC gene encodes an mRNA that is unusually stable (40-min half-life) in the presence of erythromycin, an inducer of ermC gene expression. A requirement for this induced mRNA stability is a ribosome stalled in the ermC leader region. This property of ermC mRNA was used to study the decay of m...

journal_title：Journal of bacteriology

authors： Bechhofer DH,Zen KH

更新日期：1989-11-01 00:00:00

abstract：:Transposon Tn5-induced C4-dicarboxylate transport mutants of Rhizobium meliloti 2011 which could be complemented by cosmid pRmSC121 were subdivided into two classes. Class I mutants (RMS37 and RMS938) were defective in symbiotic C4-dicarboxylate transport and in nitrogen fixation. They were mutated in the structural g...

journal_title：Journal of bacteriology

authors： Engelke T,Jording D,Kapp D,Pühler A

更新日期：1989-10-01 00:00:00