Abstract:
:The protein SpoIVB plays a key role in signaling in the final sigma(K) checkpoint of Bacillus subtilis. This regulatory mechanism coordinates late gene expression during development in this organism and we have recently shown SpoIVB to be a serine peptidase. SpoIVB signals by transiting a membrane, undergoing self-cleavage, and then by an unknown mechanism activating a zinc metalloprotease, SpoIVFB, which cleaves pro-final sigma(K) to its active form, final sigma(K), in the outer mother cell chamber of the developing cell. In this work we have characterized the serine peptidase domain of SpoIVB. Alignment of SpoIVB with homologues from other spore formers has allowed site-specific mutagenesis of all potential active site residues within the peptidase domain. We have defined the putative catalytic domain of the SpoIVB serine peptidase as a 160-amino-acid residue segment at the carboxyl terminus of the protein. His236 and Ser378 are the most important residues for proteolysis, with Asp363 being the most probable third member of the catalytic triad. In addition, we have shown that mutations at residues Asn290 and His394 lead to delayed signaling in the final sigma(K) checkpoint. The active site residues suggest that SpoIVB and its homologues from other spore formers are members of a new family of serine peptidases of the trypsin superfamily.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Hoa NT,Brannigan JA,Cutting SMdoi
10.1128/jb.184.1.191-199.2002keywords:
subject
Has Abstractpub_date
2002-01-01 00:00:00pages
191-9issue
1eissn
0021-9193issn
1098-5530journal_volume
184pub_type
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doi:10.1128/JB.124.1.435-444.1975
更新日期:1975-10-01 00:00:00
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更新日期:1987-11-01 00:00:00
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更新日期:1983-10-01 00:00:00
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更新日期:1982-01-01 00:00:00
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doi:10.1128/JB.124.3.1489-1501.1975
更新日期:1975-12-01 00:00:00
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更新日期:1977-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.176.16.5077-5085.1994
更新日期:1994-08-01 00:00:00
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更新日期:1981-07-01 00:00:00
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更新日期:1991-01-01 00:00:00
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更新日期:1991-08-01 00:00:00
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更新日期:1989-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1993-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2018-04-09 00:00:00
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pub_type: 杂志文章
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更新日期:1979-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1975-12-01 00:00:00