Chromosome condensation in the absence of the non-SMC subunits of MukBEF.

Abstract:

:MukBEF is a bacterial SMC (structural maintenance of chromosome) complex required for chromosome partitioning in Escherichia coli. We report that overproduction of MukBEF results in marked chromosome condensation. This condensation is rapid and precedes the effects of overproduction on macromolecular synthesis. Condensed nucleoids are often mispositioned; however, cell viability is only mildly affected. The overproduction of MukB leads to a similar chromosome condensation, even in the absence of MukE and MukF. Thus, the non-SMC subunits of MukBEF play only an auxiliary role in chromosome condensation. MukBEF, however, was often a better condensin than MukB. Furthermore, the chromosome condensation by MukB did not rescue the temperature sensitivity of MukEF-deficient cells, nor did it suppress the high frequency of anucleate cell formation. We infer that the role of MukBEF in stabilizing chromatin architecture is more versatile than its role in controlling chromosome size. We further propose that MukBEF could be directly involved in chromosome segregation.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Wang Q,Mordukhova EA,Edwards AL,Rybenkov VV

doi

10.1128/JB.00313-06

subject

Has Abstract

pub_date

2006-06-01 00:00:00

pages

4431-41

issue

12

eissn

0021-9193

issn

1098-5530

pii

188/12/4431

journal_volume

188

pub_type

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