Expression of a mutation affecting F incompatibility in the integrated but not the autonomous state of F.

Abstract:

:Previously we have described a mutant Hfr strain in which incompatibility between the integrated F factor and an autonomous F-prime (F') factor was abolished. The mutation (inc) was located in the integrated F factor. F-prime factors isolated from the mutant Hfr strain have the same incompatibility behavior as those isolated from normal Hfr strains. Reintegration of these F' factors into the chromosome restores the Inc- phenotype characteristic of the mutant Hfr. The inc mutation thus affects incompatibility between integrated F and autonomous F(Fi-Fa incompatibility) but not incompatibility between two autonomous F factors (Fa-Fa incompatibility). The implications of this finding for the mechanism of plasmid incompatibility are discussed.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Pfister A,DeVries JK,Maas WK

doi

10.1128/JB.127.1.348-353.1976

subject

Has Abstract

pub_date

1976-07-01 00:00:00

pages

348-53

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

127

pub_type

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