Abstract:
:Neurospora crassa mutants deficient in asparagine synthetase were selected by using the procedure of inositol-less death. Complementation tests among the 100 mutants isolated suggested that their alterations were genetically allelic. Recombination analysis with strain S1007t, an asparagine auxotroph, indicated that the mutations were located near or within the asn gene on linkage group V. In vitro assays with a heterokaryon indicated that the mutation was dominant. Thermal instability of cell extracts from temperature-sensitive strains in an in vitro asparagine synthetase assay determined that the mutations were in the structural gene(s) for asparagine synthetase.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
MacPhee KG,Nelson RE,Schuster SMdoi
10.1128/JB.156.1.475-478.1983subject
Has Abstractpub_date
1983-10-01 00:00:00pages
475-8issue
1eissn
0021-9193issn
1098-5530journal_volume
156pub_type
杂志文章abstract::The DnaK protein of Zymomonas mobilis (DnaKz) was identified and found to be 80% identical to the DnaK protein of Escherichia coli on the basis of the sequence of the N-terminal 21 amino acids. The dnaKz gene was cloned and found to be expressed in a thermosensitive dnaK mutant of Escherichia coli. Expression of the f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.10.3228-3231.1993
更新日期:1993-05-01 00:00:00
abstract::Inhibition of deoxyribonucleic acid (DNA) synthesis in Escherichia coli by chemical inhibitors or by shifting cultures of temperature-sensitive elongation (dnaE and dnaB) or initiation (dnaA) mutants to nonpermissive conditions led to greatly increased synthesis of the enzyme ribonucleoside diphosphate reductase, whic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.1.107-113.1977
更新日期:1977-04-01 00:00:00
abstract:UNLABELLED:Many bacteria utilize actin-like proteins to direct peptidoglycan (PG) synthesis. MreB and MreB-like proteins are thought to act as scaffolds, guiding the localization and activity of key PG-synthesizing proteins during cell elongation. Despite their critical role in viability and cell shape maintenance, ver...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00183-16
更新日期:2016-07-13 00:00:00
abstract::Freeman, B. M. (Houghton Poultry Research Station, Huntingdon, England), and L. G. Chubb. Effect of Salmonella gallinarum on certain Krebs cycle intermediates of domestic fowl. J. Bacteriol. 88:93-95. 1964.-In chickens infected with Salmonella gallinarum, the citric acid content of the blood rose but that of the liver...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.1.93-95.1964
更新日期:1964-07-01 00:00:00
abstract::Uncoupled enzyme IIGlc of the phosphoenolpyruvate (PEP):glucose phosphotransferase system (PTS) in Salmonella typhimurium is able to catalyze glucose transport in the absence of PEP-dependent phosphorylation. As a result of the ptsG mutation, the apparent Km of the system for glucose transport is increased about 1,000...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.9.4783-4789.1990
更新日期:1990-09-01 00:00:00
abstract::Recombinant Escherichia coli strains harboring heterologous polyhydroxyalkanoate (PHA) biosynthesis genes were shown to accumulate unusually large amounts of PHA. In the present study, integrated cellular responses of metabolically engineered E. coli to the accumulation of poly(3-hydroxybutyrate) (PHB) in the early st...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.1.301-308.2001
更新日期:2001-01-01 00:00:00
abstract::Two chemotactic transducers for inorganic phosphate (P(i)), designated CtpH and CtpL, have been identified in Pseudomonas aeruginosa. The corresponding genes (ctpH and ctpL) were inactivated by inserting kanamycin and tetracycline resistance gene cassettes into the wild-type genes in the P. aeruginosa PAO1 genome. Com...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.12.3400-3404.2000
更新日期:2000-06-01 00:00:00
abstract::A fusion between lacZ and ftsZ of Escherichia coli was constructed to obtain a beta-galactosidase-FtsZ fusion protein. This fusion protein was used to raise antibodies against cell division protein FtsZ. Six monoclonal antibodies were obtained, and they reacted with FtsZ from cytoplasm and membrane fractions. The epit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.7.1886-1893.1994
更新日期:1994-04-01 00:00:00
abstract::Tn551 inactivation of femA, a factor involved in methicillin resistance of Staphylococcus aureus, caused the production of peptidoglycan in which the fraction of monoglycyl- and serine-containing muropeptides was increased at the expense of pentaglycyl muropeptides. femA mutants have a specific block in the biosynthes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.9.2779-2782.1993
更新日期:1993-05-01 00:00:00
abstract::Sinorhizobium meliloti strains lacking BacA function are impaired in symbiosis with alfalfa host plants and display altered sensitivities to a number of compounds relative to wild-type strains. With the goal of finding clues to the currently unknown biological function(s) of BacA, we carried out a genetic analysis to ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.21.6444-6453.2001
更新日期:2001-11-01 00:00:00
abstract::If bacteria are incapable of forming and incorporating proteins into the cytoplasmic membranes in all phases of the cell cycle, then not all cells from an asynchronous culture should be capable of growth when switched to a new carbon and energy source whose metabolism requires new membrane function. The transfer of an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.1.435-444.1975
更新日期:1975-10-01 00:00:00
abstract::A mutant of Rhizobium meliloti, 4R3, which is unable to grow on aspartate has been isolated. The defect is specific to aspartate utilization, since 4R3 is not an auxotroph and grows as well as its parent strain on other carbon and nitrogen sources. The defect was correlated with an inability to fix nitrogen within nod...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.9.2879-2887.1991
更新日期:1991-05-01 00:00:00
abstract::Rhodobacter sphaeroides is a free-living, photoheterotrophic bacterium known for its genomic and metabolic complexity. We have discovered that this purple photosynthetic organism possesses a quorum-sensing system. Quorum sensing occurs in a number of eukaryotic host-associated gram-negative bacteria. In these bacteria...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.23.7530-7537.1997
更新日期:1997-12-01 00:00:00
abstract::Three spontaneous derivatives of Streptococcus faecium ATCC 9790, originally isolated as conditionally Triton X-100 detergent-resistant at 25 degrees C, displayed normal penicillin-induced rates of lysis at 37 degrees C but substantially reduced rates of lysis and killing at 25 degrees C. The addition of exogenous uns...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.2.805-807.1984
更新日期:1984-08-01 00:00:00
abstract::The type III secretion (T3S) system is essential to the virulence of a large number of Gram-negative bacterial pathogens, including Yersinia. YscO is required for T3S in Yersinia and is known to interact with several other T3S proteins, including the chaperone SycD and the needle length regulator YscP. To define which...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00876-13
更新日期:2013-10-01 00:00:00
abstract::Pseudomonas aeruginosa hemolytic phospholipase C (PlcH) degrades phosphatidylcholine (PC), an abundant lipid in cell membranes and lung surfactant. A ΔplcHR mutant, known to be defective in virulence in animal models, was less able to colonize epithelial cell monolayers and was defective in biofilm formation on plasti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02169-12
更新日期:2013-07-01 00:00:00
abstract::Romano, Antonio H. (University of Cincinnati, Cincinnati, Ohio), and Dorothy J. Geason. Pattern of sheath synthesis in Sphaerotilus natans. J. Bacteriol. 88:1145-1150. 1964.-Formation of the characteristic sheath of Sphaerotilus natans was followed by immunofluorescence. Fluorescent antisheath antibody was obtained by...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.4.1145-1150.1964
更新日期:1964-10-01 00:00:00
abstract::soxR governs a superoxide response regulon that contains the genes for endonuclease IV, Mn2(+)-superoxide dismutase, and glucose 6-phosphate dehydrogenase. The soxR gene encodes a 17-kDa protein; some mutations of this gene cause constitutive overexpression of the regulon. Induction by paraquat (methyl viologen) requi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.9.2864-2871.1991
更新日期:1991-05-01 00:00:00
abstract::Bifidobacterium breve is an actinobacterium frequently isolated from colonic microbiota of breastfeeding babies. Here, we report the complete and annotated genome sequence of a B. breve strain isolated from human milk, B. breve CECT 7263. The genome sequence will provide new insights into the biology of this potential...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00691-12
更新日期:2012-07-01 00:00:00
abstract::Previously in our laboratory, a PCR-based strategy was used to isolate potential sensor gene fragments from the Staphyloccus aureus genome. One DNA fragment was isolated that shared strong sequence similarity to genes encoding bacterial sensor proteins, indicating that it originated from within a potential staphylococ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.3.611-618.1996
更新日期:1996-02-01 00:00:00
abstract::Two distinct spore appendage types of Clostridium bifermentans, a pinlike appendage and a tubular appendage, were studied by electron microscopy. The pinlike appendage is characterized by a shaft, about 100 A in diameter, which has a lobed caplike structure. The tubular appendage, 500 to 600 A in diameter, is characte...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.1.231-238.1968
更新日期:1968-01-01 00:00:00
abstract::Ferric reductase activity was examined in Azotobacter vinelandii and was found to be located in the cytoplasm. The specific activities of soluble cell extracts were not affected by the iron concentration of the growth medium; however, activity was inhibited by the presence of Zn2+ during cell growth and also by the ad...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.7.4031-4037.1989
更新日期:1989-07-01 00:00:00
abstract::The ends of the bacterial transposon gamma delta contain adjacent binding sites for gamma delta transposase and integration host factor (IHF). IHF+ and IHF- strains were used in conjunction with gamma delta transposon ends containing or lacking the site for IHF binding to determine the role that IHF plays in various g...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.9.4951-4958.1990
更新日期:1990-09-01 00:00:00
abstract::A gene in Legionella pneumophila that has significant homology to published hfq genes demonstrated regulation by RpoS and the transcriptional regulator LetA. Additionally, Hfq has a positive effect on the presence of transcripts of the genes for CsrA and the ferric uptake regulator Fur. Mutants lacking hfq demonstrate...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.4.1527-1532.2005
更新日期:2005-02-01 00:00:00
abstract::The bacterium Photorhabdus luminescens is a symbiont of the entomopathogenic nematode Heterorhabditis bacteriophora. The nematode requires the bacterium for infection of insect larvae and as a substrate for growth and reproduction. The nematodes do not grow and reproduce in insect hosts or on artificial media in the a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.10.3117-3126.2001
更新日期:2001-05-01 00:00:00
abstract::The abundance of oxidants and reductants must be balanced for an organism to thrive. Bacteria have evolved methods to prevent redox imbalances and to mitigate their deleterious consequences through the expression of detoxification enzymes, antioxidants, and systems to repair or degrade damaged proteins and DNA. Regula...
journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
doi:10.1128/JB.00128-18
更新日期:2018-08-10 00:00:00
abstract::Lactobacillus pentosus MP-10 was isolated from brines of naturally fermented Aloreña green table olives. MP-10 has potential probiotic traits, including inhibition of human pathogenic bacteria, survival at low pH (1.5), and bile salt tolerance (3%). Here, we report for the first time the annotated genome sequence of L...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05171-11
更新日期:2011-09-01 00:00:00
abstract::Lrp (leucine-responsive regulatory protein) is a global regulator of metabolism in Escherichia coli (J. M. Calvo and R. G. Matthews, Microbiol. Rev. 58:466-490, 1994). The lrp genes from three other enteric microorganisms, Enterobacter aerogenes, Klebsiella aerogenes, and Salmonella typhimurium, were cloned and sequen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.6.1624-1626.1995
更新日期:1995-03-01 00:00:00
abstract::Staphylococcus aureus responds to changing extracellular environments in part by adjusting its proteome through alterations of transcriptional priorities and selective degradation of the preexisting pool of proteins. In Bacillus subtilis, the proteolytic adaptor protein MecA has been shown to play a role in assisting ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02222-14
更新日期:2014-12-01 00:00:00
abstract::The competence-related phenotypes of mutations in each of the four open reading frames associated with the comE locus of Bacillus subtilis are described. comEA and comEC are required for transformability, whereas the products of comEB and of the overlapping comER, which is transcribed in the reverse direction, are dis...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.11.3045-3051.1995
更新日期:1995-06-01 00:00:00