Neurospora crassa mutants deficient in asparagine synthetase.

Abstract:

:Neurospora crassa mutants deficient in asparagine synthetase were selected by using the procedure of inositol-less death. Complementation tests among the 100 mutants isolated suggested that their alterations were genetically allelic. Recombination analysis with strain S1007t, an asparagine auxotroph, indicated that the mutations were located near or within the asn gene on linkage group V. In vitro assays with a heterokaryon indicated that the mutation was dominant. Thermal instability of cell extracts from temperature-sensitive strains in an in vitro asparagine synthetase assay determined that the mutations were in the structural gene(s) for asparagine synthetase.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

MacPhee KG,Nelson RE,Schuster SM

doi

10.1128/JB.156.1.475-478.1983

subject

Has Abstract

pub_date

1983-10-01 00:00:00

pages

475-8

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

156

pub_type

杂志文章
  • Cloning and expression in Escherichia coli of the dnaK gene of Zymomonas mobilis.

    abstract::The DnaK protein of Zymomonas mobilis (DnaKz) was identified and found to be 80% identical to the DnaK protein of Escherichia coli on the basis of the sequence of the N-terminal 21 amino acids. The dnaKz gene was cloned and found to be expressed in a thermosensitive dnaK mutant of Escherichia coli. Expression of the f...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.10.3228-3231.1993

    authors: Michel GP

    更新日期:1993-05-01 00:00:00

  • Regulation of ribonucleoside diphosphate reductase synthesis in Escherichia coli: increased enzyme synthesis as a result of inhibition of deoxyribonucleic acid synthesis.

    abstract::Inhibition of deoxyribonucleic acid (DNA) synthesis in Escherichia coli by chemical inhibitors or by shifting cultures of temperature-sensitive elongation (dnaE and dnaB) or initiation (dnaA) mutants to nonpermissive conditions led to greatly increased synthesis of the enzyme ribonucleoside diphosphate reductase, whic...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.1.107-113.1977

    authors: Filpula D,Fuchs JA

    更新日期:1977-04-01 00:00:00

  • YodL and YisK Possess Shape-Modifying Activities That Are Suppressed by Mutations in Bacillus subtilis mreB and mbl.

    abstract:UNLABELLED:Many bacteria utilize actin-like proteins to direct peptidoglycan (PG) synthesis. MreB and MreB-like proteins are thought to act as scaffolds, guiding the localization and activity of key PG-synthesizing proteins during cell elongation. Despite their critical role in viability and cell shape maintenance, ver...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00183-16

    authors: Duan Y,Sperber AM,Herman JK

    更新日期:2016-07-13 00:00:00

  • EFFECT OF SALMONELLA GALLINARUM ON CERTAIN KREBS CYCLE INTERMEDIATES OF DOMESTIC FOWL.

    abstract::Freeman, B. M. (Houghton Poultry Research Station, Huntingdon, England), and L. G. Chubb. Effect of Salmonella gallinarum on certain Krebs cycle intermediates of domestic fowl. J. Bacteriol. 88:93-95. 1964.-In chickens infected with Salmonella gallinarum, the citric acid content of the blood rose but that of the liver...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.1.93-95.1964

    authors: FREEMAN BM,CHUBB LG

    更新日期:1964-07-01 00:00:00

  • Adaptation of Salmonella typhimurium mutants containing uncoupled enzyme IIGlc to glucose-limited conditions.

    abstract::Uncoupled enzyme IIGlc of the phosphoenolpyruvate (PEP):glucose phosphotransferase system (PTS) in Salmonella typhimurium is able to catalyze glucose transport in the absence of PEP-dependent phosphorylation. As a result of the ptsG mutation, the apparent Km of the system for glucose transport is increased about 1,000...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.9.4783-4789.1990

    authors: Ruijter GJ,Postma PW,van Dam K

    更新日期:1990-09-01 00:00:00

  • Proteome analysis of metabolically engineered Escherichia coli producing Poly(3-hydroxybutyrate).

    abstract::Recombinant Escherichia coli strains harboring heterologous polyhydroxyalkanoate (PHA) biosynthesis genes were shown to accumulate unusually large amounts of PHA. In the present study, integrated cellular responses of metabolically engineered E. coli to the accumulation of poly(3-hydroxybutyrate) (PHB) in the early st...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.1.301-308.2001

    authors: Han MJ,Yoon SS,Lee SY

    更新日期:2001-01-01 00:00:00

  • Identification and characterization of two chemotactic transducers for inorganic phosphate in Pseudomonas aeruginosa.

    abstract::Two chemotactic transducers for inorganic phosphate (P(i)), designated CtpH and CtpL, have been identified in Pseudomonas aeruginosa. The corresponding genes (ctpH and ctpL) were inactivated by inserting kanamycin and tetracycline resistance gene cassettes into the wild-type genes in the P. aeruginosa PAO1 genome. Com...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.12.3400-3404.2000

    authors: Wu H,Kato J,Kuroda A,Ikeda T,Takiguchi N,Ohtake H

    更新日期:2000-06-01 00:00:00

  • Epitope mapping of Escherichia coli cell division protein FtsZ with monoclonal antibodies.

    abstract::A fusion between lacZ and ftsZ of Escherichia coli was constructed to obtain a beta-galactosidase-FtsZ fusion protein. This fusion protein was used to raise antibodies against cell division protein FtsZ. Six monoclonal antibodies were obtained, and they reacted with FtsZ from cytoplasm and membrane fractions. The epit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.7.1886-1893.1994

    authors: Voskuil JL,Westerbeek CA,Wu C,Kolk AH,Nanninga N

    更新日期:1994-04-01 00:00:00

  • Altered muropeptide composition in Staphylococcus aureus strains with an inactivated femA locus.

    abstract::Tn551 inactivation of femA, a factor involved in methicillin resistance of Staphylococcus aureus, caused the production of peptidoglycan in which the fraction of monoglycyl- and serine-containing muropeptides was increased at the expense of pentaglycyl muropeptides. femA mutants have a specific block in the biosynthes...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.9.2779-2782.1993

    authors: de Jonge BL,Sidow T,Chang YS,Labischinski H,Berger-Bachi B,Gage DA,Tomasz A

    更新日期:1993-05-01 00:00:00

  • Genetic analysis of the Sinorhizobium meliloti BacA protein: differential effects of mutations on phenotypes.

    abstract::Sinorhizobium meliloti strains lacking BacA function are impaired in symbiosis with alfalfa host plants and display altered sensitivities to a number of compounds relative to wild-type strains. With the goal of finding clues to the currently unknown biological function(s) of BacA, we carried out a genetic analysis to ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.21.6444-6453.2001

    authors: LeVier K,Walker GC

    更新日期:2001-11-01 00:00:00

  • Lag in adaptation to lactose as a probe to the timing of permease incorporation into the cell membrane.

    abstract::If bacteria are incapable of forming and incorporating proteins into the cytoplasmic membranes in all phases of the cell cycle, then not all cells from an asynchronous culture should be capable of growth when switched to a new carbon and energy source whose metabolism requires new membrane function. The transfer of an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.124.1.435-444.1975

    authors: Koch AL

    更新日期:1975-10-01 00:00:00

  • Aspartate aminotransferase activity is required for aspartate catabolism and symbiotic nitrogen fixation in Rhizobium meliloti.

    abstract::A mutant of Rhizobium meliloti, 4R3, which is unable to grow on aspartate has been isolated. The defect is specific to aspartate utilization, since 4R3 is not an auxotroph and grows as well as its parent strain on other carbon and nitrogen sources. The defect was correlated with an inability to fix nitrogen within nod...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.9.2879-2887.1991

    authors: Rastogi VK,Watson RJ

    更新日期:1991-05-01 00:00:00

  • A quorum-sensing system in the free-living photosynthetic bacterium Rhodobacter sphaeroides.

    abstract::Rhodobacter sphaeroides is a free-living, photoheterotrophic bacterium known for its genomic and metabolic complexity. We have discovered that this purple photosynthetic organism possesses a quorum-sensing system. Quorum sensing occurs in a number of eukaryotic host-associated gram-negative bacteria. In these bacteria...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.23.7530-7537.1997

    authors: Puskas A,Greenberg EP,Kaplan S,Schaefer AL

    更新日期:1997-12-01 00:00:00

  • Detergent-resistant Streptococcus faecium derivatives that display conditional penicillin lysis.

    abstract::Three spontaneous derivatives of Streptococcus faecium ATCC 9790, originally isolated as conditionally Triton X-100 detergent-resistant at 25 degrees C, displayed normal penicillin-induced rates of lysis at 37 degrees C but substantially reduced rates of lysis and killing at 25 degrees C. The addition of exogenous uns...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.159.2.805-807.1984

    authors: Pucci MJ,Daneo-Moore L

    更新日期:1984-08-01 00:00:00

  • Functionally essential interaction between Yersinia YscO and the T3S4 domain of YscP.

    abstract::The type III secretion (T3S) system is essential to the virulence of a large number of Gram-negative bacterial pathogens, including Yersinia. YscO is required for T3S in Yersinia and is known to interact with several other T3S proteins, including the chaperone SycD and the needle length regulator YscP. To define which...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00876-13

    authors: Mukerjea R,Ghosh P

    更新日期:2013-10-01 00:00:00

  • Anr and its activation by PlcH activity in Pseudomonas aeruginosa host colonization and virulence.

    abstract::Pseudomonas aeruginosa hemolytic phospholipase C (PlcH) degrades phosphatidylcholine (PC), an abundant lipid in cell membranes and lung surfactant. A ΔplcHR mutant, known to be defective in virulence in animal models, was less able to colonize epithelial cell monolayers and was defective in biofilm formation on plasti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02169-12

    authors: Jackson AA,Gross MJ,Daniels EF,Hampton TH,Hammond JH,Vallet-Gely I,Dove SL,Stanton BA,Hogan DA

    更新日期:2013-07-01 00:00:00

  • PATTERN OF SHEATH SYNTHESIS IN SPHAEROTILUS NATANS.

    abstract::Romano, Antonio H. (University of Cincinnati, Cincinnati, Ohio), and Dorothy J. Geason. Pattern of sheath synthesis in Sphaerotilus natans. J. Bacteriol. 88:1145-1150. 1964.-Formation of the characteristic sheath of Sphaerotilus natans was followed by immunofluorescence. Fluorescent antisheath antibody was obtained by...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.4.1145-1150.1964

    authors: ROMANO AH,GEASON DJ

    更新日期:1964-10-01 00:00:00

  • Two divergently transcribed genes, soxR and soxS, control a superoxide response regulon of Escherichia coli.

    abstract::soxR governs a superoxide response regulon that contains the genes for endonuclease IV, Mn2(+)-superoxide dismutase, and glucose 6-phosphate dehydrogenase. The soxR gene encodes a 17-kDa protein; some mutations of this gene cause constitutive overexpression of the regulon. Induction by paraquat (methyl viologen) requi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.9.2864-2871.1991

    authors: Wu J,Weiss B

    更新日期:1991-05-01 00:00:00

  • Complete genome sequence of Bifidobacterium breve CECT 7263, a strain isolated from human milk.

    abstract::Bifidobacterium breve is an actinobacterium frequently isolated from colonic microbiota of breastfeeding babies. Here, we report the complete and annotated genome sequence of a B. breve strain isolated from human milk, B. breve CECT 7263. The genome sequence will provide new insights into the biology of this potential...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00691-12

    authors: Jiménez E,Villar-Tajadura MA,Marín M,Fontecha J,Requena T,Arroyo R,Fernández L,Rodríguez JM

    更新日期:2012-07-01 00:00:00

  • Identification and molecular characterization of a putative regulatory locus that affects autolysis in Staphylococcus aureus.

    abstract::Previously in our laboratory, a PCR-based strategy was used to isolate potential sensor gene fragments from the Staphyloccus aureus genome. One DNA fragment was isolated that shared strong sequence similarity to genes encoding bacterial sensor proteins, indicating that it originated from within a potential staphylococ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.3.611-618.1996

    authors: Brunskill EW,Bayles KW

    更新日期:1996-02-01 00:00:00

  • Further electron microscope characterization of spore appendages of Clostridium bifermentans.

    abstract::Two distinct spore appendage types of Clostridium bifermentans, a pinlike appendage and a tubular appendage, were studied by electron microscopy. The pinlike appendage is characterized by a shaft, about 100 A in diameter, which has a lobed caplike structure. The tubular appendage, 500 to 600 A in diameter, is characte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.1.231-238.1968

    authors: Yolton DP,Pope L,Williams MG,Rode LJ

    更新日期:1968-01-01 00:00:00

  • Ferric reductase activity in Azotobacter vinelandii and its inhibition by Zn2+.

    abstract::Ferric reductase activity was examined in Azotobacter vinelandii and was found to be located in the cytoplasm. The specific activities of soluble cell extracts were not affected by the iron concentration of the growth medium; however, activity was inhibited by the presence of Zn2+ during cell growth and also by the ad...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.7.4031-4037.1989

    authors: Huyer M,Page WJ

    更新日期:1989-07-01 00:00:00

  • Integration host factor increases the transpositional immunity conferred by gamma delta ends.

    abstract::The ends of the bacterial transposon gamma delta contain adjacent binding sites for gamma delta transposase and integration host factor (IHF). IHF+ and IHF- strains were used in conjunction with gamma delta transposon ends containing or lacking the site for IHF binding to determine the role that IHF plays in various g...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.9.4951-4958.1990

    authors: Wiater LA,Grindley ND

    更新日期:1990-09-01 00:00:00

  • The Hfq homolog in Legionella pneumophila demonstrates regulation by LetA and RpoS and interacts with the global regulator CsrA.

    abstract::A gene in Legionella pneumophila that has significant homology to published hfq genes demonstrated regulation by RpoS and the transcriptional regulator LetA. Additionally, Hfq has a positive effect on the presence of transcripts of the genes for CsrA and the ferric uptake regulator Fur. Mutants lacking hfq demonstrate...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.4.1527-1532.2005

    authors: McNealy TL,Forsbach-Birk V,Shi C,Marre R

    更新日期:2005-02-01 00:00:00

  • A Phosphopantetheinyl transferase homolog is essential for Photorhabdus luminescens to support growth and reproduction of the entomopathogenic nematode Heterorhabditis bacteriophora.

    abstract::The bacterium Photorhabdus luminescens is a symbiont of the entomopathogenic nematode Heterorhabditis bacteriophora. The nematode requires the bacterium for infection of insect larvae and as a substrate for growth and reproduction. The nematodes do not grow and reproduce in insect hosts or on artificial media in the a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.10.3117-3126.2001

    authors: Ciche TA,Bintrim SB,Horswill AR,Ensign JC

    更新日期:2001-05-01 00:00:00

  • Reduce, Induce, Thrive: Bacterial Redox Sensing during Pathogenesis.

    abstract::The abundance of oxidants and reductants must be balanced for an organism to thrive. Bacteria have evolved methods to prevent redox imbalances and to mitigate their deleterious consequences through the expression of detoxification enzymes, antioxidants, and systems to repair or degrade damaged proteins and DNA. Regula...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,评审

    doi:10.1128/JB.00128-18

    authors: Reniere ML

    更新日期:2018-08-10 00:00:00

  • Annotated genome sequence of Lactobacillus pentosus MP-10, which has probiotic potential, from naturally fermented Aloreña green table olives.

    abstract::Lactobacillus pentosus MP-10 was isolated from brines of naturally fermented Aloreña green table olives. MP-10 has potential probiotic traits, including inhibition of human pathogenic bacteria, survival at low pH (1.5), and bile salt tolerance (3%). Here, we report for the first time the annotated genome sequence of L...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05171-11

    authors: Abriouel H,Benomar N,Pulido RP,Cañamero MM,Gálvez A

    更新日期:2011-09-01 00:00:00

  • The amino acid sequence of Lrp is highly conserved in four enteric microorganisms.

    abstract::Lrp (leucine-responsive regulatory protein) is a global regulator of metabolism in Escherichia coli (J. M. Calvo and R. G. Matthews, Microbiol. Rev. 58:466-490, 1994). The lrp genes from three other enteric microorganisms, Enterobacter aerogenes, Klebsiella aerogenes, and Salmonella typhimurium, were cloned and sequen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.6.1624-1626.1995

    authors: Friedberg D,Platko JV,Tyler B,Calvo JM

    更新日期:1995-03-01 00:00:00

  • Role of adaptor TrfA and ClpPC in controlling levels of SsrA-tagged proteins and antitoxins in Staphylococcus aureus.

    abstract::Staphylococcus aureus responds to changing extracellular environments in part by adjusting its proteome through alterations of transcriptional priorities and selective degradation of the preexisting pool of proteins. In Bacillus subtilis, the proteolytic adaptor protein MecA has been shown to play a role in assisting ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02222-14

    authors: Donegan NP,Marvin JS,Cheung AL

    更新日期:2014-12-01 00:00:00

  • ComEA, a Bacillus subtilis integral membrane protein required for genetic transformation, is needed for both DNA binding and transport.

    abstract::The competence-related phenotypes of mutations in each of the four open reading frames associated with the comE locus of Bacillus subtilis are described. comEA and comEC are required for transformability, whereas the products of comEB and of the overlapping comER, which is transcribed in the reverse direction, are dis...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.11.3045-3051.1995

    authors: Inamine GS,Dubnau D

    更新日期:1995-06-01 00:00:00