Adaptation of Salmonella typhimurium mutants containing uncoupled enzyme IIGlc to glucose-limited conditions.

Abstract:

:Uncoupled enzyme IIGlc of the phosphoenolpyruvate (PEP):glucose phosphotransferase system (PTS) in Salmonella typhimurium is able to catalyze glucose transport in the absence of PEP-dependent phosphorylation. As a result of the ptsG mutation, the apparent Km of the system for glucose transport is increased about 1,000-fold (approximately 18 mM) compared with wild-type PTS-mediated glucose transport. An S. typhimurium mutant containing uncoupled enzyme IIGlc as the sole system for glucose uptake was grown in glucose-limited chemostat cultures. Selective pressure during growth in the chemostat resulted in adaptation to the glucose-limiting conditions in two different ways. At first, mutations appeared that led to a decrease in Km value of uncoupled enzyme IIGlc. These results suggested that uncoupled enzyme IIGlc had significant control on the growth rate under glucose-limiting conditions. More efficient glucose uptake enabled a mutant to outgrow its parent and caused a decrease in the steady-state glucose concentration in the chemostat. At very low glucose concentrations (10 microM), mutants arose that contained a constitutively synthesized methyl-beta-galactoside permease. Apparently, further changes in the uncoupled enzyme IIGlc did not lead to a substantial increase in growth rate at very low glucose concentrations.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Ruijter GJ,Postma PW,van Dam K

doi

10.1128/jb.172.9.4783-4789.1990

subject

Has Abstract

pub_date

1990-09-01 00:00:00

pages

4783-9

issue

9

eissn

0021-9193

issn

1098-5530

journal_volume

172

pub_type

杂志文章
  • Molecular cloning and analysis of genes for production of K5, K7, K12, and K92 capsular polysaccharides in Escherichia coli.

    abstract::With a DNA fragment from within the region encoding the transport functions for K1 production as a hybridization probe in Southern blot experiments, homologous DNA sequences were detected in the DNA from Escherichia coli strains producing K5, K7, K92, and K100 capsular polysaccharides. No homology with the laboratory ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.168.3.1228-1233.1986

    authors: Roberts I,Mountford R,High N,Bitter-Suermann D,Jann K,Timmis K,Boulnois G

    更新日期:1986-12-01 00:00:00

  • The All-Alpha Domains of Coupling Proteins from the Agrobacterium tumefaciens VirB/VirD4 and Enterococcus faecalis pCF10-Encoded Type IV Secretion Systems Confer Specificity to Binding of Cognate DNA Substrates.

    abstract:UNLABELLED:Bacterial type IV coupling proteins (T4CPs) bind and mediate the delivery of DNA substrates through associated type IV secretion systems (T4SSs). T4CPs consist of a transmembrane domain, a conserved nucleotide-binding domain (NBD), and a sequence-variable helical bundle called the all-alpha domain (AAD). In ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00189-15

    authors: Whitaker N,Chen Y,Jakubowski SJ,Sarkar MK,Li F,Christie PJ

    更新日期:2015-07-01 00:00:00

  • The O-antigen gene cluster of Escherichia coli O55:H7 and identification of a new UDP-GlcNAc C4 epimerase gene.

    abstract::Escherichia coli O55 is an important antigen which is often associated with enteropathogenic E. coli clones. We sequenced the genes responsible for its synthesis and identified genes for O-antigen polymerase, O-antigen flippase, four enzymes involved in GDP-colitose synthesis, and three glycosyltransferases, all by co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.10.2620-2625.2002

    authors: Wang L,Huskic S,Cisterne A,Rothemund D,Reeves PR

    更新日期:2002-05-01 00:00:00

  • Purification and properties of undecyl acetate esterase from Pseudomonas cepacia grown on 2-tridecanone.

    abstract::Undecyl acetate esterase has been purified from Pseudomonas cepacia grown on the methyl ketone, 2-tridecanone. The K(m) for undecyl acetate was 2.3 x 10(-2) M. Polyacrylamide gel electrophoresis indicated that two esterase bands were being recovered during purification. These bands were separated by preparative polyac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.118.3.880-889.1974

    authors: Shum AC,Markovetz AJ

    更新日期:1974-06-01 00:00:00

  • Purification and properties of cytoplasmic and mitochondrial malate dehydrogenases of Physarum polycephalum.

    abstract::Two isoenzymes of malate dehydrogenase (MDH) were demonstrated in plasmodia of Physarum polycephalum by polyacrylamide-gel electrophoresis. The more "cathodal" form was uniquely associated with mitochondria (M-MDH) and the other form was found in the soluble cytoplasm (S-MDH). The isoenzymes were separated by acetone ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.116.2.673-684.1973

    authors: Teague WM,Henney HR Jr

    更新日期:1973-11-01 00:00:00

  • Role of the cytoplasmic membrane in the synthesis of ribonucleic acid by disrupted spheroplasts of Pseudomonas schuylkilliensis.

    abstract::Disrupted spheroplast preparations of Pseudomonas schuylkilliensis strain P contained fragments of cytoplasmic membrane and approximately 82% of the total cellular phospholipid. The protoplast-bursting factor (PB-factor), partially purified from pig pancreas, and a heat-treated pancreatic lipase fraction both inhibite...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.109.1.218-228.1972

    authors: Mizuno S,Maruo B

    更新日期:1972-01-01 00:00:00

  • Characterization of the Escherichia coli AaeAB efflux pump: a metabolic relief valve?

    abstract::Treatment of Escherichia coli with p-hydroxybenzoic acid (pHBA) resulted in upregulation of yhcP, encoding a protein of the putative efflux protein family. Also upregulated were the adjacent genes yhcQ, encoding a protein of the membrane fusion protein family, and yhcR, encoding a small protein without a known or sugg...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.21.7196-7204.2004

    authors: Van Dyk TK,Templeton LJ,Cantera KA,Sharpe PL,Sariaslani FS

    更新日期:2004-11-01 00:00:00

  • The cell-bound fructosyltransferase of Streptococcus salivarius: the carboxyl terminus specifies attachment in a Streptococcus gordonii model system.

    abstract::The ftf gene, coding for the cell-bound beta-D-fructosyltransferase (FTF) of Streptococcus salivarius ATCC 25975, has been analyzed, and its deduced amino acid sequence has been compared with that of the secreted FTF of Streptococcus mutans and the levansucrases (SacBs) of Bacillus species. A unique proline-rich regio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.14.4520-4527.1993

    authors: Rathsam C,Giffard PM,Jacques NA

    更新日期:1993-07-01 00:00:00

  • Phosphate starvation regulon of Salmonella typhimurium.

    abstract::Several phosphate-starvation-inducible (psi) genetic loci in Salmonella typhimurium were identified by fusing the lacZ gene to psi promoters by using the Mu d1 and Mu d1-8 bacteriophages. Although several different starvation conditions were examined, the psi loci responded solely to phosphate deprivation. A regulator...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.166.2.666-669.1986

    authors: Foster JW,Spector MP

    更新日期:1986-05-01 00:00:00

  • Substrate recognition properties of oligopeptidase B from Salmonella enterica serovar Typhimurium.

    abstract::Oligopeptidase B (OpdB) is a serine peptidase broadly distributed among unicellular eukaryotes, gram-negative bacteria, and spirochetes which has emerged as an important virulence factor and potential therapeutic target in infectious diseases. We report here the cloning and expression of the opdB homologue from Salmon...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.12.3329-3337.2002

    authors: Morty RE,Fülöp V,Andrews NW

    更新日期:2002-06-01 00:00:00

  • Microtubular structures in a stable staphylococcal L-form.

    abstract::Microtubular structures, which were demonstrated as straight, dense-walled cylinders attached to cell membranes, were found in a stable staphylococcal L-form grown in the absence of antibiotic. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.132.3.1024-1026.1977

    authors: Eda T,Kanda Y,Mori C,Kimura S

    更新日期:1977-12-01 00:00:00

  • Characterization of a pseudomonad 2-nitrobenzoate nitroreductase and its catabolic pathway-associated 2-hydroxylaminobenzoate mutase and a chemoreceptor involved in 2-nitrobenzoate chemotaxis.

    abstract::Pseudomonas fluorescens strain KU-7 is a prototype microorganism that metabolizes 2-nitrobenzoate (2-NBA) via the formation of 3-hydroxyanthranilate (3-HAA), a known antioxidant and reductant. The initial two steps leading to the sequential formation of 2-hydroxy/aminobenzoate and 3-HAA are catalyzed by a NADPH-depend...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01098-06

    authors: Iwaki H,Muraki T,Ishihara S,Hasegawa Y,Rankin KN,Sulea T,Boyd J,Lau PC

    更新日期:2007-05-01 00:00:00

  • Changes in deoxyribonucleic acid polymerase activities in synthesis of deoxyribonucleic acid during sporulation of Bacillus subtilis.

    abstract::The deoxyribonucleic acid (DNA) polymerase activities in Bacillus subtilis strains Marburg 168 (thy-trp2) and D22, a DNA polymerase I-deficient mutant, were measured at various stages of sporulation. The DNA polymerase I activity, which had decreased after the exponential growth, began to increase at the early stage o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.128.1.221-227.1976

    authors: Honjo M,Shibano Y,Komano T

    更新日期:1976-10-01 00:00:00

  • Mutagenic DNA repair in enterobacteria.

    abstract::Sixteen species of enterobacteria have been screened for mutagenic DNA repair activity. In Escherichia coli, mutagenic DNA repair is encoded by the umuDC operon. Synthesis of UmuD and UmuC proteins is induced as part of the SOS response to DNA damage, and after induction, the UmuD protein undergoes an autocatalytic cl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.18.5604-5611.1991

    authors: Sedgwick SG,Ho C,Woodgate R

    更新日期:1991-09-01 00:00:00

  • Genome sequencing of a genetically tractable Pyrococcus furiosus strain reveals a highly dynamic genome.

    abstract::The model archaeon Pyrococcus furiosus grows optimally near 100°C on carbohydrates and peptides. Its genome sequence (NCBI) was determined 12 years ago. A genetically tractable strain, COM1, was very recently reported, and here we describe its genome sequence. Of 1,909,827 bp in size, it is 1,571 bp longer (0.1%) than...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00439-12

    authors: Bridger SL,Lancaster WA,Poole FL 2nd,Schut GJ,Adams MW

    更新日期:2012-08-01 00:00:00

  • Levels of the secreted Vibrio cholerae attachment factor GbpA are modulated by quorum-sensing-induced proteolysis.

    abstract::Vibrio cholerae is the etiologic agent of cholera in humans. Intestinal colonization occurs in a stepwise fashion, initiating with attachment to the small intestinal epithelium. This attachment is followed by expression of the toxin-coregulated pilus, microcolony formation, and cholera toxin (CT) production. We have r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00747-09

    authors: Jude BA,Martinez RM,Skorupski K,Taylor RK

    更新日期:2009-11-01 00:00:00

  • Nutritional requirements for vegetative growth of Myxococcus xanthus.

    abstract::Dworkin, Martin (Indiana University Medical Center, Indianapolis, Ind.). Nutritional requirements for vegetative growth of Myxococcus xanthus. J. Bacteriol. 84:250-257. 1962.-This investigation was part of a program to clarify the environmental regulation of fruiting-body formation in a fruiting myxobacterium. By use ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.84.2.250-257.1962

    authors: DWORKIN M

    更新日期:1962-08-01 00:00:00

  • Expression of a functional fungal polyketide synthase in the bacterium Streptomyces coelicolor A3(2).

    abstract::The multifunctional 6-methylsalicylic acid synthase gene from Penicillium patulum was engineered for regulated expression in Streptomyces coelicolor. Production of significant amounts of 6-methylsalicylic acid by the recombinant strain was proven by nuclear magnetic resonance spectroscopy. These results suggest that i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.15.4544-4548.1995

    authors: Bedford DJ,Schweizer E,Hopwood DA,Khosla C

    更新日期:1995-08-01 00:00:00

  • Determination of transcriptional units and gene products from the ftsA region of Escherichia coli.

    abstract::Lambda transducing phage gamma 16-2 carries the genes envA, ftsZ, ftsA, ddl, and murC and directs the synthesis of six unique proteins in ultraviolet-irradiated cells. Various derivatives of gamma 16-2 carrying smaller segments of the bacterial deoxyribonucleic acid have also been analyzed for their capacity to direct...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.143.3.1281-1288.1980

    authors: Lutkenhaus JF,Wu HC

    更新日期:1980-09-01 00:00:00

  • Roles of DegP in prevention of protein misfolding in the periplasm upon overexpression of penicillin acylase in Escherichia coli.

    abstract::Enhancement of the production of soluble recombinant penicillin acylase in Escherichia coli via coexpression of a periplasmic protease/chaperone, DegP, was demonstrated. Coexpression of DegP resulted in a shift of in vivo penicillin acylase (PAC) synthesis flux from the nonproductive pathway to the productive one when...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.10.3020-3030.2003

    authors: Pan KL,Hsiao HC,Weng CL,Wu MS,Chou CP

    更新日期:2003-05-01 00:00:00

  • Carbon dioxide fixation by cells of Streptococcus faecalis var. liquefaciens.

    abstract::Fixation of NaH(14)CO(3) by a heavy cell suspension of Streptococcus faecalis var. liquefaciens was studied. Several nutrients, pyridoxal, riboflavine, adenine, uracil, and O(2) stimulated (14)CO(2) incorporation into cells only under conditions that were adequate for synthesis of cell macromolecules. Biotin increased...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.1.27-33.1970

    authors: Goff RC,Hartman RE

    更新日期:1970-10-01 00:00:00

  • Inhibition of arginyl-transfer ribonucleic acid synthetase activity of Escherichia coli by arginine biosynthetic precursors.

    abstract::The arginine biosynthetic precursors, ornithine, citrulline, and argininosuccinate, inhibit arginyl-transfer ribonucleic acid (tRNA) synthetase (EC 6.1.1.13, arginine: soluble RNA ligase, adenosine monophosphate) activity in the in vitro attachment assay system. Ornithine is the most potent, argininosuccinate is next,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.107.2.589-591.1971

    authors: Yem DW,Williams LS

    更新日期:1971-08-01 00:00:00

  • Symbiotic autoregulation of nifA expression in Rhizobium leguminosarum bv. viciae.

    abstract::NifA is the general transcriptional activator of nitrogen fixation genes in diazotrophic bacteria. In Rhizobium leguminosarum bv. viciae UPM791, the nifA gene is part of a gene cluster (orf71 orf79 fixW orf5 fixABCX nifAB) separated by 896 bp from an upstream and divergent truncated duplication of nifH (DeltanifH). Sy...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.19.6586-6594.2004

    authors: Martínez M,Palacios JM,Imperial J,Ruiz-Argüeso T

    更新日期:2004-10-01 00:00:00

  • Novel one-step cloning vector with a transposable element: application to the Myxococcus xanthus genome.

    abstract::A new strategy was developed for rapid cloning of genes with a transposon mutation library. We constructed a transposon designated TnV that was derived from Tn5 and consists of the gene coding for neomycin phosphotransferase II as well as the replication origin of an Escherichia coli plasmid, pSC101, flanked by Tn5 in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.1.270-275.1985

    authors: Furuichi T,Inouye M,Inouye S

    更新日期:1985-10-01 00:00:00

  • Genome diversification in phylogenetic lineages I and II of Listeria monocytogenes: identification of segments unique to lineage II populations.

    abstract::Thirteen different serotypes of Listeria monocytogenes can be distinguished on the basis of variation in somatic and flagellar antigens. Although the known virulence genes are present in all serotypes, greater than 90% of human cases of listeriosis are caused by serotypes 1/2a, 1/2b, and 4b and nearly all outbreaks of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.18.5573-5584.2003

    authors: Zhang C,Zhang M,Ju J,Nietfeldt J,Wise J,Terry PM,Olson M,Kachman SD,Wiedmann M,Samadpour M,Benson AK

    更新日期:2003-09-01 00:00:00

  • Regulation by a novel protein of the bimodal distribution of lipopolysaccharide in the outer membrane of Escherichia coli.

    abstract::We report on the cloning and characterization of the rfb gene cluster of the O75 lipopolysaccharide from a urinary tract isolate of Escherichia coli. Deletion cloning defined the minimum region of DNA that expressed the O75 antigen in E. coli host strains to be on a 12.4-kb insert. However, the E. coli strain expressi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.18.5699-5704.1991

    authors: Batchelor RA,Haraguchi GE,Hull RA,Hull SI

    更新日期:1991-09-01 00:00:00

  • EXPLANATION FOR LIMITATION OF POPULATIONS OF ESCHERICHIA COLI IN BROTH CULTURES.

    abstract::Freter, Rolf (Jefferson Medical College, Philadelphia, Pa.), and Atsushi Ozawa. Explanation for limitation of populations of Escherichia coli in broth cultures. J. Bacteriol. 86:904-910. 1963.-Veal Infusion Broth surrounding a cellophane bag containing a 24-hr culture of Escherichia coli was inhibitory to an inoculum ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.86.5.904-910.1963

    authors: FRETER R,OZAWA A

    更新日期:1963-11-01 00:00:00

  • Cloning, sequencing, and expression in Escherichia coli of the gene encoding a 45-kilodalton protein, elongation factor Tu, from Chlamydia trachomatis serovar F.

    abstract::The gene encoding a 45-kDa protein (45K) of Chlamydia trachomatis serovar F was cloned, sequenced, and overexpressed in Escherichia coli. Alignment of the deduced peptide sequence with E. coli elongation factor Tu (EF-Tu) demonstrated 69% identity. The 45K was recognized by a Chlamydia genus-specific monoclonal antibo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.4.1184-1187.1994

    authors: Zhang YX,Shi Y,Zhou M,Petsko GA

    更新日期:1994-02-01 00:00:00

  • Cytochemical localization of catalase activity in yeast peroxisomes.

    abstract::Diaminobenzidine oxidation product occurred in peroxisomes and in the intracristate spaces of mitochondria. The reaction was inhibited only in peroxisomes when 3-amino-1,2,4-triazole was present, but cyanide and azide inhibited deposition in both kinds of organelles. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.1.581-584.1970

    authors: Hoffmann HP,Szabo A,Avers CJ

    更新日期:1970-10-01 00:00:00

  • Pleiotropic and epistatic behavior of a ring-hydroxylating oxygenase system in the polycyclic aromatic hydrocarbon metabolic network from Mycobacterium vanbaalenii PYR-1.

    abstract::Despite the considerable knowledge of bacterial high-molecular-weight (HMW) polycyclic aromatic hydrocarbon (PAH) metabolism, the key enzyme(s) and its pleiotropic and epistatic behavior(s) responsible for low-molecular-weight (LMW) PAHs in HMW PAH-metabolic networks remain poorly understood. In this study, a phenotyp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01945-14

    authors: Kweon O,Kim SJ,Kim DW,Kim JM,Kim HL,Ahn Y,Sutherland JB,Cerniglia CE

    更新日期:2014-10-01 00:00:00