Abstract:
:Uncoupled enzyme IIGlc of the phosphoenolpyruvate (PEP):glucose phosphotransferase system (PTS) in Salmonella typhimurium is able to catalyze glucose transport in the absence of PEP-dependent phosphorylation. As a result of the ptsG mutation, the apparent Km of the system for glucose transport is increased about 1,000-fold (approximately 18 mM) compared with wild-type PTS-mediated glucose transport. An S. typhimurium mutant containing uncoupled enzyme IIGlc as the sole system for glucose uptake was grown in glucose-limited chemostat cultures. Selective pressure during growth in the chemostat resulted in adaptation to the glucose-limiting conditions in two different ways. At first, mutations appeared that led to a decrease in Km value of uncoupled enzyme IIGlc. These results suggested that uncoupled enzyme IIGlc had significant control on the growth rate under glucose-limiting conditions. More efficient glucose uptake enabled a mutant to outgrow its parent and caused a decrease in the steady-state glucose concentration in the chemostat. At very low glucose concentrations (10 microM), mutants arose that contained a constitutively synthesized methyl-beta-galactoside permease. Apparently, further changes in the uncoupled enzyme IIGlc did not lead to a substantial increase in growth rate at very low glucose concentrations.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Ruijter GJ,Postma PW,van Dam Kdoi
10.1128/jb.172.9.4783-4789.1990subject
Has Abstractpub_date
1990-09-01 00:00:00pages
4783-9issue
9eissn
0021-9193issn
1098-5530journal_volume
172pub_type
杂志文章abstract::With a DNA fragment from within the region encoding the transport functions for K1 production as a hybridization probe in Southern blot experiments, homologous DNA sequences were detected in the DNA from Escherichia coli strains producing K5, K7, K92, and K100 capsular polysaccharides. No homology with the laboratory ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.3.1228-1233.1986
更新日期:1986-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.118.3.880-889.1974
更新日期:1974-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.116.2.673-684.1973
更新日期:1973-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.109.1.218-228.1972
更新日期:1972-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.14.4520-4527.1993
更新日期:1993-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.166.2.666-669.1986
更新日期:1986-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.132.3.1024-1026.1977
更新日期:1977-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2007-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.128.1.221-227.1976
更新日期:1976-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.18.5604-5611.1991
更新日期:1991-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2012-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00747-09
更新日期:2009-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.2.250-257.1962
更新日期:1962-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1980-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1970-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1971-08-01 00:00:00
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更新日期:2004-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1985-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1991-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.5.904-910.1963
更新日期:1963-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1994-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1970-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-10-01 00:00:00