Abstract:
:Pseudomonas fluorescens strain KU-7 is a prototype microorganism that metabolizes 2-nitrobenzoate (2-NBA) via the formation of 3-hydroxyanthranilate (3-HAA), a known antioxidant and reductant. The initial two steps leading to the sequential formation of 2-hydroxy/aminobenzoate and 3-HAA are catalyzed by a NADPH-dependent 2-NBA nitroreductase (NbaA) and 2-hydroxylaminobenzoate mutase (NbaB), respectively. The 216-amino-acid protein NbaA is 78% identical to a plasmid-encoded hypothetical conserved protein of Polaromonas strain JS666; structurally, it belongs to the homodimeric NADH:flavin mononucleotide (FMN) oxidoreductase-like fold family. Structural modeling of complexes with the flavin, coenzyme, and substrate suggested specific residues contributing to the NbaA catalytic activity, assuming a ping-pong reaction mechanism. Mutational analysis supports the roles of Asn40, Asp76, and Glu113, which are predicted to form the binding site for a divalent metal ion implicated in FMN binding, and a role in NADPH binding for the 10-residue insertion in the beta5-alpha2 loop. The 181-amino-acid sequence of NbaB is 35% identical to the 4-hydroxylaminobenzoate lyases (PnbBs) of various 4-nitrobenzoate-assimilating bacteria, e.g., Pseudomonas putida strain TW3. Coexpression of nbaB with nbaA in Escherichia coli produced a small amount of 3-HAA from 2-NBA, supporting the functionality of the nbaB gene. We also showed by gene knockout and chemotaxis assays that nbaY, a chemoreceptor NahY homolog located downstream of the nbaA gene, is responsible for strain KU-7 being attracted to 2-NBA. NbaY is the first chemoreceptor in nitroaromatic metabolism to be identified, and this study completes the gene elucidation of 2-NBA metabolism that is localized within a 24-kb chromosomal locus of strain KU-7.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Iwaki H,Muraki T,Ishihara S,Hasegawa Y,Rankin KN,Sulea T,Boyd J,Lau PCdoi
10.1128/JB.01098-06subject
Has Abstractpub_date
2007-05-01 00:00:00pages
3502-14issue
9eissn
0021-9193issn
1098-5530pii
JB.01098-06journal_volume
189pub_type
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doi:10.1128/JB.123.3.878-887.1975
更新日期:1975-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.9.5064-5070.1990
更新日期:1990-09-01 00:00:00
abstract::Sinorhizobium meliloti is a soil-dwelling alphaproteobacterium that engages in a nitrogen-fixing root nodule symbiosis with leguminous plants. Cell surface polysaccharides are important both for adapting to stresses in the soil and for the development of an effective symbiotic interaction. Among the polysaccharides ch...
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journal_title:Journal of bacteriology
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doi:10.1128/JB.87.5.1003-1010.1964
更新日期:1964-05-01 00:00:00
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
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doi:10.1128/JB.110.1.246-255.1972
更新日期:1972-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1969-03-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1985-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-03-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.1.226-232.1964
更新日期:1964-07-01 00:00:00
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更新日期:1964-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1994-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.11.3656-3660.1993
更新日期:1993-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.171.1.360-368.1989
更新日期:1989-01-01 00:00:00
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更新日期:2011-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2010-06-01 00:00:00
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更新日期:2000-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1967-05-01 00:00:00