Purification and characterization of acid phosphatase V from Aspergillus nidulans.

abstract：:We have cloned and sequenced a 505-base-pair (bp) segment of DNA situated upstream of mvaA, the structural gene for (S)-3-hydroxy-3-methylglutaryl coenzyme A reductase (EC 1.1.1.88) of Pseudomonas mevalonii. The DNA segment that we characterized includes the promoter region for the mva operon. Nuclease S1 mapping and ...

journal_title：Journal of bacteriology

authors： Wang YL,Beach MJ,Rodwell VW

更新日期：1989-10-01 00:00:00

abstract：:Exoelectrogens are able to transfer electrons extracellularly, enabling them to respire on insoluble terminal electron acceptors. Extensively studied exoelectrogens, such as Geobacter sulfurreducens and Shewanella oneidensis, are Gram negative. More recently, it has been reported that Gram-positive bacteria, such as L...

journal_title：Journal of bacteriology

authors： Jeuken LJC,Hards K,Nakatani Y

更新日期：2020-03-11 00:00:00

abstract：:Microcin J25 (MccJ25) is a small peptide antibiotic produced by an Escherichia coli strain isolated from human feces. The genetic determinants for MccJ25 synthesis and immunity have been cloned from the low-copy-number wild-type plasmid pTUC1OO into the compatible vectors pBR322 and pACYC184. Physical and phenotypical...

journal_title：Journal of bacteriology

authors： Solbiati JO,Ciaccio M,Farías RN,Salomón RA

更新日期：1996-06-01 00:00:00

abstract：:The major outer membrane lipoprotein (Lpp) of Escherichia coli possesses serine at position 2, which is thought to function as the outer membrane sorting signal, and lysine at the C terminus, through which Lpp covalently associates with peptidoglycan. Arginine (R) is present before the C-terminal lysine in the wild-ty...

journal_title：Journal of bacteriology

authors： Yakushi T,Tajima T,Matsuyama S,Tokuda H

更新日期：1997-05-01 00:00:00

abstract：:Bergdoll, Merlin S. (University of Chicago, Chicago, Ill.), Concordia R. Borja, and Remedios M. Avena. Identification of a new enterotoxin as enterotoxin C. J. Bacteriol. 90:1481-1485. 1965.-Identification of a new enterotoxin was accomplished by purification of the enterotoxins produced by staphylococcal strains 137 ...

journal_title：Journal of bacteriology

authors： Bergdoll MS,Borja CR,Avena RM

更新日期：1965-11-01 00:00:00

abstract：:Sixteen species of enterobacteria have been screened for mutagenic DNA repair activity. In Escherichia coli, mutagenic DNA repair is encoded by the umuDC operon. Synthesis of UmuD and UmuC proteins is induced as part of the SOS response to DNA damage, and after induction, the UmuD protein undergoes an autocatalytic cl...

journal_title：Journal of bacteriology

authors： Sedgwick SG,Ho C,Woodgate R

更新日期：1991-09-01 00:00:00

abstract：:We cloned the cryptic phn operon of a K-12 strain, phn(EcoK), and analyzed the nucleotide sequence of the phn region (11,672 bp). An mRNA start site upstream of the phnC gene was identified by S1 nuclease mapping. The pho regulon activator PhoB protects a pho box region near the mRNA start in DNase I footprinting and ...

journal_title：Journal of bacteriology

authors： Makino K,Kim SK,Shinagawa H,Amemura M,Nakata A

更新日期：1991-04-01 00:00:00

abstract：:In the course of study on ribonucleic acid (RNA) metabolism during sporulation in Saccharomyces cerevisiae, a new species of RNA (20S) was observed in sporulating cells by polyacrylamide gel electrophoresis. The relative content of this RNA to total RNA increased linearly early in sporulation. Labeled adenine was pref...

journal_title：Journal of bacteriology

authors： Kadowaki K,Halvorson HO

更新日期：1971-03-01 00:00:00

abstract：:The nucleotide second messengers pppGpp and ppGpp [(p)ppGpp] are responsible for the global downregulation of transcription, translation, DNA replication, and growth rate that occurs during the stringent response. More recent studies suggest that (p)ppGpp is also an important effector in many nonstringent processes, i...

journal_title：Journal of bacteriology

authors： Ababneh QO,Herman JK

更新日期：2015-01-01 00:00:00

abstract：:To isolate strains with new recA mutations that differentially affect RecA protein functions, we mutagenized in vitro the recA gene carried by plasmid mini-F and then introduced the mini-F-recA plasmid into a delta recA host that was lysogenic for prophage phi 80 and carried a lac duplication. By scoring prophage indu...

journal_title：Journal of bacteriology

authors： Dutreix M,Moreau PL,Bailone A,Galibert F,Battista JR,Walker GC,Devoret R

更新日期：1989-05-01 00:00:00

abstract：:The steps in the biosynthetic transformation of GTP to 7,8-dihydro-D-erythro-neopterin (H2neopterin), the precursor to the modified folates found in the methanogenic archaea, has been elucidated for the first time in two members of the domain Archaea. In Methanococcus thermophila and Methanobacterium thermoautotrophic...

journal_title：Journal of bacteriology

authors： Howell DM,White RH

更新日期：1997-08-01 00:00:00

abstract：:The interaction of 12 phage strains with bacilli, protoplasts, and L forms of Bacillus subtilis 168 and with eight of its mutants and two of its lysogens is described qualitatively and quantitatively. After removal of the cell wall from B. subtilis 168, 11 of the 12 phage strains can still adsorb to the protoplasts, n...

journal_title：Journal of bacteriology

authors： Jacobson ED,Landman OE

更新日期：1975-10-01 00:00:00

abstract：:A specific gamma-aminobutyrate (GABA) transport system in Escherichia coli K-12 cells with a K(m) of 12 muM and a V(max) of 278 nmol/ml of intracellular water per min is described. Membrane vesicles contained d-lactate-dependent activity of the system. Mutants defective in GABA transport were isolated; they lost the a...

journal_title：Journal of bacteriology

authors： Kahane S,Levitz R,Halpern YS

更新日期：1978-08-01 00:00:00

abstract：:The antiadaptor protein IraD inhibits the proteolysis of the alternative sigma factor, RpoS, which promotes the synthesis of >100 genes during the general stress response and during stationary phase. Our previous results showed that IraD determines RpoS steady-state levels during exponential growth and mediates its st...

journal_title：Journal of bacteriology

authors： Merrikh H,Ferrazzoli AE,Lovett ST

更新日期：2009-12-01 00:00:00

abstract：:Tests for utilization of d-octopine and nopaline in defined media containing a carbon and nitrogen source were made on 60 strains of Agrobacterium representing four species and on a representative of each of five species of Rhizobium. Among 46 virulent strains of Agrobacterium, only two strains were found which utiliz...

journal_title：Journal of bacteriology

authors： Lippincott JA,Beiderbeck R,Lippincott BB

更新日期：1973-10-01 00:00:00

abstract：:Nisin is a cationic antimicrobial peptide that belongs to the group of lantibiotics. It is thought to form oligomeric pores in the target membrane by a mechanism that requires the transmembrane electrical potential delta psi and that involves local pertubation of the lipid bilayer structure. Here we show that nisin do...

journal_title：Journal of bacteriology

authors： Moll GN,Clark J,Chan WC,Bycroft BW,Roberts GC,Konings WN,Driessen AJ

更新日期：1997-01-01 00:00:00

abstract：:We have developed a method, using laser, optical tweezers and direct microscopic analysis of reproductive potential and membrane integrity, to assess single-cell viability in a stationary-phase Escherichia coli population. It is demonstrated here that a reduction in cell integrity, determined by using the fluorescent ...

journal_title：Journal of bacteriology

authors： Ericsson M,Hanstorp D,Hagberg P,Enger J,Nyström T

更新日期：2000-10-01 00:00:00

abstract：:Burchard, Robert P. (University of Minnesota, Minneapolis), and Martin Dworkin. Light-induced lysis and carotenogenesis in Myxococcus xanthus. J. Bacteriol. 91:535-545. 1966.-Myxococcus xanthus, grown vegetatively in the light, developed an orange carotenoid after the cells entered stationary phase of growth; pigment ...

journal_title：Journal of bacteriology

authors： Burchard RP,Dworkin M

更新日期：1966-02-01 00:00:00

abstract：:Anabaena variabilis ATCC 29413 is a heterotrophic, nitrogen-fixing cyanobacterium that has been reported to fix nitrogen and reduce acetylene to ethane in the absence of molybdenum. DNA from this strain hybridized well at low stringency to the nitrogenase 2 (vnfDGK) genes of Azotobacter vinelandii. The hybridizing reg...

journal_title：Journal of bacteriology

authors： Thiel T

更新日期：1993-10-01 00:00:00

abstract：:RecAX53 is a chimeric variant of the Escherichia coli RecA protein (RecAEc) that contains a part of the central domain of Pseudomonas aeruginosa RecA (RecAPa), encompassing a region that differs from RecAEc at 12 amino acid positions. Like RecAPa, this chimera exhibits hyperrecombination activity in E. coli cells, inc...

journal_title：Journal of bacteriology

authors： Baitin DM,Bakhlanova IV,Chervyakova DV,Kil YV,Lanzov VA,Cox MM

更新日期：2008-04-01 00:00:00

abstract：:Faithful DNA replication involves the removal of RNA residues from genomic DNA prior to the ligation of nascent DNA fragments in all living organisms. Because the physiological roles of archaeal type 2 RNase H are not fully understood, the substrate structure requirements for the detection of RNase H activity need fur...

journal_title：Journal of bacteriology

authors： Le Laz S,Le Goaziou A,Henneke G

更新日期：2010-07-01 00:00:00

abstract：UNLABELLED:Rhodobacter sphaeroides is a free-living alphaproteobacterium that contains two clusters of functional flagellar genes in its genome: one acquired by horizontal gene transfer (fla1) and one that is endogenous (fla2). We have shown that the Fla2 system is normally quiescent and under certain conditions produc...

journal_title：Journal of bacteriology

authors： de la Mora J,Uchida K,del Campo AM,Camarena L,Aizawa S,Dreyfus G

更新日期：2015-09-01 00:00:00

abstract：:Numerous field isolates of Moraxella bovis have previously been classified by serological techniques into seven serogroups, each defined by homologous cross-reaction with antisera prepared against purified pili of a single prototype strain. The gene encoding pilin from each of the prototype strains has been characteri...

journal_title：Journal of bacteriology

authors： Atwell JL,Tennent JM,Lepper AW,Elleman TC

更新日期：1994-08-01 00:00:00

abstract：:Single-celled organisms must survive exposure to environmental extremes. Perhaps one of the most variable and potentially life-threatening changes that can occur is that of a rapid and acute decrease in external osmolarity. This easily translates into several atmospheres of additional pressure that can build up within...

journal_title：Journal of bacteriology

authors： Booth IR,Blount P

更新日期：2012-09-01 00:00:00

abstract：:The Tat system, found in the cytoplasmic membrane of many bacteria, is a general export pathway for folded proteins. Here we describe the development of a method, based on the transport of chloramphenicol acetyltransferase, that allows positive selection of mutants defective in Tat function. We have demonstrated the u...

journal_title：Journal of bacteriology

authors： Hicks MG,Lee PA,Georgiou G,Berks BC,Palmer T

更新日期：2005-04-01 00:00:00

abstract：:Pseudomonas aeruginosa in the lungs of cystic fibrosis (CF) patients is characterized by a series of genotypic and phenotypic changes that reflect the transition from acute to chronic infection. These include the overproduction of the exopolysaccharide alginate and the loss of complete lipopolysaccharide (LPS). LPS is...

journal_title：Journal of bacteriology

authors： Davis MR Jr,Muszynski A,Lollett IV,Pritchett CL,Carlson RW,Goldberg JB

更新日期：2013-04-01 00:00:00

abstract：:Borrelia burgdorferi is a motile spirochete which has been identified as the causative microorganism in Lyme disease. The physiological functions which govern the motility of this organism have not been elucidated. In this study, we found that motility of B. burgdorferi required an environment similar to interstitial ...

journal_title：Journal of bacteriology

authors： Shi W,Yang Z,Geng Y,Wolinsky LE,Lovett MA

更新日期：1998-01-01 00:00:00

abstract：:The ability of Clostridium perfringens type A to produce an enterotoxin active in human food poisoning has been shown to be directly related to the ability of the organism to sporulate. Enterotoxin was produced only in a sporulation medium and not in a growth medium in which sporulation was repressed. Mutants with an ...

journal_title：Journal of bacteriology

authors： Duncan CL,Strong DH,Sebald M

更新日期：1972-04-01 00:00:00

abstract：:During Bacillus subtilis sporulation, the transient engulfment defect of spoIIB strains is enhanced by spoVG null mutations and suppressed by spoVS null mutations. These mutations have opposite effects on expression of the motility regulon, as the spoVG mutation reduces and the spoVS mutation increases sigmaD-directed...

journal_title：Journal of bacteriology

authors： Perez AR,Abanes-De Mello A,Pogliano K

更新日期：2006-02-01 00:00:00

abstract：:The potassium permeability of Rickettsia prowazekii was characterized by chemical measurement of the intracellular sodium and potassium pools and isotopic flux measurements with 86Rb+ as a tracer. R. prowazekii, in contrast to Escherichia coli, did not maintain a high potassium-to-sodium ratio in their cytoplasm excep...

journal_title：Journal of bacteriology

authors： Winkler HH

更新日期：1984-01-01 00:00:00