Abstract:
:Faithful DNA replication involves the removal of RNA residues from genomic DNA prior to the ligation of nascent DNA fragments in all living organisms. Because the physiological roles of archaeal type 2 RNase H are not fully understood, the substrate structure requirements for the detection of RNase H activity need further clarification. Biochemical characterization of a single RNase H detected within the genome of Pyrococcus abyssi showed that this type 2 RNase H is an Mg- and alkaline pH-dependent enzyme. PabRNase HII showed RNase activity and acted as a specific endonuclease on RNA-DNA/DNA duplexes. This specific cleavage, 1 nucleotide upstream of the RNA-DNA junction, occurred on a substrate in which RNA initiators had to be fully annealed to the cDNA template. On the other hand, a 5' RNA flap Okazaki fragment intermediate impaired PabRNase HII endonuclease activity. Furthermore, introduction of mismatches into the RNA portion near the RNA-DNA junction decreased both the specificity and the efficiency of cleavage by PabRNase HII. Additionally, PabRNase HII could cleave a single ribonucleotide embedded in a double-stranded DNA. Our data revealed PabRNase HII as a dual-function enzyme likely required for the completion of DNA replication and DNA repair.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Le Laz S,Le Goaziou A,Henneke Gdoi
10.1128/JB.00268-10subject
Has Abstractpub_date
2010-07-01 00:00:00pages
3689-98issue
14eissn
0021-9193issn
1098-5530pii
JB.00268-10journal_volume
192pub_type
杂志文章abstract::FtsK is essential for Escherichia coli cell division. We report that cells lacking the C terminus of FtsK are defective in chromosome segregation as well as septation, often exhibiting asymmetrically positioned nucleoids and large anucleate regions. Combining the corresponding truncated ftsK gene with a mukB null muta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
abstract::The nifV and leuA genes, which encode homocitrate synthase and alpha-isopropylmalate synthase, respectively, were cloned from the filamentous cyanobacterium Anabaena sp. strain PCC 7120 by a PCR-based strategy. Since the N-terminal parts of NifV and LeuA from other bacteria are highly similar to each other, a single p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.9.2930-2937.1997
更新日期:1997-05-01 00:00:00
abstract::Some strains of Bradyrhizobium japonicum have the ability to catabolize indole-3-acetic acid. Indoleacetic acid (IAA), 4-chloro-IAA (4-Cl-IAA), and 5-Cl-IAA were metabolized to different extents by strains 61A24 and 110. Metabolites were isolated and analyzed by high-performance liquid chromatography and conventional ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.20.5762-5766.1995
更新日期:1995-10-01 00:00:00
abstract::The lic3 locus of Haemophilus influenzae consists of four open reading frames. The derived amino acid sequences of orf2 and orf4 exhibit homology to Escherichia coli GalE and AdK, respectively. The functions of orf1 and orf3 remain unknown. orf1 contains multiple tandem repeats of the tetrameric DNA sequence CAAT near...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.22.7245-7252.1992
更新日期:1992-11-01 00:00:00
abstract::Orthologs of RecG and RuvABC are highly conserved among prokaryotes; in Escherichia coli, they participate in independent pathways that branch migrate Holliday junctions during recombinational DNA repair. RecG also has been shown to directly convert stalled replication forks into Holliday junctions. The bacterium Heli...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01848-07
更新日期:2008-06-01 00:00:00
abstract::Site-directed mutagenesis has previously been used to construct Escherichia coli dnaK mutants encoding proteins that are altered at the site of in vitro phosphorylation (J. S. McCarty and G. C. Walker, Proc. Natl. Acad. Sci. USA 88:9513-9517, 1991). These mutants are unable to autophosphorylate and are severely defect...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.3.764-780.1994
更新日期:1994-02-01 00:00:00
abstract::Specific labeling of a single row of cellulose-synthesizing complexes (terminal complexes, TC subunits, TCs, or TC arrays) in Acetobacter xylinum by antibodies raised against a 93-kDa protein (the cyclic dignanylic acid-binding protein) has been demonstrated by using the sodium dodecyl sulfate (SDS)-freeze-fracture la...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.19.5668-5674.2001
更新日期:2001-10-01 00:00:00
abstract::The R factor NR1 consists of two components: a resistance transfer factor which harbors the tetracycline resistance genes (RTF-TC) and the r-determinants component which harbors the other drug resistance genes. Using partial denaturation mapping it is possible to distinguish the RTF-TC region from the r-determinants r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.123.3.1035-1042.1975
更新日期:1975-09-01 00:00:00
abstract::We recently demonstrated that Pseudomonas aeruginosa PAO1 undergoes a pronounced phenotypic change when introduced into the intestines of rats during surgical injury. Recovered strains displayed a specific phenotype (termed the P2 phenotype) characterized by altered pyocyanin production, high collagenase activity, hig...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01050-13
更新日期:2014-01-01 00:00:00
abstract::The major facilitator superfamily includes a large collection of evolutionarily related proteins that have been implicated in the transport of a variety of solutes and metabolites across the membranes of organisms ranging from bacteria to humans. We have recently reported the three-dimensional structure, at 6.5 A reso...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.5.1712-1718.2003
更新日期:2003-03-01 00:00:00
abstract::The Caulobacter crescentus DNA adenine methyltransferase CcrM and its homologs in the alpha-Proteobacteria are essential for viability. CcrM is 34% identical to the yhdJ gene products of Escherichia coli and Salmonella enterica. This study provides evidence that the E. coli yhdJ gene encodes a DNA adenine methyltransf...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01854-06
更新日期:2007-06-01 00:00:00
abstract::Lysozyme is an important component of the innate immune system. It functions by hydrolyzing the peptidoglycan (PG) layer of bacteria. The human pathogen Listeria monocytogenes is intrinsically lysozyme resistant. The peptidoglycan N-deacetylase PgdA and O-acetyltransferase OatA are two known factors contributing to it...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00553-20
更新日期:2021-01-25 00:00:00
abstract::The region upstream of the Escherichia coli bgl operon is an insertion hot spot for several transposons. Elements as distantly related as Tn1, Tn5, and phage Mu home in on this location. To see what characteristics result in a high-affinity site for transposition, we compared in vivo and in vitro Mu transposition patt...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.11.3328-3335.2001
更新日期:2001-06-01 00:00:00
abstract::The inner membrane TET (TetA) protein, which is involved in Tn10-mediated microbial tetracycline resistance, consists of two domains, alpha and beta, both of which are needed for tetracycline resistance and efflux (M.S. Curiale, L.M. McMurry, and S.B. Levy, J. Bacteriol. 157:211-217, 1984). Since tetracycline-sensitiv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.4.1715-1720.1988
更新日期:1988-04-01 00:00:00
abstract::The genetic organization of the foc gene cluster has been studied; six genes involved in the biogenesis of F1C fimbriae were identified. focA encodes the major fimbrial subunit, focC encodes a product that is indispensable for fimbria formation, focG and focH encode minor fimbrial subunits, and focI encodes a protein ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.2.1114-1120.1990
更新日期:1990-02-01 00:00:00
abstract::Zymomonas mobilis ZM401 is a flocculating strain which can be self-immobilized within fermentors for a high-cell-density culture to improve ethanol productivity, as well as high-gravity fermentation to increase ethanol titer, due to its improved ethanol tolerance associated with the morphological change. Here, we repo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01947-12
更新日期:2012-12-01 00:00:00
abstract::The AOX1 gene, which encodes an alternative oxidase, was isolated from the genomic DNA library of Candida albicans. The gene encodes a polypeptide consisting of 379 amino acids with a calculated molecular mass of 43,975 Da. The aox1/aox1 mutant strain did not show cyanide-resistant respiration under normal conditions ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.13.4098-4102.1999
更新日期:1999-07-01 00:00:00
abstract::A combined physical and genetic map of the Serpulina hyodysenteriae B78T genome was constructed by using pulsed-field gel electrophoresis and DNA blot hybridizations. The S. hyodysenteriae genome is a single circular chromosome about 3.2 Mb in size. The physical map of the chromosome was constructed with the restricti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.4.1087-1092.1994
更新日期:1994-02-01 00:00:00
abstract::This study shows that sequential introduction of drug resistance mutations substantially increased enzyme production in Paenibacillus agaridevorans The triple mutant YT478 (rsmG Gln225→stop codon, rpsL K56R, and rpoB R485H), generated by screening for resistance to streptomycin and rifampin, expressed a 1,100-fold-lar...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00188-18
更新日期:2018-08-10 00:00:00
abstract::Sinorhizobium meliloti strains lacking BacA function are impaired in symbiosis with alfalfa host plants and display altered sensitivities to a number of compounds relative to wild-type strains. With the goal of finding clues to the currently unknown biological function(s) of BacA, we carried out a genetic analysis to ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.21.6444-6453.2001
更新日期:2001-11-01 00:00:00
abstract::Pseudomonas aeruginosa isolates from chronic lung infections often overproduce alginate, giving rise to the mucoid phenotype. Isolation of mucoid strains from chronic lung infections correlates with a poor patient outcome. The most common mutation that causes the mucoid phenotype is called mucA22 and results in a trun...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00445-20
更新日期:2020-09-23 00:00:00
abstract::The lipoteichoic acids were isolated from phenol extracts of four Listeria strains representing serotypes 4a, 4b, 6a, and 6 to compare the differences in structure of amphiphilic polysaccharides from various serotypes of Listeria spp. The lipoteichoic acids from the four strains examined had the same structure in both...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.1.115-122.1986
更新日期:1986-10-01 00:00:00
abstract::Kinsky, Stephen C. (Washington University, St. Louis, Mo.). Induction and repression of nitrate reductase in Neurospora crassa. J. Bacteriol. 82:898-904. 1961.-Techniques are described for studying induced enzyme formation in Neurospora crassa. The effects of various parameters (time, pH, nitrate concentration, etc.) ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.6.898-904.1961
更新日期:1961-12-01 00:00:00
abstract::Ikeda, Y. (University of Tokyo, Tokyo, Japan) and T. Iijima. Heterocaryosis-like phenomenon in Bacillus subtilis. J. Bacteriol. 83:1025-1028. 1962.-When a mixture of methionine-dependent, histidine-dependent, streptomycin-resistant cells and tryptophan-dependent, streptomycin-sensitive cells of Bacillus subtilis K is ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.5.1025-1028.1962
更新日期:1962-05-01 00:00:00
abstract::Amino acid starvation allows limited synthesis of deoxyribonucleic acid (DNA) in Bacillus subtilis strain W23. DNA synthesis increased by about 30% after leucine starvation and by about 60% after histidine starvation. Genetic analysis on the DNA synthesized after amino acid starvation showed that all genetic markers e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.2.595-603.1971
更新日期:1971-02-01 00:00:00
abstract::The cold shock response of Escherichia coli is elicited by downshift of temperature from 37 degrees C to 15 degrees C and is characterized by induction of several cold shock proteins, including CsdA, during the acclimation phase. CsdA, a DEAD-box protein, has been proposed to participate in a variety of processes, suc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00655-07
更新日期:2007-08-01 00:00:00
abstract::The O antigen of the Shigella flexneri lipopolysaccharide (LPS) is an important virulence determinant and immunogen. We have isolated S. flexneri mutants which produce a semi-rough LPS by using an O-antigen-specific phage, Sf6c. Western immunoblotting was used to show that the LPS produced by the semi-rough mutants co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.3.733-747.1994
更新日期:1994-02-01 00:00:00
abstract::Grixazone (GX), which is a diffusible yellow pigment containing a phenoxazinone chromophore, is one of the secondary metabolites under the control of A-factor (2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone) in Streptomyces griseus. GX production is also induced by phosphate starvation. The whole biosynthesis gen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00055-07
更新日期:2007-05-01 00:00:00
abstract::The SOS response to DNA damage in bacteria is a well-known component of the complex transcriptional responses to genotoxic environmental stresses such as exposure to reactive oxygen species, alkylating agents, and many of the antibiotics targeting DNA replication. However, bacteria such as Bacillus subtilis also respo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00210-10
更新日期:2010-08-01 00:00:00
abstract::The growth of tubercle bacilli in serum samples of untreated animals depends upon the availability of ionic iron which serves as a growth factor in supporting bacillary multiplication. The amount of available iron in serum is determined by the ratio between iron-saturated and iron-free transferrin; a low value for the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.1.64-70.1969
更新日期:1969-10-01 00:00:00