Abstract:
:Kinsky, Stephen C. (Washington University, St. Louis, Mo.). Induction and repression of nitrate reductase in Neurospora crassa. J. Bacteriol. 82:898-904. 1961.-Techniques are described for studying induced enzyme formation in Neurospora crassa. The effects of various parameters (time, pH, nitrate concentration, etc.) on the induction of nitrate reductase were investigated. It was demonstrated that NH(4) (+), which is the end product of the metabolic sequence initiated by nitrate reductase, repressed formation of the enzyme. Parallel to the formation of nitrate reductase there was an increase in reduced triphosphopyridine nucleotide (TPNH)-cytochrome c reductase activity. The relationship of these two activities to each other and to the constitutive TPNH-cytochrome c reductase present in noninduced mycelia is discussed.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
KINSKY SCdoi
10.1128/JB.82.6.898-904.1961keywords:
subject
Has Abstractpub_date
1961-12-01 00:00:00pages
898-904eissn
0021-9193issn
1098-5530journal_volume
82pub_type
杂志文章abstract::We have utilized transposon mutagenesis to obtain insertional mutations in Providencia stuartii that activate the chromosomal aac(2')-la gene. Two closely linked mini-Tn5Cm insertions were obtained in a locus designated aarA, and a single insertion was obtained in a separate locus, aarC. Nucleotide sequence analysis, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.16.5140-5144.1994
更新日期:1994-08-01 00:00:00
abstract::The three-dimensional structure of the nickel-containing hydrogenase from Thiocapsa roseopersicina has been determined at a resolution of 2 nm in the plane and 4 nm in the vertical direction by electron microscopy and computerized image processing on microcrystals of the enzyme. The enzyme forms a large ring-shaped co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.8.2576-2580.1991
更新日期:1991-04-01 00:00:00
abstract::We have found, using a newly developed genetic method, a protein (named Cnu, for oriC-binding nucleoid-associated) that binds to a specific 26-base-pair sequence (named cnb) in the origin of replication of Escherichia coli, oriC. Cnu is composed of 71 amino acids (8.4 kDa) and shows extensive amino acid identity to a ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.20.6998-7008.2005
更新日期:2005-10-01 00:00:00
abstract::Rhodococcus equi is a facultative intracellular pathogen which proliferates rapidly in both manure-enriched soil and alveolar macrophages. Although both environments are characterized by extremely low concentrations of free iron, very little is known regarding the strategies employed by R. equi to thrive under these c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.10.3438-3444.2005
更新日期:2005-05-01 00:00:00
abstract::Conjugative transfer and replacement of hundreds of kilobases of a bacterial chromosome can occur in vitro, but replacements in nature are either an order of magnitude smaller or involve the movement of mobile genetic elements. We discovered that two lineages of Staphylococcus aureus, including a pandemic methicillin-...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.4.1060-1064.2004
更新日期:2004-02-01 00:00:00
abstract::In this study, we identified and characterized the enzymatic properties of MG_186, a calcium-dependent Mycoplasma genitalium nuclease. MG_186 displays the hallmarks of nucleases, as indicated by its amino acid sequence similarity to other nucleases. We cloned, UGA corrected, expressed, purified, and demonstrated that ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00401-10
更新日期:2010-10-01 00:00:00
abstract::The kinetics of beta-lactamase induction in Pseudomonas maltophilia IID1275/873 were investigated. Upon induction with beta-lactam antibiotics, a correlation was seen between the increase in specific beta-lactamase activity and the generation time, as well as the concentration of inducer in the medium. The specific be...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.1.483-487.1989
更新日期:1989-01-01 00:00:00
abstract::Bacillus subtilis produces a 35-kDa cell wall hydrolase, CwlF, during vegetative growth. The CwlF protein was extracted from B. subtilis cwlB sigD mutant cells and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. N-terminal amino acid sequencing revealed that its sequence is completely identical...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.9.2549-2555.1998
更新日期:1998-05-01 00:00:00
abstract::Nitroakridin 3582 (NA) formed complexes with native deoxyribonucleic acid (DNA) and with transfer ribonucleic acid (tRNA) species from Escherichia coli. Spectrophotometric titrations of NA with these nucleic acids produced numerical results from which nonlinear adsorption isotherms were derived. These curves indicated...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.3.1026-1033.1971
更新日期:1971-12-01 00:00:00
abstract:UNLABELLED:The latent period of phage T4, normally ∼25 min, can be extended indefinitely if the infected cell is superinfected after 5 min. This phenomenon, designated lysis inhibition (LIN), was first described in the 1940s and is genetically defined by mutations in diverse T4 r genes. RI, the main effector of LIN, ha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00294-16
更新日期:2016-08-25 00:00:00
abstract::Synthetic analogs of enterochelin (enterobactin) were tested for their ability to support the growth of Escherichia coli K-12 under iron-limiting conditions. The cyclic compound MECAM [1,3,5-N.N'; N"-tris-(2,3-dihydroxybenzoyl)-triamino-methylbenzene] and its N-methyl derivative Me3MECAM promoted growth, whereas the 2...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.1.109-115.1983
更新日期:1983-01-01 00:00:00
abstract::Forty-nine human and environmental isolates of Mycobacterium intracellulare and Mycobacterium scrofulaceum were tested for their ability to grow on uric acid and a number of its degradation products. Nearly all (88 to 90%) strains used uric acid or allantoin as a sole nitrogen source; fewer (47 to 69%) used allantoate...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.1.36-39.1983
更新日期:1983-07-01 00:00:00
abstract::MinD binds to phospholipid vesicles in the presence of ATP and is released by MinE, which stimulates the MinD ATPase. Membrane binding requires a short conserved C-terminal region, which has the potential to form an amphipathic helix. This finding has led to a model in which the binding of ATP regulates the formation ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.15.4326-4335.2003
更新日期:2003-08-01 00:00:00
abstract::Iron-sulfur flavoproteins (ISF) constitute a widespread family of redox-active proteins in anaerobic prokaryotes. Based on sequence homologies, their overall structure is expected to be similar to that of flavodoxins, but in addition to a flavin mononucleotide cofactor they also contain a cubane-type [4Fe:4S] cluster....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.11.3848-3854.2005
更新日期:2005-06-01 00:00:00
abstract::The Dps-like peroxide resistance protein (Dpr) is essential for H2O2 stress tolerance and aerobic growth of the oral pathogen Streptococcus mutans Dpr accumulates during oxidative stress, protecting the cell by sequestering iron ions and thereby preventing the generation of toxic hydroxyl radicals that result from the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00062-18
更新日期:2018-06-25 00:00:00
abstract::Inhibition of sterol uptake in Saccharomyces cerevisiae sterol auxotroph FY3 (alpha hem1 erg7 ura) by delta-aminolevulinic acid (ALA) is dependent on the ability of the organism to synthesize heme from ALA. Sterol-depleted cells not exposed to ALA or strain PFY3 cells, with a double heme mutation, exposed to ALA did n...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.8.3707-3711.1987
更新日期:1987-08-01 00:00:00
abstract::The bacterium Photorhabdus luminescens is a symbiont of the entomopathogenic nematode Heterorhabditis bacteriophora. The nematode requires the bacterium for infection of insect larvae and as a substrate for growth and reproduction. The nematodes do not grow and reproduce in insect hosts or on artificial media in the a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.10.3117-3126.2001
更新日期:2001-05-01 00:00:00
abstract::Lipopolysaccharide (LPS) fractions were isolated from three species of blue-green algae of the genus Phormidium, namely, P. africanum, P. laminosum, and P. uncinatum, by using a phenol-water procedure followed by exhaustive extraction with ammonium oxalate. The materials obtained were shown to be closely related bioch...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.1.1-3.1977
更新日期:1977-04-01 00:00:00
abstract::Biofilm formation is a key factor in Vibrio cholerae environmental survival and host colonization. Production of biofilm enables V. cholerae to survive and persist in aquatic environments and aids in the passage through the gastric acid barrier to allow access to the small intestine. The genes involved in biofilm form...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00014-12
更新日期:2012-06-01 00:00:00
abstract::The respiratory metabolism of Schizosaccharomyces pombe 972h(-), a fission, haplontic, "petite negative" yeast, was studied. Glucose and glycerol are good growth substrates and are oxidized under appropriate conditions. l-Lactate, ethanol, malate, and succinate are oxidized but are poor substrates for growth. d-Lactat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.1.473-481.1970
更新日期:1970-10-01 00:00:00
abstract::The thermophilic cyanobacterium Synechococcus sp. strain Y-7c-s grows at its maximum rate at a high pH (pH 8 and above) the does not show sustained growth below pH 6.5. However, rapidly growing, exponential-phase cells from high-pH cultures continued to grow rapidly for several hours after transfer to pH 6.0 or 5.0. T...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.1.237-246.1982
更新日期:1982-01-01 00:00:00
abstract::In Neurospora crassa, histidine starvation of histidine mutants resulted in derepression of histidine, tryptophan, and arginine biosynthetic enzymes. The same tripartite derepression occurred in wild-type strain 74A when it was grown in medium supplemented with 3-amino-1,2,4-triazole, an inhibitor of histidine biosynt...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.119.3.893-898.1974
更新日期:1974-09-01 00:00:00
abstract::Six mycoplasma strains, isolated under anaerobic conditions from the human oropharynx, were studied by biologic and serologic means. The strains produced nippled colonies with weak hemolytic activity for guinea pig erythrocytes on agar medium. In addition, the strains metabolized arginine with a concomitant alkaline s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.1.36-43.1969
更新日期:1969-04-01 00:00:00
abstract::Very little is known about the role of DNA repair networks in Brucella abortus and its role in pathogenesis. We investigated the roles of RecA protein, DNA repair, and SOS regulation in B. abortus. While recA mutants in most bacterial species are hypersensitive to UV damage, surprisingly a B. abortus recA null mutant ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01994-05
更新日期:2006-07-01 00:00:00
abstract::The mode of synthesis and the regulation of fructose-1,6-bisphosphatase (Fbpase), a gluconeogenic enzyme, and phosphofructokinase (PFK), a glycolytic enzyme, were investigated in Saccharomyces cerevisiae after growth in the presence of different concentrations of glucose or various gluconeogenic carbon sources. The ac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.2.647-656.1978
更新日期:1978-11-01 00:00:00
abstract::Lactobacillus pentosus MP-10 was isolated from brines of naturally fermented Aloreña green table olives. MP-10 has potential probiotic traits, including inhibition of human pathogenic bacteria, survival at low pH (1.5), and bile salt tolerance (3%). Here, we report for the first time the annotated genome sequence of L...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05171-11
更新日期:2011-09-01 00:00:00
abstract::Hydroxylamine mutagenesis was used to alter the tar gene that encodes the transmembrane Tar protein required for chemotaxis. Mutants defective in chemotaxis were selected, and the mutation was characterized by DNA sequencing. Two classes of mutations were found: nonsense and missense. The nonsense mutations were distr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.3.992-998.1986
更新日期:1986-09-01 00:00:00
abstract::Transcription of the dnaA gene of the cyanobacterium Synechocystis sp. strain PCC 6803 is light dependent and yields a monocistronic mRNA, as determined by Northern analysis. Surprisingly, mutants with inactivated dnaA were viable. In batch cultures under standard conditions, the mutants grew like the wild type and di...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.18.4946-4949.1998
更新日期:1998-09-01 00:00:00
abstract::The biosynthesis of asparaginase II in Saccharomyces cerevisiae is subject to nitrogen catabolite repression. In the present study we examined the physiological effects of glutamate auxotrophy on cellular metabolism and on the nitrogen catabolite repression of asparaginase II. Glutamate auxotrophic cells, incubated wi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.1.29-35.1982
更新日期:1982-07-01 00:00:00
abstract::We have used transcriptional and translational fusions between various vir gene promoters and the lacZ gene to study the regulation of vir genes. Like other vir promoters, the virA promoter was induced by acetosyringone in a virA virG-dependent fashion. In addition to being induced by acetosyringone, the virG promoter...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.9.4047-4054.1988
更新日期:1988-09-01 00:00:00