Structures of the iron-sulfur flavoproteins from Methanosarcina thermophila and Archaeoglobus fulgidus.

abstract：:The obligate intracellular pathogen Chlamydia trachomatis expresses a type III secretion system (T3SS) which has the potential to contribute significantly to pathogenesis. Based on a demonstrated role of type III secretion (T3S)-specific chaperones in the secretion of antihost proteins by gram-negative pathogens, we i...

journal_title：Journal of bacteriology

authors： Fields KA,Fischer ER,Mead DJ,Hackstadt T

更新日期：2005-09-01 00:00:00

abstract：:In rod-shaped bacteria, certain proteins are specifically localized to the cell poles. The nature of the positional information that leads to the proper localization of these proteins is unclear. In a screen for factors required for the localization of the Shigella sp. actin assembly protein IcsA to the bacterial pole...

journal_title：Journal of bacteriology

authors： Nilsen T,Yan AW,Gale G,Goldberg MB

更新日期：2005-09-01 00:00:00

abstract：:Twenty-four independent phosphate starvation-inducible (psi) transcriptional fusions made with Mu d1(lacZbla) were analyzed by sequencing the psi::lacZ(Mu d1) chromosomal junctions by using DNAs amplified with the polymerase chain reaction or mini-Mu cloning. Our DNA sequence analysis showed that the MuR DNA in Mu d1 ...

journal_title：Journal of bacteriology

authors： Metcalf WW,Steed PM,Wanner BL

更新日期：1990-06-01 00:00:00

abstract：:Mutants that require exogenous D-glucosamine for growth were isolated from Saccharomyces cerevisiae X2180-1A after ethyl methane sulfonate mutagenesis. Class A auxotrophs fail to grow on yeast extract-peptone-dextrose and minimal media, whereas class B auxotrophs grow on minimal medium and readily revert to grow on ye...

journal_title：Journal of bacteriology

authors： Whelan WL,Ballou CE

更新日期：1975-12-01 00:00:00

abstract：:The nucleotide sequence of the DNA mobilization region of the 5-nitroimidazole resistance plasmid pIP421, from strain BF-F239 of Bacteroides fragilis, was determined. It contains a putative origin of transfer (oriT) including three sets of inverted repeats and two sequences reminiscent of specific integration host fac...

journal_title：Journal of bacteriology

authors： Trinh S,Reysset G

更新日期：1997-06-01 00:00:00

abstract：:Rhizobium japonicum 122 DES bacteroids from soybean nodules possess an active H(2)-oxidizing system that recycles all of the H(2) lost through nitrogenase-dependent H(2) evolution. The addition of 72 muM H(2) to suspensions of bacteroids increased O(2) uptake 300% and the rate of C(2)H(2) reduction 300 to 500%. The op...

journal_title：Journal of bacteriology

authors： Emerich DW,Ruiz-Argüeso T,Ching TM,Evans HJ

更新日期：1979-01-01 00:00:00

abstract：:We have cloned the palA gene of Aspergillus nidulans, one of six genes participating in ambient pH signal transduction in a regulatory circuit mediating pH regulation of gene expression. The derived 798-residue PalA protein is 29.4% identical over its entire length to a hypothetical protein from the nematode Caenorhab...

journal_title：Journal of bacteriology

authors： Negrete-Urtasun S,Denison SH,Arst HN Jr

更新日期：1997-03-01 00:00:00

abstract：:The rcs phosphorelay pathway components were originally identified as regulators of capsule synthesis. In addition to the transmembrane sensor kinase RcsC, the RcsA coregulator, and the response regulator RcsB, two new components have been characterized, RcsD and RcsF. RcsD, the product of the yojN gene, now renamed r...

journal_title：Journal of bacteriology

authors： Majdalani N,Heck M,Stout V,Gottesman S

更新日期：2005-10-01 00:00:00

abstract：:The lethal activity of a thymineless mutant of Staphylococcus aureus Wood 46 strain has been compared with that of three naturally occurring strains: parent Wood 46, Smith, and coagulase-negative SA-13. The thymineless mutant and the parent Wood 46 strain showed a sharp decline in culturable units from the peritoneal ...

journal_title：Journal of bacteriology

authors： Mathieu LG,De Repentigny J,Turgeon S,Sonea S

更新日期：1967-07-01 00:00:00

abstract：:Enterococcus faecalis ATCC 29212, a vancomycin-sensitive strain, has been extensively used as a representative control strain for clinical and laboratory experiments. Here we report the draft genome and annotation of this strain, containing 3,027,060 bp, with a G+C content of 37.2% in 126 contigs (≥ 500 bp). ...

journal_title：Journal of bacteriology

authors： Kim EB,Kopit LM,Harris LJ,Marco ML

更新日期：2012-11-01 00:00:00

abstract：:Bacillus anthracis Sterne cured of the pXO1 plasmid had enhanced secreted protease activity during the postexponential phase but no change in hemolytic or lecithinase activities. A zymogen profile revealed at least six proteases, including serine, metal, and perhaps cysteine types. There were similar amounts of protea...

journal_title：Journal of bacteriology

authors： Aronson AI,Bell C,Fulroth B

更新日期：2005-05-01 00:00:00

abstract：:We demonstrate that expression of the UGA1, CAN1, GAP1, PUT1, PUT2, PUT4, and DAL4 genes is sensitive to nitrogen catabolite repression. The expression of all these genes, with the exception of UGA1 and PUT2, also required a functional GLN3 protein. In addition, GLN3 protein was required for expression of the DAL1, DA...

journal_title：Journal of bacteriology

authors： Daugherty JR,Rai R,el Berry HM,Cooper TG

更新日期：1993-01-01 00:00:00

abstract：:We examined the kinetics of beta-galactosidase (EC 3.2.1.23) induction in the yeast Kluyveromyces lactis. Enzyme activity began to increase 10 to 15 min, about 1/10 of a cell generation, after the addition of inducer and continued to increase linearly for from 7 to 9 cell generations before reaching a maximum, some 12...

journal_title：Journal of bacteriology

authors： Dickson RC,Markin JS

更新日期：1980-06-01 00:00:00

abstract：:Gene 1 of bacteriophage T4 has been cloned into a lambda pL expression vector, resulting in the overproduction of deoxynucleotide kinase. A procedure that includes affinity chromatography on Cibacron Blue F3GA-agarose has been used to purify milligram quantities of enzymes from a 2-liter culture. The enzyme has been p...

journal_title：Journal of bacteriology

authors： Brush GS,Bhatnagar SK,Bessman MJ

更新日期：1990-06-01 00:00:00

abstract：:Coxiella burnetii is a Gram-negative, obligate intracellular bacterial pathogen that resides within the harsh, acidic confines of a lysosome-like compartment of the host cell that is termed a parasitophorous vacuole. In this study, we characterized a thiol-specific peroxidase of C. burnetii that belongs to the atypica...

journal_title：Journal of bacteriology

authors： Hicks LD,Raghavan R,Battisti JM,Minnick MF

更新日期：2010-04-01 00:00:00

abstract：:Double-stranded, 1.9-kilobase-pair (kbp) DNA molecules were found in 18 strains representing three pathogenic races of Fusarium oxysporum f. sp. conglutinans. The DNA element (pFOXC1) from a race 1 strain and the DNA element (pFOXC2) from a race 2 strain were shown by restriction endonuclease mapping to be linear. pFO...

journal_title：Journal of bacteriology

authors： Kistler HC,Leong SA

更新日期：1986-08-01 00:00:00

abstract：:The RNA polymerase sigma subunit, sigmaH, of Bacillus subtilis is required for the transcription of genes that are induced in late-growth cultures at high cell density, including genes that function in sporulation. The expression of sigmaH-controlled genes is repressed when nutrient broth sporulation medium (Difco spo...

journal_title：Journal of bacteriology

authors： Cosby WM,Zuber P

更新日期：1997-11-01 00:00:00

abstract：:Spectinomycin dependence in Bacillus subtilis involves two mutations, one conferring drug resistance and the other producing a requirement for spectinomycin for growth. ...

journal_title：Journal of bacteriology

authors： Henkin TM,Campbell KM,Chambliss GH

更新日期：1979-03-01 00:00:00

abstract：:Pore-forming colicins are soluble bacteriocins which form voltage-gated ion channels in the inner membrane of Escherichia coli. To reach their target, these colicins first bind to a receptor located on the outer membrane and then are translocated through the envelope. Colicins are subdivided into two groups according ...

journal_title：Journal of bacteriology

authors： Duché D,Letellier L,Géli V,Bénédetti H,Baty D

更新日期：1995-09-01 00:00:00

abstract：:The inducible galactose transport system in bakers' yeast carries out the facilitated diffusion of the nonmetabolized galactose analogues d-fucose and l-arabinose. This capacity depends on the activity of the Ga 2 gene. In some strains, d-fucose and l-arabinose are also gratuitous inducers. Mutants in which the induci...

journal_title：Journal of bacteriology

authors： Cirillo VP

更新日期：1968-05-01 00:00:00

abstract：:Corynebacterium diphtheriae, a Gram-positive, aerobic bacterium, is the causative agent of diphtheria and cutaneous infections. While mechanisms required for heme iron acquisition are well known in C. diphtheriae, systems involved in the acquisition of other metals such as zinc and manganese remain poorly characterize...

journal_title：Journal of bacteriology

authors： Peng ED,Oram DM,Battistel MD,Lyman LR,Freedberg DI,Schmitt MP

更新日期：2018-04-24 00:00:00

abstract：:Cell division in Caulobacter crescentus involves constriction and fission of the inner membrane (IM) followed about 20 min later by fission of the outer membrane (OM) and daughter cell separation. In contrast to Escherichia coli, the Caulobacter Tol-Pal complex is essential. Cryo-electron microscopy images of the Caul...

journal_title：Journal of bacteriology

authors： Yeh YC,Comolli LR,Downing KH,Shapiro L,McAdams HH

更新日期：2010-10-01 00:00:00

abstract：:A class II restriction endonuclease which recognizes the same nucleotide sequence as EndoR.HaeIII has been found in four of seven isolates of Neisseria gonorrhoeae tested. ...

journal_title：Journal of bacteriology

authors： Clanton DJ,Woodward JM,Miller RV

更新日期：1978-07-01 00:00:00

abstract：:The Escherichia coli gene cluster encoding enzymatic activities responsible for the synthesis and activation of 2,3-dihydroxybenzoic acid in the formation of the catechol siderophore enterobactin was localized to a 4.2-kilobase chromosomal DNA fragment. Analysis of various subclones and transposon insertion mutations ...

journal_title：Journal of bacteriology

authors： Nahlik MS,Fleming TP,McIntosh MA

更新日期：1987-09-01 00:00:00

abstract：:A simple three-step procedure is described which yields electrophoretically homogeneous preparations of ribitol-5-phosphate dehydrogenase and xylitol-5-phosphate dehydrogenase. The former enzyme is a 115,000-molecular-weight protein composed of two subunits of identical size and is specific for its substrate, ribitol....

journal_title：Journal of bacteriology

authors： Hausman SZ,London J

更新日期：1987-04-01 00:00:00

abstract：:Growth of Streptomyces viridochromogenes on a solid glycerol-NH4NO3 salts medium was accompanied by the formation of aerial mycelia and spores. Adding 0.5% or more casein hydrolysate to the medium stimulated growth while completely repressing the formation of aerial mycelia and spores. This repression was temporary, a...

journal_title：Journal of bacteriology

authors： Coleman RH,Ensign JC

更新日期：1982-03-01 00:00:00

abstract：:Integrating conjugative elements (ICEs) of the SXT/R391 family are major contributors to the spread of antibiotic resistance genes. These elements also catalyze their own diversity by promoting inter-ICE recombination through the action of the RecA-independent homologous recombination system that they encode. Here, we...

journal_title：Journal of bacteriology

authors： Garriss G,Poulin-Laprade D,Burrus V

更新日期：2013-05-01 00:00:00

abstract：:Binding sites for the Escherichia coli protein integration host factor (IHF) include a set of conserved bases that can be summarized by the consensus sequence WATCAANNNNTTR (W is dA or dT, R is dA or dG, and N is any nucleotide). However, additional 5'-proximal bases, whose common feature is a high dA+dT content, are ...

journal_title：Journal of bacteriology

authors： Hales LM,Gumport RI,Gardner JF

更新日期：1994-05-01 00:00:00

abstract：:When Methanobacterium thermoautotrophicum cells were incubated in 50 mM potassium phosphate buffer (pH 7.0) containing 1 M sucrose and autolysate from Methanobacterium wolfei, they were transformed into protoplasts. The protoplasts, which possessed no cell wall, lysed in buffer without sucrose. Unlike whole cells, the...

journal_title：Journal of bacteriology

authors： Mountfort DO,Mörschel E,Beimborn DB,Schönheit P

更新日期：1986-11-01 00:00:00

abstract：:After heating at 65 C, ribosomes isolated from Bacillus stearothermophilus were strikingly more heat-stable than comparable preparations from Escherichia coli when tested for ability to support polyuridylic acid-directed phenylalanine incorporation at 37 C. The stability of ribosomes was also determined by measurement...

journal_title：Journal of bacteriology

authors： Friedman SM,Axel R,Weinstein IB

更新日期：1967-05-01 00:00:00