Analysis of putative Chlamydia trachomatis chaperones Scc2 and Scc3 and their use in the identification of type III secretion substrates.

abstract：:A novel tungstate and molybdate binding protein has been discovered from the hyperthermophilic archaeon Pyrococcus furiosus. This tungstate transport protein A (WtpA) is part of a new ABC transporter system selective for tungstate and molybdate. WtpA has very low sequence similarity with the earlier-characterized tran...

journal_title：Journal of bacteriology

authors： Bevers LE,Hagedoorn PL,Krijger GC,Hagen WR

更新日期：2006-09-01 00:00:00

abstract：:It previously has been shown that lkyD mutants of Salmonella typhimurium form large blebs of outer membrane over the septal and polar regions of dividing cells. To determine whether the outer membrane blebs are formed over potential sites of division even in the absence of septal ingrowth, lkyD strains were studied un...

journal_title：Journal of bacteriology

authors： Fung JC,MacAlister TJ,Weigand RA,Rothfield LI

更新日期：1980-08-01 00:00:00

abstract：:It has been clear for over sixty years that the principal method whereby cells replicate and segregate their DNA is semiconservative. It is much less clear why it should be like this rather than, say, conservative. Recently, evidence has accumulated that supports the hypothesis that one of the functions of the cell cy...

journal_title：Journal of bacteriology

authors： Norris V

更新日期：2019-05-22 00:00:00

abstract：:The complete nucleotide sequence of the hutH gene, encoding histidine ammonia-lyase (histidase), in Pseudomonas putida ATCC 12633 has been determined from the appropriate portions of the hut region that had been cloned into Escherichia coli. The resulting DNA sequence revealed an open reading frame of 1,530 base pairs...

journal_title：Journal of bacteriology

authors： Consevage MW,Phillips AT

更新日期：1990-05-01 00:00:00

abstract：:Bacteria within the Mycobacterium avium complex are prominent in the environment and are a source of serious disseminated infections in patients with AIDS. Serovars of the M. avium complex are distinguished from all other mycobacteria and from one another by the presence of highly antigenic glycolipids, the glycopepti...

journal_title：Journal of bacteriology

authors： Belisle JT,Pascopella L,Inamine JM,Brennan PJ,Jacobs WR Jr

更新日期：1991-11-01 00:00:00

abstract：:Entry into intestinal epithelial cells is an essential step in the pathogenesis of Salmonella infections. Our laboratory has previously identified a genetic locus, inv, that is necessary for efficient entry of Salmonella typhimurium into cultured epithelial cells. We have carried out a molecular and functional analysi...

journal_title：Journal of bacteriology

authors： Eichelberg K,Ginocchio CC,Galán JE

更新日期：1994-08-01 00:00:00

abstract：:Although amino acid transport has been extensively studied in bacteria during the past decade, little is known concerning the transport of those amino acids that are biosynthetic intermediates or have multiple fates within the cell. We have studied homoserine and threonine as examples of this phenomenon. Homoserine is...

journal_title：Journal of bacteriology

authors： Templeton BA,Savageau MA

更新日期：1974-03-01 00:00:00

abstract：:A conjugation system initially discovered in beta-lactamase-producing gonococci mobilized small non-selftransmissible R plasmids encoding beta-lactamase (penicillinase) production into other gonococci, Neisseria, and Escherichia coli. This conjugation system was mediated by a separate selftransmissible plasmid of 23.9...

journal_title：Journal of bacteriology

authors： Sox TE,Mohammed W,Blackman E,Biswas G,Sparling PF

更新日期：1978-04-01 00:00:00

abstract：:We have identified three transposable gene-activating elements from Pseudomonas cepacia on the basis of their abilities to increase expression of the lac genes of the broad-host-range plasmid pGC91.14 (pRP1::Tn951). When introduced into auxotrophic derivatives of P. cepacia 249 (ATCC 17616), this plasmid failed to con...

journal_title：Journal of bacteriology

authors： Wood MS,Lory C,Lessie TG

更新日期：1990-04-01 00:00:00

abstract：:Broad-host-range IncP and IncQ plasmids have been transferred to the aerobic magnetic bacterium Aquaspirillum sp. strain AMB-1. Conjugal matings with Escherichia coli S17-1 allowed high-frequency transfer of the RK2 derivative pRK415 (4.5 x 10(-3) transconjugant per recipient cell) and the RSF1010 derivative pKT230 (3...

journal_title：Journal of bacteriology

authors： Matsunaga T,Nakamura C,Burgess JG,Sode K

更新日期：1992-05-01 00:00:00

abstract：:Protein phosphorylation was demonstrated in Bradyrhizobium japonicum bacteroids in vivo and in cultures in vivo and in vitro. Comparison of in vivo-labeled phosphoproteins of bacteroids and of cultured cells showed differences in both the pattern and intensity of labeling. In cultured cells, comparison of the labeling...

journal_title：Journal of bacteriology

authors： Karr DB,Emerich DW

更新日期：1989-06-01 00:00:00

abstract：:Bacteriophage GA-1 infects Bacillus sp. strain G1R and has a linear double-stranded DNA genome with a terminal protein covalently linked to its 5' ends. GA-1 protein p6 is very abundant in infected cells and binds DNA with no sequence specificity. We show here that it binds in vivo to the whole viral genome, as detect...

journal_title：Journal of bacteriology

authors： Alcorlo M,Salas M,Hermoso JM

更新日期：2007-11-01 00:00:00

abstract：:Two deoxyribonucleic acid (DNA)-dependent ribonucleic acid (RNA) polymerases (I, II) have been solubilized from isolated Saccharomyces cerevisiae nuclei. The enzymes can be separated by chromatography on O-diethylaminoethyl Sephadex. Both enzymes are active with high-molecular-weight nuclear yeast DNA, although RNA po...

journal_title：Journal of bacteriology

authors： Sebastian J,Bhargava MM,Halvorson HO

更新日期：1973-04-01 00:00:00

abstract：:The Rhizobium meliloti bacA gene encodes a function that is essential for bacterial differentiation into bacteroids within plant cells in the symbiosis between R. meliloti and alfalfa. An Escherichia coli homolog of BacA, SbmA, is implicated in the uptake of microcin B17, microcin J25 (formerly microcin 25), and bleom...

journal_title：Journal of bacteriology

authors： Ichige A,Walker GC

更新日期：1997-01-01 00:00:00

abstract：:Transfer of conjugative plasmids requires relaxases, proteins that cleave one plasmid strand sequence specifically. The F plasmid relaxase TraI (1,756 amino acids) is also a highly processive DNA helicase. The TraI relaxase activity is located within the N-terminal approximately 300 amino acids, while helicase motifs ...

journal_title：Journal of bacteriology

authors： Dostál L,Schildbach JF

更新日期：2010-07-01 00:00:00

abstract：:A mutant of Pseudomonas aeruginosa severely deficient in outer membrane protein F levels was isolated by screening heavily mutagenized strains for membrane protein alterations on sodium dodecyl sulphate-polyacrylamide gel electrophoresis. To provide a basis for phenotypic comparison, three independent spontaneous reve...

journal_title：Journal of bacteriology

authors： Nicas TI,Hancock RE

更新日期：1983-01-01 00:00:00

abstract：:Unlike mutations to streptomycin resistance, which restrict the function of an SU II amber suppressor, mutations to spectinomycin resistance have no restrictive effect. Furthermore, mutations to spectinomycin resistance counteract the restrictive action of streptomycin-resistant mutations when combined in a single str...

journal_title：Journal of bacteriology

authors： Kuwano M,Endo H,Ohnishi Y

更新日期：1969-02-01 00:00:00

abstract：:Integration host factor (IHF) is a small, basic protein that is needed for efficient recombination of bacteriophage lambda, as well as for other host and viral functions. We have constructed strains in which the two subunits of IHF, encoded by the himA and hip genes of Escherichia coli, are expressed under the control...

journal_title：Journal of bacteriology

authors： Nash HA,Robertson CA,Flamm E,Weisberg RA,Miller HI

更新日期：1987-09-01 00:00:00

abstract：:Bacteroides fragilis synthesizes eight distinct capsular polysaccharides, more than any described bacterium outside the order Bacteroidales. Here, we show that this organism also produces a high-molecular-weight extracellular polysaccharide (EPS). Expression of the EPS results in the formation of a large polysaccharid...

journal_title：Journal of bacteriology

authors： Chatzidaki-Livanis M,Coyne MJ,Roche-Hakansson H,Comstock LE

更新日期：2008-02-01 00:00:00

abstract：:The Methanococcus maripaludis energy-conserving hydrogenase B (Ehb) generates low potential electrons required for autotrophic CO(2) assimilation. To analyze the importance of individual subunits in Ehb structure and function, markerless in-frame deletions were constructed in a number of M. maripaludis ehb genes. Thes...

journal_title：Journal of bacteriology

authors： Major TA,Liu Y,Whitman WB

更新日期：2010-08-01 00:00:00

abstract：:Pseudomonas sp. strain PCL1171 displays colony phase variation between opaque phase I and translucent phase II colonies, thereby regulating the production of secondary metabolites and exoenzymes. Complementation and sequence analysis of 26 phase II mutants and of 13 wild-type phase II sectors growing out of phase I co...

journal_title：Journal of bacteriology

authors： van den Broek D,Chin-A-Woeng TF,Bloemberg GV,Lugtenberg BJ

更新日期：2005-01-01 00:00:00

abstract：:The lipopolysaccharide (LPS) from a Rhizobium phaseoli mutant, CE109, was isolated and compared with that of its wild-type parent, CE3. A previous report has shown that the mutant is defective in infection thread development, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis shows that it has an altered LP...

journal_title：Journal of bacteriology

authors： Carlson RW,Kalembasa S,Turowski D,Pachori P,Noel KD

更新日期：1987-11-01 00:00:00

abstract：:We report a draft sequence of the genome of Gordonia neofelifaecis NRRL B-59395, a cholesterol-degrading actinomycete isolated from fresh feces of a clouded leopard (Neofelis nebulosa). As predicted, the reported genome contains several gene clusters for cholesterol degradation. This is the second available genome seq...

journal_title：Journal of bacteriology

authors： Ge F,Li W,Chen G,Liu Y,Zhang G,Yong B,Wang Q,Wang N,Huang Z,Li W,Wang J,Wu C,Xie Q,Liu G

更新日期：2011-09-01 00:00:00

abstract：:The genomic organization of the acidophilic chemolithoautotrophic bacterium Thiobacillus ferrooxidans ATCC 21834 has been studied by pulsed-field gel electrophoresis (PFGE). Analysis of its intact DNA, as well as the restriction patterns obtained with several endonucleases, allowed the characterization of one circular...

journal_title：Journal of bacteriology

authors： Irazabal N,Marín I,Amils R

更新日期：1997-03-01 00:00:00

abstract：UNLABELLED:During biosynthesis of [NiFe]-hydrogenase 2 (Hyd-2) of Escherichia coli, a 15-amino-acid C-terminal peptide is cleaved from the catalytic large subunit precursor, pro-HybC. This peptide is removed only after NiFe(CN)2CO cofactor insertion by the Hyp accessory protein machinery has been completed, suggesting ...

journal_title：Journal of bacteriology

authors： Thomas C,Muhr E,Sawers RG

更新日期：2015-09-01 00:00:00

abstract：:The effects of the free bile acids (FBAs) cholic acid (CA), deoxycholic acid (DCA), and chenodeoxycholic acid on the bioenergetics and growth of lactobacilli and bifidobacteria were investigated. It was found that these FBAs reduced the internal pH levels of these bacteria with rapid and stepwise kinetics and, at cert...

journal_title：Journal of bacteriology

authors： Kurdi P,Kawanishi K,Mizutani K,Yokota A

更新日期：2006-03-01 00:00:00

abstract：:MobB is a small protein encoded by the broad-host-range plasmid R1162 and required for efficient mobilization of its DNA during conjugation. The protein was shown previously to stabilize the relaxosome, the complex of plasmid DNA and mobilization proteins at the origin of transfer (oriT). We have generated in-frame mo...

journal_title：Journal of bacteriology

authors： Perwez T,Meyer RJ

更新日期：1999-04-01 00:00:00

abstract：:Bacteriocin JF246 has been purified from mitomycin C-induced Serratia marcescens cells by salt extraction, ammonium sulfate fractionation, and chromatography on QAE-Sephadex and SP-Sephadex. The purified material is homogeneous on polyacrylamide gel electrophoresis in the presence of 2% sodium dodecyl sulfate or 6 m u...

journal_title：Journal of bacteriology

authors： Foulds J

更新日期：1972-06-01 00:00:00

abstract：:The Escherichia coli beta-galactosidase enzyme was used as a reporter molecule for genetic fusions in Rhodobacter capsulatus. DNA fragments that were from the upstream region of the hydrogenase structural operon hupSLM and contained 5' hupS sequences were fused in frame to a promoterless lacZ gene, yielding fusion pro...

journal_title：Journal of bacteriology

authors： Colbeau A,Vignais PM

更新日期：1992-07-01 00:00:00

abstract：:Streptomyces coelicolor Müller contains two superoxide dismutases (SODs), nickel-containing (NiSOD) and iron- and zinc-containing SOD (FeZnSOD). The sodF gene encoding FeZnSOD was isolated by using PCR primers corresponding to the N-terminal peptide sequence of the purified FeZnSOD and a C-terminal region conserved am...

journal_title：Journal of bacteriology

authors： Kim EJ,Chung HJ,Suh B,Hah YC,Roe JH

更新日期：1998-04-01 00:00:00