Bacteriophage T4 deoxynucleotide kinase: gene cloning and enzyme purification.

abstract：:NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) of Paracoccus denitrificans has been purified as a tetramer with a relative molecular mass of 150 kDa. The gene encoding GD-FALDH (flhA) has been isolated, sequenced, and mutated by insertion of a kanamycin resistance gene. The mutant strain is not a...

journal_title：Journal of bacteriology

authors： Ras J,Van Ophem PW,Reijnders WN,Van Spanning RJ,Duine JA,Stouthamer AH,Harms N

更新日期：1995-01-01 00:00:00

abstract：:Gene sequences encoding the enzymes UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) from many bacterial sources were analyzed. It was shown that whereas gram-negative bacteria have only one murA gene, gram-positive bacteria have two distinct genes encoding these enzymes which have possibly arisen from gene dupl...

journal_title：Journal of bacteriology

authors： Du W,Brown JR,Sylvester DR,Huang J,Chalker AF,So CY,Holmes DJ,Payne DJ,Wallis NG

更新日期：2000-08-01 00:00:00

abstract：:The group A streptococcus (GAS) Streptococcus pyogenes is known to cause self-limiting purulent infections in humans. The role of GAS pili in host cell adhesion and biofilm formation is likely fundamental in early colonization. Pilus genes are found in the FCT (fibronectin-binding protein, collagen-binding protein, an...

journal_title：Journal of bacteriology

authors： Kimura KR,Nakata M,Sumitomo T,Kreikemeyer B,Podbielski A,Terao Y,Kawabata S

更新日期：2012-02-01 00:00:00

abstract：:Transcription of the virG gene of Agrobacterium tumefaciens was previously shown to be expressed from two tandem promoters and to be responsive to three stimuli: plant-released phenolic compounds, phosphate starvation, and acidic media. In this report, I describe a set of deletions and other alterations of the 5' end ...

journal_title：Journal of bacteriology

authors： Winans SC

更新日期：1990-05-01 00:00:00

abstract：:We describe the identification and characterization of two genes and their gene products responsible for determining receptor binding and pilus length in type 1-piliated Escherichia coli. One gene, pilE, conferred the ability of piliated cells to agglutinate guinea pig erythrocytes. The other gene, pilF, determined pi...

journal_title：Journal of bacteriology

authors： Maurer L,Orndorff PE

更新日期：1987-02-01 00:00:00

abstract：:Aeromonas hydrophila secretes a number of degradative enzymes and toxins into the external milieu via the type II secretory pathway or secreton. ExeA is an essential component of this system and is necessary for the localization and/or multimerization of the secretin ExeD. ExeA contains two sequence motifs characteris...

journal_title：Journal of bacteriology

authors： Schoenhofen IC,Li G,Strozen TG,Howard SP

更新日期：2005-09-01 00:00:00

abstract：:In earlier studies from this laboratory, Xanthomonas campestris pv. glycines was found to exhibit a nutrition stress-related postexponential rapid cell death (RCD). The RCD was exhibited in protein-rich media but not in starch or other minimal media. This RCD in X. campestris pv. glycines was found to display features...

journal_title：Journal of bacteriology

authors： Raju KK,Gautam S,Sharma A

更新日期：2006-08-01 00:00:00

abstract：:Streptococcus suis serotype 2 is a highly invasive, extracellular pathogen in pigs with the capacity to cause severe infections in humans. This study was initiated by the finding that IgM degradation products are released after opsonization of S. suis. The objective of this work was to identify the bacterial factor re...

journal_title：Journal of bacteriology

authors： Seele J,Singpiel A,Spoerry C,von Pawel-Rammingen U,Valentin-Weigand P,Baums CG

更新日期：2013-03-01 00:00:00

abstract：:A gene required for growth and viability in recA mutants of Escherichia coli K-12 was identified. This gene, rdgB (for Rec-dependent growth), mapped near 64 min on the E. coli genetic map. In a strain carrying a temperature-sensitive recA allele, recA200, and an rdgB mutation, DNA synthesis but not protein synthesis c...

journal_title：Journal of bacteriology

authors： Clyman J,Cunningham RP

更新日期：1987-09-01 00:00:00

abstract：:Accumulation of certain nonmetabolizable sugar-phosphates (including α-methyl glucoside-6-phosphate) in Escherichia coli is growth inhibitory and elicits the glucose-phosphate stress response. The transcription factor SgrR activates transcription of the small RNA SgrS under stress conditions. SgrS represses translatio...

journal_title：Journal of bacteriology

authors： Sun Y,Vanderpool CK

更新日期：2011-01-01 00:00:00

abstract：:It has been shown previously that Bacteroides thetaiotaomicron, a human colonic anaerobe, can utilize the tissue mucopolysaccharide chondroitin sulfate as a source of carbon and energy and that the enzymes involved in this utilization are all cell associated (A. A. Salyers and M. B. O'Brien, J. Bacteriol. 143:772-780,...

journal_title：Journal of bacteriology

authors： Kuritza AP,Salyers AA

更新日期：1983-03-01 00:00:00

abstract：:Analysis of the genome sequence of Enterococcus faecalis allowed the identification of two genes whose protein products showed 33 and 34% identity with those of sigV and yrhM of Bacillus subtilis, respectively. These genes, named sigV and rsiV, are predicted to encode members of the extracytoplasmic function subfamily...

journal_title：Journal of bacteriology

authors： Benachour A,Muller C,Dabrowski-Coton M,Le Breton Y,Giard JC,Rincé A,Auffray Y,Hartke A

更新日期：2005-02-01 00:00:00

abstract：:We describe here the cloning of single EcoRI fragments from the tra region of F DNA using ColE1::Tn3 as vector. These plasmids, as well as the series of Skurray et al. (Proc. Natl. Acad. Sci. U.S.A. 73:64-68, 1976), have been used to refine the map positions of tra cistrons on the F factor as well as to define a new D...

journal_title：Journal of bacteriology

authors： Achtman M,Skurray RA,Thompson R,Helmuth R,Hall S,Beutin L,Clark AJ

更新日期：1978-03-01 00:00:00

abstract：:Haight, Rodger D. (Oregon State University, Corvallis), and Richard Y. Morita. Thermally induced leakage from Vibrio marinus, an obligately psychrophilic bacterium. J. Bacteriol. 92:1388-1393. 1966.-Leakage of various cellular components into the surrounding menstruum occurred when Vibrio marinus was subjected to temp...

journal_title：Journal of bacteriology

authors： Haight RD,Morita RY

更新日期：1966-11-01 00:00:00

abstract：:The genes for protein synthesis elongation factors Tu and G were cloned from the cyanobacterium Anacystis nidulans. The locations of these genes were mapped within the cloned DNA fragment by hybridization with Escherichia coli probes. The organization of the cloned fragment and the DNA flanking it in the A. nidulans c...

journal_title：Journal of bacteriology

authors： Mickel FS,Spremulli LL

更新日期：1986-04-01 00:00:00

abstract：:Cellulolytic strains of Bacillus stearothermophilus were isolated from nature and screened for the presence of activities associated with the degradation of plant cell walls. One isolate (strain XL-65-6) which exhibited strong activities with 4-methylumbelliferyl-beta-D-glucopyranoside (MUG) and 4-methylumbelliferyl-b...

journal_title：Journal of bacteriology

authors： Lai X,Ingram LO

更新日期：1993-10-01 00:00:00

abstract：:Granular structures containing inorganic polyphosphate were found in Micrococcus lysodeikticus. These structures were isolated by fractionation of the bacterial extract obtained by lysing the organisms with lysozyme. The composition of the fraction which was enriched with these structures was found to be: protein, 24%...

journal_title：Journal of bacteriology

authors： Friedberg I,Avigad G

更新日期：1968-08-01 00:00:00

abstract：:Staphylococcus aureus was shown to transport iron complexed to a variety of hydroxamate type siderophores, including ferrichrome, aerobactin, and desferrioxamine. An S. aureus mutant defective in the ability to transport ferric hydroxamate complexes was isolated from a Tn917-LTV1 transposon insertion library after sel...

journal_title：Journal of bacteriology

authors： Sebulsky MT,Hohnstein D,Hunter MD,Heinrichs DE

更新日期：2000-08-01 00:00:00

abstract：:A Synechocystis sp. strain PCC 6803 mutant lacking CtaI, a main subunit of cytochrome c oxidase, is not capable of growing at light intensities below 5 micromol photons m(-2) s(-1), presumably due to an overreduced plastoquinone pool in the thylakoid membrane. Upon selection for growth at light intensities below 5 mic...

journal_title：Journal of bacteriology

authors： Kufryk GI,Vermaas WF

更新日期：2006-02-01 00:00:00

abstract：:Serratia plymuthica strain PRI-2C is a rhizosphere bacterial strain with antagonistic activity against different plant pathogens. Here we present the 5.39-Mb (G+C content, 55.67%) draft genome sequence of S. plymuthica strain PRI-2C with the aim of providing insight into the genomic basis of its antagonistic activity....

journal_title：Journal of bacteriology

authors： Garbeva P,van Elsas JD,de Boer W

更新日期：2012-08-01 00:00:00

abstract：:The mobile element IS256 causes phase variation of biofilm formation in Staphylococcus epidermidis by insertion and precise excision from the icaADBC operon. Precise excision, i.e., removal of the target site duplications (TSDs) and restoration of the original DNA sequence, occurs rarely but independently of functiona...

journal_title：Journal of bacteriology

authors： Hennig S,Ziebuhr W

更新日期：2008-02-01 00:00:00

abstract：:Nucleoside diphosphate kinase (Ndk) is a ubiquitous enzyme which functions in balancing the nucleotide pool of the cell. We have recently reported that in addition to being intracellular in both mucoid and nonmucoid Pseudomonas aeruginosa, Ndk is also secreted into the extracellular environment by mucoid P. aeruginosa...

journal_title：Journal of bacteriology

authors： Kamath S,Chen ML,Chakrabarty AM

更新日期：2000-07-01 00:00:00

abstract：:Methods were devised to purify the cell-associated, iron-binding compounds known as mycobactins from the closely related species Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum (i.e., the MAIS complex of organisms). The mycobactins from these three species showed a structure that is c...

journal_title：Journal of bacteriology

authors： Barclay R,Ewing DF,Ratledge C

更新日期：1985-11-01 00:00:00

abstract：:Catheter-related bloodstream infections due to Staphylococcus aureus are of increasing clinical importance. The pathophysiological steps leading to colonization and infection, however, are still incompletely defined. We observed growth and detachment of S. aureus biofilms in an in vitro catheter-infection model by usi...

journal_title：Journal of bacteriology

authors： Fux CA,Wilson S,Stoodley P

更新日期：2004-07-01 00:00:00

abstract：:Addition of purines to the growth medium of Escherichia coli represses synthesis of cytosine deaminase (codA) and enzymes of purine de novo synthesis. After Tn10 mutagenesis, mutants displaying derepressed levels of cytosine deaminase in the presence of hypoxanthine were isolated. One of these had simultaneously acqui...

journal_title：Journal of bacteriology

authors： Kilstrup M,Meng LM,Neuhard J,Nygaard P

更新日期：1989-04-01 00:00:00

abstract：:In Bacillus subtilis the deoxyribonucleic acid content and the extent of cell division during inhibition of chromosome replication increased as a function of the average cell mass, independent of the growth rate. At each growth rate, mass, deoxyribonucleic acid, and residual division varied in different cultures. The ...

journal_title：Journal of bacteriology

authors： Holmes M,Rickert M,Pierucci O

更新日期：1980-04-01 00:00:00

abstract：:Several biochemical parameters, including that of polyamine content, accompanying the growth of the cyanobacterium Anacystis nidulans were studied. At all stages of growth under autotrophic conditions, the organisms were found to be rich in spermidine and lacking in spermine, as is typical of procaryotic organisms. Th...

journal_title：Journal of bacteriology

authors： Ramakrishna S,Guarino L,Cohen SS

更新日期：1978-06-01 00:00:00

abstract：:Agrobacterium tumefaciens and Agrobacterium rhizogenes are related pathogens that cause crown gall and hairy root diseases, which result from integration and expression of bacterial genes in the plant genome. Single-stranded DNA (T strands) and virulence proteins are translocated into plant cells by a type IV secretio...

journal_title：Journal of bacteriology

authors： Hodges LD,Lee LY,McNett H,Gelvin SB,Ream W

更新日期：2009-01-01 00:00:00

abstract：:Glyoxal is toxic and mutagenic α-oxoaldehyde generated in vivo as an oxidation by-product of sugar metabolism. We selected glyoxal-resistant mutants from an Escherichia coli strain lacking major glyoxal-detoxifying genes, gloA and yqhD, by growing cells in medium containing a lethal concentration of glyoxal. The mutan...

journal_title：Journal of bacteriology

authors： Kwon M,Lee J,Lee C,Park C

更新日期：2012-04-01 00:00:00

abstract：:FtsZ, the bacterial tubulin homologue, is the main player in at least two distinct processes of cell division during the development of Streptomyces coelicolor A3(2). It forms cytokinetic rings and is required for the formation of both the widely spaced hyphal cross walls in the substrate mycelium and the specialized ...

journal_title：Journal of bacteriology

authors： Grantcharova N,Lustig U,Flärdh K

更新日期：2005-05-01 00:00:00