Regulation and function of Escherichia coli sugar efflux transporter A (SetA) during glucose-phosphate stress.

Abstract:

:Accumulation of certain nonmetabolizable sugar-phosphates (including α-methyl glucoside-6-phosphate) in Escherichia coli is growth inhibitory and elicits the glucose-phosphate stress response. The transcription factor SgrR activates transcription of the small RNA SgrS under stress conditions. SgrS represses translation of mRNAs encoding sugar transporters. The sgrR and sgrS genes are located directly upstream of setA, and this gene organization is conserved in numerous enteric species, prompting the hypothesis that SetA contributes to the glucose-phosphate stress response. SetA is a proton motive force-driven efflux pump capable of transporting various sugars and sugar analogs in vitro. This study demonstrates that setA expression is induced in response to glucose-phosphate stress, and this requires SgrR. Under stress conditions, setA is cotranscribed with sgrS from the sgrS promoter. A setA mutant exhibits a growth defect under stress conditions that can be complemented by setA in trans, suggesting that SetA contributes to the optimal cellular recovery from stress. Despite previous in vitro evidence that SetA can promote efflux of the stress-causing glucose analog α-methyl glucoside, in vivo data in this study indicate that SetA is not the major efflux pump responsible for removal of α-methyl glucoside under stress conditions.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Sun Y,Vanderpool CK

doi

10.1128/JB.01008-10

subject

Has Abstract

pub_date

2011-01-01 00:00:00

pages

143-53

issue

1

eissn

0021-9193

issn

1098-5530

pii

JB.01008-10

journal_volume

193

pub_type

杂志文章
  • Mutations in ampG or ampD affect peptidoglycan fragment release from Neisseria gonorrhoeae.

    abstract::Neisseria gonorrhoeae releases peptidoglycan fragments during growth. The majority of the fragments released are peptidoglycan monomers, molecules known to increase pathogenesis through the induction of proinflammatory cytokines and responsible for the killing of ciliated epithelial cells. In other gram-negative bacte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01194-07

    authors: Garcia DL,Dillard JP

    更新日期:2008-06-01 00:00:00

  • Evidence for the existence of two arginyl-transfer ribonucleic acid synthetase activities in Escherichia coli.

    abstract::Two arginyl-transfer ribonucleic acid (tRNA) synthetase (EC 6.1.1.13, arginine: ribonucleic acid ligase adenosine monophosphate) activities were found in extracts of Escherichia coli strains AB1132 and NP2. The two arginyl-tRNA synthetase activities in extracts of strain AB1132 were found to be separable by diethylami...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.2.891-894.1973

    authors: Yem DW,Williams LS

    更新日期:1973-02-01 00:00:00

  • Patterns of sequence conservation in the S-Layer proteins and related sequences in Clostridium difficile.

    abstract::Clostridium difficile is the etiological agent of antibiotic-associated diarrhea. Among the factors that may play a role in infection are S-layer proteins (SLPs). Previous work has shown these to consist mainly of two components, resulting from the cleavage of a precursor encoded by the slpA gene. The high-molecular-w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.14.3886-3897.2002

    authors: Calabi E,Fairweather N

    更新日期:2002-07-01 00:00:00

  • Purification and characterization of the N-terminal domain of ExeA: a novel ATPase involved in the type II secretion pathway of Aeromonas hydrophila.

    abstract::Aeromonas hydrophila secretes a number of degradative enzymes and toxins into the external milieu via the type II secretory pathway or secreton. ExeA is an essential component of this system and is necessary for the localization and/or multimerization of the secretin ExeD. ExeA contains two sequence motifs characteris...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.18.6370-6378.2005

    authors: Schoenhofen IC,Li G,Strozen TG,Howard SP

    更新日期:2005-09-01 00:00:00

  • Factors affecting transfection in Bacillus stearothermophilus.

    abstract::The conditions for the infection of Bacillus stearothermophilus 4S with TP-1C phage deoxyribonucleic acid (DNA) are described. Cells from log-phase cultures are the most susceptible to phage DNA infection (transfection). A cellular component (competence factor) which enhances transfection is released into the culture ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.106.3.960-965.1971

    authors: Streips UN,Welker NE

    更新日期:1971-06-01 00:00:00

  • Localization of cytochromes to the outer membrane of anaerobically grown Shewanella putrefaciens MR-1.

    abstract::In gram-negative bacteria, numerous cell functions, including respiration-linked electron transport, have been ascribed to the cytoplasmic membrane. Gram-negative bacteria which use solid substrates (e.g., oxidized manganese or iron) as terminal electron acceptors for anaerobic respiration are presented with a unique ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.11.3429-3438.1992

    authors: Myers CR,Myers JM

    更新日期:1992-06-01 00:00:00

  • Purification and chemical composition of reticulate bodies of the meningopneumonitis organisms.

    abstract::Reticulate bodies of the meningopneumonitis (MP) microorganism were purified from L cells 18 hr after infection by the combination of differential centrifugation in 30% sucrose solution and potassium tartrate density gradient centrifugation. It was ascertained by electron microscopy that purified preparations of retic...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.6.2003-2008.1967

    authors: Tamura A,Matsumoto A,Higashi N

    更新日期:1967-06-01 00:00:00

  • Conjugative plasmids of enteric bacteria from many different incompatibility groups have similar genes for single-stranded DNA-binding proteins.

    abstract::Among 30 conjugative plasmids of enteric bacteria from 23 incompatibility (Inc) groups, we found 19 (from 12 Inc groups) which can complement defects caused by a defective single-stranded DNA-binding protein of Escherichia coli K-12. The genes which are responsible for the complementation from three of these plasmids ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.1.235-241.1985

    authors: Golub EI,Low KB

    更新日期:1985-04-01 00:00:00

  • Biochemical and phylogenetic characterization of a novel diaminopimelate biosynthesis pathway in prokaryotes identifies a diverged form of LL-diaminopimelate aminotransferase.

    abstract::A variant of the diaminopimelate (DAP)-lysine biosynthesis pathway uses an LL-DAP aminotransferase (DapL, EC 2.6.1.83) to catalyze the direct conversion of L-2,3,4,5-tetrahydrodipicolinate to LL-DAP. Comparative genomic analysis and experimental verification of DapL candidates revealed the existence of two diverged fo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01381-07

    authors: Hudson AO,Gilvarg C,Leustek T

    更新日期:2008-05-01 00:00:00

  • ColE1 plasmid incompatibility: localization and analysis of mutations affecting incompatibility.

    abstract::Deletion mutants of plasmid ColE1 that involve the replication origin and adjacent regions of the plasmid have been studied to determine the mechanism by which those mutations affect the expression of plasmid incompatibility. It was observed that (i) a region of ColE1 that is involved in the expression of plasmid inco...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.139.2.608-619.1979

    authors: Hashimoto-Gotoh T,Inselburg J

    更新日期:1979-08-01 00:00:00

  • BIOLOGY OF BUDDING BACTERIA. 3. FINE STRUCTURE OF RHODOMICROBIUM AND HYPHOMICROBIUM SPP.

    abstract::Conti, S. F. (Dartmouth Medical School, Hanover, N.H.), and Peter Hirsch. Biology of budding bacteria. III. Fine structure of Rhodomicrobium and Hyphomicrobium spp. J. Bacteriol. 89:503-512. 1965.-The ultrastructure of 14 strains of hyphomicrobia, and of Rhodomicrobium vannielii, was investigated by means of electron ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.2.503-512.1965

    authors: CONTI SF,HIRSCH P

    更新日期:1965-02-01 00:00:00

  • Point mutations in a peptidoglycan biosynthesis gene cause competence induction in Haemophilus influenzae.

    abstract::We have identified three new Haemophilus influenzae mutations causing cells to exhibit extreme hypercompetence at all stages of growth. The mutations are in murE, which encodes the meso-diaminopimelate-adding enzyme of peptidoglycan synthesis. All are point mutations causing nonconservative amino acid substitutions, t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.12.3323-3330.2000

    authors: Ma C,Redfield RJ

    更新日期:2000-06-01 00:00:00

  • Klebsiella serotype 25 capsular polysaccharide: primary structure and depolymerization by a bacteriophage-borne glycanase.

    abstract::By partial acid hydrolysis, methylation and gas-liquid chromatography-mass spectrometry of the methylated monomers (as the alditol acetates), mass spectrometry of trimethylsilylated disaccharide alditols, as well as proton magnetic resonance, the primary structure of the Klebsiella serotype 25 capsular polysaccharide ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.1.366-374.1977

    authors: Niemann H,Kwiatkowski B,Westphal U,Stirm S

    更新日期:1977-04-01 00:00:00

  • Role of the Nfo (YqfS) and ExoA apurinic/apyrimidinic endonucleases in protecting Bacillus subtilis spores from DNA damage.

    abstract::The Bacillus subtilis enzymes ExoA and Nfo (originally termed YqfS) are endonucleases that can repair apurinic/apyrimidinic (AP) sites and strand breaks in DNA. We have analyzed how the lack of ExoA and Nfo affects the resistance of growing cells and dormant spores of B. subtilis to a variety of treatments, some of wh...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.21.7374-7381.2005

    authors: Salas-Pacheco JM,Setlow B,Setlow P,Pedraza-Reyes M

    更新日期:2005-11-01 00:00:00

  • Disruption of VirB6 Paralogs in Anaplasma phagocytophilum Attenuates Its Growth.

    abstract::Many pathogenic bacteria translocate virulence factors into their eukaryotic hosts by means of type IV secretion systems (T4SS) spanning the inner and outer membranes. Genes encoding components of these systems have been identified within the order Rickettsiales based upon their sequence similarities to other prototyp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00301-20

    authors: Crosby FL,Munderloh UG,Nelson CM,Herron MJ,Lundgren AM,Xiao YP,Allred DR,Barbet AF

    更新日期:2020-11-04 00:00:00

  • Second system for potassium transport in Streptococcus faecalis.

    abstract::It has been reported that the accumulation of K+ by Streptococcus faecalis is mediated by a transport system which required both ATP and the proton motive force (Bakker and Harold, J. Biol. Chem. 255:433-440, 1980). My results indicate that S. faecalis has a second transport system for K+. The features of this system ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.150.2.506-511.1982

    authors: Kobayashi H

    更新日期:1982-05-01 00:00:00

  • Contributions of individual σB-dependent general stress genes to oxidative stress resistance of Bacillus subtilis.

    abstract::The general stress regulon of Bacillus subtilis comprises approximately 200 genes and is under the control of the alternative sigma factor σ(B). The activation of σ(B) occurs in response to multiple physical stress stimuli as well as energy starvation conditions. The expression of the general stress proteins provides ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00528-12

    authors: Reder A,Höper D,Gerth U,Hecker M

    更新日期:2012-07-01 00:00:00

  • Heterologous expression of Bartonella adhesin A in Escherichia coli by exchange of trimeric autotransporter adhesin domains results in enhanced adhesion properties and a pathogenic phenotype.

    abstract::Human-pathogenic Bartonella henselae causes cat scratch disease and vasculoproliferative disorders. An important pathogenicity factor of B. henselae is the trimeric autotransporter adhesin (TAA) Bartonella adhesin A (BadA), which is modularly constructed, consisting of a head, a long and repetitive neck-stalk module, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01461-13

    authors: Schmidgen T,Kaiser PO,Ballhorn W,Franz B,Göttig S,Linke D,Kempf VA

    更新日期:2014-06-01 00:00:00

  • RESISTANCE AND CROSS RESISTANCE OF ESCHERICHIA COLI S MUTANTS TO THE RADIOMIMETIC AGENT NITROFURAZONE.

    abstract::Woody-Karrer, Pearl (Palo Alto Medical Research Foundation, Palo Alto, Calif.) and Joseph Greenberg. Resistance and cross resistance of Escherichia coli S mutants to the radiomimetic agent nitrofurazone. J. Bacteriol. 85:1208-1216. 1963.-Cross-resistance relationships are described for 73 mutants of Escherichia coli s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.85.6.1208-1216.1963

    authors: WOODY-KARRER P,GREENBERG J

    更新日期:1963-06-01 00:00:00

  • Factors Affecting the Survival of Auxotrophs and Prototrophs of Saccharomyces cerevisiae in Mixed Populations.

    abstract::Moat, Albert G. (Hahnemann Medical College, Philadelphia, Pa.), Isabel J. Barnes, and Eleanor H. McCurley. Factors affecting the survival of auxotrophs and prototrophs of Saccharomyces cerevisiae in mixed populations. J. Bacteriol. 92:297-301. 1966.-The conditions under which the number of yeast prototrophs, as well a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.92.2.297-301.1966

    authors: Moat AG,Barnes IJ,McCurley EH

    更新日期:1966-08-01 00:00:00

  • Comparison of the membrane composition of Spiroplasma citri and the corn stunt Spiroplasma.

    abstract::Components of membranes isolated from Spiroplasma citri and corn stunt spiroplasma grown at 28 degrees C were analyzed. On a protein basis, lipid phosphorus was lower and cholesterol was higher in S. citri. Only minor differences between the two species were found in fatty acid composition, reduced nicotinamide adenin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.137.2.1056-1058.1979

    authors: Mudd JB,Lee IM,Liu HY,Calavan EC

    更新日期:1979-02-01 00:00:00

  • Alpha-l-arabinofuranosidase from Rhodotorula flava.

    abstract::An alpha-L-arabinofuranosidase (EC 3.2.1.55) from the culture fluid of Rhodotorula flava IFO 0407 grown on beet arabinan as a carbon source has been highly purified. The purified enzyme has a pH optimum of 2.0. The enzyme is unusually acid stable, retaining 82% of its activity after being maintained for 24 h at pH 1.5...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.133.3.1073-1077.1978

    authors: Uesaka E,Sato M,Raiju M,Kaji A

    更新日期:1978-03-01 00:00:00

  • Regulation of fatty acid metabolism by FadR is essential for Vibrio vulnificus to cause infection of mice.

    abstract::The opportunistic bacterial pathogen Vibrio vulnificus causes severe wound infection and fatal septicemia. We used alkaline phosphatase insertion mutagenesis in a clinical isolate of V. vulnificus to find genes necessary for virulence, and we identified fadR, which encodes a regulator of fatty acid metabolism. The fad...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01016-08

    authors: Brown RN,Gulig PA

    更新日期:2008-12-01 00:00:00

  • Functional interactions between coexisting toxin-antitoxin systems of the ccd family in Escherichia coli O157:H7.

    abstract::Toxin-antitoxin (TA) systems are widely represented on mobile genetic elements as well as in bacterial chromosomes. TA systems encode a toxin and an antitoxin neutralizing it. We have characterized a homolog of the ccd TA system of the F plasmid (ccd(F)) located in the chromosomal backbone of the pathogenic O157:H7 Es...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01679-06

    authors: Wilbaux M,Mine N,Guérout AM,Mazel D,Van Melderen L

    更新日期:2007-04-01 00:00:00

  • Purification and properties of mutant and wild-type diaphorases from Diplococcus pneumoniae.

    abstract::Optochin-resistant mutant and wild-type diaphorases were purified approximately 300-fold by a combination of batch adsorption and column chromatography with diethylaminoethyl cellulose, and were characterized with regard to their pH optima, sensitivity to optochin inhibition and heat inactivation, Michaelis constants ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.4.1029-1036.1968

    authors: Kreger AS,Olsen RH

    更新日期:1968-10-01 00:00:00

  • Delineation of the caffeine C-8 oxidation pathway in Pseudomonas sp. strain CBB1 via characterization of a new trimethyluric acid monooxygenase and genes involved in trimethyluric acid metabolism.

    abstract::The molecular basis of the ability of bacteria to live on caffeine via the C-8 oxidation pathway is unknown. The first step of this pathway, caffeine to trimethyluric acid (TMU), has been attributed to poorly characterized caffeine oxidases and a novel quinone-dependent caffeine dehydrogenase. Here, we report the deta...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00597-12

    authors: Mohanty SK,Yu CL,Das S,Louie TM,Gakhar L,Subramanian M

    更新日期:2012-08-01 00:00:00

  • Replication of the nonconjugative plasmid RSF1010 in Escherichia coli K-12.

    abstract::Replicating DNA molecules of the nonconjugative R plasmid RSF1010 (Smr Sur) were cleaved with the EcoRI restriction endonuclease and examined with the electron microscope. Results of this analysis indicated that replication is initiated from an origin located at about 19% of total genome size from one of the EcoRI end...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.134.3.1117-1122.1978

    authors: de Graaff J,Crosa JH,Heffron F,Falkow S

    更新日期:1978-06-01 00:00:00

  • The penicillinase of Bacillus licheniformis is an outer membrane protein in Escherichia coli.

    abstract::The cloned gene coding for Bacillus licheniformis penicillinase (penP) was introduced into Escherichia coli in a heat-inducible lambda Qam vector. After induction, significant amounts of penicillinase were synthesized in the new host. The cellular location of the penicillinase was found to be almost exclusively the ou...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.155.2.657-663.1983

    authors: Sarvas MO,Palva IA

    更新日期:1983-08-01 00:00:00

  • Transcriptional induction of an Agrobacterium regulatory gene at tandem promoters by plant-released phenolic compounds, phosphate starvation, and acidic growth media.

    abstract::Transcription of the virG gene of Agrobacterium tumefaciens was previously shown to be expressed from two tandem promoters and to be responsive to three stimuli: plant-released phenolic compounds, phosphate starvation, and acidic media. In this report, I describe a set of deletions and other alterations of the 5' end ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.5.2433-2438.1990

    authors: Winans SC

    更新日期:1990-05-01 00:00:00

  • Characterization of a novel Rieske-type alkane monooxygenase system in Pusillimonas sp. strain T7-7.

    abstract::The cold-tolerant bacterium Pusillimonas sp. strain T7-7 is able to utilize diesel oils (C5 to C30 alkanes) as a sole carbon and energy source. In the present study, bioinformatics, proteomics, and real-time reverse transcriptase PCR approaches were used to identify the alkane hydroxylation system present in this bact...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02107-12

    authors: Li P,Wang L,Feng L

    更新日期:2013-05-01 00:00:00