Dynamics of FtsZ assembly during sporulation in Streptomyces coelicolor A3(2).

Abstract:

:FtsZ, the bacterial tubulin homologue, is the main player in at least two distinct processes of cell division during the development of Streptomyces coelicolor A3(2). It forms cytokinetic rings and is required for the formation of both the widely spaced hyphal cross walls in the substrate mycelium and the specialized septation that converts sporogenic aerial hyphae into spores. The latter developmentally controlled septation involves the coordinated assembly of large numbers of FtsZ rings in each sporulating hyphal cell. We used an FtsZ-enhanced green fluorescent protein (EGFP) translational fusion to visualize the progression of FtsZ ring assembly in vivo during sporulation of aerial hyphae. This revealed that the regular placement of multiple FtsZ rings and initiation of cytokinesis was preceded by a protracted phase during which spiral-shaped FtsZ intermediates were detected along the length of the aerial hyphal cell. Time course experiments indicated that they were remodeled and gradually replaced by regularly spaced FtsZ rings. Such spiral-shaped filaments could also be detected with immunofluorescence microscopy using an antiserum against FtsZ. Based on our observations, we propose a model for the progression of Z-ring assembly during sporulation of S. coelicolor. Furthermore, mutants lacking the developmental regulatory genes whiA, whiB, whiG, whiH, and whiI were investigated. They failed in up-regulation of the expression of FtsZ-EGFP in aerial hyphae, which is consistent with the known effects of these genes on ftsZ transcription.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Grantcharova N,Lustig U,Flärdh K

doi

10.1128/JB.187.9.3227-3237.2005

keywords:

subject

Has Abstract

pub_date

2005-05-01 00:00:00

pages

3227-37

issue

9

eissn

0021-9193

issn

1098-5530

pii

187/9/3227

journal_volume

187

pub_type

杂志文章
  • Rhizobium meliloti mutants unable to synthesize anthranilate display a novel symbiotic phenotype.

    abstract::Analyses of Rhizobium meliloti trp auxotrophs suggest that anthranilate biosynthesis by the R. meliloti trpE(G) gene product is necessary during nodule development for establishment of an effective symbiosis. trpE(G) mutants, as well as mutants blocked earlier along this pathway in aromatic amino acid biosynthesis, fo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.13.4416-4426.1992

    authors: Barsomian GD,Urzainqui A,Lohman K,Walker GC

    更新日期:1992-07-01 00:00:00

  • High-frequency RecA-dependent and -independent mechanisms of Congo red binding mutations in Yersinia pestis.

    abstract::Yersinia pestis, which causes bubonic and pneumonic plague, forms pigmented red colonies on Congo red (CR) dye agar. The hmsHFRS genes required for CR binding (Crb(+)) are genetically linked to virulence-associated genes encoding a siderophore uptake system. These genes are contained in a 102-kb chromosomal pgm locus ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.16.4896-4904.1999

    authors: Hare JM,McDonough KA

    更新日期:1999-08-01 00:00:00

  • Identification by genetic suppression of Escherichia coli TolB residues important for TolB-Pal interaction.

    abstract::The Tol-Pal system of Escherichia coli is involved in maintaining outer membrane stability. Mutations in tolQ, tolR, tolA, tolB, or pal genes result in sensitivity to bile salts and the leakage of periplasmic proteins. Moreover, some of the tol genes are necessary for the entry of group A colicins and the DNA of filam...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.3.821-824.2000

    authors: Ray MC,Germon P,Vianney A,Portalier R,Lazzaroni JC

    更新日期:2000-02-01 00:00:00

  • The proton motive force generated in Leuconostoc oenos by L-malate fermentation.

    abstract::In cells of Leuconostoc oenos, the fermentation of L-malic acid generates both a transmembrane pH gradient, inside alkaline, and an electrical potential gradient, inside negative. In resting cells, the proton motive force ranged from -170 mV to -88 mV between pH 3.1 and 5.6 in the presence Of L-malate. Membrane potent...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.11.3127-3132.1996

    authors: Salema M,Lolkema JS,San Romão MV,Lourero Dias MC

    更新日期:1996-06-01 00:00:00

  • Glycosylation is required for outer membrane localization of the lectin LecB in Pseudomonas aeruginosa.

    abstract::The fucose-/mannose-specific lectin LecB from Pseudomonas aeruginosa is transported to the outer membrane; however, the mechanism used is not known so far. Here, we report that LecB is present in the periplasm of P. aeruginosa in two variants of different sizes. Both were functional and could be purified by their affi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01507-10

    authors: Bartels KM,Funken H,Knapp A,Brocker M,Bott M,Wilhelm S,Jaeger KE,Rosenau F

    更新日期:2011-03-01 00:00:00

  • Factors regulating competence in transformation of streptococci.

    abstract::Pakula, Roman (University of Toronto, Toronto, Ontario, Canada). Factors regulating competence in transformation of streptococci. J. Bacteriol. 90:1320-1324. 1965.-The highly transformable group H Streptococcus strain Challis produced an exocellular competence-provoking enzyme capable of converting to the state of com...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.5.1320-1324.1965

    authors: Pakula R

    更新日期:1965-11-01 00:00:00

  • Inhibitory effect of diphtheria toxin on amino acid incorporation in Escherichia coli cell-free system.

    abstract::The mechanism of action of diphtheria toxin in an Escherichia coli cell-free protein-synthesizing system was examined. When the washed ribosomes were incubated with toxin before addition of messenger ribonucleic acid (RNA), peptide syntheses of (14)C-phenylalanine directed by polyuridylic acid or phage R17 RNA were st...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.1.152-157.1970

    authors: Tsugawa A,Ohsumi Y,Kato I

    更新日期:1970-10-01 00:00:00

  • Identification of phosphate starvation-inducible genes in Escherichia coli K-12 by DNA sequence analysis of psi::lacZ(Mu d1) transcriptional fusions.

    abstract::Twenty-four independent phosphate starvation-inducible (psi) transcriptional fusions made with Mu d1(lacZbla) were analyzed by sequencing the psi::lacZ(Mu d1) chromosomal junctions by using DNAs amplified with the polymerase chain reaction or mini-Mu cloning. Our DNA sequence analysis showed that the MuR DNA in Mu d1 ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.6.3191-3200.1990

    authors: Metcalf WW,Steed PM,Wanner BL

    更新日期:1990-06-01 00:00:00

  • Draft genome sequence of probiotic strain Lactobacillus rhamnosus R0011.

    abstract::Lactobacillus rhamnosus R0011 is a commercially available probiotic that is widely used in human dietary supplements and pharmaceutical products. We prepared a draft genome sequence consisting of 10 contigs totaling 2,900,620 bases and a G+C content of 46.7% for this strain. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06584-11

    authors: Tompkins TA,Barreau G,de Carvalho VG

    更新日期:2012-02-01 00:00:00

  • Genome-wide analyses in bacteria show small-RNA enrichment for long and conserved intergenic regions.

    abstract::Interest in finding small RNAs (sRNAs) in bacteria has significantly increased in recent years due to their regulatory functions. Development of high-throughput methods and more sophisticated computational algorithms has allowed rapid identification of sRNA candidates in different species. However, given their various...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02359-14

    authors: Tsai CH,Liao R,Chou B,Palumbo M,Contreras LM

    更新日期:2015-01-01 00:00:00

  • Properties of adenyl cyclase and cyclic adenosine 3',5'-monophosphate receptor protein-deficient mutants of Escherichia coli.

    abstract::Several spontaneous cya and crp mutants of Escherichia coli have been selected as clones simultaneously resistant to phage lambda and nalidixic acid and characterized. Both cya and crp mutants have been found to grow as cocci with increased doubling times. They have increased resistance to some mutagens (methylmethane...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.125.2.545-555.1976

    authors: Kumar S

    更新日期:1976-02-01 00:00:00

  • Diversity of Tn4001 transposition products: the flanking IS256 elements can form tandem dimers and IS circles.

    abstract::We show that both flanking IS256 elements carried by transposon Tn4001 are capable of generating head-to-tail tandem copies and free circular forms, implying that both are active. Our results suggest that the tandem structures arise from dimeric copies of the donor or vector plasmid present in the population by a mech...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.2.433-443.2002

    authors: Prudhomme M,Turlan C,Claverys JP,Chandler M

    更新日期:2002-01-01 00:00:00

  • Acetylation of the response regulator RcsB controls transcription from a small RNA promoter.

    abstract::Nε-lysine acetylation was recently discovered on many bacterial proteins that function in diverse cellular processes. Thus, many questions remain unanswered. For example, what mechanisms regulate lysine acetylation? Does acetylation affect physiology? To help answer these questions, we studied the Escherichia coli res...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00383-13

    authors: Hu LI,Chi BK,Kuhn ML,Filippova EV,Walker-Peddakotla AJ,Bäsell K,Becher D,Anderson WF,Antelmann H,Wolfe AJ

    更新日期:2013-09-01 00:00:00

  • Ultrastructure of Treponema microdentium and Borrelia vincentii.

    abstract::Bladen, Howard A. (National Institute of Dental Health, Bethesda, Md.), and Edward G. Hampp. Ultrastructure of Treponema microdentium and Borrelia vincentii. J. Bacteriol. 87:1180-1191.-A small oral Treponema (FM) and Borrelia vincentii (N9) were harvested after 3 to 7 days of incubation and either embedded in Vestopa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.5.1180-1191.1964

    authors: Bladen HA,Hampp EG

    更新日期:1964-05-01 00:00:00

  • Expression of a Saccharomyces cerevisiae photolyase gene in Escherichia coli.

    abstract::A 3.3-kilobase PvuII fragment carrying the PHR1 gene of Saccharomyces cerevisiae has been cloned into an Escherichia coli expression vector and introduced into E. coli strains deficient in DNA photolyase. Complementation of the E. coli phr-1 mutation was observed, strongly suggesting that the yeast PHR1 gene encodes a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.161.2.769-771.1985

    authors: Sancar GB

    更新日期:1985-02-01 00:00:00

  • Bacterial calcium transport: energy-dependent calcium uptake by membrane vesicles from Bacillus megaterium.

    abstract::Membrane vesicles capable of energy-dependent calcium uptake have been prepared from Bacillus megaterium cells in log-phase growth or when undergoing sporulation. The uptake is dependent on the calcium concentration and appears saturable in vesicles from cells in log-phase growth. Both ascorbate and phenazine methosul...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.119.3.840-843.1974

    authors: Golub EE,Bronner F

    更新日期:1974-09-01 00:00:00

  • Lon Protease Removes Excess Signal Recognition Particle Protein in Escherichia coli.

    abstract::Correct targeting of membrane proteins is essential for membrane integrity, cell physiology, and viability. Cotranslational targeting depends on the universally conserved signal recognition particle (SRP), which is a ribonucleoprotein complex comprised of the protein component Ffh and the 4.5S RNA in Escherichia coli ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00161-20

    authors: Sauerbrei B,Arends J,Schünemann D,Narberhaus F

    更新日期:2020-06-25 00:00:00

  • Pleiotropic effect of a histidine kinase on carbohydrate metabolism in Synechocystis sp. strain PCC 6803 and its requirement for heterotrophic growth.

    abstract::The deletion of a gene coding for a histidine kinase (sll0750, Hik8) in the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 resulted in a conditional lethal phenotype with a pleiotropic effect on the expression of genes involved in glucose metabolism. This mutant had comparable doubling times to wild type...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.7.2368-2376.2005

    authors: Singh AK,Sherman LA

    更新日期:2005-04-01 00:00:00

  • A DNA region recognized by the nitric oxide-responsive transcriptional activator NorR is conserved in beta- and gamma-proteobacteria.

    abstract::The sigma(54)-dependent regulator NorR activates transcription of target genes in response to nitric oxide (NO) or NO-generating agents. In Ralstonia eutropha H16, NorR activates transcription of the dicistronic norAB operon that encodes NorA, a protein of unknown function, and NorB, a nitric oxide reductase. A consti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.23.7980-7987.2004

    authors: Büsch A,Pohlmann A,Friedrich B,Cramm R

    更新日期:2004-12-01 00:00:00

  • Cloning and characterization of the gene for a protein thiol-disulfide oxidoreductase in Bacillus brevis.

    abstract::The gene (bdb) for protein thiol-disulfide oxidoreductase cloned from Bacillus brevis was found to encode a polypeptide consisting of 117 amino acid residues with a signal peptide of 27 residues. Bdb contains a well-conserved motif, Cys-X-X-Cys, which functions as the active center of disulfide oxidoreductases such as...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.3.745-749.1995

    authors: Ishihara T,Tomita H,Hasegawa Y,Tsukagoshi N,Yamagata H,Udaka S

    更新日期:1995-02-01 00:00:00

  • Formation of protoporphyrin from coproporphyrinogen in extracts of various bacteria.

    abstract::The enzyme system capable of converting coproporphyrinogen to protoporphyrin was demonstrated in the soluble fraction of extracts of Pseudomonas fluorescens grown aerobically, of P. denitrificans grown anaerobically under denitrifying conditions, and of Escherichia coli grown both aerobically and anaerobically. Protop...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.102.2.398-403.1970

    authors: Jacobs NJ,Jacobs JM,Brent P

    更新日期:1970-05-01 00:00:00

  • Copurification of glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridyltransferase activities of Escherichia coli: characterization of the glmU gene product as a bifunctional enzyme catalyzing two subsequent steps in the pat

    abstract::The glmU gene product of Escherichia coli was recently identified as the N-acetylglucosamine-1-phosphate uridyltransferase activity which catalyzes the formation of UDP-N-acetylglucosamine, an essential precursor for cell wall peptidoglycan and lipopolysaccharide biosyntheses (D. Mengin-Lecreulx and J. van Heijenoort,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.18.5788-5795.1994

    authors: Mengin-Lecreulx D,van Heijenoort J

    更新日期:1994-09-01 00:00:00

  • Transformation and transfection of Pseudomonas aeruginosa: effects of metal ions.

    abstract::The ability of different metal ions to promote transformation of Pseudomonas aeruginosa by deoxyribonucleic acid of the plasmid RP1 was examined. CaCl2, MgCl2, and MnCl2 were found to promote such transformation, although at different frequencies and with the optimum response at different concentrations. Only MgCl2 pr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.1.37-42.1979

    authors: Mercer AA,Loutit JS

    更新日期:1979-10-01 00:00:00

  • Manganese, superoxide dismutase, and oxygen tolerance in some lactic acid bacteria.

    abstract::A previous study of the aerotolerant bacterium Lactobacillus plantarum, which lacks superoxide dismutase (SOD), demonstrated that it possesses a novel substitute for this defensive enzyme. Thus, L. plantarum contains 20 to 25 mM Mn(II),m in a dialyzable form, which is able to scavenge O2- apparently as effectively as ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.146.3.928-936.1981

    authors: Archibald FS,Fridovich I

    更新日期:1981-06-01 00:00:00

  • Characterization of two methanopterin biosynthesis mutants of Methylobacterium extorquens AM1 by use of a tetrahydromethanopterin bioassay.

    abstract::An enzymatic assay was developed to measure tetrahydromethanopterin (H(4)MPT) levels in wild-type and mutant cells of Methylobacterium extorquens AM1. H(4)MPT was detectable in wild-type cells but not in strains with a mutation of either the orf4 or the dmrA gene, suggesting a role for these two genes in H(4)MPT biosy...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.5.1565-1570.2004

    authors: Rasche ME,Havemann SA,Rosenzvaig M

    更新日期:2004-03-01 00:00:00

  • METHICILLIN-INDUCED LYSOZYME-SENSITIVE FORMS OF STAPHYLOCOCCI.

    abstract::Aldrich, K. M. (The University of Kansas, Lawrence), and C. P. Sword. Methicillin-induced lysozyme-sensitive forms of staphylococci. J. Bacteriol. 87:690-695. 1964.-Staphylococcus aureus and S. epidermidis grown in the presence of sublethal amounts of methicillin were converted to enlarged spheres within 2 to 4 hr, as...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.3.690-695.1964

    authors: ALDRICH KM,SWORD CP

    更新日期:1964-03-01 00:00:00

  • Molecular characterization of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824.

    abstract::A gene (aad) coding for an aldehyde/alcohol dehydrogenase (AAD) was identified immediately upstream of the previously cloned ctfA (J. W. Cary, D. J. Petersen, E. T. Papoutsakis, and G. N. Bennett, Appl. Environ. Microbiol. 56:1576-1583, 1990) of Clostridium acetobutylicum ATCC 824 and sequenced. The 2,619-bp aad codes...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.3.871-885.1994

    authors: Nair RV,Bennett GN,Papoutsakis ET

    更新日期:1994-02-01 00:00:00

  • cse15, cse60, and csk22 are new members of mother-cell-specific sporulation regulons in Bacillus subtilis.

    abstract::We report on the characterization of three new transcription units expressed during sporulation in Bacillus subtilis. Two of the units, cse15 and cse60, were mapped at about 123 degrees and 62 degrees on the genetic map, respectively. Their transcription commenced around h 2 of sporulation and showed an absolute requi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.2.389-398.1997

    authors: Henriques AO,Bryan EM,Beall BW,Moran CP Jr

    更新日期:1997-01-01 00:00:00

  • Escherichia coli produces linoleic acid during late stationary phase.

    abstract::Escherichia coli produces linoleic acid in the late stationary phase. This was the case whether the cultures were grown aerobically or anaerobically on a supplemented glucose-salts medium. The linoleic acid was detected by thin-layer chromatography and was measured as the methyl ester by gas chromatography. The linole...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.17.5324-5328.1993

    authors: Rabinowitch HD,Sklan D,Chace DH,Stevens RD,Fridovich I

    更新日期:1993-09-01 00:00:00

  • Cloning, sequence analysis, and functional expression of the acetyl coenzyme A synthetase gene from Methanothrix soehngenii in Escherichia coli.

    abstract::In the acetoclastic methanogen Methanothrix soehngenii, acetate is activated to acetyl coenzyme A by acetyl coenzyme A synthetase (Acs). The acs gene, coding for the single Acs subunit, was isolated from a genomic library of M. soehngenii DNA in Escherichia coli by using antiserum raised against the purified Acs. Afte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.20.6383-6389.1991

    authors: Eggen RI,Geerling AC,Boshoven AB,de Vos WM

    更新日期:1991-10-01 00:00:00