Abstract:
:In cells of Leuconostoc oenos, the fermentation of L-malic acid generates both a transmembrane pH gradient, inside alkaline, and an electrical potential gradient, inside negative. In resting cells, the proton motive force ranged from -170 mV to -88 mV between pH 3.1 and 5.6 in the presence Of L-malate. Membrane potentials were calculated by using a model for probe binding that accounted for the different binding constants at the different pH values at the two faces of the membrane. The delta psi generated by the transport of monovalent malate, H-malate-, controlled the rate of fermentation. The fermentation rate significantly increased under conditions of decreased delta psi, i.e., upon addition of the ionophore valinomycin in the presence of KCl, whereas in a buffer depleted of potassium, the addition of valinomycin resulted in a hyperpolarization of the cell membrane and a reduction of the rate of fermentation. At the steady state, the chemical gradient for H-malate- was of the same magnitude as delta psi. Synthesis of ATP was observed in cells performing malolactic fermentation.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Salema M,Lolkema JS,San Romão MV,Lourero Dias MCdoi
10.1128/jb.178.11.3127-3132.1996subject
Has Abstractpub_date
1996-06-01 00:00:00pages
3127-32issue
11eissn
0021-9193issn
1098-5530journal_volume
178pub_type
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doi:10.1128/JB.98.3.1342-1354.1969
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abstract::Proline-requiring mutants of Saccharomyces cerevisiae were isolated. Each mutation is recessive and is inherited as expected for a single nuclear gene. Three complementation groups cold be defined which are believed to correspond to mutations in the three genes (pro1, pro2, and pro3) coding for the three enzymes of th...
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pub_type: 杂志文章
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更新日期:1979-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1978-02-01 00:00:00
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更新日期:2009-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1995-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.105.3.1119-1136.1971
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更新日期:1998-12-01 00:00:00
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更新日期:1979-04-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2009-10-01 00:00:00
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更新日期:2003-07-01 00:00:00
abstract::Two isozymes of chorismate mutase (CA mutase(1) and CA mutase(2)) and two isozymes of prephenate dehydratase (PPA dehydratase(1) and PPA dehydratase(2)) have been found in Pseudomonas aeruginosa. The activities CA mutase(2)-PPA dehydratase(2) catalyzing phenylalanine biosynthesis have been purified almost 40-fold and ...
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doi:10.1128/JB.115.1.205-212.1973
更新日期:1973-07-01 00:00:00