Cellular autolytic activity in synchronized populations of Streptococcus faecium.

Abstract:

:The autolytic capacity of Streptococcus faecium (S. faecalis ATCC 9790) varied during synchronous cell division. This phenomenon was initially observed in rapidly dividing populations (TD=30 to 33 min) synchronized by a combination of induction and size selection techniques. To minimize the problems inherent in studies of cells containing overlapping chromosome cycles and possible artifacts generated by induction techniques, the autolytic capacities of slowly dividing populations (TD=60 to 110 min) synchronized by selection only were examined. Although the overall level of cellular autolytic capacity was observed to decline with decreasing growth rate, sharp, periodic fluctuations in cellular autolytic capacity were seen during synchronous growth at all growth rates examined. On the basis of similar patterns of cyclic fluctuations in autolytic capacity of cultures synchronized by (i) selection, (ii) amino acid starvation followed by size selection, and (iii) amino acid starvation followed by inhibition of DNA synthesis, a link of such fluctuations with the cell division cycle has been postulated.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Hinks RP,Daneo-Moore L,Shockman GD

doi

10.1128/JB.133.2.822-829.1978

subject

Has Abstract

pub_date

1978-02-01 00:00:00

pages

822-9

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

133

pub_type

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