Transposition of Tn4551 in Bacteroides fragilis: identification and properties of a new transposon from Bacteroides spp.

abstract：:The Rhizobium phaseoli recA gene has been cloned by interspecific complementation of the Fec phenotype of bacteriophage lambda. The cloned gene restored the recombination proficiency and conferred resistance to DNA-damaging agents (methyl methanesulfonate and nitrofurantoin) to an Escherichia coli recA mutant. The dir...

journal_title：Journal of bacteriology

authors： Martínez-Salazar JM,Romero D,Girard ML,Dávila G

更新日期：1991-05-01 00:00:00

abstract：:We examined in detail conditions necessary for making reproducible and for maximizing the amount of deoxyribonucleic acid obtained from a sex factor-containing cell as covalent circles. The results argue that under optimal conditions covalent circles are neither created nor lost during the isolation procedure. The cau...

journal_title：Journal of bacteriology

authors： Freifelder D,Folkmanis A,Kirschner I

更新日期：1971-03-01 00:00:00

abstract：:Copper is both a required micronutrient and a source of toxicity in most organisms, including Campylobacter jejuni Two proteins expressed in C. jejuni (termed CopA and CueO) have been shown to be a copper transporter and multicopper oxidase, respectively. We have isolated strains with mutations in these genes, and her...

journal_title：Journal of bacteriology

authors： Gardner SP,Olson JW

更新日期：2018-10-10 00:00:00

abstract：:The PmrA/PmrB two-component system encoded by the pmrCAB operon regulates the modification of Salmonella enterica serovar Typhimurium lipopolysaccharide leading to polymyxin B resistance. PmrA and PhoP are the only known activators of pmrCAB. A transposon mutagenesis screen for additional regulators of a pmrC::MudJ fu...

journal_title：Journal of bacteriology

authors： Merighi M,Carroll-Portillo A,Septer AN,Bhatiya A,Gunn JS

更新日期：2006-01-01 00:00:00

abstract：:In Escherichia coli, the CpxRA two-component signal transduction system senses and responds to aggregated and misfolded proteins in the bacterial envelope. We show that CpxR-P (the phosphorylated form of the cognate response regulator) activates cpxRA expression in conjunction with RpoS, suggesting an involvement of t...

journal_title：Journal of bacteriology

authors： De Wulf P,Kwon O,Lin EC

更新日期：1999-11-01 00:00:00

abstract：:Woody-Karrer, Pearl (Palo Alto Medical Research Foundation, Palo Alto, Calif.) and Joseph Greenberg. Resistance and cross resistance of Escherichia coli S mutants to the radiomimetic agent nitrofurazone. J. Bacteriol. 85:1208-1216. 1963.-Cross-resistance relationships are described for 73 mutants of Escherichia coli s...

journal_title：Journal of bacteriology

authors： WOODY-KARRER P,GREENBERG J

更新日期：1963-06-01 00:00:00

abstract：:Sulfolobus sulfataricus ATCC 35091, Haloferax volcanii, and Methanosarcina thermophila TM-1, representing the Euryarchaeota and Crenarchaeota subdomains of the Archaea, contain proteins which are phosphorylated on tyrosine. These data raise fundamental questions as to the origin and evolution of tyrosine phosphorylati...

journal_title：Journal of bacteriology

authors： Smith SC,Kennelly PJ,Potts M

更新日期：1997-04-01 00:00:00

abstract：:Antiserum was made against a single 30S protein, 30S-7. The amount of complement fixed by total 30S protein in the presence of this antiserum indicated that protein 30S-7 was the major antigen in the mixture of proteins. Each of the 30S ribosomal proteins was tested for cross-reactivity with anti-30S-7. This was done ...

journal_title：Journal of bacteriology

authors： Strnad BC,Sypherd PS

更新日期：1971-11-01 00:00:00

abstract：:The upper operon of the TOL plasmid pWW0 of Pseudomonas putida encodes a set of enzymes which transform toluene and xylenes to benzoate and toluates. The genetic organization of the operon was characterized by cloning of the upper operon genes into an expression vector and identification of their products in Escherich...

journal_title：Journal of bacteriology

authors： Harayama S,Rekik M,Wubbolts M,Rose K,Leppik RA,Timmis KN

更新日期：1989-09-01 00:00:00

abstract：:Information on the biosynthesis of phospholipids in bacteria has been derived principally from the study of Escherichia coli and other gram-negative organisms. We have now carried out a detailed study of the pathways of phospholipid biosynthesis in the gram-positive organism Bacillus megarterium KM in relation to inve...

journal_title：Journal of bacteriology

authors： Langley KE,Yaffe MP,Kennedy EP

更新日期：1979-12-01 00:00:00

abstract：:When carbon sources become limiting for growth, bacteria must choose which of the remaining nutrients should be used first. We have identified a nutrient-sensing signaling network in Escherichia coli that is activated at the transition to stationary phase. The network is composed of the two histidine kinase/response r...

journal_title：Journal of bacteriology

authors： Behr S,Fried L,Jung K

更新日期：2014-06-01 00:00:00

abstract：:Chloroethylthiamine was found to inhibit an entrapment of thiamine as thiamine monophosphate by blocking thiamine monophosphokinase in the cytoplasm after thiamine was taken up by the cells of Escherichia coli. ...

journal_title：Journal of bacteriology

authors： Iwashima A,Nose Y

更新日期：1972-12-01 00:00:00

abstract：:The photosynthetic bacterium Rhodobacter capsulatus contains two [NiFe]hydrogenases: an energy-generating hydrogenase, HupSL, and a regulatory hydrogenase, HupUV. The synthesis of HupSL is specifically activated by H(2) through a signal transduction cascade comprising three proteins: the H(2)-sensing HupUV protein, th...

journal_title：Journal of bacteriology

authors： Elsen S,Duché O,Colbeau A

更新日期：2003-12-01 00:00:00

abstract：:The tprK gene of Treponema pallidum subsp. pallidum, the causative agent of venereal syphilis, belongs to a 12-member gene family and encodes a protein with a predicted cleavable signal sequence and predicted transmembrane domains. Except for the Nichols type strain, all rabbit-propagated isolates of T. pallidum exami...

journal_title：Journal of bacteriology

authors： LaFond RE,Centurion-Lara A,Godornes C,Rompalo AM,Van Voorhis WC,Lukehart SA

更新日期：2003-11-01 00:00:00

abstract：:Heterocyst differentiation is orchestrated by the N control transcriptional regulator NtcA and the differentiation-specific factor HetR. In Anabaena sp. strain PCC 7120, the devBCA operon is expressed from two different promoters activated upon N stepdown. The distal devB promoter (transcription start point [TSP] loca...

journal_title：Journal of bacteriology

authors： Camargo S,Valladares A,Flores E,Herrero A

更新日期：2012-06-01 00:00:00

abstract：:Physiological studies of a mutant of Escherichia coli lacking the three lytic transglycosylases Slt70, MltA, and MltB revealed that interference with murein turnover can prevent AmpC beta-lactamase induction. The triple mutant, although growing normally, shows a dramatically reduced rate of murein turnover. Despite th...

journal_title：Journal of bacteriology

authors： Kraft AR,Prabhu J,Ursinus A,Höltje JV

更新日期：1999-12-01 00:00:00

abstract：:Gaffar, Abdul (Brigham Young University, Provo, Utah), David R. Terry, and Richard D. Sagers. Amino acid composition of walls from single and filamentous cells of Clostridium acidiurici. J. Bacteriol. 91:1618-1624. 1966.-The walls from single and filamentous cells of Clostridium acidiurici were shown to contain 11 ami...

journal_title：Journal of bacteriology

authors： Gaffar A,Terry DR,Sagers RD

更新日期：1966-04-01 00:00:00

abstract：:The gene specifying a membrane-bound nucleoside diphosphate sugar hydrolase of Salmonella typhimurium was mapped near the metA locus by using intergeneric crosses between this bacterium and Escherichia coli. ...

journal_title：Journal of bacteriology

authors： Minton N,Gunn J,Beacham IR

更新日期：1979-03-01 00:00:00

abstract：:We have isolated a hybrid plasmid, pDB(mei2)2, containing a 7.4-kilobases (kb) DNA fragment from a Schizosaccharomyces pombe genomic library which is able to complement the mei2 mutation of S. pombe. Integration of the cloned DNA sequence at the mei2 site on chromosome I demonstrated that it contained the mei2 gene. T...

journal_title：Journal of bacteriology

authors： Shimoda C,Uehira M,Kishida M,Fujioka H,Iino Y,Watanabe Y,Yamamoto M

更新日期：1987-01-01 00:00:00

abstract：:Poly(U)- and poly(UG)-programmed cell-free systems were developed from the extreme thermophilic, anaerobic eubacterium Thermotoga maritima, and their susceptibility to aminoglycoside and other antibiotics was assayed at a temperature (75 degrees C) close to the physiological optimum (80 degrees C) for cell growth and ...

journal_title：Journal of bacteriology

authors： Londei P,Altamura S,Huber R,Stetter KO,Cammarano P

更新日期：1988-09-01 00:00:00

abstract：:Amino acid transport rates and amino acid binding proteins were examined in a strain containing the rho-120 mutation (formerly SuA), which has been shown to lower the rho-dependent, ribonucleic acid-activated adenosine triphosphatase activity to 9% of the rho activity in the isogenic wild-type strain. Tryptophan and p...

journal_title：Journal of bacteriology

authors： Quay SC,Oxender DL

更新日期：1977-06-01 00:00:00

abstract：:The ultimate membrane localization and function of most of the 185 predicted Pseudomonas aeruginosa PAO1 lipoproteins remain unknown. We constructed a fluorescent lipoprotein, CSFP(OmlA)-ChFP, by fusing the signal peptide and the first four amino acids of the P. aeruginosa outer membrane lipoprotein OmlA to the monome...

journal_title：Journal of bacteriology

authors： Lewenza S,Mhlanga MM,Pugsley AP

更新日期：2008-09-01 00:00:00

abstract：:The Bacillus subtilis PurR mediates adenine repression and guanosine induction of purA. PRPP inhibits binding of PurR to DNA in vitro. Mutations in the PRPP binding motif of PurR caused strong repression regardless of purine exclusions or additions, establishing the role of PRPP as regulator of PurR. ...

journal_title：Journal of bacteriology

authors： Rappu P,Pullinen T,Mäntsälä P

更新日期：2003-11-01 00:00:00

abstract：:The Escherichia coli LytB protein regulates the activity of guanosine 3',5'-bispyrophosphate synthetase I (RelA). A Southern blot analysis of chromosomal DNA with the E. coli lytB gene as a probe revealed the presence of lytB homologs in all of the gram-negative bacterial species examined but not in gram-positive spec...

journal_title：Journal of bacteriology

authors： Potter S,Yang X,Boulanger MJ,Ishiguro EE

更新日期：1998-04-01 00:00:00

abstract：:Biofilm formation in Staphylococcus epidermidis is dependent upon the ica operon-encoded polysaccharide intercellular adhesin, which is subject to phase-variable and environmental regulation. The icaR gene, located adjacent to the ica operon, appears to be a member of the tetR family of transcriptional regulators. In ...

journal_title：Journal of bacteriology

authors： Conlon KM,Humphreys H,O'Gara JP

更新日期：2002-08-01 00:00:00

abstract：:The endophytic diazotroph Gluconacetobacter diazotrophicus secretes a constitutively expressed levansucrase (LsdA, EC 2.4.1.10) to utilize plant sucrose. LsdA, unlike other extracellular levansucrases from gram-negative bacteria, is transported to the periplasm by a signal-peptide-dependent pathway. We identified an u...

journal_title：Journal of bacteriology

authors： Arrieta JG,Sotolongo M,Menéndez C,Alfonso D,Trujillo LE,Soto M,Ramírez R,Hernández L

更新日期：2004-08-01 00:00:00

abstract：:A Rhodobacter sphaeroides mutant (CYCA1) lacking cytochrome c2 (cyt c2) was previously constructed (T. J. Donohue, A. G. McEwan, S. Van Doren, A. R. Crofts, and S. Kaplan, Biochemistry, 27: 1918-1924, 1988) by a combination of in vivo and in vitro molecular genetic techniques. CYCA1 was incapable of photosynthetic gro...

journal_title：Journal of bacteriology

authors： Brandner JP,McEwan AG,Kaplan S,Donohue TJ

更新日期：1989-01-01 00:00:00

abstract：:Pseudomonas syringae injects numerous bacterial proteins into host plant cells through a type 3 secretion system (T3SS). One of the first such bacterial effectors discovered, HopA1, is a protein that has unknown functions in the host cell but possesses close homologs that trigger the plant hypersensitive response in r...

journal_title：Journal of bacteriology

authors： Janjusevic R,Quezada CM,Small J,Stebbins CE

更新日期：2013-02-01 00:00:00

abstract：:Listeria monocytogenes is a gram-positive, psychotrophic, food-borne pathogen which is able to grow in osmotically stressful environments. Carnitine (beta-hydroxy-L-tau-N-trimethyl aminobutyrate) can contribute significantly to growth of L. monocytogenes at high osmolarity (R. R. Beumer, M. C. te Giffel, L. J. Cox, F....

journal_title：Journal of bacteriology

authors： Verheul A,Rombouts FM,Beumer RR,Abee T

更新日期：1995-06-01 00:00:00

abstract：:Purified suspensions of Chlamydia psittaci were prepared from L cells. Thin sections of intact elementary bodies and intact developmental reticulate bodies and of their purified envelopes were observed by electron microscopy. In both intact organisms and partially purified envelopes, two membranous structures, each ap...

journal_title：Journal of bacteriology

authors： Tamura A,Matsumoto A,Manire GP,Higashi N

更新日期：1971-01-01 00:00:00