Cloning and analysis of transcription of the mei2 gene responsible for initiation of meiosis in the fission yeast Schizosaccharomyces pombe.


:We have isolated a hybrid plasmid, pDB(mei2)2, containing a 7.4-kilobases (kb) DNA fragment from a Schizosaccharomyces pombe genomic library which is able to complement the mei2 mutation of S. pombe. Integration of the cloned DNA sequence at the mei2 site on chromosome I demonstrated that it contained the mei2 gene. This gene was localized on a 4.7-kb HindIII-PvuII fragment in the subclone pFMV402. Transcriptional regulation was studied by Northern blot analysis in which polyadenylated RNA was prepared from a heterozygous (h+N/h-S) diploid strain cultured either in nitrogen-rich growth medium or in nitrogen-free sporulation medium. The size of the major mei2 mRNA, which always gave a broad band, was estimated to be 4.2 +/- 0.2 kb, and a few minor bands (e.g., 3.2 and 1.8 kb) appeared as well. These transcripts appeared more abundantly in sporulating cells than in growing cells. Neither the mating type genes (mat) nor the mei3 gene was essential for transcription of the mei2 gene, since ample mei2 mRNA was detected in sporulation-deficient cells transferred to sporulation medium, such as h+N/h+N and h-S/h-S homozygotes, as well as mei1 and mei3 mutants.


J Bacteriol


Journal of bacteriology


Shimoda C,Uehira M,Kishida M,Fujioka H,Iino Y,Watanabe Y,Yamamoto M




Has Abstract


1987-01-01 00:00:00












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    journal_title:Journal of bacteriology

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    authors: Stubbs JD,Stubbs EA

    更新日期:1971-12-01 00:00:00


    abstract::Deibel, R. H. (American Meat Institute Foundation, Chicago, Ill.). Hydrolysis of proteins and nucleic acids by Lancefield group A and other streptococci. J. Bacteriol. 86:1270-1274. 1963.-Classically, group A streptococci have been considered to be nonproteolytic in spite of the observation that a proteinase is produc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: DEIBEL RH

    更新日期:1963-12-01 00:00:00

  • Crystalline inclusions of bacterium 22M.

    abstract::Crystalline inclusions occur in bacterium 22M. Arrays consist of parallel rows of polyhedral subunits (7.5 nm in diameter), 2.0 nm apart, with a distance of 3.0 nm between rows. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kim KS,Barksdale L

    更新日期:1969-06-01 00:00:00

  • Carrier-mediated transport of folate in a mutant of Pediococcus cerevisiae.

    abstract::A mutant strain of Pediococcus cerevisiae (P. cerevisiae/PteGlu) was isolated which grows on low-folate (PteGlu) concentrations (200 pg/ml). The growth response of the parent and mutant strains to folinate (5-CHO-H(4)PteGlu) was the same. The transport of (14)C-PteGlu by P. cerevisiae/PteGlu was temperature-dependent ...

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    authors: Mandelbaum-Shavit F,Grossowicz N

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  • Isolation of a high-affinity functional protein complex between OmcA and MtrC: Two outer membrane decaheme c-type cytochromes of Shewanella oneidensis MR-1.

    abstract::Shewanella oneidensis MR-1 is a facultatively anaerobic bacterium capable of using soluble and insoluble forms of manganese [Mn(III/IV)] and iron [Fe(III)] as terminal electron acceptors during anaerobic respiration. To assess the structural association of two outer membrane-associated c-type decaheme cytochromes (i.e...

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    abstract::The 2,760-base-pair (bp) PstI-EcoRI segment of the chromosome of Pseudomonas aeruginosa PA103 which carries the exotoxin A structural gene was expressed from an internal promoter when cloned in a pUC9 derivative and transformed into a nontoxigenic mutant of P. aeruginosa PAO1. The unique terminal EcoRI site was delete...

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    journal_title:Journal of bacteriology

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    authors: Hurek T,Egener T,Reinhold-Hurek B

    更新日期:1997-07-01 00:00:00

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    abstract::Supercoiled double-stranded DNA molecules (plasmids) were isolated from plants infected with three laboratory strains of western aster yellows mycoplasma-like organism (AY-MLO) by using cesium chloride-ethidium bromide density gradients. Southern blot analysis, using plasmids from the severe strain of AY-MLO (SAY-MLO)...

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    pub_type: 杂志文章


    authors: Pierson BK,Keith LM,Leovy JG

    更新日期:1984-07-01 00:00:00

  • Chromosomal replication origins (oriC) of Enterobacter aerogenes and Klebsiella pneumoniae are functional in Escherichia coli.

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    authors: Calcutt MJ,Cundliffe E

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    journal_title:Journal of bacteriology

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    authors: Marty-Teysset C,Lolkema JS,Schmitt P,Diviès C,Konings WN

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  • The cell envelope structure of the lipopolysaccharide-lacking gram-negative bacterium Sphingomonas paucimobilis.

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    pub_type: 杂志文章


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    更新日期:1994-01-01 00:00:00

  • Asp2 and Asp3 interact directly with GspB, the export substrate of the Streptococcus gordonii accessory Sec System.

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  • Global gene expression in Staphylococcus aureus biofilms.

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    authors: Beenken KE,Dunman PM,McAleese F,Macapagal D,Murphy E,Projan SJ,Blevins JS,Smeltzer MS

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    abstract::Gilden, R. V. (Wistar Institute, Philadelphia, Pa.), and R. I. Carp. Effects of cycloheximide and puromycin on synthesis of simian virus 40 T antigen in green monkey kidney cells. J. Bacteriol. 91:1295-1297. 1966.-Synthesis of the simian virus 40 (SV40) T antigen in primary African green monkey kidney cells was abolis...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Gilden RV,Carp RI

    更新日期:1966-03-01 00:00:00

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    abstract::A simple three-step procedure is described which yields electrophoretically homogeneous preparations of ribitol-5-phosphate dehydrogenase and xylitol-5-phosphate dehydrogenase. The former enzyme is a 115,000-molecular-weight protein composed of two subunits of identical size and is specific for its substrate, ribitol....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hausman SZ,London J

    更新日期:1987-04-01 00:00:00

  • Characterization of the Azorhizobium sesbaniae ORS571 genomic locus encoding NADPH-glutamate synthase.

    abstract::Sixteen independent Azorhizobium sesbaniae ORS571 vector insertion (Vi) mutants defective in ammonium assimilation (Asm-) were selected; genomic DNA sequences flanking the insertion endpoints were cloned directly. Resulting recombinant plasmids were used to identify, by hybridization, corresponding wild-type DNA seque...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Donald RG,Lapointe J,Ludwig RA

    更新日期:1988-03-01 00:00:00

  • Flavobacterium johnsoniae gldN and gldO are partially redundant genes required for gliding motility and surface localization of SprB.

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Rhodes RG,Samarasam MN,Shrivastava A,van Baaren JM,Pochiraju S,Bollampalli S,McBride MJ

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Yan LP,Hitchins AD

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Poole K,Schiebel E,Braun V

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  • A Mutation in the Bacillus subtilis rsbU Gene That Limits RNA Synthesis during Sporulation.

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Pauli G,Ehring R,Overath P

    更新日期:1974-03-01 00:00:00

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Wang X,Tao F,Gai Z,Tang H,Xu P

    更新日期:2012-11-01 00:00:00

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Pokholok DK,Redlak M,Turnbough CL Jr,Dylla S,Holmes WM

    更新日期:1999-09-01 00:00:00

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Mattern SG,Brawner ME,Westpheling J

    更新日期:1993-03-01 00:00:00