Cloning and analysis of transcription of the mei2 gene responsible for initiation of meiosis in the fission yeast Schizosaccharomyces pombe.

Abstract:

:We have isolated a hybrid plasmid, pDB(mei2)2, containing a 7.4-kilobases (kb) DNA fragment from a Schizosaccharomyces pombe genomic library which is able to complement the mei2 mutation of S. pombe. Integration of the cloned DNA sequence at the mei2 site on chromosome I demonstrated that it contained the mei2 gene. This gene was localized on a 4.7-kb HindIII-PvuII fragment in the subclone pFMV402. Transcriptional regulation was studied by Northern blot analysis in which polyadenylated RNA was prepared from a heterozygous (h+N/h-S) diploid strain cultured either in nitrogen-rich growth medium or in nitrogen-free sporulation medium. The size of the major mei2 mRNA, which always gave a broad band, was estimated to be 4.2 +/- 0.2 kb, and a few minor bands (e.g., 3.2 and 1.8 kb) appeared as well. These transcripts appeared more abundantly in sporulating cells than in growing cells. Neither the mating type genes (mat) nor the mei3 gene was essential for transcription of the mei2 gene, since ample mei2 mRNA was detected in sporulation-deficient cells transferred to sporulation medium, such as h+N/h+N and h-S/h-S homozygotes, as well as mei1 and mei3 mutants.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Shimoda C,Uehira M,Kishida M,Fujioka H,Iino Y,Watanabe Y,Yamamoto M

doi

10.1128/jb.169.1.93-96.1987

subject

Has Abstract

pub_date

1987-01-01 00:00:00

pages

93-6

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

169

pub_type

杂志文章
  • Influences of various amino acids on tryptophan-mediated control of the tryptophan biosynthetic enzymes in Escherichia coli.

    abstract::Lysates of Escherichia coli Ymel obtained from cultures grown in the absence of tryptophan in minimal medium supplemented with 0.1% casein hydrolysate show an approximate fivefold increase in steady-state specific activity of both anthranilate synthetase and tryptophan synthetase A protein relative to cultures grown i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.108.3.1181-1191.1971

    authors: Stubbs JD,Stubbs EA

    更新日期:1971-12-01 00:00:00

  • HYDROLYSIS OF PROTEINS AND NUCLEIC ACIDS BY LANCEFIELD GROUP A AND OTHER STREPTOCOCCI.

    abstract::Deibel, R. H. (American Meat Institute Foundation, Chicago, Ill.). Hydrolysis of proteins and nucleic acids by Lancefield group A and other streptococci. J. Bacteriol. 86:1270-1274. 1963.-Classically, group A streptococci have been considered to be nonproteolytic in spite of the observation that a proteinase is produc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.86.6.1270-1274.1963

    authors: DEIBEL RH

    更新日期:1963-12-01 00:00:00

  • Crystalline inclusions of bacterium 22M.

    abstract::Crystalline inclusions occur in bacterium 22M. Arrays consist of parallel rows of polyhedral subunits (7.5 nm in diameter), 2.0 nm apart, with a distance of 3.0 nm between rows. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.3.1390-1394.1969

    authors: Kim KS,Barksdale L

    更新日期:1969-06-01 00:00:00

  • Carrier-mediated transport of folate in a mutant of Pediococcus cerevisiae.

    abstract::A mutant strain of Pediococcus cerevisiae (P. cerevisiae/PteGlu) was isolated which grows on low-folate (PteGlu) concentrations (200 pg/ml). The growth response of the parent and mutant strains to folinate (5-CHO-H(4)PteGlu) was the same. The transport of (14)C-PteGlu by P. cerevisiae/PteGlu was temperature-dependent ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.114.2.485-490.1973

    authors: Mandelbaum-Shavit F,Grossowicz N

    更新日期:1973-05-01 00:00:00

  • Isolation of a high-affinity functional protein complex between OmcA and MtrC: Two outer membrane decaheme c-type cytochromes of Shewanella oneidensis MR-1.

    abstract::Shewanella oneidensis MR-1 is a facultatively anaerobic bacterium capable of using soluble and insoluble forms of manganese [Mn(III/IV)] and iron [Fe(III)] as terminal electron acceptors during anaerobic respiration. To assess the structural association of two outer membrane-associated c-type decaheme cytochromes (i.e...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01966-05

    authors: Shi L,Chen B,Wang Z,Elias DA,Mayer MU,Gorby YA,Ni S,Lower BH,Kennedy DW,Wunschel DS,Mottaz HM,Marshall MJ,Hill EA,Beliaev AS,Zachara JM,Fredrickson JK,Squier TC

    更新日期:2006-07-01 00:00:00

  • Localization of the control region for expression of exotoxin A in Pseudomonas aeruginosa.

    abstract::The 2,760-base-pair (bp) PstI-EcoRI segment of the chromosome of Pseudomonas aeruginosa PA103 which carries the exotoxin A structural gene was expressed from an internal promoter when cloned in a pUC9 derivative and transformed into a nontoxigenic mutant of P. aeruginosa PAO1. The unique terminal EcoRI site was delete...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.5.2599-2604.1989

    authors: Tsaur ML,Clowes RC

    更新日期:1989-05-01 00:00:00

  • Divergence in nitrogenases of Azoarcus spp., Proteobacteria of the beta subclass.

    abstract::Nitrogenase is a functionally constant protein catalyzing N2 reduction, which is found in many phylogenetic lineages of Archaea and Bacteria. A phylogenetic analysis of nif genes may provide insights into the evolution of the bacterial genomes. Moreover, it may be used to study diazotrophic communities, when classical...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.13.4172-4178.1997

    authors: Hurek T,Egener T,Reinhold-Hurek B

    更新日期:1997-07-01 00:00:00

  • Identification and characterization of plasmids from the western aster yellows mycoplasmalike organism.

    abstract::Supercoiled double-stranded DNA molecules (plasmids) were isolated from plants infected with three laboratory strains of western aster yellows mycoplasma-like organism (AY-MLO) by using cesium chloride-ethidium bromide density gradients. Southern blot analysis, using plasmids from the severe strain of AY-MLO (SAY-MLO)...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.3.1628-1633.1990

    authors: Kuske CR,Kirkpatrick BC

    更新日期:1990-03-01 00:00:00

  • Isolation of pigmentation mutants of the green filamentous photosynthetic bacterium Chloroflexus aurantiacus.

    abstract::Mutants deficient in the production of bacteriochlorophyll c (Bchl c) and one mutant lacking colored carotenoids were isolated from the filamentous gliding bacterium Chloroflexus aurantiacus. Mutagenesis was achieved by using UV radiation or N-methyl-N'-nitro-N-nitrosoguanidine. Several clones were isolated that were ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.159.1.222-227.1984

    authors: Pierson BK,Keith LM,Leovy JG

    更新日期:1984-07-01 00:00:00

  • Chromosomal replication origins (oriC) of Enterobacter aerogenes and Klebsiella pneumoniae are functional in Escherichia coli.

    abstract::The chromosomal DNA replication origins (oriC) from two members of the family Enterobacteriaceae, Enterobacter aerogenes and Klebsiella pneumoniae, have been isolated as functional replication origins in Escherichia coli. The origins in the SalI restriction fragments of 17.5 and 10.2 kilobase pairs, cloned from E. aer...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Harding NE,Cleary JM,Smith DW,Michon JJ,Brusilow WS,Zyskind JW

    更新日期:1982-12-01 00:00:00

  • Cloning of a lincosamide resistance determinant from Streptomyces caelestis, the producer of celesticetin, and characterization of the resistance mechanism.

    abstract::Self-resistance has been investigated in Streptomyces caelestis (producer of the lincosamide antibiotic celesticetin), from which a lincosamide resistance determinant (clr) has been isolated on a 1-kilobase DNA fragment and cloned in Streptomyces lividans. The clr product is a specific methylase which produces a singl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.8.4710-4714.1990

    authors: Calcutt MJ,Cundliffe E

    更新日期:1990-08-01 00:00:00

  • The citrate metabolic pathway in Leuconostoc mesenteroides: expression, amino acid synthesis, and alpha-ketocarboxylate transport.

    abstract::Citrate metabolism in Leuconostoc mesenteroides subspecies mesenteroides is associated with the generation of a proton motive force by a secondary mechanism (C. Marty-Teysset, C. Posthuma, J. S. Lolkema, P. Schmitt, C. Divies, and W. N. Konings, J. Bacteriol. 178:2178-2185, 1996). The pathway consists of four steps: (...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.21.6209-6215.1996

    authors: Marty-Teysset C,Lolkema JS,Schmitt P,Diviès C,Konings WN

    更新日期:1996-11-01 00:00:00

  • The cell envelope structure of the lipopolysaccharide-lacking gram-negative bacterium Sphingomonas paucimobilis.

    abstract::From the cell envelope preparation of Sphingomonas paucimobilis two membrane fractions with different densities were separated by sucrose density gradient ultracentrifugation. The high-density fraction contained several major proteins, phospholipids, and glycosphingolipids, which are the only glycolipids of this lipop...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.2.284-290.1994

    authors: Kawasaki S,Moriguchi R,Sekiya K,Nakai T,Ono E,Kume K,Kawahara K

    更新日期:1994-01-01 00:00:00

  • Asp2 and Asp3 interact directly with GspB, the export substrate of the Streptococcus gordonii accessory Sec System.

    abstract::GspB is a serine-rich glycoprotein adhesin of Streptococcus gordonii that is exported to the bacterial surface by the accessory Sec system. This dedicated export pathway is comprised of seven components (SecA2, SecY2, and five accessory Sec proteins [Asp1 to Asp5]). The latter proteins have no known homologs beyond th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00057-11

    authors: Yen YT,Seepersaud R,Bensing BA,Sullam PM

    更新日期:2011-07-01 00:00:00

  • Chemoreceptors and flagellar motors are subterminally located in close proximity at the two cell poles in spirochetes.

    abstract::Green fluorescent protein (GFP) fusions, immunofluorescence microscopy, and cryo-electron tomography revealed that the chemoreceptors of the Lyme disease spirochete Borrelia burgdorferi form long, thin arrays near both cell poles. These arrays are in close proximity to the flagellar motors. This information provides a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01530-10

    authors: Xu H,Raddi G,Liu J,Charon NW,Li C

    更新日期:2011-05-01 00:00:00

  • Global gene expression in Staphylococcus aureus biofilms.

    abstract::We previously demonstrated that mutation of the staphylococcal accessory regulator (sarA) in a clinical isolate of Staphylococcus aureus (UAMS-1) results in an impaired capacity to form a biofilm in vitro (K. E. Beenken, J. S. Blevins, and M. S. Smeltzer, Infect. Immun. 71:4206-4211, 2003). In this report, we used a m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.14.4665-4684.2004

    authors: Beenken KE,Dunman PM,McAleese F,Macapagal D,Murphy E,Projan SJ,Blevins JS,Smeltzer MS

    更新日期:2004-07-01 00:00:00

  • Effects of cycloheximide and puromycin on synthesis of simian virus 40 T antigen in green monkey kidney cells.

    abstract::Gilden, R. V. (Wistar Institute, Philadelphia, Pa.), and R. I. Carp. Effects of cycloheximide and puromycin on synthesis of simian virus 40 T antigen in green monkey kidney cells. J. Bacteriol. 91:1295-1297. 1966.-Synthesis of the simian virus 40 (SV40) T antigen in primary African green monkey kidney cells was abolis...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.3.1295-1297.1966

    authors: Gilden RV,Carp RI

    更新日期:1966-03-01 00:00:00

  • Purification and characterization of ribitol-5-phosphate and xylitol-5-phosphate dehydrogenases from strains of Lactobacillus casei.

    abstract::A simple three-step procedure is described which yields electrophoretically homogeneous preparations of ribitol-5-phosphate dehydrogenase and xylitol-5-phosphate dehydrogenase. The former enzyme is a 115,000-molecular-weight protein composed of two subunits of identical size and is specific for its substrate, ribitol....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.4.1651-1655.1987

    authors: Hausman SZ,London J

    更新日期:1987-04-01 00:00:00

  • Characterization of the Azorhizobium sesbaniae ORS571 genomic locus encoding NADPH-glutamate synthase.

    abstract::Sixteen independent Azorhizobium sesbaniae ORS571 vector insertion (Vi) mutants defective in ammonium assimilation (Asm-) were selected; genomic DNA sequences flanking the insertion endpoints were cloned directly. Resulting recombinant plasmids were used to identify, by hybridization, corresponding wild-type DNA seque...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.3.1197-1204.1988

    authors: Donald RG,Lapointe J,Ludwig RA

    更新日期:1988-03-01 00:00:00

  • Flavobacterium johnsoniae gldN and gldO are partially redundant genes required for gliding motility and surface localization of SprB.

    abstract::Cells of the gliding bacterium Flavobacterium johnsoniae move rapidly over surfaces. Mutations in gldN cause a partial defect in gliding. A novel bacteriophage selection strategy was used to aid construction of a strain with a deletion spanning gldN and the closely related gene gldO in an otherwise wild-type F. johnso...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01495-09

    authors: Rhodes RG,Samarasam MN,Shrivastava A,van Baaren JM,Pochiraju S,Bollampalli S,McBride MJ

    更新日期:2010-03-01 00:00:00

  • Comparative macromolecular composition of filaments and rods of a Bacillus megaterium thermoconditional morphological mutant.

    abstract::Filaments of a thermosensitive Bacillus megaterium mutant showed an altered macromolecular composition compared with salt-cured mutant cells and parental cells. Filaments contained more peptidoglycan, polyglucose, poly-beta-hydroxy-butyrate, and deoxyribonucleic acid per unit of protein. The ribonucleic acid-to-protei...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.144.1.454-456.1980

    authors: Yan LP,Hitchins AD

    更新日期:1980-10-01 00:00:00

  • Isolation and preliminary characterization of Saccharomyces cerevisiae proline auxotrophs.

    abstract::Proline-requiring mutants of Saccharomyces cerevisiae were isolated. Each mutation is recessive and is inherited as expected for a single nuclear gene. Three complementation groups cold be defined which are believed to correspond to mutations in the three genes (pro1, pro2, and pro3) coding for the three enzymes of th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.138.3.816-822.1979

    authors: Brandriss MC

    更新日期:1979-06-01 00:00:00

  • Swarming: flexible roaming plans.

    abstract::Movement over an agar surface via swarming motility is subject to formidable challenges not encountered during swimming. Bacteria display a great deal of flexibility in coping with these challenges, which include attracting water to the surface, overcoming frictional forces, and reducing surface tension. Bacteria that...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,评审

    doi:10.1128/JB.02063-12

    authors: Partridge JD,Harshey RM

    更新日期:2013-03-01 00:00:00

  • Molecular characterization of the hemolysin determinant of Serratia marcescens.

    abstract::The nucleotide sequence of a 7.3-kilobase-pair fragment of DNA encoding a hemolytic activity from Serratia marcescens was determined. Two large open reading frames were identified, designated shlA (Serratia hemolysin) and shlB, capable of encoding polypeptides of 165, 056 and 61,897 molecular weight, respectively. Bot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.7.3177-3188.1988

    authors: Poole K,Schiebel E,Braun V

    更新日期:1988-07-01 00:00:00

  • A Mutation in the Bacillus subtilis rsbU Gene That Limits RNA Synthesis during Sporulation.

    abstract::Mutants of Bacillis subtilis that are temperature sensitive for RNA synthesis during sporulation were isolated after selection with a 32P suicide agent. Whole-genome sequencing revealed that two of the mutants carried an identical lesion in the rsbU gene, which encodes a phosphatase that indirectly activates SigB, the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00212-17

    authors: Rothstein DM,Lazinski D,Osburne MS,Sonenshein AL

    更新日期:2017-06-27 00:00:00

  • Formation of molecular complexes between a structurally defined M protein and acylated or deacylated lipoteichoic acid of Streptococcus pyogenes.

    abstract::The orientation of lipoteichoic acid (LTA) molecules on the surface of bacterial cells undoubtedly is determined by the ability of the LTA, during its transit through the cell wall, to bind via its polyglycerophosphate backbone or its glycolipid moieties to other constituents of the cytoplasmic membrane and the cell w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.149.2.426-433.1982

    authors: Ofek I,Simpson WA,Beachey EH

    更新日期:1982-02-01 00:00:00

  • Fatty acid degradation in Escherichia coli: requirement of cyclic adenosine monophosphate and cyclic adenosine monophosphate receptor protein for enzyme synthesis.

    abstract::The strong repression of inducible synthesis of the enzymes of fatty acid degradation by glucose can be partially relieved by the addition of cyclic adenosine 3',5' monophosphate (cyclic AMP) to the growth medium. This reversal of the glucose effect by cyclic AMP is not observed in a mutant (K29) that is unable to gro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.117.3.1178-1183.1974

    authors: Pauli G,Ehring R,Overath P

    更新日期:1974-03-01 00:00:00

  • Genome sequence of the welan gum-producing strain Sphingomonas sp. ATCC 31555.

    abstract::Sphingomonas sp. strain ATCC 31555 can produce an anionic heteropolysaccharide, welan gum, which shows excellent stability and viscosity retention even at high temperatures. Here we present a 4.0-Mb assembly of its genome sequence. We have annotated 10 coding sequences (CDSs) responsible for the welan gum biosynthesis...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01486-12

    authors: Wang X,Tao F,Gai Z,Tang H,Xu P

    更新日期:2012-11-01 00:00:00

  • Multiple mechanisms are used for growth rate and stringent control of leuV transcriptional initiation in Escherichia coli.

    abstract::Expression of the Escherichia coli leuV operon, which contains three tRNA(1)(Leu) genes, is regulated by several mechanisms including growth-rate-dependent control (GRDC) and stringent control (SC). Structural variants of the leuV promoter which differentially affect these regulatory responses have been identified, su...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.18.5771-5782.1999

    authors: Pokholok DK,Redlak M,Turnbough CL Jr,Dylla S,Holmes WM

    更新日期:1999-09-01 00:00:00

  • Identification of a complex operator for galP1, the glucose-sensitive, galactose-dependent promoter of the Streptomyces galactose operon.

    abstract::The galP1 promoter is responsible for galactose-dependent, glucose-sensitive transcription of the galactose utilization operon of Streptomyces coelicolor and Streptomyces lividans. We describe the characterization of mutations that were positioned directly upstream of the apparent transcription start site of galP1 and...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.5.1213-1220.1993

    authors: Mattern SG,Brawner ME,Westpheling J

    更新日期:1993-03-01 00:00:00