Highly efficient protoplast transformation system for Streptococcus faecalis and a new Escherichia coli-S. faecalis shuttle vector.

abstract：:The disaccharide trehalose is accumulated as a storage product by spores of Streptomyces griseus. Nongerminating spores used their trehalose reserves slowly when incubated in buffer for several months. In contrast, spores rapidly depleted their trehalose pools during the first hours of germination. Extracts of dormant...

journal_title：Journal of bacteriology

authors： McBride MJ,Ensign JC

更新日期：1987-11-01 00:00:00

abstract：:Johnson, Russell C. (University of Minnesota, Minneapolis), and Palmer Rogers. Differentiation of pathogenic and saprophytic leptospires with 8-azaguanine. J. Bacteriol. 88:1618-1623. 1964.-The use of the purine analogue, 8-azaguanine, as a differential agent for the separation of pathogenic and saprophytic leptospire...

journal_title：Journal of bacteriology

authors： JOHNSON RC,ROGERS P

更新日期：1964-12-01 00:00:00

abstract：:The first generalized transducing bacteriophage reported for Bacillus megaterium has been characterized. Optimum conditions for lysate production and transduction procedures were established so that transducing frequencies of 8 x 10(-6) and higher are now possible. The phage, MP13, has a head diameter of 97 nm and a c...

journal_title：Journal of bacteriology

authors： Vary PS,Garbe JC,Franzen M,Frampton EW

更新日期：1982-03-01 00:00:00

abstract：:The glucosamine-1-phosphate acetyltransferase activity but not the uridyltransferase activity of the bifunctional GlmU enzyme from Escherichia coli was lost when GlmU was stored in the absence of beta-mercaptoethanol or incubated with thiol-specific reagents. The enzyme was protected from inactivation in the presence ...

journal_title：Journal of bacteriology

authors： Pompeo F,van Heijenoort J,Mengin-Lecreulx D

更新日期：1998-09-01 00:00:00

abstract：:Burkholderia cepacia AC1100 metabolizes 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) via formation of 5-chlorohydroxyquinol (5-CHQ), hydroxyquinol (HQ), maleylacetate, and beta-oxoadipate. The step(s) leading to the dechlorination of 5-CHQ to HQ has remained unidentified. We demonstrate that a dechlorinating enzyme, Tf...

journal_title：Journal of bacteriology

authors： Zaborina O,Daubaras DL,Zago A,Xun L,Saido K,Klem T,Nikolic D,Chakrabarty AM

更新日期：1998-09-01 00:00:00

abstract：:The tar and tap loci of Escherichia coli encode methyl-accepting inner membrane proteins that mediate chemotactic responses to aspartate and maltose or to dipeptides. These genes lie adjacent to each other in the same orientation on the chromosome and have extensive sequence homology throughout the C-terminal portions...

journal_title：Journal of bacteriology

authors： Slocum MK,Halden NF,Parkinson JS

更新日期：1987-07-01 00:00:00

abstract：:The physiological changes in an F1-ATPase-defective mutant of Escherichia coli W1485 growing in a glucose-limited chemostat included a decreased growth yield (60%) and increased specific rates of both glucose consumption (168%) and respiration (171%). Flux analysis revealed that the mutant showed approximately twice a...

journal_title：Journal of bacteriology

authors： Noda S,Takezawa Y,Mizutani T,Asakura T,Nishiumi E,Onoe K,Wada M,Tomita F,Matsushita K,Yokota A

更新日期：2006-10-01 00:00:00

abstract：:The nucleotide sequence of a region located downstream of the nifB gene, both in the cyanobacterium Anabaena sp. strain PCC 7120 and in Rhizobium meliloti, has been determined. This region contains a gene (fdxN) whose predicted polypeptide product strongly resembles typical bacterial ferredoxins. Cyanobacteria have no...

journal_title：Journal of bacteriology

authors： Mulligan ME,Buikema WJ,Haselkorn R

更新日期：1988-09-01 00:00:00

abstract：:The Ssa subfamily of Hsp70 molecular chaperones in the budding yeast Saccharomyces cerevisiae has four members, encoded by SSA1, SSA2, SSA3, and SSA4. Deletion of the two constitutively expressed genes, SSA1 and SSA2, results in cells which are slow growing and temperature sensitive. In this study, we demonstrate that...

journal_title：Journal of bacteriology

authors： Baxter BK,Craig EA

更新日期：1998-12-01 00:00:00

abstract：:Many DNA viruses concatemerize their genomes as a prerequisite to packaging into capsids. Concatemerization arises from either replication or homologous recombination. Replication is already the target of many antiviral drugs, and viral recombinases are an attractive target for drug design, particularly for combinatio...

journal_title：Journal of bacteriology

authors： Vellani TS,Myers RS

更新日期：2003-04-01 00:00:00

abstract：:The level of the vitamin B12 transport protein BtuB in the outer membrane of Escherichia coli is strongly reduced by growth in the presence of cobalamins. Previous analyses of regulatory mutants and of btuB-lacZ fusions indicated that the primary site of btuB gene regulation was at the translational level, and this re...

journal_title：Journal of bacteriology

authors： Nou X,Kadner RJ

更新日期：1998-12-01 00:00:00

abstract：:In vitro, archaeal SRP54 binds SRP RNA in the absence of SRP19, suggesting the latter to be expendable in Archaea. Accordingly, the Haloferax volcanii SRP19 gene was deleted. Although normally transcribed at a level comparable to that of the essential SRP54 gene, SRP19 deletion had no effect on cell growth, membrane p...

journal_title：Journal of bacteriology

authors： Yurist S,Dahan I,Eichler J

更新日期：2007-01-01 00:00:00

abstract：:A general, genetic technique for determining the direction of transcription for bacterial genes is presented. By comparing the phenotype of Mu-Mud1 dilysogens with the phenotype of deletion-containing derivatives, the direction of transcription for the gene containing Mud1 can be unambiguously determined. This method ...

journal_title：Journal of bacteriology

authors： MacNeil D

更新日期：1981-04-01 00:00:00

abstract：:Nontypeable Haemophilus influenzae (NTHi) is a gram-negative bacterium and a common commensal organism of the upper respiratory tract in humans. NTHi causes a number of diseases, including otitis media, sinusitis, conjunctivitis, exacerbations of chronic obstructive pulmonary disease, and bronchitis. During the course...

journal_title：Journal of bacteriology

authors： Harrison A,Ray WC,Baker BD,Armbruster DW,Bakaletz LO,Munson RS Jr

更新日期：2007-02-01 00:00:00

abstract：:In vitro deoxyribonucleic acid (DNA) synthesis systems based on an earlier system using pencillin have been developed which use osmotic lysis of lysozyme-formed spheroplasts of Escherichia coli cells embedded in an agarose matrix. An adenosine 5'-triphosphate (ATP)-dependent semiconservative mode, or replicative mode,...

journal_title：Journal of bacteriology

authors： Smith DW,Boerner P

更新日期：1975-04-01 00:00:00

abstract：:Weiss, Emilio (Naval Medical Research Institute, Bethesda, Md.) and Harry R. Dressler. Increased resistance to chloramphenicol in Rickettsia prowazekii with a note on failure to demonstrate genetic interaction among strains. J. Bacteriol. 83:409-414. 1962.-A further effort to produce genetically labeled Rickettsia pro...

journal_title：Journal of bacteriology

authors： WEISS E,DRESSLER HR

更新日期：1962-02-01 00:00:00

abstract：:Two types of adhesive fimbriae are expressed by Actinomyces; however, the architecture and the mechanism of assembly of these structures remain poorly understood. In this study we characterized two fimbrial gene clusters present in the genome of Actinomyces naeslundii strain MG-1. By using immunoelectron microscopy an...

journal_title：Journal of bacteriology

authors： Mishra A,Das A,Cisar JO,Ton-That H

更新日期：2007-04-01 00:00:00

abstract：:The Escherichia coli outer membrane protein Tsx functions as a nucleoside-specific channel and serves as the receptor for colicin K and a number of T-even-type bacteriophages, including phage T6. To identify those segments of the Tsx protein that are important for its phage receptor function, we devised a selection an...

journal_title：Journal of bacteriology

authors： Schneider H,Fsihi H,Kottwitz B,Mygind B,Bremer E

更新日期：1993-05-01 00:00:00

abstract：:Clostridium perfringens enterotoxin was detected intracellularly about 3 hr after the inoculation of vegetative cells into sporulation medium. The subsequent increase in intracellular enterotoxin concentration roughly paralleled but followed by 2.5 to 5 hr the increase in number of heat-resistant spores. The increase ...

journal_title：Journal of bacteriology

authors： Duncan CL

更新日期：1973-02-01 00:00:00

abstract：:The primary sequence of the small cysteine-rich protein (EnvA) of Chlamydia psittaci 6BC has been shown to possess a potential lipid modification/signal peptidase II-processing site, and the mature protein was labeled by a [3H]palmitic acid precursor. We further characterized the mature EnvA, showing that it lacks the...

journal_title：Journal of bacteriology

authors： Everett KD,Desiderio DM,Hatch TP

更新日期：1994-10-01 00:00:00

abstract：:Sinorhizobium meliloti is a free-living soil bacterium which is capable of establishing a symbiotic relationship with the alfalfa plant (Medicago sativa). This symbiosis involves a network of bacterium-host signaling, as well as the potential for bacterium-bacterium communication, such as quorum sensing. In this study...

journal_title：Journal of bacteriology

authors： Marketon MM,González JE

更新日期：2002-07-01 00:00:00

abstract：:Enzymes of the ATP-independent Deg serine endopeptidase family are very flexible with regard to their substrate specificity. Some family members cleave only one substrate, while others act as general proteases on unfolded substrates. The proteolytic activity of Deg proteases is regulated by PDZ protein interaction dom...

journal_title：Journal of bacteriology

authors： Huesgen PF,Scholz P,Adamska I

更新日期：2007-09-01 00:00:00

abstract：:An analysis of the turnover of the major phospholipids of Micrococcus cerificans growing or nongrowing cultures. The turnover rates of (14)C-PE and (14)C-PE were 61.5% of the total phospholipid, exhibited no significant rate of turnover in either growing or nongrowing cultures. The turnover rates of PE-(14)C and PE-(3...

journal_title：Journal of bacteriology

authors： Makula RA,Finnerty WR

更新日期：1971-09-01 00:00:00

abstract：:We have isolated the gene encoding L-allo-threonine aldolase (L-allo-TA) from Aeromonas jandaei DK-39, a pyridoxal 5'-phosphate (PLP)-dependent enzyme that stereospecifically catalyzes the interconversion of L-allo-threonine and glycine. The gene contains an open reading frame consisting of 1,014 nucleotides correspon...

journal_title：Journal of bacteriology

authors： Liu JQ,Dairi T,Kataoka M,Shimizu S,Yamada H

更新日期：1997-06-01 00:00:00

abstract：:The genetic transformation mutant Rd(DB117)rec- has a pleiotropic phenotype that includes reduced levels of phage recombination. Physical mapping experiments showed that this strain has a 78.5-kbp insertion in the rec-2 gene. The rec-2 dependence of phage recombination was reexamined to determine whether the defective...

journal_title：Journal of bacteriology

authors： Kupfer DM,McCarthy D

更新日期：1992-08-01 00:00:00

abstract：:The manganese-containing superoxide dismutase (MnSOD) of Vibrio vulnificus, normally detected after the onset of the stationary phase, is expressed during the lag that immediately follows the transfer of cells grown exponentially to a fresh medium acidified to pH 5.0, whereas Fe-containing SOD is constitutively expres...

journal_title：Journal of bacteriology

authors： Kim JS,Sung MH,Kho DH,Lee JK

更新日期：2005-09-01 00:00:00

abstract：:A variant of the diaminopimelate (DAP)-lysine biosynthesis pathway uses an LL-DAP aminotransferase (DapL, EC 2.6.1.83) to catalyze the direct conversion of L-2,3,4,5-tetrahydrodipicolinate to LL-DAP. Comparative genomic analysis and experimental verification of DapL candidates revealed the existence of two diverged fo...

journal_title：Journal of bacteriology

authors： Hudson AO,Gilvarg C,Leustek T

更新日期：2008-05-01 00:00:00

abstract：:Baker, F. D. (Western Reserve University, Cleveland, Ohio), H. R. Papiska, and L. Leon Campbell. Choline fermentation by Desulfovibrio desulfuricans. J. Bacteriol. 84:973-978. 1962-Hayward and Stadtman pointed out that the organism they described as Vibrio cholinicus is closely related to Desulfovibrio desulfuricans. ...

journal_title：Journal of bacteriology

authors： BAKER FD,PAPISKA HR,CAMPBELL LL

更新日期：1962-11-01 00:00:00

abstract：:Pseudomonas MS can grow on methylamine and a number of other compounds containing C1 units as a sole source of carbon and energy. Assimilation of carbon into cell material occurs via the "serine pathway" since enzymes of this pathway are induced after growth on methylamine, but not malate or acetate. A mutant has been...

journal_title：Journal of bacteriology

authors： Wagner C,Levitch ME

更新日期：1975-06-01 00:00:00

abstract：:Purified Rickettsia prowazekii cells were able to transport L-proline. The influx of this amino acid had a Kt of 14 microM and a Vmax of about 64 pmol/min per mg of protein. Proline could not be transported by heat-killed or metabolically poisoned rickettsiae or at 0 degrees C. The uptake of proline was linear for alm...

journal_title：Journal of bacteriology

authors： Winkler HH,Daugherty RM

更新日期：1984-05-01 00:00:00