Alterations of cellular physiology in Escherichia coli in response to oxidative phosphorylation impaired by defective F1-ATPase.

abstract：:Cell division of Escherichia coli K-12 strain PA3092 was inhibited by the addition of adenosine 3',5'-phosphate (cAMP), and the cellular morphology was changed from rods into filaments. Nucleoids in the filaments were regularly distributed and septum formation was perfectly inhibited. This inhibition of cell division ...

journal_title：Journal of bacteriology

authors： Utsumi R,Tanabe H,Nakamoto Y,Kawamukai M,Sakai H,Himeno M,Komano T,Hirota Y

更新日期：1981-09-01 00:00:00

abstract：:Glycogen accumulation in Escherichia coli is inversely related to the growth rate and occurs most actively when cells enter the stationary phase. The levels of the three biosynthetic enzymes undergo corresponding changes under these conditions, suggesting that genetic control of enzyme biosynthesis may account for at ...

journal_title：Journal of bacteriology

authors： Romeo T,Preiss J

更新日期：1989-05-01 00:00:00

abstract：:The R1162-encoded protein RepIB is essential for replication of the plasmid and binds specifically to iterons within the replicative origin. The protein causes the localized melting of DNA (determined by sensitivity to P1 nuclease) at a site within the AT-rich region of the origin, about 60 bp from the iteron binding ...

journal_title：Journal of bacteriology

authors： Kim YJ,Meyer RJ

更新日期：1991-09-01 00:00:00

abstract：:Halalkalicoccus jeotgali B3(T), isolated from salt-fermented seafood from South Korea, is an extremely halophilic archaeon belonging to the family Halobacteriaceae. Here, we present the complete genome sequence of the type strain H. jeotgali B3(T) (3,698,650 bp, with a G+C content of 62.5%), which consists of one chro...

journal_title：Journal of bacteriology

authors： Roh SW,Nam YD,Nam SH,Choi SH,Park HS,Bae JW

更新日期：2010-09-01 00:00:00

abstract：:The 50S ribosomal subunits obtained from exponential-phase and valine-limited cultures of Streptococcus faecalis 9790 differed in protein content. Approximately 35% of the valine-limited subunits showed protein losses when analyzed by CsCl density centrifugation after formaldehyde fixation. Results of density gradient...

journal_title：Journal of bacteriology

authors： Ziegler RJ,Daneo-Moore L

更新日期：1971-01-01 00:00:00

abstract：UNLABELLED:Over the course of the last 3 decades the role of the second messenger cyclic di-GMP (c-di-GMP) as a master regulator of bacterial physiology was determined. Although the control over c-di-GMP levels via synthesis and breakdown and the allosteric regulation of c-di-GMP over receptor proteins (effectors) and ...

journal_title：Journal of bacteriology

authors： Rotem O,Nesper J,Borovok I,Gorovits R,Kolot M,Pasternak Z,Shin I,Glatter T,Pietrokovski S,Jenal U,Jurkevitch E

更新日期：2015-08-31 00:00:00

abstract：:Proteins of the YidC/OxaI/Alb3 family play a crucial role in the insertion, folding, and/or assembly of membrane proteins in prokaryotes and eukaryotes. Bacillus subtilis has two YidC-like proteins, denoted SpoIIIJ and YqjG. SpoIIIJ and YqjG are largely exchangeable in function, but SpoIIIJ has a unique role in sporul...

journal_title：Journal of bacteriology

authors： Geng Y,de Keyzer J,Scheffers DJ,Driessen AJ

更新日期：2014-04-01 00:00:00

abstract：:The Tat system, found in the cytoplasmic membrane of many bacteria, is a general export pathway for folded proteins. Here we describe the development of a method, based on the transport of chloramphenicol acetyltransferase, that allows positive selection of mutants defective in Tat function. We have demonstrated the u...

journal_title：Journal of bacteriology

authors： Hicks MG,Lee PA,Georgiou G,Berks BC,Palmer T

更新日期：2005-04-01 00:00:00

abstract：:Sites of transposon Tn7 insertion in the Escherichia coli chromosome were examined, and two distinct classes of target sites differing in nucleotide sequence were identified. The target site choice was found to be determined by Tn7-encoded transposition genes. ...

journal_title：Journal of bacteriology

authors： Kubo KM,Craig NL

更新日期：1990-05-01 00:00:00

abstract：:The Rhodobacter capsulatus genome contains three genes (olsA [plsC138], plsC316, and plsC3498) that are annotated as lysophosphatidic acid (1-acyl-sn-glycerol-3-phosphate) acyltransferase (AGPAT). Of these genes, olsA was previously shown to be an O-acyltransferase in the second step of ornithine lipid biosynthesis, w...

journal_title：Journal of bacteriology

authors： Aygun-Sunar S,Bilaloglu R,Goldfine H,Daldal F

更新日期：2007-12-01 00:00:00

abstract：:Many genomes of anaerobic hyperthermophiles encode multiple homologs of NAD(P)H oxidase that are thought to function in response to oxidative stress. We investigated one of the seven NAD(P)H oxidase homologs (TK1481) in the sulfur-reducing hyperthermophilic archaeon Thermococcus kodakarensis, focusing on the catalytic...

journal_title：Journal of bacteriology

authors： Kobori H,Ogino M,Orita I,Nakamura S,Imanaka T,Fukui T

更新日期：2010-10-01 00:00:00

abstract：:We have developed a method, using laser, optical tweezers and direct microscopic analysis of reproductive potential and membrane integrity, to assess single-cell viability in a stationary-phase Escherichia coli population. It is demonstrated here that a reduction in cell integrity, determined by using the fluorescent ...

journal_title：Journal of bacteriology

authors： Ericsson M,Hanstorp D,Hagberg P,Enger J,Nyström T

更新日期：2000-10-01 00:00:00

abstract：:Type III secretion is used by many gram-negative bacterial pathogens to directly deliver protein toxins (effectors) into targeted host cells. In all cases, secretion of effectors is triggered by host cell contact, although the mechanism is unclear. In Pseudomonas aeruginosa, expression of all type III secretion-relate...

journal_title：Journal of bacteriology

authors： Cisz M,Lee PC,Rietsch A

更新日期：2008-04-01 00:00:00

abstract：:Concanavalin A-reactive linker and anchor subunits of phycobilisomes from Anacystis nidulans R2 (H. C. Riethman, T. P. Mawhinney, and L. A. Sherman, FEBS Lett. 215:209-214, 1987) were purified electrophoretically and analyzed for carbohydrate composition and quantity. Different quantities of glucose and N-acetylgalact...

journal_title：Journal of bacteriology

authors： Riethman HC,Mawhinney TP,Sherman LA

更新日期：1988-06-01 00:00:00

abstract：:Periplasmic nitrate reductase (NapABC enzyme) has been characterized from a variety of proteobacteria, especially Paracoccus pantotrophus. Whole-genome sequencing of Escherichia coli revealed the structural genes napFDAGHBC, which encode NapABC enzyme and associated electron transfer components. E. coli also expresses...

journal_title：Journal of bacteriology

authors： Stewart V,Lu Y,Darwin AJ

更新日期：2002-03-01 00:00:00

abstract：:Systematic inactivation of pathways involved in DNA alkylation damage repair demonstrated that inactivation of the ada, ogt, tag, uvrA, and mfd genes is required to detect a Salmonella enterica virulence decrease. Furthermore, the fitness of S. enterica, defective in these genes, is lowered only when the bacterium is ...

journal_title：Journal of bacteriology

authors： Alvarez G,Campoy S,Spricigo DA,Teixidó L,Cortés P,Barbé J

更新日期：2010-04-01 00:00:00

abstract：:The molecular mechanisms of regulation of the genes involved in the biosynthesis of cysteine are poorly characterized in Bacillus subtilis and other gram-positive bacteria. In this study we describe the expression pattern of the B. subtilis cysH operon in response to sulfur starvation. A 6.1-kb polycistronic transcrip...

journal_title：Journal of bacteriology

authors： Mansilla MC,Albanesi D,de Mendoza D

更新日期：2000-10-01 00:00:00

abstract：:The cysteine-rich envelope proteins of the elementary body form of chlamydiae are thought to be located in the outer membrane on the basis of their insolubility in the weak anionic detergent N-lauryl sarcosinate (Sarkosyl). We found, however, that the insolubility of the small (EnvA) and the large (EnvB) cysteine-rich...

journal_title：Journal of bacteriology

authors： Everett KD,Hatch TP

更新日期：1995-02-01 00:00:00

abstract：:The effect of a mutation in luxI (autoinducer synthetase gene) on transcription of luxR in the cloned Vibrio fischeri lux system (luxR, luxICDABE) was examined in Escherichia coli. For the luxI mutant, transcription from the luxR promoter (monitored with beta-galactosidase levels from a luxR::lacZ fusion, with LuxR su...

journal_title：Journal of bacteriology

authors： Dunlap PV,Ray JM

更新日期：1989-06-01 00:00:00

abstract：:MurG is a peripheral membrane protein that is one of the key enzymes in peptidoglycan biosynthesis. The crystal structure of Escherichia coli MurG (S. Ha, D. Walker, Y. Shi, and S. Walker, Protein Sci. 9:1045-1052, 2000) contains a hydrophobic patch surrounded by basic residues that may represent a membrane associatio...

journal_title：Journal of bacteriology

authors： van den Brink-van der Laan E,Boots JW,Spelbrink RE,Kool GM,Breukink E,Killian JA,de Kruijff B

更新日期：2003-07-01 00:00:00

abstract：:The UspA1 and UspA2 proteins of Moraxella catarrhalis are structurally related, are exposed on the bacterial cell surface, and migrate as very high-molecular-weight complexes in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Previous analysis of uspA1 and uspA2 mutants of M. catarrhalis strain 035E indicat...

journal_title：Journal of bacteriology

authors： Lafontaine ER,Cope LD,Aebi C,Latimer JL,McCracken GH Jr,Hansen EJ

更新日期：2000-03-01 00:00:00

abstract：:We sequenced the minimum REPI replication region and the incompatibility determinants of pColV-K30. The minimum replication region contains an open reading frame which corresponds to a 35-kilodalton (kDa) protein. Sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis with maxicells transformed with a numb...

journal_title：Journal of bacteriology

authors： Perez-Casal JF,Gammie AE,Crosa JH

更新日期：1989-04-01 00:00:00

abstract：:In Streptococcus mutans, enzyme II(scr) and sucrose-6-phosphate hydrolase are two important enzymes in the transport and metabolism of dietary sucrose. The scr regulon of S. mutans is composed of three genes, scrA and scrB, which code for enzyme II(scr) and sucrose-6-phosphate hydrolase, respectively, and scrR, which ...

journal_title：Journal of bacteriology

authors： Wang B,Kuramitsu HK

更新日期：2003-10-01 00:00:00

abstract：:Alkaline phosphatase (APase) is induced as a culture enters stationary phase because of limiting phosphate. The results presented here show that expression of APase is regulated both negatively and positively. PhoP, a homolog of a family of bacterial transcription factors, and PhoR, a homolog of bacterial histidine pr...

journal_title：Journal of bacteriology

authors： Jensen KK,Sharkova E,Duggan MF,Qi Y,Koide A,Hoch JA,Hulett FM

更新日期：1993-06-01 00:00:00

abstract：:The type II secretion system (main terminal branch of the general secretion pathway) is used by diverse gram-negative bacteria to secrete extracellular proteins. Proteins secreted by this pathway are synthesized with an N-terminal signal peptide which is removed upon translocation across the inner membrane, but the si...

journal_title：Journal of bacteriology

authors： Lindeberg M,Boyd CM,Keen NT,Collmer A

更新日期：1998-03-01 00:00:00

abstract：:Thermococcus kodakarensis (formerly Thermococcus kodakaraensis) strains have been constructed with synthetic and natural DNA sequences, predicted to function as archaeal transcription terminators, identically positioned between a constitutive promoter and a beta-glycosidase-encoding reporter gene (TK1761). Expression ...

journal_title：Journal of bacteriology

authors： Santangelo TJ,Cubonová L,Skinner KM,Reeve JN

更新日期：2009-11-01 00:00:00

abstract：:Random transposon Tn5 mutagenesis of Bradyrhizobium sp. (Arachis) strain NC92, a member of the cowpea cross-inoculation group, was carried out, and kanamycin-resistant transconjugants were tested for their symbiotic phenotype on three host plants: groundnut, siratro, and pigeonpea. Two nodulation (Nod- phenotype) muta...

journal_title：Journal of bacteriology

authors： Wilson KJ,Anjaiah V,Nambiar PT,Ausubel FM

更新日期：1987-05-01 00:00:00

abstract：:SugE of Escherichia coli, first identified as a suppressor of groEL mutations but a member of the small multidrug resistance family, has not previously been shown to confer a drug resistance phenotype. We show that high-level expression of sugE leads to resistance to a subset of toxic quaternary ammonium compounds. ...

journal_title：Journal of bacteriology

authors： Chung YJ,Saier MH Jr

更新日期：2002-05-01 00:00:00

abstract：:Overexpression of vsr in Escherichia coli stimulates transition and frameshift mutations. The pattern of mutations suggests that mutagenesis is due to saturation or inactivation of dam-directed mismatch repair. ...

journal_title：Journal of bacteriology

authors： Doiron KM,Viau S,Koutroumanis M,Cupples CG

更新日期：1996-07-01 00:00:00

abstract：:S1 nuclease mapping and Northern (RNA) hybridization revealed that the rpoDA gene encoding the principal sigma subunit of Pseudomonas aeruginosa PAO1 is transcribed as a monocistronic mRNA of 2 kb and that the transcription from the rpoDA promoter (PC) starts 32 bases upstream from the first nucleotide of the initiati...

journal_title：Journal of bacteriology

authors： Fujita M,Tanaka K,Takahashi H,Amemura A

更新日期：1993-02-01 00:00:00