Bacterial transposon Tn7 utilizes two different classes of target sites.

abstract：:An investigation of recombination events occurring in zygotes formed during conjugation has been carried out. The frequencies with which donor markers situated close to the origin are recovered indicates an obligatory interaction between the recipient chromosome and a region on the donor chromosome adjacent to the lea...

journal_title：Journal of bacteriology

authors： Pittard J,Walker EM

更新日期：1967-11-01 00:00:00

abstract：:Glycosylation is a common modulation of protein function in eukaryotes and is biologically important. However, in bacteria protein glycosylation is rare, and relatively few bacterial glycoproteins are known. In Escherichia coli only two glycoproteins have been described to date. Here we introduce a novel member to thi...

journal_title：Journal of bacteriology

authors： Sherlock O,Dobrindt U,Jensen JB,Munk Vejborg R,Klemm P

更新日期：2006-03-01 00:00:00

abstract：:The inducible galactose transport system in bakers' yeast carries out the facilitated diffusion of the nonmetabolized galactose analogues d-fucose and l-arabinose. This capacity depends on the activity of the Ga 2 gene. In some strains, d-fucose and l-arabinose are also gratuitous inducers. Mutants in which the induci...

journal_title：Journal of bacteriology

authors： Cirillo VP

更新日期：1968-05-01 00:00:00

abstract：:Complementation analyses using minimal recombinant clones showed that all known pdx point mutations, which cause pyridoxine (vitamin B6) or pyridoxal auxotrophy, are located in the pdxA, pdxB, serC, pdxJ, and pdxH genes. Antibiotic enrichments for chromosomal transposon mutants that require pyridoxine (vitamin B6) or ...

journal_title：Journal of bacteriology

authors： Lam HM,Tancula E,Dempsey WB,Winkler ME

更新日期：1992-03-01 00:00:00

abstract：:The activity of the ATP:GTP 3'-pyrophosphotransferase (guanosine pentaphosphate synthetase I [GPSI]) from Streptomyces antibioticus is stimulated maximally by methanol at 20% (vol/vol) in assay mixtures. Although the enzyme is not activated by ribosomes, its activity is stimulated by tRNA (uncharged or charged) and by...

journal_title：Journal of bacteriology

authors： Jones GH

更新日期：1994-03-01 00:00:00

abstract：:Bacteroides ruminicola B(1)4, a noncellulolytic rumen bacterium, produces an endoglucanase (carboxymethylcellulase [CMCase]) that is excreted into the culture supernatant. Cultures grown on glucose, fructose, maltose, mannose, and cellobiose had high specific activities of CMCase (greater than 3 mmol of reducing sugar...

journal_title：Journal of bacteriology

authors： Matsushita O,Russell JB,Wilson DB

更新日期：1990-07-01 00:00:00

abstract：:We characterized three distinct families of repeated sequences in the genome of the cyanobacterium Calothrix sp. strain PCC 7601. These repeated sequences were present at a level of about 100 copies per Calothrix genome and consisted of tandemly amplified heptanucleotides. These elements were named short tandemly repe...

journal_title：Journal of bacteriology

authors： Mazel D,Houmard J,Castets AM,Tandeau de Marsac N

更新日期：1990-05-01 00:00:00

abstract：:Attaching and effacing (AE) pathogens colonize the gut mucosa using a type three secretion system (T3SS) and a suite of effector proteins. The locus of enterocyte effacement (LEE) is the defining genetic feature of the AE pathogens, encoding the T3SS and the core effector proteins necessary for pathogenesis. Extensive...

journal_title：Journal of bacteriology

authors： Furniss RCD,Clements A

更新日期：2017-12-20 00:00:00

abstract：:A set of oligonucleotide primers capable of initiating enzymatic amplification (polymerase chain reaction) on a phylogenetically and taxonomically wide range of bacteria is described along with methods for their use and examples. One pair of primers is capable of amplifying nearly full-length 16S ribosomal DNA (rDNA) ...

journal_title：Journal of bacteriology

authors： Weisburg WG,Barns SM,Pelletier DA,Lane DJ

更新日期：1991-01-01 00:00:00

abstract：:Partial denaturation of Saccharomyces cerevisiae chromosomal DNA was found to occur spontaneously during meiosis. Short regions of strand separation (300 base pairs long) were seen in DNA molecules prepared for electron microscopy by the aqueous spreading technique. These regions were clustered along the DNA. The time...

journal_title：Journal of bacteriology

authors： Klein HL,Byers B

更新日期：1978-05-01 00:00:00

abstract：:Pili isolated from Pseudomonas aeruginosa PAK were solubilized in the detergent octyl-glucoside. Subsequent removal of the detergent by dialysis resulted in the formation of short rods of about 9 nm in diameter and various lengths. The aggregation process was followed by ultracentrifugation, viscometry, and electron m...

journal_title：Journal of bacteriology

authors： Watts TH,Scraba DG,Paranchych W

更新日期：1982-09-01 00:00:00

abstract：:A novel phenotype is described for Escherichia coli K-12 carrying the prlA4 allele determining a membrane component of the protein export mechanism. It is manifest as transformation deficiency for plasmids containing the cloned group C streptococcal streptokinase gene, skc. Streptokinase plasmid mutations relieving th...

journal_title：Journal of bacteriology

authors： Müller J,Reinert H,Malke H

更新日期：1989-04-01 00:00:00

abstract：:During sporulation in diploid Saccharomyces cerevisiae, spindle pole bodies acquire the so-called meiotic plaque, a prerequisite for spore formation. Mpc70p is a component of the meiotic plaque and is thus essential for spore formation. We show here that MPC70/mpc70 heterozygous strains most often produce two spores i...

journal_title：Journal of bacteriology

authors： Wesp A,Prinz S,Fink GR

更新日期：2001-04-01 00:00:00

abstract：:The electron transport system of Acinetobacter sp. HO1-N was studied to determine the specific cytochromes and to measure changes in the composition of the respiratory system due to growth in various concentrations of oxygen or types of growth substrates. Spectrophotometric analysis revealed that the quantity and type...

journal_title：Journal of bacteriology

authors： Ensley BD Jr,Finnerty WR

更新日期：1980-06-01 00:00:00

abstract：:The histidine kinase (HK) component of many two-component regulatory systems exhibits regulated ability to phosphorylate itself and to participate in transfer of phosphate to and from its cognate response regulator. The signaling system that controls expression of the UhpT sugar phosphate transporter in Escherichia co...

journal_title：Journal of bacteriology

authors： Wright JS,Olekhnovich IN,Touchie G,Kadner RJ

更新日期：2000-11-01 00:00:00

abstract：:Aerosols of Mycoplasma pneumoniae were studied at several relative humidities at a controlled temperature of 27 C. Production of an experimentally reproducible aerosol required preatomization of the organism in its suspending fluid and was dependent on the type of fluid used in atomization as well as on the procedures...

journal_title：Journal of bacteriology

authors： Wright DN,Bailey GD,Hatch MT

更新日期：1968-10-01 00:00:00

abstract：:Type III secretion systems (T3SSs) are essential virulence devices for many gram-negative bacteria that are pathogenic for plants, animals, and humans. They serve to translocate virulence effector proteins directly into eukaryotic host cells. T3SSs are composed of a large cytoplasmic bulb and a transmembrane region in...

journal_title：Journal of bacteriology

authors： Veenendaal AK,Sundin C,Blocker AJ

更新日期：2009-01-01 00:00:00

abstract：:The secondary metabolite hydrogen cyanide (HCN) is produced by Pseudomonas fluorescens from glycine, essentially under microaerophilic conditions. The genetic basis of HCN synthesis in P. fluorescens CHA0 was investigated. The contiguous structural genes hcnABC encoding HCN synthase were expressed from the T7 promoter...

journal_title：Journal of bacteriology

authors： Laville J,Blumer C,Von Schroetter C,Gaia V,Défago G,Keel C,Haas D

更新日期：1998-06-01 00:00:00

abstract：:During growth of streptomycin-dependent strains of Escherichia coli in the absence of streptomycin (deprived growth), both constitutive and inducible synthesis of beta-galactosidase were preferentially inhibited. A similar preferential inhibition of constitutive and derepressed synthesis of alkaline phosphatase was ob...

journal_title：Journal of bacteriology

authors： Goodman RE,Spotts CR

更新日期：1967-10-01 00:00:00

abstract：:The DNA binding protein WhiA is conserved in Gram-positive bacteria and is present in the genetically simple cell wall-lacking mycoplasmas. The protein shows homology to eukaryotic homing endonucleases but lacks nuclease activity. WhiA was first characterized in streptomycetes, where it regulates the expression of key...

journal_title：Journal of bacteriology

authors： Bohorquez LC,Surdova K,Jonker MJ,Hamoen LW

更新日期：2018-03-26 00:00:00

abstract：:The ompR and envZ genes, which together constitute the ompB operon, are involved in osmoregulatory expression of the OmpF and OmpC proteins, major outer membrane proteins of Escherichia coli. The envZ11 mutation results in the OmpF- OmpC-constitutive phenotype. A mutant which suppressed defects caused by the envZ11 mu...

journal_title：Journal of bacteriology

authors： Matsuyama S,Mizuno T,Mizushima S

更新日期：1986-12-01 00:00:00

abstract：:Broad-host-range plasmid RK2 encodes several different kil genes which are potentially lethal to an Escherichia coli host. The kil genes and the essential RK2 replication gene trfA are regulated by the products of kor genes. We have shown previously that kilA can be controlled by a constitutively expressed korA gene. ...

journal_title：Journal of bacteriology

authors： Young C,Burlage RS,Figurski DH

更新日期：1987-03-01 00:00:00

abstract：:Hauge, Jens G. (National Institute for Public Health, Oslo, Norway). Glucose dehydrogenation in bacteria: a comparative study. J. Bacteriol. 82:609-614. 1961.-Extracts of a series of bacteria reported to convert glucose to gluconic acid via particulate enzyme systems were fractionated by differential centrifugation in...

journal_title：Journal of bacteriology

authors： HAUGE JG

更新日期：1961-10-01 00:00:00

abstract：:Gene sequences encoding the enzymes UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) from many bacterial sources were analyzed. It was shown that whereas gram-negative bacteria have only one murA gene, gram-positive bacteria have two distinct genes encoding these enzymes which have possibly arisen from gene dupl...

journal_title：Journal of bacteriology

authors： Du W,Brown JR,Sylvester DR,Huang J,Chalker AF,So CY,Holmes DJ,Payne DJ,Wallis NG

更新日期：2000-08-01 00:00:00

abstract：:The whole-genome sequence of Mycobacterium bolletii M24, isolated from the bronchoalveolar lavage fluid of a Malaysian patient, is reported here. The circular chromosome of 5,507,730 bp helped to clarify the taxonomic position of this organism within the M. abscessus complex and revealed the presence of proteins poten...

journal_title：Journal of bacteriology

authors： Wong YL,Choo SW,Tan JL,Ong CS,Ng KP,Ngeow YF

更新日期：2012-08-01 00:00:00

abstract：:Porin F is one of the major proteins of the outer membrane of Pseudomonas aeruginosa. It forms water-filled pores of variable size. Porin F is a candidate for a vaccine against P. aeruginosa because it antigenically cross-reacts in all serotype strains of the International Antigenic Typing Scheme. We have isolated the...

journal_title：Journal of bacteriology

authors： Duchêne M,Schweizer A,Lottspeich F,Krauss G,Marget M,Vogel K,von Specht BU,Domdey H

更新日期：1988-01-01 00:00:00

abstract：:A new osmotically inducible gene in Escherichia coli, osmY, was induced 8- to 10-fold by hyperosmotic stress and 2- to 3-fold by growth in complex medium. The osmY gene product is a periplasmic protein which migrates with an apparent molecular mass of 22 kDa on sodium dodecyl sulfate-polyacrylamide gels. A genetic fus...

journal_title：Journal of bacteriology

authors： Yim HH,Villarejo M

更新日期：1992-06-01 00:00:00

abstract：:Comprehensive analysis of genome-wide expression patterns during growth of the hyperthermophilic bacterium Thermotoga maritima on 14 monosaccharide and polysaccharide substrates was undertaken with the goal of proposing carbohydrate specificities for transport systems and putative transcriptional regulators. Saccharid...

journal_title：Journal of bacteriology

authors： Conners SB,Montero CI,Comfort DA,Shockley KR,Johnson MR,Chhabra SR,Kelly RM

更新日期：2005-11-01 00:00:00

abstract：:A sucrose-inducible alpha-glucosidase activity that hydrolyzes sucrose in Candida albicans has been demonstrated previously. The enzyme is assayable in whole cells and was inhibited by both sucrose and maltose. A C. albicans gene (CASUC1) that affects sucrose utilization and alpha-glucosidase activity was cloned by ex...

journal_title：Journal of bacteriology

authors： Kelly R,Kwon-Chung KJ

更新日期：1992-01-01 00:00:00

abstract：:An alcohol dehydrogenase (ADH) from hyperthermophilic archaeon Thermococcus guaymasensis was purified to homogeneity and was found to be a homotetramer with a subunit size of 40 ± 1 kDa. The gene encoding the enzyme was cloned and sequenced; this gene had 1,095 bp, corresponding to 365 amino acids, and showed high seq...

journal_title：Journal of bacteriology

authors： Ying X,Ma K

更新日期：2011-06-01 00:00:00