osmY, a new hyperosmotically inducible gene, encodes a periplasmic protein in Escherichia coli.

Abstract:

:A new osmotically inducible gene in Escherichia coli, osmY, was induced 8- to 10-fold by hyperosmotic stress and 2- to 3-fold by growth in complex medium. The osmY gene product is a periplasmic protein which migrates with an apparent molecular mass of 22 kDa on sodium dodecyl sulfate-polyacrylamide gels. A genetic fusion to osmY was mapped to 99.3 min on the E. coli chromosome. The gene was cloned and sequenced, and an open reading frame was identified. The open reading frame encoded a precursor protein with a calculated molecular weight of 21,090 and a mature protein of 18,150 following signal peptide cleavage. Sequencing of the periplasmic OsmY protein confirmed the open reading frame and defined the signal peptide cleavage site as Ala-Glu. A mutation caused by the osmY::TnphoA genetic fusion resulted in slightly increased sensitivity to hyperosmotic stress.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Yim HH,Villarejo M

doi

10.1128/jb.174.11.3637-3644.1992

keywords:

subject

Has Abstract

pub_date

1992-06-01 00:00:00

pages

3637-44

issue

11

eissn

0021-9193

issn

1098-5530

journal_volume

174

pub_type

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