Formation of 9-nm filaments from pilin monomers obtained by octyl-glucoside dissociation of Pseudomonas aeruginosa pili.

Abstract:

:Pili isolated from Pseudomonas aeruginosa PAK were solubilized in the detergent octyl-glucoside. Subsequent removal of the detergent by dialysis resulted in the formation of short rods of about 9 nm in diameter and various lengths. The aggregation process was followed by ultracentrifugation, viscometry, and electron microscopy to show that the aggregates produced in this way are distinct from pili produced by the bacteria, both in diameter, as measured by electron micrographs, and in their inability to compete in a biological assay for pili.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Watts TH,Scraba DG,Paranchych W

doi

10.1128/JB.151.3.1508-1513.1982

subject

Has Abstract

pub_date

1982-09-01 00:00:00

pages

1508-13

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

151

pub_type

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