Secretion of LamB-LacZ by the signal recognition particle pathway of Escherichia coli.

abstract：:In Escherichia coli, ClpYQ (HslUV) is a two-component ATP-dependent protease composed of ClpY (HslU), an ATPase with unfolding activity, and ClpQ (HslV), a peptidase. In the ClpYQ proteolytic complex, the hexameric rings of ClpY (HslU) are responsible for protein recognition, unfolding, and translocation into the prot...

journal_title：Journal of bacteriology

authors： Hsieh FC,Chen CT,Weng YT,Peng SS,Chen YC,Huang LY,Hu HT,Wu YL,Lin NC,Wu WF

更新日期：2011-10-01 00:00:00

abstract：:Lactobacillus helveticus R0052 is a commercially available strain that is widely used in probiotic preparations. The genome sequence consisted of 2,129,425 bases. Comparative analysis showed that it was unique among L. helveticus strains in that it contained genes encoding mucus-binding proteins similar to those found...

journal_title：Journal of bacteriology

authors： Tompkins TA,Barreau G,Broadbent JR

更新日期：2012-11-01 00:00:00

abstract：:The human bacterial pathogens Chlamydia spp. possess a genus-specific lipopolysaccharide as a major surface antigen, the structure of which has been determined by analytical chemistry as Kdop alpha 2-8-Kdop alpha 2-4-Kdop alpha 2-6GlcNp beta 1-6-GlcNol (Kdo, 3-deoxy-D-manno-2-octulosonic acid). Immunochemical studies ...

journal_title：Journal of bacteriology

authors： Holst O,Brade L,Kosma P,Brade H

更新日期：1991-03-01 00:00:00

abstract：:We previously elucidated the global transcriptional responses of Escherichia coli to the nitrosating agent S-nitrosoglutathione (GSNO) in both aerobic and anaerobic chemostats, demonstrated the expression of nitric oxide (NO)-protective mechanisms, and obtained evidence of critical thiol nitrosation. The present study...

journal_title：Journal of bacteriology

authors： Pullan ST,Gidley MD,Jones RA,Barrett J,Stevanin TM,Read RC,Green J,Poole RK

更新日期：2007-03-01 00:00:00

abstract：:Double mutants which carry mutations in genes (rodA, pbpA) required for cell elongation (i.e., maintenance of rod shape) in combination with mutations in genes (ftsA, ftsI, ftsQ, or ftsZ) required for septation were constructed. Such mutants were able to grow for about two mass doublings at a normal rate at the restri...

journal_title：Journal of bacteriology

authors： Begg KJ,Donachie WD

更新日期：1985-08-01 00:00:00

abstract：:Tryptophan auxotrophs of the archaebacterium Haloferax volcanii define a cluster of overlapping genes homologous to eubacterial-eukaryotic trpD, -F, -E, and -G, linked in that order and each preceded by a possible ribosome binding site. Residues involved in feedback inhibition of eubacterial anthranilate synthetases a...

journal_title：Journal of bacteriology

authors： Lam WL,Logan SM,Doolittle WF

更新日期：1992-03-01 00:00:00

abstract：:When Bacillus megaterium spores germinate in the absence of an exogenous carbon source, the first minutes of germination are accompanied by production of large amounts (approximately 70 nmol/mg of dry spores) of acetate and much smaller amounts of pyruvate and lactate. The majority of these compounds are excreted into...

journal_title：Journal of bacteriology

authors： Setlow B,Shay LK,Vary JC,Setlow P

更新日期：1977-11-01 00:00:00

abstract：:Twenty-four independent phosphate starvation-inducible (psi) transcriptional fusions made with Mu d1(lacZbla) were analyzed by sequencing the psi::lacZ(Mu d1) chromosomal junctions by using DNAs amplified with the polymerase chain reaction or mini-Mu cloning. Our DNA sequence analysis showed that the MuR DNA in Mu d1 ...

journal_title：Journal of bacteriology

authors： Metcalf WW,Steed PM,Wanner BL

更新日期：1990-06-01 00:00:00

abstract：:Evidence is presented that progametangia in both the plus and the minus mating types of Mucor mucedo can be induced by one substance, namely (-)-trisporic acid B. A method is described for the determination of the concentration of the sex factors (trisporone, trisporic acid B, trisporic acid C) in mated cultures of Mu...

journal_title：Journal of bacteriology

authors： Van den Ende H,Wiechmann AH,Reyngoud DJ,Hendriks T

更新日期：1970-02-01 00:00:00

abstract：:Halobacterium halobium spontaneously reverses its swimming direction with a frequency of about 0.1 s-1. The self-sustained periodicity can be entrained by rhythmic light stimuli within a limited range between 0.13 and 0.3 s-1. Increase of temperature increases the reversal frequency and shortens the time to reach maxi...

journal_title：Journal of bacteriology

authors： Schimz A,Hildebrand E

更新日期：1986-05-01 00:00:00

abstract：:The effects of high doses of radiation (1, 5, or 20 Mrad) on the toxicity, pyrogenicity, and immunogenicity of Salmonella typhimurium cells and endotoxin were studied. Toxicity decreased progressively after exposure to 1, 5, or 20 Mrad. The lethal effect of 1-Mrad exposed cells was greater than that of heat-, acetone-...

journal_title：Journal of bacteriology

authors： Previte JJ

更新日期：1968-06-01 00:00:00

abstract：:Thermococcus sp. strain 4557 is a hyperthermophilic anaerobic archaeon isolated from the deep-sea hydrothermal vent Guaymas Basin site in the Gulf of California at a depth of 2,000 m. Here, we present the complete genome sequence of Thermococcus sp. 4557, which consists of a single circular chromosome of 2,011,320 bp ...

journal_title：Journal of bacteriology

authors： Wang X,Gao Z,Xu X,Ruan L

更新日期：2011-10-01 00:00:00

abstract：:Ramakrishnan, T. (Yale University, New Haven, Conn.), and Edward A. Adelberg. Regulatory mechanisms in the biosynthesis of isoleucine and valine. II. Identification of two operator genes. J. Bacteriol. 89:654-660. 1965.-A tightly clustered set of five structural genes governs the synthesis of the five enzymes of isole...

journal_title：Journal of bacteriology

authors： RAMAKRISHNAN T,ADELBERG EA

更新日期：1965-03-01 00:00:00

abstract：:Tn916 [carries tet(M)] is a 16.4-kb conjugative transposon that can establish itself in multiple copies in Enterococcus faecalis. To study the interaction of coresident homologous transposons during conjugation, an E. faecalis mutant defective in homologous recombination was utilized for construction of strains harbor...

journal_title：Journal of bacteriology

authors： Flannagan SE,Clewell DB

更新日期：1991-11-01 00:00:00

abstract：:Host range mutants of phage T1 (T1h), which productively infected tonB mutants of Escherichia coli, were isolated. The phage mutants were inactivated by isolated outer membranes of E. coli in contrast to the wild-type phage, which only adsorbed reversibly. For the infection process, the tonB function is apparently onl...

journal_title：Journal of bacteriology

authors： Hantke K,Braun V

更新日期：1978-07-01 00:00:00

abstract：:The celA1 gene encoding an endo-beta-1,4-glucanase from a mesophilic actinomycete, strain JM8, identified as Streptomyces halstedii, was cloned and expressed in S. lividans JI66. From the nucleotide sequence of a 1.7-kb DNA fragment we identified an open reading frame of 963 nucleotides encoding a protein of 321 amino...

journal_title：Journal of bacteriology

authors： Fernández-Abalos JM,Sánchez P,Coll PM,Villanueva JR,Pérez P,Santamaría RI

更新日期：1992-10-01 00:00:00

abstract：:The activity of the ATP:GTP 3'-pyrophosphotransferase (guanosine pentaphosphate synthetase I [GPSI]) from Streptomyces antibioticus is stimulated maximally by methanol at 20% (vol/vol) in assay mixtures. Although the enzyme is not activated by ribosomes, its activity is stimulated by tRNA (uncharged or charged) and by...

journal_title：Journal of bacteriology

authors： Jones GH

更新日期：1994-03-01 00:00:00

abstract：:Flac mutants insensitive to transfer inhibition by R factors. JR66a and R485 were isolated and characterized. Representative mutations were cis dominant and are therefore presumed to be at the sites of action, fisU and fisV, respectively, of the FinU and FinV transfer inhibition systems encoded by JR66a and R485. The ...

journal_title：Journal of bacteriology

authors： Gasson MJ,Willetts NS

更新日期：1977-08-01 00:00:00

abstract：:The distribution of glycerophospholipid-cholesterol acyltransferase in selected bacterial species was examined. Enzyme activity was demonstrated in cell-free growth media from all members of the family Vibrionaceae which were tested except Plesiomonas shigelloides. In each case, enzyme was produced in exponential to e...

journal_title：Journal of bacteriology

authors： MacIntyre S,Trust TJ,Buckley JT

更新日期：1979-07-01 00:00:00

abstract：:A general strategy for cloning the functional homologs of an Escherichia coli gene was used to clone homologs of 4.5S RNA from other bacteria. The genes encoding these homologs were selected by their ability to complement a deletion of the gene for 4.5S RNA. DNA sequences of the regions encoding the homologs were dete...

journal_title：Journal of bacteriology

authors： Brown S,Thon G,Tolentino E

更新日期：1989-12-01 00:00:00

abstract：:The BacA protein is essential for the long-term survival of Sinorhizobium meliloti and Brucella abortus within acidic compartments in plant and animal cells, respectively. Since both the S. meliloti and B. abortus bacA mutants have an increased resistance to bleomycin, it was hypothesized that BacA was a transporter o...

journal_title：Journal of bacteriology

authors： Ferguson GP,Roop RM 2nd,Walker GC

更新日期：2002-10-01 00:00:00

abstract：:An R plasmid, R100-1, was mapped previously (Yoshikawa, 1974) by transduction from an integratively suppressed Hfr strain to a recipient with a mutation in gene dnaA. By this method various types of transductants of plasmid R100-1 that exist autonomously or in the integrated state were obtained. Seventy-one such trans...

journal_title：Journal of bacteriology

authors： Yoshikawa M

更新日期：1975-12-01 00:00:00

abstract：:Regulation of the cadA cadmium and zinc resistance determinant of Staphylococcus aureus plasmid pI258 was demonstrated by using gene fusions and direct measurements of transcription. In growth experiments, cells harboring the intact cadA operon were induced with different cations and challenged by an inhibitory concen...

journal_title：Journal of bacteriology

authors： Yoon KP,Misra TK,Silver S

更新日期：1991-12-01 00:00:00

abstract：:In the nitrogenase system of Klebsiella pneumoniae, nifT is located between nifDK, the structural genes for dinitrogenase, and nifY, whose product is involved in nitrogenase maturation. It is, therefore, a reasonable hypothesis that the NifT protein might also have a role in the maturation of nitrogenase. However, the...

journal_title：Journal of bacteriology

authors： Simon HM,Homer MJ,Roberts GP

更新日期：1996-05-01 00:00:00

abstract：:Degenerate oligonucleotides based on the published Escherichia coli glutamate decarboxylase (GAD) protein sequence were used in a polymerase chain reaction to generate a DNA probe for the E. coli GAD structural gene. Southern blots showed that there were two cross-hybridizing GAD genes, and both of these were cloned a...

journal_title：Journal of bacteriology

authors： Smith DK,Kassam T,Singh B,Elliott JF

更新日期：1992-09-01 00:00:00

abstract：:Protocatechuate 3,4-dioxygenase is a member of a family of bacterial enzymes that cleave the aromatic rings of their substrates between two adjacent hydroxyl groups, a key reaction in microbial metabolism of varied environmental chemicals. In an appropriate genetic background, it is possible to select for Acinetobacte...

journal_title：Journal of bacteriology

authors： D'Argenio DA,Vetting MW,Ohlendorf DH,Ornston LN

更新日期：1999-10-01 00:00:00

abstract：:Guanine is transported into germinated conidia of Neurospora crassa by the general purine base transport system. Guanine uptake is inhibited by adenine and hypoxanthine but not xanthine. Guanine phosphoribosyltransferase (GPRTase) activity was demonstrated in cell extracts of wild-type germinated conidia. The Km for g...

journal_title：Journal of bacteriology

authors： Magill CW,Sabina RL,Garber TL,Magill JM

更新日期：1982-03-01 00:00:00

abstract：:Partial purification of a cytochrome bd complex from Azotobacter vinelandii grown under high aeration was achieved by isolating respiratory particles enriched in this hemoprotein via differential centrifugation and detergent extraction. The cytochrome bd complex was subsequently solubilized from the inner membrane wit...

journal_title：Journal of bacteriology

authors： Kolonay JF Jr,Moshiri F,Gennis RB,Kaysser TM,Maier RJ

更新日期：1994-07-01 00:00:00

abstract：:On the basis of information available in the literature, gluconate dissimilation in Escherichia coli is thought to occur via the hexose monophosphate pathway. Evidence is presented in this study that gluconate is catabolized in this organism via an inducible Entner-Doudoroff pathway. This evidence is based on chromato...

journal_title：Journal of bacteriology

authors： Eisenberg RC,Dobrogosz WJ

更新日期：1967-03-01 00:00:00

abstract：:The nickel-cobalt-cadmium resistance genes carried by plasmid pTOM9 of Alcaligenes xylosoxidans 31A are located on a 14.5-kb BamHI fragment. By random Tn5 insertion mutagenesis, the fragment was shown to contain two distinct nickel resistance loci, ncc and nre. The ncc locus causes a high-level combined nickel, cobalt...

journal_title：Journal of bacteriology

authors： Schmidt T,Schlegel HG

更新日期：1994-11-01 00:00:00