Abstract:
:The effects of high doses of radiation (1, 5, or 20 Mrad) on the toxicity, pyrogenicity, and immunogenicity of Salmonella typhimurium cells and endotoxin were studied. Toxicity decreased progressively after exposure to 1, 5, or 20 Mrad. The lethal effect of 1-Mrad exposed cells was greater than that of heat-, acetone-, or alcohol-killed preparations. An amount of 5 Mrad is about a 50% end point in terms of inactivation of the lethal lipopolysaccharide or cell-associated determinants. The fever response to radiation-killed salmonellae decreased between 1- and 20-Mrad exposure. The immunogenicity of 1-Mrad-treated cells usually exceeded that of nonirradiated preparations in mouse-protection tests. With increasing radiation doses, there was a dramatic decrease in, but not an abolition of, immunogenicity. Preparations exposed to 20 Mrad which were nonlethal afforded significant protection. The results are interpreted as a reflection of a dissociation of the primary and secondary toxic determinants of endotoxin after irradiation. The data indicate the potential value of radiation sterilization as a means of production of Salmonella vaccine.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Previte JJdoi
10.1128/JB.95.6.2165-2170.1968subject
Has Abstractpub_date
1968-06-01 00:00:00pages
2165-70issue
6eissn
0021-9193issn
1098-5530journal_volume
95pub_type
杂志文章abstract::Deletions in the tet genes derived from Tn10 were formed from different tet::Tn5 insertion mutations by removing DNA sequences located between a HindIII site in Tn5 and a HindIII site adjacent to the tet genes. Tetracycline-sensitive point mutations were mapped in recombination tests with the deletions and were thus a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.2.921-929.1983
更新日期:1983-02-01 00:00:00
abstract::Eight mutants of Bacillus subtilis deficient in xylan utilization were isolated and characterized genetically and biochemically. Each mutant was obtained independently after nitrosoguanidine mutagenesis. All of the analyzed mutations were shown to be linked. Reciprocal transformation crosses revealed the existence of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.1.257-263.1983
更新日期:1983-10-01 00:00:00
abstract::Vacuoles project streams of vesicles and membranous tubules into the yeast bud where they fuse, founding the daughter cell organelle, vac5-1, which encodes a truncated form of the Pho80 cyclin, inhibits normal vacuole inheritance. An in vitro inheritance assay which measures the fusion of vacuoles serves as a model fo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.14.4047-4051.1996
更新日期:1996-07-01 00:00:00
abstract::Streptococcus suis serotype 2 (S. suis 2) is an important swine and human pathogen responsible for septicemia and meningitis. A novel gene, designated atl and encoding a major autolysin of S. suis 2 virulent strain HA9801, was identified and characterized in this study. The Atl protein contains 1,025 amino acids with ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06231-11
更新日期:2012-03-01 00:00:00
abstract::An abundant 19.3-kDa Helicobacter pylori protein has been cloned, and the sequence is homologous with a ferritin-like protein produced by Escherichia coli K-12. Homologies are also present with a number of eucaryotic ferritins, as well as with the heme group-containing bacterioferritins. All amino acids involved in ch...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.4.966-972.1993
更新日期:1993-02-01 00:00:00
abstract::Median longitudinal sections of germ tube apices of Mucor rouxii revealed the presence of a single, roughly hemispherical, electron-dense organelle, in intimate contact with the apical cell wall. Conceivably, this "apical corpuscle" may be responsible for the emission of the germ tube and its continued apical growth. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.6.2399-2402.1968
更新日期:1968-06-01 00:00:00
abstract::Plasmid pP51 of Pseudomonas sp. strain P51 contains two gene clusters encoding the degradation of chlorinated benzenes, tcbAB and tcbCDEF. A regulatory gene, tcbR, was located upstream and divergently transcribed from the chlorocatechol oxidative gene cluster tcbCDEF. The tcbR gene was characterized by DNA sequencing ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.12.3700-3708.1991
更新日期:1991-06-01 00:00:00
abstract::The phoP-phoQ-regulated pagC locus is essential for full virulence and survival within macrophages of Salmonella typhimurium. The protein product, DNA sequence, and transcript of pagC were determined. The pagC locus encodes a single 188-amino-acid membrane protein that is similar to the ail-encoded eucaryotic cell inv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.1.86-93.1991
更新日期:1991-01-01 00:00:00
abstract::Resistance of Staphylococcus aureus mediated by the penicillinase (Pc-ase) plasmid to divalent metal ions of Hg and Cd was found to be controlled by different mechanisms. The Hg resistance of the Pc-ase plasmid-carrying organisms is based upon a process of changing the ion incorporated in the cell into a somewhat inno...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.117.1.1-7.1974
更新日期:1974-01-01 00:00:00
abstract::The mutualistic symbiont Vibrio fischeri builds a symbiotic biofilm during colonization of squid hosts. Regulation of the exopolysaccharide component, termed Syp, has been examined in strain ES114, where production is controlled by a phosphorelay that includes the inner membrane hybrid histidine kinase RscS. Most stra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00033-19
更新日期:2019-04-09 00:00:00
abstract::A draft genome sequence of Tsukamurella sp., an aerobic bacterium isolated from a human sputum specimen, is described here. A new virus or provirus, TPA4, was characterized. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01279-12
更新日期:2012-10-01 00:00:00
abstract::A method was devised for the isolation of yeast monomorphic (LEV) mutants of Candida albicans. By this procedure, about 20 stable yeast-like mutants were isolated after mutagenesis with ethyl methane sulfonate. The growth rate of the mutants in different carbon sources, both fermentable and not, was indistinguishable ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.8.2318-2325.1994
更新日期:1994-04-01 00:00:00
abstract::Sulfur-starved cells of Anacystis nidulans have an increased capacity to take up sulfate. The apparent Vmax for sulfate uptake increased at least 10-fold after 24 h of sulfur deprivation, whereas the K1/2 remained unchanged at approximately 1.35 microM. The initial rate of sulfate uptake increased between 2 and 6 h af...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.2.583-587.1988
更新日期:1988-02-01 00:00:00
abstract::The nickel-cobalt-cadmium resistance genes carried by plasmid pTOM9 of Alcaligenes xylosoxidans 31A are located on a 14.5-kb BamHI fragment. By random Tn5 insertion mutagenesis, the fragment was shown to contain two distinct nickel resistance loci, ncc and nre. The ncc locus causes a high-level combined nickel, cobalt...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.22.7045-7054.1994
更新日期:1994-11-01 00:00:00
abstract::Protein and mRNA levels of heat-labile enterotoxin (LT) of Escherichia coli are highest at 37 degrees C, and they decrease gradually as temperature is decreased. This temperature effect is eliminated in an Hns- mutant. Deletion of portions of DNA coding for the LT A subunit also results in an increase in LT expression...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.14.3715-3718.1998
更新日期:1998-07-01 00:00:00
abstract::Leucine and isoleucine-valine loci have been mapped in Rhizobium japonicum. Transformation analysis suggests a common pathway for isoleucine-valine biosynthesis. Three-point reciprocal crosses indicated that all the leucine loci are not genetically linked. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.126.2.997-998.1976
更新日期:1976-05-01 00:00:00
abstract::It previously has been shown that lkyD mutants of Salmonella typhimurium form large blebs of outer membrane over the septal and polar regions of dividing cells. To determine whether the outer membrane blebs are formed over potential sites of division even in the absence of septal ingrowth, lkyD strains were studied un...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.2.1019-1024.1980
更新日期:1980-08-01 00:00:00
abstract::The pckA gene of Rhizobium meliloti, encoding phosphoenolpyruvate carboxykinase, was isolated from a genomic cosmid library by complementation of the succinate growth phenotype of a Pck- mutant. The gene region was mapped by subcloning and Tn5 insertion mutagenesis. The DNA sequence for a 2-kb region containing the st...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.6.1452-1460.1995
更新日期:1995-03-01 00:00:00
abstract::The enzyme diversity of the cellulolytic system produced by Clostridium cellulolyticum grown on crystalline cellulose as a sole carbon and energy source was explored by two-dimensional electrophoresis. The cellulolytic system of C. cellulolyticum is composed of at least 30 dockerin-containing proteins (designated cell...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00917-06
更新日期:2007-03-01 00:00:00
abstract::Maleylacetate reductase (EC 1.3.1.32) plays a major role in the degradation of chloroaromatic compounds by channelling maleylacetate and some chlorinated derivatives into the 3-oxoadipate pathway. Several substituted maleylacetates were prepared in situ by alkaline or enzymatic hydrolysis of dienelactones as the precu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.2.320-325.1995
更新日期:1995-01-01 00:00:00
abstract::Treatment of pneumococci with activator (a protein that induces bacterial "competence" to absorb deoxyribonucleic acid molecules and undergo genetic transformation) can cause either protoplast formation or leakage of intracellular components to the medium depending on postincubation conditions. The leaked intracellula...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.1.344-353.1975
更新日期:1975-01-01 00:00:00
abstract::Pantothenate kinase (CoaA) is a key regulator of coenzyme A (CoA) biosynthesis in Escherichia coli, and its activity is controlled by feedback inhibition by CoA and its thioesters. The importance of feedback inhibition in the control of the intracellular CoA levels was tested by constructing three site-directed mutant...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.11.3410-3415.2003
更新日期:2003-06-01 00:00:00
abstract::Expression of the lysine decarboxylase gene (cadA) of Escherichia coli is induced upon external acidification. To dissect the molecular mechanisms responsible for this regulation, we analyzed a 4.2-kbp region upstream from cadA. DNA sequencing revealed two long open reading frames upstream of and on the same strand as...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.2.530-540.1992
更新日期:1992-01-01 00:00:00
abstract::A new bacteriocin, termed lactococcin A (LCN-A), from Lactococcus lactis subsp. cremoris LMG 2130 was purified and sequenced. The polypeptide contained no unusual amino acids and showed no significant sequence similarity to other known proteins. Only lactococci were killed by the bacteriocin. Of more than 120 L. lacti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.12.3879-3887.1991
更新日期:1991-06-01 00:00:00
abstract::Rickettsia rickettsii, the causative agent of Rocky Mountain spotted fever, contains two immunodominant proteins, rOmpA and rOmpB, in the outer membrane. Both rOmpA and rOmpB are conserved throughout spotted fever group rickettsiae as members of a family of autotransporter proteins. Previously, it was demonstrated tha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00826-16
更新日期:2017-02-28 00:00:00
abstract::Expression of the Escherichia coli proU operon, which encodes an efficient uptake system for the osmoprotectant glycine betaine, is strongly increased in cells grown at high osmolarity. We isolated 182 independent spontaneous mutants with elevated expression of the chromosomal phi(proV-lacZ) (Hyb2) fusion at low osmol...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.2.801-809.1991
更新日期:1991-01-01 00:00:00
abstract::Partial purification of a cytochrome bd complex from Azotobacter vinelandii grown under high aeration was achieved by isolating respiratory particles enriched in this hemoprotein via differential centrifugation and detergent extraction. The cytochrome bd complex was subsequently solubilized from the inner membrane wit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.13.4177-4181.1994
更新日期:1994-07-01 00:00:00
abstract::Monobromoacetate (MBA) is toxic for the 1,2-dichloroethane-degrading bacterium Xanthobacter autotrophicus GJ10 at concentrations higher than 5 mM. Mutants which are able to grow on higher concentrations of MBA were isolated and found to overexpress haloacid dehalogenase, which is encoded by the dhlB gene. In mutant GJ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.5.1348-1356.1995
更新日期:1995-03-01 00:00:00
abstract:UNLABELLED:An ability to sense and respond to changes in extracellular phosphate is critical for the survival of most bacteria. For Caulobacter crescentus, which typically lives in phosphate-limited environments, this process is especially crucial. Like many bacteria, Caulobacter responds to phosphate limitation throug...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00658-15
更新日期:2015-10-19 00:00:00
abstract::Bishop, D. G. (University of Sydney, Sydney, Australia), and J. L. Still. Fatty acid metabolism in Serratia marcescens. J. Bacteriol. 82:370-375. 1961.-A study has been made of the oxidation of saturated fatty acids containing between two and 18 carbon atoms by whole cells of the bacterium, Serratia marcescens. This o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.3.370-375.1961
更新日期:1961-09-01 00:00:00