Abstract:
:An abundant 19.3-kDa Helicobacter pylori protein has been cloned, and the sequence is homologous with a ferritin-like protein produced by Escherichia coli K-12. Homologies are also present with a number of eucaryotic ferritins, as well as with the heme group-containing bacterioferritins. All amino acids involved in chelation of inorganic iron by ferritins from humans and other higher species are conserved in the H. pylori protein. Consistent with the structural data indicating an iron-binding function, E. coli overexpressing the H. pylori ferritin-like protein accumulates almost 10 times more nonheme iron than vector controls, and the iron-binding activity copurifies with the 19.3-kDa protein. Immunoelectron microscopy of H. pylori, as well as of E. coli overexpressing the H. pylori gene, demonstrates that the gene product has a cytoplasmic location where it forms paracrystalline inclusions. On the basis of these structural and functional data, we propose that the H. pylori gene product (termed Pfr) forms the basis for a second class of bacterial ferritins designed to store nonheme iron.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Frazier BA,Pfeifer JD,Russell DG,Falk P,Olsén AN,Hammar M,Westblom TU,Normark SJdoi
10.1128/jb.175.4.966-972.1993subject
Has Abstractpub_date
1993-02-01 00:00:00pages
966-72issue
4eissn
0021-9193issn
1098-5530journal_volume
175pub_type
杂志文章abstract:UNLABELLED:Mycobacterium tuberculosis possesses a thick and highly hydrophobic cell wall principally composed of a mycolyl-arabinogalactan-peptidoglycan complex, which is critical for survival and virulence. DprE1 is a well-characterized component of decaprenyl-phospho-ribose epimerase, which produces decaprenyl-phosph...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00628-15
更新日期:2015-12-01 00:00:00
abstract::Neisseria gonorrhoeae (gonococci) and Neisseria meningitidis (meningococci) are human pathogens that cause gonorrhea and meningococcal meningitis, respectively. Both N. gonorrhoeae and N. meningitidis release a number of small peptidoglycan (PG) fragments, including proinflammatory PG monomers, although N. meningitidi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00437-16
更新日期:2016-10-07 00:00:00
abstract::The rnt gene encoding RNase T was cloned on a 13-kilobase BamHI fragment. Restriction analysis of the fragment and comparison of it with the Escherichia coli restriction map localized rnt to kilobase coordinates 1733 to 1746, corresponding to about 36 min on the genetic map. The map location was confirmed by cotransdu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.10.5736-5737.1989
更新日期:1989-10-01 00:00:00
abstract:UNLABELLED:TcpP and ToxR coordinately regulate transcription of toxT, the master regulator of numerous virulence factors in Vibrio cholerae. TcpP and ToxR are membrane-localized transcription factors, each with a periplasmic domain containing two cysteines. In ToxR, these cysteines form an intramolecular disulfide bond...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00338-15
更新日期:2015-11-16 00:00:00
abstract::The halophilic bacterium Chromohalobacter salexigens synthesizes and accumulates compatible solutes in response to salt and temperature stress. (13)C-nuclear magnetic resonance analysis of cells grown in minimal medium at the limiting temperature of 45 degrees C revealed the presence of hydroxyectoine, ectoine, glutam...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00136-06
更新日期:2006-06-01 00:00:00
abstract::soxR governs a superoxide response regulon that contains the genes for endonuclease IV, Mn2(+)-superoxide dismutase, and glucose 6-phosphate dehydrogenase. The soxR gene encodes a 17-kDa protein; some mutations of this gene cause constitutive overexpression of the regulon. Induction by paraquat (methyl viologen) requi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.9.2864-2871.1991
更新日期:1991-05-01 00:00:00
abstract::The frizzy (frz) genes of Myxococcus xanthus are required to control directed motility during vegetative growth and fruiting body formation. FrzCD, a protein homologous to the methyl-accepting chemotaxis proteins from enteric bacteria, is modified by methylation in response to environmental conditions. Transfer of cel...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.15.4936-4940.1993
更新日期:1993-08-01 00:00:00
abstract::Two new mutants of Rhodobacter sphaeroides deficient in sulfolipid accumulation were isolated by directly screening mutagenized cell lines for polar lipid composition by thin-layer chromatography of lipid extracts. A genomic clone which complemented the mutations in these two lines, but not the previously described su...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.20.6479-6487.1992
更新日期:1992-10-01 00:00:00
abstract::Sixteen independent Azorhizobium sesbaniae ORS571 vector insertion (Vi) mutants defective in ammonium assimilation (Asm-) were selected; genomic DNA sequences flanking the insertion endpoints were cloned directly. Resulting recombinant plasmids were used to identify, by hybridization, corresponding wild-type DNA seque...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.3.1197-1204.1988
更新日期:1988-03-01 00:00:00
abstract::TonB-gated transporters have beta-barrels containing an amino-terminal globular domain that occludes the interior of the barrel. Mutations in the globular domain prevent transport of ligands across the outer membrane. Surprisingly, FepA with deletions of the globular domain (amino acids 3 to 150 and 17 to 150) was pre...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.19.5508-5512.2002
更新日期:2002-10-01 00:00:00
abstract::Comamonas testosteroni T-2 uses a standard, if seldom examined, attack on an aromatic compound and oxygenates the side chain of p-toluenesulfonate (TS) (or p-toluenecarboxylate) to p-sulfobenzoate (or terephthalate) prior to complete oxidation. The expression of the first three catabolic enzymes in the pathway, the TS...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.3.919-927.1997
更新日期:1997-02-01 00:00:00
abstract::The large virulence plasmid pMYSH6000 of Shigella flexneri contains a determinant that is highly effective in stabilizing otherwise unstable plasmids in Escherichia coli. Expression of two small contiguous genes, mvpA and mvpT (formerly termed STBORF1 and STBORF2), was shown to be sufficient for stability. Mutations i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.9.2416-2421.2000
更新日期:2000-05-01 00:00:00
abstract::Toxin-antitoxin (TA) systems are widely represented on mobile genetic elements as well as in bacterial chromosomes. TA systems encode a toxin and an antitoxin neutralizing it. We have characterized a homolog of the ccd TA system of the F plasmid (ccd(F)) located in the chromosomal backbone of the pathogenic O157:H7 Es...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01679-06
更新日期:2007-04-01 00:00:00
abstract::Aldrich, K. M. (The University of Kansas, Lawrence), and C. P. Sword. Methicillin-induced lysozyme-sensitive forms of staphylococci. J. Bacteriol. 87:690-695. 1964.-Staphylococcus aureus and S. epidermidis grown in the presence of sublethal amounts of methicillin were converted to enlarged spheres within 2 to 4 hr, as...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.3.690-695.1964
更新日期:1964-03-01 00:00:00
abstract::We used a Southern hybridization assay to locate precisely the sites at which DNA replication is arrested in the terminus region of the Escherichia coli chromosome. The assay was based on the properties of restriction fragments that contain stalled replication forks. Replication forks that entered the terminus from th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.9.4293-4298.1988
更新日期:1988-09-01 00:00:00
abstract::Fox, Eugene N. (University of Chicago, Chicago, Ill.). Intracellular M protein of group A Streptococcus. J. Bacteriol. 85:536-540. 1963.-A heat-labile M protein antigen in protoplasts of a type 14 strain of group A Streptococcus has been demonstrated in a soluble form in the cytoplasm, and also bound to the protoplasm...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.3.536-540.1963
更新日期:1963-03-01 00:00:00
abstract::Nahmias, André J. (Boston University School of Medicine, Boston, Mass.), and Sidney Kibrick. Inhibitory effect of heparin on herpes simplex virus. J. Bacteriol. 87:1060-1066. 1964.-A substance inhibitory to herpes simplex virus was observed during experiments with leukocyte cultures. The component in the cultures resp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.5.1060-1066.1964
更新日期:1964-05-01 00:00:00
abstract::Cellular vanadium metabolism was studied in Saccharomyces cerevisiae by isolating and characterizing vanadate [VO4(3-), V(V)]-resistant mutants. Vanadate growth inhibition was reversed by the removal of the vanadate from the medium, and vanadate resistance was found to be a recessive trait. Vanadate-resistant mutants ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.2.611-617.1985
更新日期:1985-11-01 00:00:00
abstract::Extracellular secretion of Serratia marcescens nuclease occurs as a two-step process via a periplasmic intermediate. Unlike other extracellular proteins secreted by gram-negative bacteria by the general secretory pathway, nuclease accumulates in the periplasm in its active form for an unusually long time before its ex...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.3.677-683.1997
更新日期:1997-02-01 00:00:00
abstract::Both alpha-tocopherolquinol and alpha-tocopherolquinone were found in 56 of 93 strains of microorganisms examined. Organisms that contained these compounds included the single example of a eucaryotic alga, a Euglena, and a cyanobacterium (blue-green alga), 22 of 32 genera of bacteria, and 9 genera of yeasts. In the ba...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.3.1397-1402.1982
更新日期:1982-09-01 00:00:00
abstract::The ribosome content was markedly reduced when illumination (at 28 C) was repeatedly interrupted; an opposite trend was observed with continuous light as the temperature was decreased. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.3.1518-1519.1969
更新日期:1969-03-01 00:00:00
abstract::A series of intercellular signals are involved in the regulation of gene expression during fruiting body formation of Myxococcus xanthus. Mutations which block cell interactions, such as csgA (formerly known as spoC), also prevent expression of certain developmentally regulated promoters. csgA+ cells containing Tn5 la...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.12.5552-5556.1988
更新日期:1988-12-01 00:00:00
abstract::The cysB region in Salmonella typhimurium regulates in a positive manner the noncontiguous structural genes for the enzymes responsible for sulfate reduction in cysteine biosynthesis. We treated three cysB mutants with chemical mutagens and selected 81 secondary mutants in which the inability to utilize sulfate was su...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.1.94-101.1976
更新日期:1976-01-01 00:00:00
abstract::In vitro complementation assays have been used to demonstrate the induction of alkane hydroxylase proteins in mutants lacking the ability to convert n-alkanes to their primary alcohols. Purified heptane is an effective inducer in a mutant lacking detectable hydroxylase activity. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.123.2.759-760.1975
更新日期:1975-08-01 00:00:00
abstract::After inoculation of a stationary-phase culture of Nitrosomonas europaea into fresh growth solution, the cell-associated orthophosphate increased rapidly to 800 mumoles/g (wet weight), whereas the acid-insoluble long-chain polyphosphate content decreased rapidly to 22 mumoles/g. As growth proceeded, the orthophosphate...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.103.1.199-206.1970
更新日期:1970-07-01 00:00:00
abstract::Two key elements that are thought to be required for replication initiation in eubacteria are the DnaA protein, a trans-acting factor, and the replication origin, a cis-acting element. As a first step in studying the replication initiation process in mycobacteria, we have isolated a 4-kb chromosomal DNA fragment from ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.22.6527-6535.1995
更新日期:1995-11-01 00:00:00
abstract::Analysis of the genome sequence of Enterococcus faecalis allowed the identification of two genes whose protein products showed 33 and 34% identity with those of sigV and yrhM of Bacillus subtilis, respectively. These genes, named sigV and rsiV, are predicted to encode members of the extracytoplasmic function subfamily...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.3.1022-1035.2005
更新日期:2005-02-01 00:00:00
abstract::The 50S ribosomal subunits obtained from exponential-phase and valine-limited cultures of Streptococcus faecalis 9790 differed in protein content. Approximately 35% of the valine-limited subunits showed protein losses when analyzed by CsCl density centrifugation after formaldehyde fixation. Results of density gradient...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.1.190-199.1971
更新日期:1971-01-01 00:00:00
abstract::Synthesis of the autoinducer signal involved in the cell density-dependent activation of Vibrio fischeri luminescence is directed by luxI. The autoinducer is N-(3-oxohexanoyl)homoserine lactone, and little is known about its synthesis. We have measured autoinducer synthesis by amino acid auxotrophs of Escherichia coli...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.17.5291-5294.1996
更新日期:1996-09-01 00:00:00
abstract::The ultimate membrane localization and function of most of the 185 predicted Pseudomonas aeruginosa PAO1 lipoproteins remain unknown. We constructed a fluorescent lipoprotein, CSFP(OmlA)-ChFP, by fusing the signal peptide and the first four amino acids of the P. aeruginosa outer membrane lipoprotein OmlA to the monome...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00603-08
更新日期:2008-09-01 00:00:00