Localization of the Escherichia coli rnt gene encoding RNase T by using a combination of physical and genetic mapping.

Abstract:

:The rnt gene encoding RNase T was cloned on a 13-kilobase BamHI fragment. Restriction analysis of the fragment and comparison of it with the Escherichia coli restriction map localized rnt to kilobase coordinates 1733 to 1746, corresponding to about 36 min on the genetic map. The map location was confirmed by cotransduction with the nearby zdg-229::Tn10 and ksgB1 markers.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Case LM,Chen XN,Deutscher MP

doi

10.1128/jb.171.10.5736-5737.1989

subject

Has Abstract

pub_date

1989-10-01 00:00:00

pages

5736-7

issue

10

eissn

0021-9193

issn

1098-5530

journal_volume

171

pub_type

杂志文章
  • Paracrystalline inclusions of a novel ferritin containing nonheme iron, produced by the human gastric pathogen Helicobacter pylori: evidence for a third class of ferritins.

    abstract::An abundant 19.3-kDa Helicobacter pylori protein has been cloned, and the sequence is homologous with a ferritin-like protein produced by Escherichia coli K-12. Homologies are also present with a number of eucaryotic ferritins, as well as with the heme group-containing bacterioferritins. All amino acids involved in ch...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.4.966-972.1993

    authors: Frazier BA,Pfeifer JD,Russell DG,Falk P,Olsén AN,Hammar M,Westblom TU,Normark SJ

    更新日期:1993-02-01 00:00:00

  • Transcriptional regulation of delta-aminolevulinic acid dehydratase synthesis by oxygen in Bradyrhizobium japonicum and evidence for developmental control of the hemB gene.

    abstract::An increased demand for cytochromes is associated with symbiotic development and microaerobic metabolism in the bacterium Bradyrhizobium japonicum, and evidence suggests that hemB, rather than hemA, is the first essential bacterial heme synthesis gene in symbiosis with soybean. Steady-state levels of mRNA and protein ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.11.3706-3710.1997

    authors: Chauhan S,O'Brian MR

    更新日期:1997-06-01 00:00:00

  • DNA supercoiling and suppression of the leu-500 promoter mutation.

    abstract::top mutations (formerly supX) eliminate DNA topoisomerase I activity and suppress the leu-500 promoter mutation in Salmonella typhimurium (K. M. Overbye, S. K. Basu, and P. Margolin, Cold Spring Harbor Symp. Quant. Biol. 47:785-791, 1983). Sublethal doses of coumermycin which reduce intracellular levels of supercoilin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.2.947-949.1985

    authors: Pruss GJ,Drlica K

    更新日期:1985-11-01 00:00:00

  • Importance of anaerobic superoxide dismutase synthesis in facilitating outgrowth of Escherichia coli upon entry into an aerobic habitat.

    abstract::The manganese-containing isozyme of superoxide dismutase (MnSOD) is synthesized by Escherichia coli only during aerobiosis, in accordance with the fact that superoxide can be formed only in aerobic environments. In contrast, E. coli continues to synthesize the iron-containing isozyme (FeSOD) even in the absence of oxy...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.24.7653-7658.1994

    authors: Kargalioglu Y,Imlay JA

    更新日期:1994-12-01 00:00:00

  • Cloning and characterization of two Serratia marcescens genes involved in core lipopolysaccharide biosynthesis.

    abstract::Bacteriocin 28b from Serratia marcescens binds to Escherichia coli outer membrane proteins OmpA and OmpF and to lipopolysaccharide (LPS) core (J. Enfedaque, S. Ferrer, J. F. Guasch, J. Tomás, and M. Requé, Can. J. Microbiol. 42:19-26, 1996). A cosmid-based genomic library of S. marcescens was introduced into E. coli N...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.19.5741-5747.1996

    authors: Guasch JF,Piqué N,Climent N,Ferrer S,Merino S,Rubires X,Tomas JM,Regué M

    更新日期:1996-10-01 00:00:00

  • Properties of D-arabinose isomerase purified from two strains of Escherichia coli.

    abstract::d-Arabinose isomerase (EC 5.3.1.3) has been isolated from l-fucose-induced cultures of Escherichia coli K-12 and d-arabinose-induced cultures of E. coli B/r. Both enzymes were homogeneous in an ultracentrifuge and migrated as single bands upon disc electrophoresis in acrylamide gels. The s(20,w) was 14.5 x 10(-13) sec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.2.687-696.1973

    authors: Boulter JR,Gielow WO

    更新日期:1973-02-01 00:00:00

  • Changes in ribosomal activity of Escherichia coli cells during prolonged culture in sea salts medium.

    abstract::The activity of ribosomes from a clinical isolate of Escherichia coli, exposed to starvation for 7 days in sea salts medium, was investigated by measuring the kinetic parameters of ribosomal peptidyltransferase, by using the puromycin reaction as a model reaction. No alterations in the extent of peptide bond formation...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.12.3114-3119.1998

    authors: Kalpaxis DL,Karahalios P,Papapetropoulou M

    更新日期:1998-06-01 00:00:00

  • Mutations that relieve nutritional repression of the Bacillus subtilis dipeptide permease operon.

    abstract::The Bacillus subtilis dciA operon encodes a dipeptide transport complex that is induced rapidly as cells enter stationary phase and initiate sporulation. Expression of this operon in growing cells is repressed by glucose, by a mixture of amino acids, and by the AbrB protein. A genetic screen was devised to identify mu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.15.4605-4614.1993

    authors: Slack FJ,Mueller JP,Sonenshein AL

    更新日期:1993-08-01 00:00:00

  • Purification and partial characterization of the principal deoxyribonucleic acid polymerase from Mycoplasmatales.

    abstract::In this report we present the first description of the isolation and partial characterization of the deoxyribonucleic acid (DNA) polymerase activity from two species of Mycoplasmatales, Mycoplasma orale type 1 and M. hyorhinis. We have identified only a single DNA polymerase species in the mycoplasma crude extracts, a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.132.2.641-649.1977

    authors: Mills LB,Stanbridge EJ,Sedwick WD,Korn D

    更新日期:1977-11-01 00:00:00

  • Transfection of group H streptococci.

    abstract::Transfection of streptococci is reported here for the first time. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.3.1505-1506.1973

    authors: Parsons CL,Cole RM

    更新日期:1973-03-01 00:00:00

  • Replication of plasmid RK2 in vitro by a DNA-membrane complex: evidence for initiation of replication and its coupling to transcription and translation.

    abstract::The following results with an in vitro replication system utilizing a plasmid RK2 DNA-membrane complex indicate that the essential trfA-encoded replication protein of RK2 is present and active in the complex. (i) A complex extracted from a conditional replication mutant of RK2, which contains a temperature-sensitive m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.167.1.319-326.1986

    authors: Kornacki JA,Firshein W

    更新日期:1986-07-01 00:00:00

  • Mutational analysis of the signal-sensing domain of ResE histidine kinase from Bacillus subtilis.

    abstract::The Bacillus subtilis ResD-ResE two-component regulatory system activates genes involved in nitrate respiration in response to oxygen limitation or nitric oxide (NO). The sensor kinase ResE activates the response regulator ResD through phosphorylation, which then binds to the regulatory region of genes involved in ana...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.6.1694-1704.2004

    authors: Baruah A,Lindsey B,Zhu Y,Nakano MM

    更新日期:2004-03-01 00:00:00

  • Analysis of the dynamics of a Bacillus subtilis spore germination protein complex during spore germination and outgrowth.

    abstract::Germination of Bacillus subtilis spores is normally initiated when nutrients from the environment interact with germinant receptors (GRs) in the spores' inner membrane (IM), in which most of the lipids are immobile. GRs and another germination protein, GerD, colocalize in the IM of dormant spores in a small focus term...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02274-14

    authors: Troiano AJ Jr,Zhang J,Cowan AE,Yu J,Setlow P

    更新日期:2015-01-01 00:00:00

  • Highly repetitive DNA sequences in cyanobacterial genomes.

    abstract::We characterized three distinct families of repeated sequences in the genome of the cyanobacterium Calothrix sp. strain PCC 7601. These repeated sequences were present at a level of about 100 copies per Calothrix genome and consisted of tandemly amplified heptanucleotides. These elements were named short tandemly repe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.5.2755-2761.1990

    authors: Mazel D,Houmard J,Castets AM,Tandeau de Marsac N

    更新日期:1990-05-01 00:00:00

  • Cell division mutations in the blue-green bacterium Agmenellum quadruplicatum strain BG1: a comparison of the cell wall.

    abstract::The peptidoglycan from two types of filamentous cell division mutants of Agmenellum quadruplicatum strain BG1 has been compared to that of the parent organism. Small variations in the total peptidoglycan composition on a dry-weight basis were found in the mutants. The reduced level of peptidoglycan in the serpentine m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.111.2.614-621.1972

    authors: Ingram LO,Van Baalen C,Fisher WD

    更新日期:1972-08-01 00:00:00

  • Relationships between fatty acid and polyketide synthases from Streptomyces coelicolor A3(2): characterization of the fatty acid synthase acyl carrier protein.

    abstract::We have characterized an acyl carrier protein (ACP) presumed to be involved in the synthesis of fatty acids in Streptomyces coelicolor A3(2). This is the third ACP to have been identified in S. coelicolor; the two previously characterized ACPs are involved in the synthesis of two aromatic polyketides: the blue-pigment...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.19.5660-5667.1996

    authors: Revill WP,Bibb MJ,Hopwood DA

    更新日期:1996-10-01 00:00:00

  • Topology of the membrane protein LamB by epitope tagging and a comparison with the X-ray model.

    abstract::We previously developed a genetic approach to study, with a single antibody, the topology of the outer membrane protein LamB, an Escherichia coli porin with specificity towards maltodextrins and a receptor for bacteriophage lambda. Our initial procedure consisted of inserting at random the same reporter epitope (the C...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.12.3447-3456.1996

    authors: Newton SM,Klebba PE,Michel V,Hofnung M,Charbit A

    更新日期:1996-06-01 00:00:00

  • Isolation and preliminary characterization of bacteriophage phi-mu-4.

    abstract::Shafia, Fred (University of Nebraska, Lincoln), and T. L. Thompson. Isolation and preliminary characterization of bacteriophage phimu-4. J. Bacteriol. 87:999-1002. 1964.-Bacteriophage phimu-4 was isolated from lysogenic Bacillus stearothermophilus NU strain 4, and was propagated in strain 10 of the same species. The p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.5.999-1002.1964

    authors: Shafia F,Thompson TL

    更新日期:1964-05-01 00:00:00

  • Isolation and characterization of mutants of colicin plasmids E1 and E2 after Mu bacteriophage infection.

    abstract::Cells colicinogenic for the colicin plasmids E1 or E2 (Col E1 and Col E2, respectively) were selected for a loss of colicin production after infection with bacteriophage Mu. Extrachromosomal deoxyribonucleic acid that was larger than the original colicin plasmids was found in such cells. A small insertion mutant in Co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.119.2.469-477.1974

    authors: Inselburg J

    更新日期:1974-08-01 00:00:00

  • Denitrification by Alcaligenes eutrophus is plasmid dependent.

    abstract::Curing of the hydrogenase-specifying megaplasmid pHG indigenous to strains of the facultative lithoautotrophic bacterium Alcaligenes eutrophus was correlated with a loss of denitrifying ability (Nitd). The retransfer of plasmid pHG1 reconstituted the Nitd phenotype. Plasmid-free mutants were still capable of convertin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.2.852-854.1985

    authors: Römermann D,Friedrich B

    更新日期:1985-05-01 00:00:00

  • Structural and biochemical characterization of the Escherichia coli argE gene product.

    abstract::The DNA sequence of a 2,100-bp region containing the argE gene from Escherichia coli has been determined. The nucleotide sequence of the ppc-argE intergenic region was also solved and shown to contain six tandemly repeated REP sequences. Moreover, the oxyR gene has been mapped on the E. coli chromosome and shown to fl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.7.2323-2331.1992

    authors: Meinnel T,Schmitt E,Mechulam Y,Blanquet S

    更新日期:1992-04-01 00:00:00

  • Characterization of the genes and proteins of a two-component system from the hyperthermophilic bacterium Thermotoga maritima.

    abstract::As a step towards studying representative members of the two-component family of signal transduction proteins, we have cloned genes encoding a histidine protein kinase and a response regulator from the hyperthermophilic bacterium Thermotoga maritima. The genes have been designated HpkA and drrA, respectively. The dedu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.19.5579-5585.1996

    authors: Lee PJ,Stock AM

    更新日期:1996-10-01 00:00:00

  • Toxicity of Pasteurella tularensis killed by ionizing radiation.

    abstract::Approximately 2 x 10(11) viable Pasteurella tularensis cells per ml, contained in suspensions, were killed by exposure to 10(6) r of gamma-radiation. When injected intraperitoneally into mice, the irradiated suspensions initially contained about 10 ld(50) per ml, and immunized mice against challenge with fully virulen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Landay ME,Wright GG,Pulliam JD,Finegold MJ

    更新日期:1968-09-01 00:00:00

  • INTESTINAL BACTERIAL FLORA AND TRANSIT TIME OF THREE NEOTROPICAL BAT SPECIES.

    abstract::Klite, P. D. (Middle America Research Unit, Balboa Heights, Canal Zone). Intestinal bacterial flora and transit time of three neotropical bat species. J. Bacteriol. 90:375-379. 1965.-Quantitative studies on the intestinal bacterial flora of three neotropical bat species revealed the following average bacterial populat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.2.375-379.1965

    authors: KLITE PD

    更新日期:1965-08-01 00:00:00

  • Transcriptional regulation of a promoter in the men gene cluster of Bacillus subtilis.

    abstract::The control of men gene expression during growth and sporulation of Bacillus subtilis was examined at the transcriptional level. Two different approaches were used. (i) Steady-state levels of men-specific mRNA were measured directly. (ii) A men'-lacZ gene fusion was constructed. In both cases, it was observed that men...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.6.2742-2748.1988

    authors: Miller P,Mueller J,Hill K,Taber H

    更新日期:1988-06-01 00:00:00

  • Mutants of Escherichia coli with a growth requirement for either lysine or pyridoxine.

    abstract::Two distinct phenotypic classes of lysine requiring auxotrophs of Escherichia coli are described. Mutants of the LysA class produce little or no active diaminopimelic acid (DAP) decarboxylase and specifically require lysine for growth. Mutants of the LysB class produce a cryptic DAP decarboxylase which can be activate...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.105.3.988-998.1971

    authors: Bukhari AI,Taylor AL

    更新日期:1971-03-01 00:00:00

  • Characterization of the lipopolysaccharide from a Rhizobium phaseoli mutant that is defective in infection thread development.

    abstract::The lipopolysaccharide (LPS) from a Rhizobium phaseoli mutant, CE109, was isolated and compared with that of its wild-type parent, CE3. A previous report has shown that the mutant is defective in infection thread development, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis shows that it has an altered LP...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.11.4923-4928.1987

    authors: Carlson RW,Kalembasa S,Turowski D,Pachori P,Noel KD

    更新日期:1987-11-01 00:00:00

  • Topology analysis of the colicin V export protein CvaA in Escherichia coli.

    abstract::The antibacterial protein toxin colicin V is secreted from Escherichia coli cells by a dedicated export system that is a member of the multicomponent ATP-binding cassette (ABC) transporter family. At least three proteins, CvaA, CvaB, and TolC, are required for secretion via this signal sequence-independent pathway. In...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.21.6153-6159.1995

    authors: Skvirsky RC,Reginald S,Shen X

    更新日期:1995-11-01 00:00:00

  • Inhibitio of flagellar coordination in Spirillum volutans.

    abstract::The motility of Spirillum volutans is caused by the rotation of each polar flagellar fascicle in a direction opposite to that of the more slowly rotating cell. Both flagella form cones of revolution oriented in the same direction. When the cell reverses its motion, both fascicles simultaneously reverse their rotation ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.5.1431-1436.1967

    authors: Krieg NR,Tomelty JP,Wells JS Jr

    更新日期:1967-11-01 00:00:00

  • Regulation of histidine and proline degradation enzymes by amino acid availability in Bacillus subtilis.

    abstract::The first enzymes of the histidine (hut) and proline degradative pathways, histidase and proline oxidase, could not be induced in Bacillus subtilis cells growing in glucose minimal medium containing a mixture of 16 amino acids. Addition of the 16-amino-acid mixture to induced wild-type cells growing in citrate minimal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.9.4758-4765.1990

    authors: Atkinson MR,Wray LV Jr,Fisher SH

    更新日期:1990-09-01 00:00:00