Site-specific integration and expression of a developmental promoter in Myxococcus xanthus.

abstract：:Many of the gene products that participate in nitrogen metabolism are sensitive to nitrogen catabolite repression (NCR), i.e., their expression is decreased to low levels when readily used nitrogen sources such as asparagine are provided. Previous work has shown this NCR sensitivity requires the cis-acting UASNTR elem...

journal_title：Journal of bacteriology

authors： Coffman JA,el Berry HM,Cooper TG

更新日期：1994-12-01 00:00:00

abstract：:PCR-based site-directed mutagenesis has been used to generate 38 alanine-substitution mutations in the C-terminal 41 amino acid residues of LuxR. This region plays a critical role in the mechanism of LuxR-dependent transcriptional activation of the Vibrio fischeri lux operon during quorum sensing. The ability of the v...

journal_title：Journal of bacteriology

authors： Trott AE,Stevens AM

更新日期：2001-01-01 00:00:00

abstract：:The serotype 5 capsule gene cluster of Staphylococcus aureus comprises 16 genes (cap5A through cap5P), but little is known about how the putative gene products function in capsule biosynthesis. We propose that the N-acetylmannosaminuronic acid (ManNAcA) component of the S. aureus serotype 5 capsular polysaccharide (CP...

journal_title：Journal of bacteriology

authors： Kiser KB,Bhasin N,Deng L,Lee JC

更新日期：1999-08-01 00:00:00

abstract：:The antimicrobial metabolite 2,4-diacetylphloroglucinol (2,4-DAPG) contributes to the capacity of Pseudomonas fluorescens strain CHA0 to control plant diseases caused by soilborne pathogens. A 2, 4-DAPG-negative Tn5 insertion mutant of strain CHA0 was isolated, and the nucleotide sequence of the 4-kb genomic DNA regio...

journal_title：Journal of bacteriology

authors： Schnider-Keel U,Seematter A,Maurhofer M,Blumer C,Duffy B,Gigot-Bonnefoy C,Reimmann C,Notz R,Défago G,Haas D,Keel C

更新日期：2000-03-01 00:00:00

abstract：:A class of galactose-resistant mutants has been derived from strains of Salmonella typhimurium which are defective in uridine diphosphoglucose-4-epimerase. Resistant strains are phenotypically similar to parent organisms but do not lyse in the presence of galactose. Low levels of functional epimerase can be detected i...

journal_title：Journal of bacteriology

authors： Shuster CW,Rundell K

更新日期：1969-10-01 00:00:00

abstract：:Bacteriophage 7-7-1, a member of the family Myoviridae, infects the soil bacterium Agrobacterium sp. strain H13-3. Infection requires attachment to actively rotating bacterial flagellar filaments, with flagellar number, length, and rotation speed being important determinants for infection efficiency. To identify the s...

journal_title：Journal of bacteriology

authors： Gonzalez F,Helm RF,Broadway KM,Scharf BE

更新日期：2018-09-10 00:00:00

abstract：:The binding of Bacteroides intermedius VPI 8944 to human fibrinogen has been characterized. The binding is time dependent, at least partially reversible, saturable, and specific. On an average, a maximum of 3,500 fibrinogen molecules bind per bacterial cell, with a dissociation constant of 1.7 X 10(-11) M. These bacte...

journal_title：Journal of bacteriology

authors： Lantz MS,Switalski LM,Kornman KS,Höök M

更新日期：1985-08-01 00:00:00

abstract：:Cyclic di-AMP (c-di-AMP) is an essential and ubiquitous second messenger among bacteria. c-di-AMP regulates many cellular pathways through direct binding to several molecular targets in bacterial cells. c-di-AMP depletion is well known to destabilize the bacterial cell wall, resulting in increased bacteriolysis and en...

journal_title：Journal of bacteriology

authors： Massa SM,Sharma AD,Siletti C,Tu Z,Godfrey JJ,Gutheil WG,Huynh TN

更新日期：2020-11-19 00:00:00

abstract：:Perry, Jerome J. (North Carolina State University, Raleigh), and James B. Evans. Oxidation and assimilation of carbohydrates by Micrococcus sodonensis. J. Bacteriol. 91:33-38. 1966.-Micrococcus sodonensis is a biotin-requiring strict aerobe that cannot utilize carbohydrates as sole sources of carbon and energy. Howeve...

journal_title：Journal of bacteriology

authors： Perry JJ,Evans JB

更新日期：1966-01-01 00:00:00

abstract：:Undecyl acetate esterase has been purified from Pseudomonas cepacia grown on the methyl ketone, 2-tridecanone. The K(m) for undecyl acetate was 2.3 x 10(-2) M. Polyacrylamide gel electrophoresis indicated that two esterase bands were being recovered during purification. These bands were separated by preparative polyac...

journal_title：Journal of bacteriology

authors： Shum AC,Markovetz AJ

更新日期：1974-06-01 00:00:00

abstract：:The sdiA gene codes for a protein that regulates expression of the ftsQAZ cluster of essential cell division genes of Escherichia coli. SdiA up-regulates the ftsQ2p promoter that initiates transcription into the ftsQAZ cluster. In this paper, we report that expression of sdiA is itself regulated by a factor that is re...

journal_title：Journal of bacteriology

authors： García-Lara J,Shang LH,Rothfield LI

更新日期：1996-05-01 00:00:00

abstract：:The widely conserved second messenger cyclic diguanosine monophosphate (c-di-GMP) plays a key role in quorum-sensing (QS)-dependent production of virulence factors in Xanthomonas campestris pv. campestris. The detection of QS diffusible signal factor (DSF) by the sensor RpfC leads to the activation of response regulat...

journal_title：Journal of bacteriology

authors： Tao F,He YW,Wu DH,Swarup S,Zhang LH

更新日期：2010-02-01 00:00:00

abstract：:Unsaturated fatty acids having structural features which are different from those of the monoenoic acids normally synthesized by Escherichia coli can serve as growth factors for an auxotroph requiring unsaturated fatty acids. These analogues were incorporated into the phospholipids, as shown by gas-liquid and thin-lay...

journal_title：Journal of bacteriology

authors： Silbert DF,Ruch F,Vagelos PR

更新日期：1968-05-01 00:00:00

abstract：:In Escherichia coli, the following genes are involved in motility and chemotaxis. The H gene is the structural gene for flagellin. Mutation in the mot gene results in paralysis of the flagella, and mutation in the fla genes leads to an absence of flagella. The cheA, cheB, and cheC genes are required for chemotaxis. Th...

journal_title：Journal of bacteriology

authors： Armstrong JB,Adler J

更新日期：1969-01-01 00:00:00

abstract：:Candida albicans is an opportunistic pathogenic yeast which frequently develops resistance to the antifungal agent fluconazole (FCZ) in patients undergoing long-term therapy. FCZ-resistant strains often display a reduced intracellular FCZ accumulation which correlates with the overexpression of the ATP-binding cassett...

journal_title：Journal of bacteriology

authors： Alarco AM,Raymond M

更新日期：1999-02-01 00:00:00

abstract：:In order to clarify the relationship between polyploidization and the capability of phenotypic switching in the imperfect yeast Candida albicans, two types of variants were isolated as segregants from a fusant, which produced a proportion of the cell population with a higher ploidy than the rest, either in a temperatu...

journal_title：Journal of bacteriology

authors： Suzuki T,Hitomi A,Magee PT,Sakaguchi S

更新日期：1994-06-01 00:00:00

abstract：:Germination and outgrowth of ascospores of Saccharomyces cerevisiae 4579 require both methionine and adenine, whereas leucine is only required for outgrowth. The methionine requirement may be satisfied by S-adenosylmethionine, but this sulfonium compound will not substitute for adenine. Between 30 and 70 min of protei...

journal_title：Journal of bacteriology

authors： Choih SJ,Ferro AJ,Shapiro SK

更新日期：1977-07-01 00:00:00

abstract：:Growth of Streptomyces viridochromogenes on a solid glycerol-NH4NO3 salts medium was accompanied by the formation of aerial mycelia and spores. Adding 0.5% or more casein hydrolysate to the medium stimulated growth while completely repressing the formation of aerial mycelia and spores. This repression was temporary, a...

journal_title：Journal of bacteriology

authors： Coleman RH,Ensign JC

更新日期：1982-03-01 00:00:00

abstract：:A comprehensive equation, upsilon = VM/[1 + (A0.5/Fru-P2)n] [ 1 + (Glc-6-P/I0.5)], has been proposed to represent the quantitative interrelationships between the rate of glucose utilization and the levels of glucose-6-phosphate and fructose-1,6-diphosphate in the intact Escherichia coli cell. This comprehensive equati...

journal_title：Journal of bacteriology

authors： Dietzler DN,Leckie MP,Crimmins DL,Ungar JM,Sternheim WL

更新日期：1976-10-01 00:00:00

abstract：:Mutations in the tcmII-tcmIV region of the Streptomyces glaucescens chromosome block the C-3 and C-8 O-methylations of the polyketide antibiotic tetracenomycin C (Tcm C). The nucleotide sequence of this region reveals the presence of two genes, tcmN and tcmO, whose deduced protein products display similarity to the hy...

journal_title：Journal of bacteriology

authors： Summers RG,Wendt-Pienkowski E,Motamedi H,Hutchinson CR

更新日期：1992-03-01 00:00:00

abstract：:Entry into intestinal epithelial cells is an essential step in the pathogenesis of Salmonella infections. Our laboratory has previously identified a genetic locus, inv, that is necessary for efficient entry of Salmonella typhimurium into cultured epithelial cells. We have carried out a molecular and functional analysi...

journal_title：Journal of bacteriology

authors： Eichelberg K,Ginocchio CC,Galán JE

更新日期：1994-08-01 00:00:00

abstract：:In Escherichia coli, ClpYQ (HslUV) is a two-component ATP-dependent protease composed of ClpY (HslU), an ATPase with unfolding activity, and ClpQ (HslV), a peptidase. In the ClpYQ proteolytic complex, the hexameric rings of ClpY (HslU) are responsible for protein recognition, unfolding, and translocation into the prot...

journal_title：Journal of bacteriology

authors： Hsieh FC,Chen CT,Weng YT,Peng SS,Chen YC,Huang LY,Hu HT,Wu YL,Lin NC,Wu WF

更新日期：2011-10-01 00:00:00

abstract：:In Escherichia coli, the frequencies of IS50 and Tn5 transposition are greater in Dam- cells than in isogenic Dam+ cells. IS50 transposition is increased approximately 1,000-fold and Tn5 transposition frequencies are increased about 5- to 10-fold in the absence of Dam methylation. However, in cells that are deficient ...

journal_title：Journal of bacteriology

authors： Makris JC,Nordmann PL,Reznikoff WS

更新日期：1990-03-01 00:00:00

abstract：:Periplasmic nitrate reductase (NapABC enzyme) has been characterized from a variety of proteobacteria, especially Paracoccus pantotrophus. Whole-genome sequencing of Escherichia coli revealed the structural genes napFDAGHBC, which encode NapABC enzyme and associated electron transfer components. E. coli also expresses...

journal_title：Journal of bacteriology

authors： Stewart V,Lu Y,Darwin AJ

更新日期：2002-03-01 00:00:00

abstract：:Razin, S. (University of Connecticut, Storrs), M. E. Tourtellotte, R. N. McElhaney, and J. D. Pollack. Influence of lipid components of Mycoplasma laidlawii membranes on osmotic fragility of cells. J. Bacteriol. 91:609-616. 1966.-Lipid composition of Mycoplasma laidlawii membranes could be significantly changed by var...

journal_title：Journal of bacteriology

authors： Razin S,Tourtellotte ME,McElhaney RN,Pollack JD

更新日期：1966-02-01 00:00:00

abstract：:The aminophenol (AP) catabolic operon in Pseudomonas putida HS12 mineralizing nitrobenzene was found to contain all the enzymes responsible for the conversion of AP to pyruvate and acetyl coenzyme A via extradiol meta cleavage of 2-aminophenol. The sequence and functional analyses of the corresponding genes of the ope...

journal_title：Journal of bacteriology

authors： Park HS,Kim HS

更新日期：2001-09-01 00:00:00

abstract：:Garrett et al. (Mol. Gen. Genet. 182:326-331, 1981) constructed strains of Escherichia coli harboring derivatives of plasmid pBR322 that carry the lysis genes (S, R, and Rz) of phage lambda. The plasmid construction placed the genes under control of the lactose operon operator-promotor (and thus of lac repressor). Ind...

journal_title：Journal of bacteriology

authors： Crabtree S,Cronan JE Jr

更新日期：1984-04-01 00:00:00

abstract：:Expression plasmids containing various portions of araFGH operon sequences were assayed for their ability to facilitate the high-affinity L-arabinose transport process in a strain lacking the chromosomal copy of this operon. Accumulation studies demonstrated that the specific induction of all three operon coding seque...

journal_title：Journal of bacteriology

authors： Horazdovsky BF,Hogg RW

更新日期：1989-06-01 00:00:00

abstract：:We have developed a general method for determining whether a gene of Escherichia coli is essential for viability. The method requires cloned DNA spanning the gene in question and a reasonably detailed genetic and physical map of the cloned segment. Using this information, one constructs a deletion of the target gene i...

journal_title：Journal of bacteriology

authors： Joyce CM,Grindley ND

更新日期：1984-05-01 00:00:00

abstract：:Enzymes involved in (methyl)phenol degradation of Pseudomonas putida H are encoded by the catabolic operon (phlA-L) on plasmid pPGH1. Transcription of this operon by the sigma54 (RpoN)-containing RNA polymerase is positively controlled by the gene product of the divergently transcribed phlR in response to the availabi...

journal_title：Journal of bacteriology

authors： Müller C,Petruschka L,Cuypers H,Burchhardt G,Herrmann H

更新日期：1996-04-01 00:00:00