Carbon catabolite repression of phenol degradation in Pseudomonas putida is mediated by the inhibition of the activator protein PhlR.


:Enzymes involved in (methyl)phenol degradation of Pseudomonas putida H are encoded by the catabolic operon (phlA-L) on plasmid pPGH1. Transcription of this operon by the sigma54 (RpoN)-containing RNA polymerase is positively controlled by the gene product of the divergently transcribed phlR in response to the availability of the respective substrate. Additionally, phenol degradation is subject to carbon catabolite repression induced by organic acids (e.g., succinate, lactate, and acetate) or carbohydrates (e.g., glucose and gluconate). Analysis of lacZ fusion to the catabolic promoter and quantified primer extension experiments indicate that carbon catabolite repression also occurs at the transcriptional level of the catabolic operon. In this study, it is furthermore shown that carbon catabolite repression is a negative control. Titration of the postulated negative controlling factor was exclusively observed when extra copies of functional phlR gene were present in the cell. We therefore conclude that PhlR is the target and that carbon catabolite repression of phenol degradation occurs by interfering with the activating function of PhlR.


J Bacteriol


Journal of bacteriology


Müller C,Petruschka L,Cuypers H,Burchhardt G,Herrmann H




Has Abstract


1996-04-01 00:00:00












  • Characterization of a human serum inhibitor of Clostridium histolyticum proteinase(s).

    abstract::Normal animal sera inhibit at least one Clostridium histolyticum proteinase. An assay procedure based on immune hemolysis was developed for the estimation of this inhibition. This inhibitory activity occurs in various levels in the sera of different animal species. The highest titers have been obtained with rat sera. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Luzzati A,Goldlust MB,Levine L

    更新日期:1968-12-01 00:00:00

  • Suppression of engulfment defects in bacillus subtilis by elevated expression of the motility regulon.

    abstract::During Bacillus subtilis sporulation, the transient engulfment defect of spoIIB strains is enhanced by spoVG null mutations and suppressed by spoVS null mutations. These mutations have opposite effects on expression of the motility regulon, as the spoVG mutation reduces and the spoVS mutation increases sigmaD-directed...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Perez AR,Abanes-De Mello A,Pogliano K

    更新日期:2006-02-01 00:00:00

  • Cloning and genetic characterization of Helicobacter pylori catalase and construction of a catalase-deficient mutant strain.

    abstract::The N-terminal sequence of a protein, originally described as an adhesin of Helicobacter pylori, was used in an oligonucleotide-based screening procedure of an H. pylori plasmid library in Escherichia coli. Five independent plasmid clones were isolated, all mapping to the same chromosomal region and encoding the H. py...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Odenbreit S,Wieland B,Haas R

    更新日期:1996-12-01 00:00:00

  • Partial purification of a membrane-associated deoxyribonucleic acid complex from Mycoplasma gallisepticum.

    abstract::A Mycoplasma gallisepticum subcellular fraction (P2), which contains the deoxyribonucleic acid replication complex, can be isolated by differential centrifugation of freeze-thaw-lysed cells. The nascent deoxyribonucleic acid is released from P2 by Lubrol-WX, sodium dodecyl sulfate, Pronase, and deoxyribonuclease, but ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Maniloff J,Quinlan DC

    更新日期:1974-10-01 00:00:00

  • Scanning electron microscopy of intact colonies of microorganisms.

    abstract::Colonies of S. mutans OMZ61, Streptococcus sp. D182, Staphylococcus aureus Oxford NCTC 6571, and Candida albicans type A, MRL 3153 were grown on various media. Cubes of agar bearing two to three colonies were excised and processed for scanning electron microscopy. The characteristic shape of the colonies was seen when...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Whittaker DK,Drucker DB

    更新日期:1970-11-01 00:00:00

  • Ultrastructure of paracrystals of a lipoprotein from the outer membrane of Escherichia coli.

    abstract::The highly purified lipoprotein of the outer membrane of Escherichia coli forms paracrystals. The ultrastructures of these paracrystals were examined by electron microscopy. The needle-shaped paracrystals show several different band patterns, depending on conditions of paracrystallization. Models are presented to expl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: DeMartini M,Inouye S,Inouye M

    更新日期:1976-07-01 00:00:00

  • Isolation, phenotypic characterization, and complementation analysis of mutants of Methylobacterium extorquens AM1 unable to synthesize pyrroloquinoline quinone and sequences of pqqD, pqqG, and pqqC.

    abstract::Aerobic gram-negative methylotrophs oxidize methanol to formaldehyde by using a methanol dehydrogenase that has pyrroloquinoline quinone (PQQ) as a prosthetic group. Seventy-two mutants which are unable to grow on methanol unless the growth medium is supplemented with PQQ have been isolated in the facultative methanol...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Morris CJ,Biville F,Turlin E,Lee E,Ellermann K,Fan WH,Ramamoorthi R,Springer AL,Lidstrom ME

    更新日期:1994-03-01 00:00:00

  • Variation in levels of enzymes related to energy metabolism in alternative developmental pathways of Blastocladiella emersonii.

    abstract::The activities of phosphofructokinase (PFK), fructose diphosphatase (FDP), nicotinamide adenine dinucleotide (NAD) and NAD phosphate (NADP)-linked isocitrate dehydrogenases (IDHNAD, IDHNADP), two NAD-linked glutamate dehydrogenases (GDH1, GDH2), and isocitrate lyase were studied during the development of the two pheno...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ingebretsen OC,Sanner T

    更新日期:1976-06-01 00:00:00

  • Infection in mouse peritoneal cavity with a pyrimidine-requiring mutant and naturally occurring Staphylococcus aureus strains.

    abstract::The lethal activity of a thymineless mutant of Staphylococcus aureus Wood 46 strain has been compared with that of three naturally occurring strains: parent Wood 46, Smith, and coagulase-negative SA-13. The thymineless mutant and the parent Wood 46 strain showed a sharp decline in culturable units from the peritoneal ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Mathieu LG,De Repentigny J,Turgeon S,Sonea S

    更新日期:1967-07-01 00:00:00

  • Regulation of breakdown and synthesis of L-glutamate decarboxylase in Clostridium perfringens.

    abstract::L-Glutamate decarboxylase (GAD) activity of Clostridium perfringens (ATCC 8009) cells grown in various culture conditions was investigated. Remarkable variations of GAD level occur during the growth cycle in thioglycollate broth. These changes are affected by the pH of the culture medium. Addition of alkali to the cul...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Cozzani I,Barsacchi R,Dibenedetto G,Saracchi L,Falcone G

    更新日期:1975-09-01 00:00:00

  • Composition of the fractions separated by polyacrylamide gel electrophoresis of the lipopolysaccharide of a marine bacterium.

    abstract::The sugar composition of lipopolysaccharide (LPS) isolated from whole cells of Alteromonas haloplanktis 214 (previously referred to as marine pseudomonas B-16, ATCC 19855), variant 3, of the lipid A, core, and side-chain fractions derived from it, and of the LPS fractions (LPS I, II, and III) obtained by subjecting it...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: DiRienzo JM,MacLeod RA

    更新日期:1978-10-01 00:00:00

  • Analysis of dofA, a fruA-dependent developmental gene, and its homologue, dofB, in Myxococcus xanthus.

    abstract::The developmentally regulated gene dofA, identified from pulse-labeling experiments by two-dimensional gel electrophoresis, and its homologue, dofB, were cloned and characterized in Myxococcus xanthus. Deletion of dofA and dofB did not affect the vegetative growth and development of M. xanthus. dofA was specifically e...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Horiuchi T,Akiyama T,Inouye S,Komano T

    更新日期:2002-12-01 00:00:00

  • Two-component response regulators of Vibrio fischeri: identification, mutagenesis, and characterization.

    abstract::Two-component signal transduction systems are utilized by prokaryotic and eukaryotic cells to sense and respond to environmental stimuli, both to maintain homeostasis and to rapidly adapt to changing conditions. Studies have begun to emerge that utilize a large-scale mutagenesis approach to analyzing these systems in ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hussa EA,O'Shea TM,Darnell CL,Ruby EG,Visick KL

    更新日期:2007-08-01 00:00:00

  • Compartmentalization of the periplasmic space at division sites in gram-negative bacteria.

    abstract::Phase-contrast and serial-section electron microscopy were used to study the patterns of localized plasmolysis that occur when cells of Salmonella typhimurium and Escherichia coli are exposed to hypertonic solutions of sucrose. In dividing cells the nascent septum was flanked by localized regions of periseptal plasmol...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Cook WR,MacAlister TJ,Rothfield LI

    更新日期:1986-12-01 00:00:00

  • The bile response repressor BreR regulates expression of the Vibrio cholerae breAB efflux system operon.

    abstract::Enteric pathogens have developed several resistance mechanisms to survive the antimicrobial action of bile. We investigated the transcriptional profile of Vibrio cholerae O1 El Tor strain C6706 under virulence gene-inducing conditions in the presence and absence of bile. Microarray analysis revealed that the expressio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Cerda-Maira FA,Ringelberg CS,Taylor RK

    更新日期:2008-11-01 00:00:00

  • Promoter recognition and activation by the global response regulator CbrB in Pseudomonas aeruginosa.

    abstract::In Pseudomonas aeruginosa, the CbrA/CbrB two-component system is instrumental in the maintenance of the carbon-nitrogen balance and for growth on carbon sources that are energetically less favorable than the preferred dicarboxylate substrates. The CbrA/CbrB system drives the expression of the small RNA CrcZ, which ant...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Abdou L,Chou HT,Haas D,Lu CD

    更新日期:2011-06-01 00:00:00

  • Transcription of spontaneously released bacterial deoxyribonucleic acid in frog auricles.

    abstract::After frog auricles have been in contact with a suspension of bacteria or bacteria-free supernatant fluid, newly synthesized bacterial ribonucleic acid (RNA) is recovered in animal cells. It appears that the presence of bacterial deoxyribonucleic acid (DNA)-dependent RNA polymerase is necessary for the transcription o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Stroun M,Anker P

    更新日期:1973-04-01 00:00:00

  • Comparison of the two isofunctional enol-lactone hydrolases from Acinetobacter calcoaceticus.

    abstract::The rates of thermal denaturation and the molecular weights of the two isofunctional enol-lactone hydrolases (ELH I and ELH II) of Acinetobacter calcoaceticus were determined. The molecular weights of ELH I and ELH II were found by gel filtration to be approximately 24,000 and 21,000, respectively. In crude extract at...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Katagiri M,Wheelis ML

    更新日期:1971-05-01 00:00:00

  • A new family of integral membrane proteins involved in transport of aromatic amino acids in Escherichia coli.

    abstract::The nucleotide sequence of tnaB of the tryptophanase operon of Escherichia coli is presented. TnaB is a tryptophan-specific permease that is homologous to Mtr, a second tryptophan-specific permease, and to TyrP, a tyrosine-specific permease. Each member of this family appears to contain 11 membrane-spanning domains. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sarsero JP,Wookey PJ,Gollnick P,Yanofsky C,Pittard AJ

    更新日期:1991-05-01 00:00:00

  • Genetic rearrangement associated with in vivo mucoid conversion of Pseudomonas aeruginosa PAO is due to insertion elements.

    abstract::The conversion of Pseudomonas aeruginosa PAO to the mucoid phenotype has been reported for a chronic pulmonary infection model in rats (D. E. Woods, P. A. Sokol, L. E. Bryan, D. G. Storey, S. J. Mattingly, H. J. Vogel, and H. Ceri, J. Infect. Dis. 163:143-149, 1991). This conversion was associated with a genetic rearr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sokol PA,Luan MZ,Storey DG,Thirukkumaran P

    更新日期:1994-02-01 00:00:00

  • Regulation of the SOS response analyzed by RecA protein amplification.

    abstract::A split UV light dose procedure was used in Escherichia coli to induce an SOS function, RecA protein amplification, which was measured by an immunoradiometric assay. The SOS system was partially induced after the first UV irradiation, and the inducing effects of subsequent identical UV doses were quantified. Variation...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Calsou P,Salles B

    更新日期:1985-06-01 00:00:00

  • The C terminus of sigma(32) is not essential for degradation by FtsH.

    abstract::A key step in the regulation of heat shock genes in Escherichia coli is the stress-dependent degradation of the heat shock promoter-specific sigma(32) subunit of RNA polymerase by the AAA protease, FtsH. Previous studies implicated the C termini of protein substrates, including sigma(32), as degradation signals for AA...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Tomoyasu T,Arsène F,Ogura T,Bukau B

    更新日期:2001-10-01 00:00:00

  • The Proline Variant of the W[F/L/M][T/S]R Cyclic Di-GMP Binding Motif Suppresses Dependence on Signal Association for Regulator Function.

    abstract::Vibrio vulnificus is an estuarine bacterium and potent opportunistic human pathogen. It enters the food chain by asymptomatically colonizing a variety of marine organisms, most notably oysters. Expression of the brp-encoded extracellular polysaccharide, which enhances cell-surface adherence, is regulated by cyclic di-...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Chodur DM,Guo L,Pu M,Bruger E,Fernandez N,Waters C,Rowe-Magnus DA

    更新日期:2017-09-05 00:00:00

  • Genetic analysis of bacteriophage lambda cIII gene: mRNA structural requirements for translation initiation.

    abstract::The bacteriophage lambda cIII gene product regulates the lysogenic pathway. The cIII gene is located in the leftward operon, which is transcribed from the pL promoter. We have previously shown (S. Altuvia and A. B. Oppenheim, J. Bacteriol. 167:415-419, 1986) that mutations that show elevated expression lie within the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kornitzer D,Teff D,Altuvia S,Oppenheim AB

    更新日期:1989-05-01 00:00:00

  • Visualization of ribosomal ribonucleic acid synthesis in a ribonuclease III-Deficient strain of Escherichia coli.

    abstract::Transmission electron microscopy was used to examine active ribosomal ribonucleic acid (rRNA) genes in two strains of Escherichia coli: N2077, deficient in the enzyme responsible for proper cleavage of the 16S sequence from the elongating nascent rRNA transcript; and N2076, functional in ribonuclease (RNase) III activ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hofmann S,Miller OL Jr

    更新日期:1977-11-01 00:00:00

  • Under Elevated c-di-GMP in Escherichia coli, YcgR Alters Flagellar Motor Bias and Speed Sequentially, with Additional Negative Control of the Flagellar Regulon via the Adaptor Protein RssB.

    abstract::In Escherichia coli and Salmonella, the c-di-GMP effector YcgR inhibits flagellar motility by interacting directly with the motor to alter both its bias and speed. Here, we demonstrate that in both of these bacteria, YcgR acts sequentially, altering motor bias first and then decreasing motor speed. We show that when c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Nieto V,Partridge JD,Severin GB,Lai RZ,Waters CM,Parkinson JS,Harshey RM

    更新日期:2019-12-06 00:00:00

  • Autoinduction of 2,4-diacetylphloroglucinol biosynthesis in the biocontrol agent Pseudomonas fluorescens CHA0 and repression by the bacterial metabolites salicylate and pyoluteorin.

    abstract::The antimicrobial metabolite 2,4-diacetylphloroglucinol (2,4-DAPG) contributes to the capacity of Pseudomonas fluorescens strain CHA0 to control plant diseases caused by soilborne pathogens. A 2, 4-DAPG-negative Tn5 insertion mutant of strain CHA0 was isolated, and the nucleotide sequence of the 4-kb genomic DNA regio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Schnider-Keel U,Seematter A,Maurhofer M,Blumer C,Duffy B,Gigot-Bonnefoy C,Reimmann C,Notz R,Défago G,Haas D,Keel C

    更新日期:2000-03-01 00:00:00

  • Ultraviolet sensitivity of Bacillus subtilis spores upon germination and outgrowth.

    abstract::A strain of Bacillus subtilis, UVSSP-42-1, which produces ultraviolet (UV)-sensitive spores and vegetative cells, was found to possess germinated spores 25 times more UV resistant than the resting spores. This relative resistance achieved upon germination was associated with the transition of the heat-resistant refrac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Munakata N

    更新日期:1974-10-01 00:00:00

  • The Rnf Complex Is an Energy-Coupled Transhydrogenase Essential To Reversibly Link Cellular NADH and Ferredoxin Pools in the Acetogen Acetobacterium woodii.

    abstract::The Rnf complex is a respiratory enzyme that catalyzes the oxidation of reduced ferredoxin to the reduction of NAD+, and the negative free energy change of this reaction is used to generate a transmembrane ion gradient. In one class of anaerobic acetogenic bacteria, the Rnf complex is believed to be essential for ener...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Westphal L,Wiechmann A,Baker J,Minton NP,Müller V

    更新日期:2018-10-10 00:00:00

  • Purification and characterization of S-adenosylhomocysteine deaminase from streptonigrin-producing Streptomyces flocculus.

    abstract::An S-adenosylhomocysteine deaminase has been isolated and purified from streptonigrin-producing Streptomyces flocculus ATCC 13257. Deamination represents the major metabolic route of S-adenosylhomocysteine in this organism. The protein was found to be monomeric with a molecular weight of 56,100 +/- 1,600. The activity...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Zulty JJ,Speedie MK

    更新日期:1989-12-01 00:00:00