Abstract:
:Enzymes involved in (methyl)phenol degradation of Pseudomonas putida H are encoded by the catabolic operon (phlA-L) on plasmid pPGH1. Transcription of this operon by the sigma54 (RpoN)-containing RNA polymerase is positively controlled by the gene product of the divergently transcribed phlR in response to the availability of the respective substrate. Additionally, phenol degradation is subject to carbon catabolite repression induced by organic acids (e.g., succinate, lactate, and acetate) or carbohydrates (e.g., glucose and gluconate). Analysis of lacZ fusion to the catabolic promoter and quantified primer extension experiments indicate that carbon catabolite repression also occurs at the transcriptional level of the catabolic operon. In this study, it is furthermore shown that carbon catabolite repression is a negative control. Titration of the postulated negative controlling factor was exclusively observed when extra copies of functional phlR gene were present in the cell. We therefore conclude that PhlR is the target and that carbon catabolite repression of phenol degradation occurs by interfering with the activating function of PhlR.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Müller C,Petruschka L,Cuypers H,Burchhardt G,Herrmann Hdoi
10.1128/jb.178.7.2030-2036.1996subject
Has Abstractpub_date
1996-04-01 00:00:00pages
2030-6issue
7eissn
0021-9193issn
1098-5530journal_volume
178pub_type
杂志文章abstract::RasP is a predicted intramembrane metalloprotease of Bacillus subtilis that has been proposed to cleave the stress response anti-sigma factors RsiW and RsiV, the cell division protein FtsL, and remnant signal peptides within their transmembrane segments. To provide evidence for direct effects of RasP on putative subst...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00381-17
更新日期:2017-09-05 00:00:00
abstract::SaPIbov2 is a member of the SaPI family of staphylococcal pathogenicity islands and is very closely related to SaPIbov1. Typically, certain temperate phages can induce excision and replication of one or more of these islands and can package them into special small phage-like particles commensurate with their genome si...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00619-07
更新日期:2007-08-01 00:00:00
abstract::Chloramphenicol-resistant strains of Staphylococcus aureus contain an inducible enzyme which inactivates chloramphenicol by acetylation in the presence of acetyl coenzyme A. The products of acetylation are chromatographically indistinguishable from those obtained with chloramphenicol-resistant Escherichia coli harbori...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.1.28-36.1968
更新日期:1968-01-01 00:00:00
abstract::Frampton, E. W. (The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston), and B. R. Brinkley. Evidence of lysogeny in derivatives of Escherichia coli. J. Bacteriol. 90:446-452. 1965.-Exposure of cultures of several thymine-requiring derivatives of Escherichia coli strain 15 to radiation [X rays o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.2.446-452.1965
更新日期:1965-08-01 00:00:00
abstract::Band 7 proteins, which encompass members of the stomatin, prohibitin, flotillin, and HflK/C protein families, are integral membrane proteins that play important physiological roles in eukaryotes but are poorly characterized in bacteria. We have studied the band 7 proteins encoded by the cyanobacterium Synechocystis sp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00644-09
更新日期:2009-10-01 00:00:00
abstract::A gene which complements a paralyzed flagellar mutant of Rhodobacter sphaeroides was sequenced. The derived protein sequence has similarity to MotB. R. sphaeroides MotB lacks the C-terminal peptidoglycan-binding motif of other MotB proteins. This divergence of sequence may reflect the unusual, unidirectional, stop-sta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.10.2929-2932.1995
更新日期:1995-05-01 00:00:00
abstract::Translational efficiency of an AUG, CUG, GUG, or UUG initiation codon was measured for the naturally leaderless cI mRNA from bacteriophage lambda. In a cI-lacZ translational fusion, only AUG supported a high level of expression; GUG supported a low level of expression, while UUG and CUG expression was barely above bac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.4.1277-1283.2001
更新日期:2001-02-01 00:00:00
abstract::A method has been developed for studying the specific activity of the pool of S-adenosylmethionine in yeast. The pool reaches half-maximal specific activity within 30 s after the addition of [methyl-3H]methionine. After addition of an excess of nonradioactive methionine, the specific activity of S-adenosylmethionine i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.3.887-891.1976
更新日期:1976-03-01 00:00:00
abstract::Resting cell suspensions of Neisseria meningitidis group B (strain 2091) do not catabolize citrate as the sole substrate to an appreciable degree. When another substrate, such as glutamate, is also present to furnish energy for transport, citrate metabolism is greatly stimulated. Within limits, the amount of CO(2) pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.106.3.819-823.1971
更新日期:1971-06-01 00:00:00
abstract::spo0A and spo0H are needed for the initiation of sporulation and for the development of genetic competence in Bacillus subtilis. Transcription of spo0A initiates from two promoters, Pv and Ps. Pv is active during vegetative growth and is recognized by RNA polymerase containing sigma A. Expression from Ps increases dur...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.12.3812-3815.1994
更新日期:1994-06-01 00:00:00
abstract::When dl-isoleucine was added to an ammonium nitrate-maltose medium during cultivation of Streptomyces sp. 732, quinomycin B formation was selectively enhanced (from 3 to 70% of the quinomycin mixture) and quinomycin C synthesis was inhibited completely. In addition, two new quinomycins, designated quinomycins D and E,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.4.1327-1331.1967
更新日期:1967-04-01 00:00:00
abstract::The DnaK protein of Zymomonas mobilis (DnaKz) was identified and found to be 80% identical to the DnaK protein of Escherichia coli on the basis of the sequence of the N-terminal 21 amino acids. The dnaKz gene was cloned and found to be expressed in a thermosensitive dnaK mutant of Escherichia coli. Expression of the f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.10.3228-3231.1993
更新日期:1993-05-01 00:00:00
abstract::The mechanism of action of diphtheria toxin in an Escherichia coli cell-free protein-synthesizing system was examined. When the washed ribosomes were incubated with toxin before addition of messenger ribonucleic acid (RNA), peptide syntheses of (14)C-phenylalanine directed by polyuridylic acid or phage R17 RNA were st...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.1.152-157.1970
更新日期:1970-10-01 00:00:00
abstract::We previously elucidated the global transcriptional responses of Escherichia coli to the nitrosating agent S-nitrosoglutathione (GSNO) in both aerobic and anaerobic chemostats, demonstrated the expression of nitric oxide (NO)-protective mechanisms, and obtained evidence of critical thiol nitrosation. The present study...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01354-06
更新日期:2007-03-01 00:00:00
abstract::We previously showed that adsorption of bacteriophages T4 and T5 to their respective outer membrane receptors induced a partial depolarization of the cytoplasmic membrane. As these membrane potential changes were independent of phage properties, we proposed that phage adsorption triggered the emission of a signal whic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.3.789-794.1984
更新日期:1984-03-01 00:00:00
abstract::Production of deoxyribonucleotides for DNA synthesis is an essential and tightly regulated process. The class Ia ribonucleotide reductase (RNR), the product of the nrdAB genes, is required for aerobic growth of Escherichia coli. In catalyzing the reduction of ribonucleotides, two of the cysteines of RNR become oxidize...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05989-11
更新日期:2012-03-01 00:00:00
abstract::The guanine plus cytosine (GC) content of the deoxyribonucleic acid (DNA) of 11 isolates of host-dependent (H-D) bdellovibrios and 18 host-independent (H-I) derivatives was determined from thermal denaturation curves and buoyant densities in CsCl. The H-D and respective H-I cultures have GC contents which are identica...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.2.786-790.1969
更新日期:1969-11-01 00:00:00
abstract::The Escherichia coli purine repressor, PurR, binds to a 16-bp operator sequence and coregulates the genes for de novo synthesis of purine and pyrimidine nucleotides, formation of a one-carbon unit for biosynthesis, and deamination of cytosine. We have characterized the purified repressor. Chemical cross-linking indica...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.19.6207-6214.1992
更新日期:1992-10-01 00:00:00
abstract::RpoS is a conserved alternative sigma factor that regulates the expression of many stress response genes in Escherichia coli. The RpoS regulon is large but has not yet been completely characterized. In this study, we report the identification of over 100 RpoS-dependent fusions in a genetic screen based on the differen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.24.8499-8507.2004
更新日期:2004-12-01 00:00:00
abstract::The antigenically related coaggregation receptor polysaccharides (RPS) of Streptococcus oralis strains C104 and SK144 mediate recognition of these bacteria by other members of the dental plaque biofilm community. In the present study, the structure of strain SK144 RPS was established by high resolution NMR spectroscop...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01532-08
更新日期:2009-03-01 00:00:00
abstract::Inositol derivative compounds provide a nutrient source for soil bacteria that possess the ability to degrade such compounds. Rhizobium strains that are capable of utilizing certain inositol derivatives are better colonizers of their host plants. We have cloned and determined the nucleotide sequence of the myo-inosito...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.8.2595-2604.2001
更新日期:2001-04-01 00:00:00
abstract::Cell-free extracts of Bacillus licheniformis and B. cereus were found to contain high specific activities of nicotinamide adenine dinucleotide phosphate (NADP)-dependent-l-glutamate dehydrogenase [EC 1.4.1.4; l-glutamate: NADP oxidoreductase (deaminating)]. Maximum specific activities were found in extracts of cells d...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.106.2.375-385.1971
更新日期:1971-05-01 00:00:00
abstract::Small nuclear ribonucleoprotein (snRNP) particles play an important role in the processing of pre-mRNA. snRNPs have been identified immunologically in a variety of cells, but none have ever been observed in prokaryotic systems. This report provides the first evidence for the presence of snRNP-like components in two ty...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.7.1871-1878.1993
更新日期:1993-04-01 00:00:00
abstract::We have identified IS100 sequences in a specific subset of Yersinia pseudotuberculosis isolates that were also sensitive to the Y. pestis-produced bacteriocin, pesticin. In contrast, Y. pseudotuberculosis strains which did not contain IS100 sequences were not sensitive to pesticin. We propose that IS100 serves as a mo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.6.2081-2085.1997
更新日期:1997-03-01 00:00:00
abstract::Expression of the lysine decarboxylase gene (cadA) of Escherichia coli is induced upon external acidification. To dissect the molecular mechanisms responsible for this regulation, we analyzed a 4.2-kbp region upstream from cadA. DNA sequencing revealed two long open reading frames upstream of and on the same strand as...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.2.530-540.1992
更新日期:1992-01-01 00:00:00
abstract::von Graevenitz, Alexander (Yale University, New Haven, Conn.). Genetic transfer of streptomycin and aminopropanolstreptomycin resistance. J. Bacteriol. 88:960-964. 1964.-Transduction and conjugation experiments with Staphylococcus aureus and Escherichia coli suggest that resistance to streptomycin (S) and aminopropano...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.4.960-964.1964
更新日期:1964-10-01 00:00:00
abstract::The plasmid-encoded epidermin biosynthetic gene epiC of Staphylococcus epidermidis Tü3298 was expressed in Escherichia coli by using the T7 RNA polymerase-promoter system, and the gene product EpiC was identified by Western blotting (immunoblotting) with an anti-EpiC-peptide antiserum. EpiC was a hydrophobic but solub...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.5.1335-1340.1996
更新日期:1996-03-01 00:00:00
abstract::Guanosine tetraphosphate (ppGpp) is a key mediator of stringent control, an adaptive response of bacteria to amino acid starvation, and has thus been termed a bacterial alarmone. Previous X-ray crystallographic analysis has provided a structural basis for the transcriptional regulation of RNA polymerase activity by pp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00574-06
更新日期:2006-10-01 00:00:00
abstract::Catabolic mannopine (MOP) cyclase encoded by certain Agrobacterium Ti and Ri plasmids lactonizes MOP to agropine (AGR). The enzyme, purified to homogeneity from a recombinant clone, has a molecular mass of 45 kDa as measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and size exclusion chromatography...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.8.2427-2430.1996
更新日期:1996-04-01 00:00:00
abstract::Daniel, John W. (University of Wisconsin, Madison), Karlee L. Babcock, Alice H. Sievert, and Harold P. Rusch. Organic requirements and synthetic media for growth of the myxomycete Physarum polycephalum. J. Bacteriol. 86:324-331. 1963.-An isolate of Physarum polycephalum was maintained in submersed pure culture in a co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.2.324-331.1963
更新日期:1963-08-01 00:00:00