Carbon catabolite repression of phenol degradation in Pseudomonas putida is mediated by the inhibition of the activator protein PhlR.

Abstract:

:Enzymes involved in (methyl)phenol degradation of Pseudomonas putida H are encoded by the catabolic operon (phlA-L) on plasmid pPGH1. Transcription of this operon by the sigma54 (RpoN)-containing RNA polymerase is positively controlled by the gene product of the divergently transcribed phlR in response to the availability of the respective substrate. Additionally, phenol degradation is subject to carbon catabolite repression induced by organic acids (e.g., succinate, lactate, and acetate) or carbohydrates (e.g., glucose and gluconate). Analysis of lacZ fusion to the catabolic promoter and quantified primer extension experiments indicate that carbon catabolite repression also occurs at the transcriptional level of the catabolic operon. In this study, it is furthermore shown that carbon catabolite repression is a negative control. Titration of the postulated negative controlling factor was exclusively observed when extra copies of functional phlR gene were present in the cell. We therefore conclude that PhlR is the target and that carbon catabolite repression of phenol degradation occurs by interfering with the activating function of PhlR.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Müller C,Petruschka L,Cuypers H,Burchhardt G,Herrmann H

doi

10.1128/jb.178.7.2030-2036.1996

subject

Has Abstract

pub_date

1996-04-01 00:00:00

pages

2030-6

issue

7

eissn

0021-9193

issn

1098-5530

journal_volume

178

pub_type

杂志文章
  • Bacillus subtilis Intramembrane Protease RasP Activity in Escherichia coli and In Vitro.

    abstract::RasP is a predicted intramembrane metalloprotease of Bacillus subtilis that has been proposed to cleave the stress response anti-sigma factors RsiW and RsiV, the cell division protein FtsL, and remnant signal peptides within their transmembrane segments. To provide evidence for direct effects of RasP on putative subst...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00381-17

    authors: Parrell D,Zhang Y,Olenic S,Kroos L

    更新日期:2017-09-05 00:00:00

  • Role of staphylococcal phage and SaPI integrase in intra- and interspecies SaPI transfer.

    abstract::SaPIbov2 is a member of the SaPI family of staphylococcal pathogenicity islands and is very closely related to SaPIbov1. Typically, certain temperate phages can induce excision and replication of one or more of these islands and can package them into special small phage-like particles commensurate with their genome si...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00619-07

    authors: Maiques E,Ubeda C,Tormo MA,Ferrer MD,Lasa I,Novick RP,Penadés JR

    更新日期:2007-08-01 00:00:00

  • Characterization of chloramphenicol acetyltransferase from chloramphenicol-resistant Staphylococcus aureus.

    abstract::Chloramphenicol-resistant strains of Staphylococcus aureus contain an inducible enzyme which inactivates chloramphenicol by acetylation in the presence of acetyl coenzyme A. The products of acetylation are chromatographically indistinguishable from those obtained with chloramphenicol-resistant Escherichia coli harbori...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.1.28-36.1968

    authors: Shaw WV,Brodsky RF

    更新日期:1968-01-01 00:00:00

  • EVIDENCE OF LYSOGENY IN DERIVATIVES OF ESCHERICHIA COLI.

    abstract::Frampton, E. W. (The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston), and B. R. Brinkley. Evidence of lysogeny in derivatives of Escherichia coli. J. Bacteriol. 90:446-452. 1965.-Exposure of cultures of several thymine-requiring derivatives of Escherichia coli strain 15 to radiation [X rays o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.2.446-452.1965

    authors: FRAMPTON EW,BRINKLEY BR

    更新日期:1965-08-01 00:00:00

  • Structural and mutational analysis of band 7 proteins in the cyanobacterium Synechocystis sp. strain PCC 6803.

    abstract::Band 7 proteins, which encompass members of the stomatin, prohibitin, flotillin, and HflK/C protein families, are integral membrane proteins that play important physiological roles in eukaryotes but are poorly characterized in bacteria. We have studied the band 7 proteins encoded by the cyanobacterium Synechocystis sp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00644-09

    authors: Boehm M,Nield J,Zhang P,Aro EM,Komenda J,Nixon PJ

    更新日期:2009-10-01 00:00:00

  • Rhodobacter sphaeroides WS8 expresses a polypeptide that is similar to MotB of Escherichia coli.

    abstract::A gene which complements a paralyzed flagellar mutant of Rhodobacter sphaeroides was sequenced. The derived protein sequence has similarity to MotB. R. sphaeroides MotB lacks the C-terminal peptidoglycan-binding motif of other MotB proteins. This divergence of sequence may reflect the unusual, unidirectional, stop-sta...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.10.2929-2932.1995

    authors: Shah DS,Armitage JP,Sockett RE

    更新日期:1995-05-01 00:00:00

  • The initiation codon affects ribosome binding and translational efficiency in Escherichia coli of cI mRNA with or without the 5' untranslated leader.

    abstract::Translational efficiency of an AUG, CUG, GUG, or UUG initiation codon was measured for the naturally leaderless cI mRNA from bacteriophage lambda. In a cI-lacZ translational fusion, only AUG supported a high level of expression; GUG supported a low level of expression, while UUG and CUG expression was barely above bac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.4.1277-1283.2001

    authors: O'Donnell SM,Janssen GR

    更新日期:2001-02-01 00:00:00

  • Kinetics of labeling of the S-adenosylmethionine pool of Saccharomyces cerevisiae.

    abstract::A method has been developed for studying the specific activity of the pool of S-adenosylmethionine in yeast. The pool reaches half-maximal specific activity within 30 s after the addition of [methyl-3H]methionine. After addition of an excess of nonradioactive methionine, the specific activity of S-adenosylmethionine i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.125.3.887-891.1976

    authors: Warner JR,Morgan SA,Shulman RW

    更新日期:1976-03-01 00:00:00

  • Effect of glutamate on exogenous citrate catabolism of Neisseria meningitidis and of other species of Neisseria.

    abstract::Resting cell suspensions of Neisseria meningitidis group B (strain 2091) do not catabolize citrate as the sole substrate to an appreciable degree. When another substrate, such as glutamate, is also present to furnish energy for transport, citrate metabolism is greatly stimulated. Within limits, the amount of CO(2) pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.106.3.819-823.1971

    authors: Hill JC

    更新日期:1971-06-01 00:00:00

  • Activation of spo0A transcription by sigma H is necessary for sporulation but not for competence in Bacillus subtilis.

    abstract::spo0A and spo0H are needed for the initiation of sporulation and for the development of genetic competence in Bacillus subtilis. Transcription of spo0A initiates from two promoters, Pv and Ps. Pv is active during vegetative growth and is recognized by RNA polymerase containing sigma A. Expression from Ps increases dur...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.12.3812-3815.1994

    authors: Siranosian KJ,Grossman AD

    更新日期:1994-06-01 00:00:00

  • Influence of isoleucine upon quinomycin biosynthesis by Streptomyces sp. 732.

    abstract::When dl-isoleucine was added to an ammonium nitrate-maltose medium during cultivation of Streptomyces sp. 732, quinomycin B formation was selectively enhanced (from 3 to 70% of the quinomycin mixture) and quinomycin C synthesis was inhibited completely. In addition, two new quinomycins, designated quinomycins D and E,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.4.1327-1331.1967

    authors: Yoshida T,Katagiri K

    更新日期:1967-04-01 00:00:00

  • Cloning and expression in Escherichia coli of the dnaK gene of Zymomonas mobilis.

    abstract::The DnaK protein of Zymomonas mobilis (DnaKz) was identified and found to be 80% identical to the DnaK protein of Escherichia coli on the basis of the sequence of the N-terminal 21 amino acids. The dnaKz gene was cloned and found to be expressed in a thermosensitive dnaK mutant of Escherichia coli. Expression of the f...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.10.3228-3231.1993

    authors: Michel GP

    更新日期:1993-05-01 00:00:00

  • Inhibitory effect of diphtheria toxin on amino acid incorporation in Escherichia coli cell-free system.

    abstract::The mechanism of action of diphtheria toxin in an Escherichia coli cell-free protein-synthesizing system was examined. When the washed ribosomes were incubated with toxin before addition of messenger ribonucleic acid (RNA), peptide syntheses of (14)C-phenylalanine directed by polyuridylic acid or phage R17 RNA were st...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.1.152-157.1970

    authors: Tsugawa A,Ohsumi Y,Kato I

    更新日期:1970-10-01 00:00:00

  • Nitric oxide in chemostat-cultured Escherichia coli is sensed by Fnr and other global regulators: unaltered methionine biosynthesis indicates lack of S nitrosation.

    abstract::We previously elucidated the global transcriptional responses of Escherichia coli to the nitrosating agent S-nitrosoglutathione (GSNO) in both aerobic and anaerobic chemostats, demonstrated the expression of nitric oxide (NO)-protective mechanisms, and obtained evidence of critical thiol nitrosation. The present study...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01354-06

    authors: Pullan ST,Gidley MD,Jones RA,Barrett J,Stevanin TM,Read RC,Green J,Poole RK

    更新日期:2007-03-01 00:00:00

  • Involvement of envelope-bound calcium in the transient depolarization of the Escherichia coli cytoplasmic membrane induced by bacteriophage T4 and T5 adsorption.

    abstract::We previously showed that adsorption of bacteriophages T4 and T5 to their respective outer membrane receptors induced a partial depolarization of the cytoplasmic membrane. As these membrane potential changes were independent of phage properties, we proposed that phage adsorption triggered the emission of a signal whic...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.157.3.789-794.1984

    authors: Letellier L,Labedan B

    更新日期:1984-03-01 00:00:00

  • Mutations at several loci cause increased expression of ribonucleotide reductase in Escherichia coli.

    abstract::Production of deoxyribonucleotides for DNA synthesis is an essential and tightly regulated process. The class Ia ribonucleotide reductase (RNR), the product of the nrdAB genes, is required for aerobic growth of Escherichia coli. In catalyzing the reduction of ribonucleotides, two of the cysteines of RNR become oxidize...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05989-11

    authors: Feeney MA,Ke N,Beckwith J

    更新日期:2012-03-01 00:00:00

  • Deoxyribonucleic acid characterization of Bdellovibrios.

    abstract::The guanine plus cytosine (GC) content of the deoxyribonucleic acid (DNA) of 11 isolates of host-dependent (H-D) bdellovibrios and 18 host-independent (H-I) derivatives was determined from thermal denaturation curves and buoyant densities in CsCl. The H-D and respective H-I cultures have GC contents which are identica...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.2.786-790.1969

    authors: Seidler RJ,Starr MP,Mandel M

    更新日期:1969-11-01 00:00:00

  • Structural characterization and corepressor binding of the Escherichia coli purine repressor.

    abstract::The Escherichia coli purine repressor, PurR, binds to a 16-bp operator sequence and coregulates the genes for de novo synthesis of purine and pyrimidine nucleotides, formation of a one-carbon unit for biosynthesis, and deamination of cytosine. We have characterized the purified repressor. Chemical cross-linking indica...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.19.6207-6214.1992

    authors: Choi KY,Zalkin H

    更新日期:1992-10-01 00:00:00

  • RpoS-regulated genes of Escherichia coli identified by random lacZ fusion mutagenesis.

    abstract::RpoS is a conserved alternative sigma factor that regulates the expression of many stress response genes in Escherichia coli. The RpoS regulon is large but has not yet been completely characterized. In this study, we report the identification of over 100 RpoS-dependent fusions in a genetic screen based on the differen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.24.8499-8507.2004

    authors: Vijayakumar SR,Kirchhof MG,Patten CL,Schellhorn HE

    更新日期:2004-12-01 00:00:00

  • Comparative structural and molecular characterization of ribitol-5-phosphate-containing Streptococcus oralis coaggregation receptor polysaccharides.

    abstract::The antigenically related coaggregation receptor polysaccharides (RPS) of Streptococcus oralis strains C104 and SK144 mediate recognition of these bacteria by other members of the dental plaque biofilm community. In the present study, the structure of strain SK144 RPS was established by high resolution NMR spectroscop...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01532-08

    authors: Yang J,Ritchey M,Yoshida Y,Bush CA,Cisar JO

    更新日期:2009-03-01 00:00:00

  • A functional myo-inositol dehydrogenase gene is required for efficient nitrogen fixation and competitiveness of Sinorhizobium fredii USDA191 to nodulate soybean (Glycine max [L.] Merr.).

    abstract::Inositol derivative compounds provide a nutrient source for soil bacteria that possess the ability to degrade such compounds. Rhizobium strains that are capable of utilizing certain inositol derivatives are better colonizers of their host plants. We have cloned and determined the nucleotide sequence of the myo-inosito...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.8.2595-2604.2001

    authors: Jiang G,Krishnan AH,Kim YW,Wacek TJ,Krishnan HB

    更新日期:2001-04-01 00:00:00

  • Purification, properties, and regulation of glutamic dehydrogenase of Bacillus licheniformis.

    abstract::Cell-free extracts of Bacillus licheniformis and B. cereus were found to contain high specific activities of nicotinamide adenine dinucleotide phosphate (NADP)-dependent-l-glutamate dehydrogenase [EC 1.4.1.4; l-glutamate: NADP oxidoreductase (deaminating)]. Maximum specific activities were found in extracts of cells d...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.106.2.375-385.1971

    authors: Phibbs PV Jr,Bernlohr RW

    更新日期:1971-05-01 00:00:00

  • Eubacterial components similar to small nuclear ribonucleoproteins: identification of immunoprecipitable proteins and capped RNAs in a cyanobacterium and a gram-positive eubacterium.

    abstract::Small nuclear ribonucleoprotein (snRNP) particles play an important role in the processing of pre-mRNA. snRNPs have been identified immunologically in a variety of cells, but none have ever been observed in prokaryotic systems. This report provides the first evidence for the presence of snRNP-like components in two ty...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.7.1871-1878.1993

    authors: Kovacs SA,O'Neil J,Watcharapijarn J,Moe-Kirvan C,Vijay S,Silva V

    更新日期:1993-04-01 00:00:00

  • Homology with a repeated Yersinia pestis DNA sequence IS100 correlates with pesticin sensitivity in Yersinia pseudotuberculosis.

    abstract::We have identified IS100 sequences in a specific subset of Yersinia pseudotuberculosis isolates that were also sensitive to the Y. pestis-produced bacteriocin, pesticin. In contrast, Y. pseudotuberculosis strains which did not contain IS100 sequences were not sensitive to pesticin. We propose that IS100 serves as a mo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.6.2081-2085.1997

    authors: McDonough KA,Hare JM

    更新日期:1997-03-01 00:00:00

  • Identification of elements involved in transcriptional regulation of the Escherichia coli cad operon by external pH.

    abstract::Expression of the lysine decarboxylase gene (cadA) of Escherichia coli is induced upon external acidification. To dissect the molecular mechanisms responsible for this regulation, we analyzed a 4.2-kbp region upstream from cadA. DNA sequencing revealed two long open reading frames upstream of and on the same strand as...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.2.530-540.1992

    authors: Watson N,Dunyak DS,Rosey EL,Slonczewski JL,Olson ER

    更新日期:1992-01-01 00:00:00

  • GENETIC TRANSFER OF STREPTOMYCIN AND AMINOPROPANOLSTREPTOMYCIN RESISTANCE.

    abstract::von Graevenitz, Alexander (Yale University, New Haven, Conn.). Genetic transfer of streptomycin and aminopropanolstreptomycin resistance. J. Bacteriol. 88:960-964. 1964.-Transduction and conjugation experiments with Staphylococcus aureus and Escherichia coli suggest that resistance to streptomycin (S) and aminopropano...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.4.960-964.1964

    authors: VONGRAEVENITZ A

    更新日期:1964-10-01 00:00:00

  • Expression, purification, and characterization of EpiC, an enzyme involved in the biosynthesis of the lantibiotic epidermin, and sequence analysis of Staphylococcus epidermidis epiC mutants.

    abstract::The plasmid-encoded epidermin biosynthetic gene epiC of Staphylococcus epidermidis Tü3298 was expressed in Escherichia coli by using the T7 RNA polymerase-promoter system, and the gene product EpiC was identified by Western blotting (immunoblotting) with an anti-EpiC-peptide antiserum. EpiC was a hydrophobic but solub...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.5.1335-1340.1996

    authors: Kupke T,Gotz F

    更新日期:1996-03-01 00:00:00

  • Physiological analysis of the stringent response elicited in an extreme thermophilic bacterium, Thermus thermophilus.

    abstract::Guanosine tetraphosphate (ppGpp) is a key mediator of stringent control, an adaptive response of bacteria to amino acid starvation, and has thus been termed a bacterial alarmone. Previous X-ray crystallographic analysis has provided a structural basis for the transcriptional regulation of RNA polymerase activity by pp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00574-06

    authors: Kasai K,Nishizawa T,Takahashi K,Hosaka T,Aoki H,Ochi K

    更新日期:2006-10-01 00:00:00

  • Purification and characterization of catabolic mannopine cyclase encoded by the Agrobacterium tumefaciens Ti plasmid pTi15955.

    abstract::Catabolic mannopine (MOP) cyclase encoded by certain Agrobacterium Ti and Ri plasmids lactonizes MOP to agropine (AGR). The enzyme, purified to homogeneity from a recombinant clone, has a molecular mass of 45 kDa as measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and size exclusion chromatography...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.8.2427-2430.1996

    authors: Hong SB,Farrand SK

    更新日期:1996-04-01 00:00:00

  • ORGANIC REQUIREMENTS AND SYNTHETIC MEDIA FOR GROWTH OF THE MYXOMYCETE PHYSARUM POLYCEPHALUM.

    abstract::Daniel, John W. (University of Wisconsin, Madison), Karlee L. Babcock, Alice H. Sievert, and Harold P. Rusch. Organic requirements and synthetic media for growth of the myxomycete Physarum polycephalum. J. Bacteriol. 86:324-331. 1963.-An isolate of Physarum polycephalum was maintained in submersed pure culture in a co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.86.2.324-331.1963

    authors: DANIEL JW,BABCOCK KL,SIEVERT AH,RUSCH HP

    更新日期:1963-08-01 00:00:00