Carbon catabolite repression of phenol degradation in Pseudomonas putida is mediated by the inhibition of the activator protein PhlR.


:Enzymes involved in (methyl)phenol degradation of Pseudomonas putida H are encoded by the catabolic operon (phlA-L) on plasmid pPGH1. Transcription of this operon by the sigma54 (RpoN)-containing RNA polymerase is positively controlled by the gene product of the divergently transcribed phlR in response to the availability of the respective substrate. Additionally, phenol degradation is subject to carbon catabolite repression induced by organic acids (e.g., succinate, lactate, and acetate) or carbohydrates (e.g., glucose and gluconate). Analysis of lacZ fusion to the catabolic promoter and quantified primer extension experiments indicate that carbon catabolite repression also occurs at the transcriptional level of the catabolic operon. In this study, it is furthermore shown that carbon catabolite repression is a negative control. Titration of the postulated negative controlling factor was exclusively observed when extra copies of functional phlR gene were present in the cell. We therefore conclude that PhlR is the target and that carbon catabolite repression of phenol degradation occurs by interfering with the activating function of PhlR.


J Bacteriol


Journal of bacteriology


Müller C,Petruschka L,Cuypers H,Burchhardt G,Herrmann H




Has Abstract


1996-04-01 00:00:00












  • Characterization of the primary immunity region of the Escherichia coli linear plasmid prophage N15.

    abstract::N15 is the only bacteriophage of Escherichia coli known to lysogenize as a linear plasmid. Clear-plaque mutations lie in at least two regions of the 46-kb genome. We have cloned, sequenced, and characterized the primary immunity region, immB. This region contains a gene, cB, whose product shows homology to lambdoid ph...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lobocka MB,Svarchevsky AN,Rybchin VN,Yarmolinsky MB

    更新日期:1996-05-01 00:00:00

  • Characterization of a highly structured domain in Tbp2 from Neisseria meningitidis involved in binding to human transferrin.

    abstract::The binding of iron-loaded human transferrin at the surface of Neisseria meningitidis is mediated by two polypeptides, Tbp1 and Tbp2. Predicted Tbp amino acid sequences from N. meningitidis strains are highly divergent. This variability is particularly pronounced throughout the Tbp2 polypeptide. In this study, a highl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Vonder Haar RA,Legrain M,Kolbe HV,Jacobs E

    更新日期:1994-10-01 00:00:00

  • The plant pathogen Pantoea ananatis produces N-acylhomoserine lactone and causes center rot disease of onion by quorum sensing.

    abstract::A number of gram-negative bacteria have a quorum-sensing system and produce N-acyl-l-homoserine lactone (AHL) that they use them as a quorum-sensing signal molecule. Pantoea ananatis is reported as a common colonist of wheat heads at ripening and causes center rot of onion. In this study, we demonstrated that P. anana...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Morohoshi T,Nakamura Y,Yamazaki G,Ishida A,Kato N,Ikeda T

    更新日期:2007-11-01 00:00:00

  • Nucleotide sequence of the papA gene encoding the Pap pilus subunit of human uropathogenic Escherichia coli.

    abstract::The papA gene of the uropathogenic strain Escherichia coli J96, coding for the Pap pili subunit, was subjected to DNA sequencing, and found to code for an 185-amino acid-long polypeptide with a 22-amino acid-long signal peptide. Here we present the primary sequence, the hydrophilicity profile, and the predicted polype...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Båga M,Normark S,Hardy J,O'Hanley P,Lark D,Olsson O,Schoolnik G,Falkow S

    更新日期:1984-01-01 00:00:00

  • Positive selection for loss-of-function tat mutations identifies critical residues required for TatA activity.

    abstract::The Tat system, found in the cytoplasmic membrane of many bacteria, is a general export pathway for folded proteins. Here we describe the development of a method, based on the transport of chloramphenicol acetyltransferase, that allows positive selection of mutants defective in Tat function. We have demonstrated the u...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hicks MG,Lee PA,Georgiou G,Berks BC,Palmer T

    更新日期:2005-04-01 00:00:00

  • Control of morphogenesis in Arthrobacter crystallopoiets: effect of cyclic adenosine 3',5'-monophosphate.

    abstract::The intracellular levels of cyclic adenosine 3',5'-monophosphate (cyclic AMP) were measured at various intervals during growth and morphogenesis in Arthrobacter crystallopoietes. Cyclic AMP levels remained relatively constant throughout growth in spherical cells grown in glucose-based media. Immediately after inoculat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hamilton RW,Achberger EC,Kolenbrander PE

    更新日期:1977-02-01 00:00:00

  • Suppression of an Hsp70 mutant phenotype in Saccharomyces cerevisiae through loss of function of the chromatin component Sin1p/Spt2p.

    abstract::The Ssa subfamily of Hsp70 molecular chaperones in the budding yeast Saccharomyces cerevisiae has four members, encoded by SSA1, SSA2, SSA3, and SSA4. Deletion of the two constitutively expressed genes, SSA1 and SSA2, results in cells which are slow growing and temperature sensitive. In this study, we demonstrate that...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Baxter BK,Craig EA

    更新日期:1998-12-01 00:00:00

  • Phosphorylation of lipopolysaccharides in the Antarctic psychrotroph Pseudomonas syringae: a possible role in temperature adaptation.

    abstract::Phosphorylation of lipopolysaccharide (LPS) from a psychrotrophic bacterium, Pseudomonas syringae, from Antarctica was studied by using sucrose gradient-separated membrane fractions. The bacterium was found to possess an LPS kinase which could phosphorylate more LPS postsynthetically at higher temperatures. The phosph...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ray MK,Kumar GS,Shivaji S

    更新日期:1994-07-01 00:00:00

  • Sortase-catalyzed assembly of distinct heteromeric fimbriae in Actinomyces naeslundii.

    abstract::Two types of adhesive fimbriae are expressed by Actinomyces; however, the architecture and the mechanism of assembly of these structures remain poorly understood. In this study we characterized two fimbrial gene clusters present in the genome of Actinomyces naeslundii strain MG-1. By using immunoelectron microscopy an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Mishra A,Das A,Cisar JO,Ton-That H

    更新日期:2007-04-01 00:00:00

  • Chemotaxis toward amino acids in Escherichia coli.

    abstract::Escherichia coli cells are shown to be attracted to the l-amino acids alanine, asparagine, aspartate, cysteine, glutamate, glycine, methionine, serine, and threonine, but not to arginine, cystine, glutamine, histidine, isoleucine, leucine, lysine, phenylalanine, tryptophan, tyrosine, or valine. Bacteria grown in a pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Mesibov R,Adler J

    更新日期:1972-10-01 00:00:00

  • Polyphosphate and orthophosphate content of Nitrosomonas europaea as a function of growth.

    abstract::After inoculation of a stationary-phase culture of Nitrosomonas europaea into fresh growth solution, the cell-associated orthophosphate increased rapidly to 800 mumoles/g (wet weight), whereas the acid-insoluble long-chain polyphosphate content decreased rapidly to 22 mumoles/g. As growth proceeded, the orthophosphate...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Terry KR,Hooper AB

    更新日期:1970-07-01 00:00:00

  • Expression of Adhesive Pili and the Collagen-Binding Adhesin Ace Is Activated by ArgR Family Transcription Factors in Enterococcus faecalis.

    abstract::It was shown previously that the disruption of the ahrC gene encoding a predicted ArgR family transcription factor results in a severe defect in biofilm formation in vitro, as well as a significant attenuation of virulence of Enterococcus faecalis strain OG1RF in multiple experimental infection models. Using transcrip...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Manias DA,Dunny GM

    更新日期:2018-08-24 00:00:00

  • Factors affecting transformation of Micrococcus lysodeikticus.

    abstract::Relatively high transfo mation frequencies were obtained with Micrococcus lysodeikticus cells, previou[ill]sly grown in defined broth, with a tris(hydroxymethyl)-aminomethane-glutamate b uffer containing Mg(++), Ca(++), Ba(++), or Sr(++). ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kloos WE

    更新日期:1969-06-01 00:00:00

  • Electron microscopy of single-stranded structures in the DNA of competent Haemophilus influenzae cells.

    abstract::Chromosomal DNAs from exponential-phase and competent cells of Haemophilus influenzae were examined by electron microscopy to determine whether the chromosome undergoes structural changes during competence development. Single-stranded gaps and single-stranded tails formed in chromosomal DNA during competence developme...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: McCarthy D,Kupfer DM

    更新日期:1987-02-01 00:00:00

  • FepA with globular domain deletions lacks activity.

    abstract::TonB-gated transporters have beta-barrels containing an amino-terminal globular domain that occludes the interior of the barrel. Mutations in the globular domain prevent transport of ligands across the outer membrane. Surprisingly, FepA with deletions of the globular domain (amino acids 3 to 150 and 17 to 150) was pre...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Vakharia HL,Postle K

    更新日期:2002-10-01 00:00:00

  • Tricistronic operon expression of the genes gcaD (tms), which encodes N-acetylglucosamine 1-phosphate uridyltransferase, prs, which encodes phosphoribosyl diphosphate synthetase, and ctc in vegetative cells of Bacillus subtilis.

    abstract::The gcaD, prs, and ctc genes were shown to be organized as a tricistronic operon. The transcription of the prs gene, measured as phosphoribosyl diphosphate synthetase activity, and of the ctc gene, measured as beta-galactosidase activity specified by a ctc-lacZ protein fusion, were dependent on the promoter in front o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hilden I,Krath BN,Hove-Jensen B

    更新日期:1995-12-01 00:00:00

  • Release of respiratory control in Escherichia coli after bacteriophage adsorption: process independent of DNA injection.

    abstract::Adsorption of phages T4, T5, and BF23 to previously starved Escherichia coli cells triggered the immediate release of respiratory control. A similar stimulation of respiration was induced after T4 ghost attachment, showing that this process was independent of the mechanism of DNA injection. Rather, this change in the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Letellier L,Labedan B

    更新日期:1985-01-01 00:00:00

  • KtrAB, a new type of bacterial K(+)-uptake system from Vibrio alginolyticus.

    abstract::Vibrio alginolyticus contained two adjacent genes, ktrA and ktrB, which encode a new type of bacterial K(+)-uptake system. KtrA and KtrB are peripheral and integral membrane proteins, respectively. Six of the nine sequenced bacterial genomes contain homologs to both ktrA and ktrB, suggesting that KtrAB is widespread. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Nakamura T,Yuda R,Unemoto T,Bakker EP

    更新日期:1998-07-01 00:00:00

  • RNase III processing of intervening sequences found in helix 9 of 23S rRNA in the alpha subclass of Proteobacteria.

    abstract::We provide experimental evidence for RNase III-dependent processing in helix 9 of the 23S rRNA as a general feature of many species in the alpha subclass of Proteobacteria (alpha-Proteobacteria). We investigated 12 Rhodobacter, Rhizobium, Sinorhizobium, Rhodopseudomonas, and Bartonella strains. The processed region is...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Evguenieva-Hackenberg E,Klug G

    更新日期:2000-09-01 00:00:00

  • Selection of succinic dehydrogenase mutants of Neurospora crassa.

    abstract::A method is described which permits the selection of mutants of Neurospora crassa that are deficient in succinic dehydrogenase activity. The method relies on the observation that succinic dehydrogenase-deficient strains fail to reduce the dye nitrotetrazolium blue when overlaid with the dye in the presence of succinat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Edwards DL,Belsole DM,Guzik HJ,Unger BW

    更新日期:1979-02-01 00:00:00

  • A Ti plasmid-encoded enzyme required for degradation of mannopine is functionally homologous to the T-region-encoded enzyme required for synthesis of this opine in crown gall tumors.

    abstract::The mocC gene encoded by the octopine/mannityl opine-type Ti plasmid pTi15955 is related at the nucleotide sequence level to mas1' encoded by the T region of this plasmid. While Mas1 is required for the synthesis of mannopine (MOP) by crown gall tumor cells, MocC is essential for the utilization of MOP by Agrobacteriu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kim KS,Chilton WS,Farrand SK

    更新日期:1996-06-01 00:00:00


    abstract::Banerjee, Arun B. (University of Calcutta, Calcutta, India), and S. K. Bose. Biosynthesis of mycobacillin, a new antifungal peptide. II. Relation between streptomycin dependence and biosynthesis. J. Bacteriol. 87:1402-1405. 1964.-A streptomycin-dependent variant was isolated by a single-step mutation process from a st...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: BANERJEE AB,BOSE SK

    更新日期:1964-06-01 00:00:00

  • Structure and Membrane Topography of the Vibrio-Type Secretin Complex from the Type 2 Secretion System of Enteropathogenic Escherichia coli.

    abstract::The β-barrel assembly machinery (BAM) complex is the core machinery for the assembly of β-barrel membrane proteins, and inhibition of BAM complex activity is lethal to bacteria. Discovery of integral membrane proteins that are key to pathogenesis and yet do not require assistance from the BAM complex raises the questi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hay ID,Belousoff MJ,Dunstan RA,Bamert RS,Lithgow T

    更新日期:2018-02-07 00:00:00

  • Timing of ribosome synthesis during ascosporogenesis of yeast cells: evidence for early function of haploid daughter genomes.

    abstract::During meiosis and sporulation in Saccharomyces cerevisiae, the recessive genetic marker for cycloheximide resistance, believed to be due to an altered ribosomal protein (C. S. McLaughlin, p. 815-827, in M. Nomura et al., ed., Ribosomes, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.), is expressed as early a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Emanuel JR,Magee PT

    更新日期:1981-03-01 00:00:00

  • Fibronectin binding to the Salmonella enterica serotype Typhimurium ShdA autotransporter protein is inhibited by a monoclonal antibody recognizing the A3 repeat.

    abstract::ShdA is a large outer membrane protein of the autotransporter family whose passenger domain binds the extracellular matrix proteins fibronectin and collagen I, possibly by mimicking the host ligand heparin. The ShdA passenger domain consists of approximately 1,500 amino acid residues that can be divided into two regio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kingsley RA,Abi Ghanem D,Puebla-Osorio N,Keestra AM,Berghman L,Bäumler AJ

    更新日期:2004-08-01 00:00:00

  • Transcript analysis of multiple copies of amo (encoding ammonia monooxygenase) and hao (encoding hydroxylamine oxidoreductase) in Nitrosomonas europaea.

    abstract::The genes encoding ammonia monooxygenase (amoCAB), hydroxylamine oxidoreductase (hao), and the c-type cytochrome c-554 (hcy) are present in multiple copies in the genome of Nitrosomonas europaea. The upstream regions of the two copies of amoC, the three copies of hao, and one copy of hcy were cloned and sequenced. Pri...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hommes NG,Sayavedra-Soto LA,Arp DJ

    更新日期:2001-02-01 00:00:00

  • Effects of essential amino acid starvation in Streptococcus faecalis: structural change in the 50S ribosomal subunit.

    abstract::The 50S ribosomal subunits obtained from exponential-phase and valine-limited cultures of Streptococcus faecalis 9790 differed in protein content. Approximately 35% of the valine-limited subunits showed protein losses when analyzed by CsCl density centrifugation after formaldehyde fixation. Results of density gradient...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ziegler RJ,Daneo-Moore L

    更新日期:1971-01-01 00:00:00

  • Aspartate transcarbamylase synthesis ceases prior to inactivation of the enzyme in Bacillus subtilis.

    abstract::Aspartate transcarbamylase is synthesized during exponential growth of Bacillus subtilis and is inactivated when the cells enter the stationary phase. This work is a study of the regulation of aspartate transcarbamylase synthesis during growth and the stationary phase. Using specific immunoprecipitation of aspartate t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Maurizi MR,Switzer RL

    更新日期:1978-09-01 00:00:00

  • Identification and characterization of the nifV-nifZ-nifT gene region from the filamentous cyanobacterium Anabaena sp. strain PCC 7120.

    abstract::The nifV and leuA genes, which encode homocitrate synthase and alpha-isopropylmalate synthase, respectively, were cloned from the filamentous cyanobacterium Anabaena sp. strain PCC 7120 by a PCR-based strategy. Since the N-terminal parts of NifV and LeuA from other bacteria are highly similar to each other, a single p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Stricker O,Masepohl B,Klipp W,Böhme H

    更新日期:1997-05-01 00:00:00

  • Lysozyme-promoted association of protein I molecules in the outer membrane of Escherichia coli.

    abstract::Incubation of whole envelopes prepared from sonically oscillated Escherichia coli K-12 cultures with lysozyme in vitro resulted in the appearance of a protein species with an apparent molecular weight double that of outer membrane protein I. Similar dimers were also detected in purified outer membranes and whole envel...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Chopra I,Howe GB,Ball PR

    更新日期:1977-11-01 00:00:00