Specific inhibition of mature fungal serine proteinases and metalloproteinases by their propeptides.

abstract：:The ToxR protein of Vibrio cholerae regulates the expression of several virulence factors that play important roles in the pathogenesis of cholera. Previous experiments with ToxR-alkaline phosphatase (ToxR-PhoA) fusion proteins suggested a model for gene regulation in which the inactive form of ToxR was a monomer and ...

journal_title：Journal of bacteriology

authors： Ottemann KM,Mekalanos JJ

更新日期：1996-01-01 00:00:00

abstract：:Studies of mitochondrial biogenesis in yeast have been hampered by a lack of suitable membrane markers in anaerobically grown cells subsequently grown in air. Cytochrome c peroxidase activity and subcellular location was studied to determine whether it would be a useful marker for an analysis of mitochondrial formatio...

journal_title：Journal of bacteriology

authors： Avers CJ

更新日期：1967-10-01 00:00:00

abstract：:A 22-kb segment of chromosomal DNA from Enterococcus faecalis OG1RF containing the pyrimidine biosynthesis genes pyrC and pyrD was previously detected as complementing Escherichia coli pyrC and pyrD mutations. In the present study, it was found that the E. faecalis pyrimidine biosynthetic genes in this clone (designat...

journal_title：Journal of bacteriology

authors： Li X,Weinstock GM,Murray BE

更新日期：1995-12-01 00:00:00

abstract：:Campylobacter jejuni, a spiral-shaped gram-negative bacterium, is a leading bacterial cause of human food-borne illness. Acute disease is associated with C. jejuni invasion of the intestinal epithelium. Further, maximal host cell invasion requires the secretion of proteins termed Campylobacter invasion antigens (Cia)....

journal_title：Journal of bacteriology

authors： Malik-Kale P,Parker CT,Konkel ME

更新日期：2008-04-01 00:00:00

abstract：:The alpha-aminoadipate pathway for lysine biosynthesis is present only in fungi. The alpha-aminoadipate reductase (AAR) of this pathway catalyzes the conversion of alpha-aminoadipic acid to alpha-aminoadipic-delta-semialdehyde by a complex mechanism involving two gene products, Lys2p and Lys5p. The LYS2 and LYS5 genes...

journal_title：Journal of bacteriology

authors： Guo S,Evans SA,Wilkes MB,Bhattacharjee JK

更新日期：2001-12-01 00:00:00

abstract：:A population of Bacillus subtilis can be fractionated into at least two components on the basis of their buoyant densities on a Renografin gradient. In competent cultures, most, and perhaps all, of the competent bacteria appear in the lighter fraction which composes 2 to 10% of the bulk population. These "lighter" bac...

journal_title：Journal of bacteriology

authors： Cahn FH,Fox MS

更新日期：1968-03-01 00:00:00

abstract：:Bacterial flagellar filaments grow at their distal ends, from flagellin that travels through a central channel ∼2 nm in diameter. The flagellin is extruded from the cytoplasm by a pump powered by a proton motive force (PMF). We measured filament growth in cells near the mid-exponential-phase with flagellin bearing a s...

journal_title：Journal of bacteriology

authors： Turner L,Stern AS,Berg HC

更新日期：2012-05-01 00:00:00

abstract：:We have identified recD mutants of Salmonella typhimurium by their ability to support growth of phage P22 abc (anti-RecBCD) mutants, whose growth is prevented by normal host RecBCD function. As in Escherichia coli, the recD gene of S. typhimurium lies between the recB and argA genes at min 61 of the genetic map. Plasm...

journal_title：Journal of bacteriology

authors： Miesel L,Roth JR

更新日期：1994-07-01 00:00:00

abstract：:Controlled acetolysis of yeast mannans yields mixtures of oligosaccharides with (1-->2) and (1-->3) linkages between the mannose units, whereas the less stable (1-->6) linkages of the polysaccharide backbone are cleaved. The "fingerprints," obtained by gel filtration of the oligosaccharide mixtures, can be used to dis...

journal_title：Journal of bacteriology

authors： Kocourek J,Ballou CE

更新日期：1969-12-01 00:00:00

abstract：:The degradation of synthetic compounds requires bacteria to recruit and adapt enzymes from pathways for naturally occurring compounds. Previous work defined the steps in 2,4-dinitrotoluene (2,4-DNT) metabolism through the ring fission reaction. The results presented here characterize subsequent steps in the pathway th...

journal_title：Journal of bacteriology

authors： Johnson GR,Jain RK,Spain JC

更新日期：2002-08-01 00:00:00

abstract：:In Acinetobacter calcoaceticus the seven genes coding for the enzymes responsible for tryptophan synthesis map at three chromosomal locations. Two three-gene clusters, one (trpGDC) specifying the small subunit of anthranilate synthase, phosphoribosyl transferase, and indoleglycerol phosphate synthase and the other (tr...

journal_title：Journal of bacteriology

authors： Cohn W,Crawford IP

更新日期：1976-07-01 00:00:00

abstract：:Competent cell-deoxyribonucleic acid (DNA) interactions were examined using tritium-labeled homologous or heterologous native or denatured DNAs and competent Streptococcus sanguis Wicky cells (strain WE4). The DNAs used were extracted from WE4 cells, Escherichia coli B cells, and E. coli bacteriophages T2, T4, T6, and...

journal_title：Journal of bacteriology

authors： Ranhand JM

更新日期：1980-05-01 00:00:00

abstract：:We have investigated the evolutionary relationships between two facultatively anaerobic Magnetospirillum strains (AMB-1 and MGT-1) and fastidious, obligately microaerophilic species, such as Magnetospirillum magnetotacticum, using a molecular phylogenetic approach. Genomic DNA from strains MGT-1 and AMB-1 was used as ...

journal_title：Journal of bacteriology

authors： Burgess JG,Kawaguchi R,Sakaguchi T,Thornhill RH,Matsunaga T

更新日期：1993-10-01 00:00:00

abstract：:The conjugally acquired deoxyribonucleic acid (DNA) of small, anucleate cells ("minicells") of a mutant strain of Escherichia coli K-12 was found to be predominantly associated with the bacterial membrane. Evidence from X-irradiation studies in vivo shows that there is no decrease in DNA-membrane association under con...

journal_title：Journal of bacteriology

authors： Shull FW Jr,Fralick JA,Stratton LP,Fisher WD

更新日期：1971-05-01 00:00:00

abstract：:The expression of any given bacterial protein is predicted to depend on (i) the transcriptional regulation of the promoter and the translational regulation of its mRNA and (ii) the synthesis and translation of total (bulk) mRNA. This is because total mRNA acts as a competitor to the specific mRNA for the binding of in...

journal_title：Journal of bacteriology

authors： Liang ST,Xu YC,Dennis P,Bremer H

更新日期：2000-06-01 00:00:00

abstract：:From citrate-utilizing (Cit+) Escherichia coli strain C53 of bovine origin, strains C53A and C53B were obtained. Upon mating with recA+ but not with recA mutant recipients of K-12, C53A produced chromosomal recombinants at quite high frequencies, leading to the following conclusions: (i) C53A is an Hfr strain; (ii) th...

journal_title：Journal of bacteriology

authors： Ishiguro N,Oka C,Asagi M,Sato G,Yoshikawa M

更新日期：1980-08-01 00:00:00

abstract：:Carotovoricin Er is a phage-tail-like bacteriocin produced by Erwinia carotovora subsp. carotovora strain Er, a causative agent for soft rot disease in plants. Here we studied binding and killing spectra of carotovoricin Er preparations for various strains of the bacterium (strains 645Ar, EC-2, N786, and P7) and found...

journal_title：Journal of bacteriology

authors： Nguyen HA,Tomita T,Hirota M,Kaneko J,Hayashi T,Kamio Y

更新日期：2001-11-01 00:00:00

abstract：:THREE PREVIOUSLY STUDIED R FACTORS WERE USED: 222/R4, controlling transmissible resistance to sulfonamide, streptomycin, chloromycetin, and tetracycline (SU(r) SM(r) CM(r) TC(r)); 222/R3, a derivative of 222/R4 (now termed 222/R3W) having lost TC(r); and R15, controlling infectious resistance to SU and SM only. Two ty...

journal_title：Journal of bacteriology

authors： Nisioka T,Mitani M,Clowes RC

更新日期：1970-07-01 00:00:00

abstract：:Exposure of Escherichia coli strains deficient in molybdopterin biosynthesis (moa) to the purine base N-6-hydroxylaminopurine (HAP) is mutagenic and toxic. We show that moa mutants exposed to HAP also exhibit elevated mutagenesis, a hyperrecombination phenotype, and increased SOS induction. The E. coli rdgB gene encod...

journal_title：Journal of bacteriology

authors： Burgis NE,Brucker JJ,Cunningham RP

更新日期：2003-05-01 00:00:00

abstract：:Most isolates of Salmonella enterica serovar Typhimurium contain a 90-kb virulence plasmid. This plasmid is reported to be mobilizable but nonconjugative. However, we have determined that the virulence plasmid of strains LT2, 14028, and SR-11 is indeed self-transmissible. The plasmid of strain SL1344 is not. Optimal c...

journal_title：Journal of bacteriology

authors： Ahmer BM,Tran M,Heffron F

更新日期：1999-02-01 00:00:00

abstract：:We have sequenced the entire region of DNA required for the biosynthesis of CS5 pili from enterotoxigenic Escherichia coli O115:H40 downstream of the major subunit gene, designated csfA (for coli surface factor five A). Five more open reading frames (ORFs) (csfB, csfC, csfE, csfF, and csfD) which are transcribed in th...

journal_title：Journal of bacteriology

authors： Duthy TG,Staendner LH,Manning PA,Heuzenroeder MW

更新日期：1999-09-01 00:00:00

abstract：:Youmans, Anne S. (Northwestern University Medical School, Chicago, Ill.), and Guy P. Youmans. Effect of trypsin and ribonuclease on the immunogenic activity of ribosomes and ribonucleic acid isolated from Myobacterium tuberculosis. J. Bacteriol. 91:2146-2154. 1966.-The ribosomal fraction of the attenuated strain, H37R...

journal_title：Journal of bacteriology

authors： Youmans AS,Youmans GP

更新日期：1966-06-01 00:00:00

abstract：:In the chloroplasts of higher plants and algae, the biosynthesis of the chlorophyll precursor delta-aminolevulinic acid (ALA) involves at least three enzymes and a tRNA species. Here we demonstrate that in cell extracts of the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 ALA was formed from glutamate i...

journal_title：Journal of bacteriology

authors： O'Neill GP,Peterson DM,Schön A,Chen MW,Söll D

更新日期：1988-09-01 00:00:00

abstract：:We have isolated mutations that block sporulation after formation of the polar septum in Bacillus subtilis. These mutations were mapped to the two genes of a new locus, spoIIS. Inactivation of the second gene, spoIISB, decreases sporulation efficiency by 4 orders of magnitude. Inactivation of the first gene, spoIISA, ...

journal_title：Journal of bacteriology

authors： Adler E,Barák I,Stragier P

更新日期：2001-06-01 00:00:00

abstract：:Two plasmids containing the penicillinase gene of alkalophilic Bacillus sp. strain 170, pEAP1 and pEAP2, were constructed. Most of the penicillinase produced by Escherichia coli, which carried these plasmids, was found in the culture medium. This excretion is caused by the cloned DNA fragment which contains some compo...

journal_title：Journal of bacteriology

authors： Kudo T,Kato C,Horikoshi K

更新日期：1983-11-01 00:00:00

abstract：:Bacteria use three distinct systems for iron-sulfur (Fe/S) cluster biogenesis: the ISC, SUF, and NIF machineries. The ISC and SUF systems are widely distributed, and many bacteria possess both of them. In Escherichia coli, ISC is the major and constitutive system, whereas SUF is induced under iron starvation and/or ox...

journal_title：Journal of bacteriology

authors： Albrecht AG,Netz DJ,Miethke M,Pierik AJ,Burghaus O,Peuckert F,Lill R,Marahiel MA

更新日期：2010-03-01 00:00:00

abstract：:Acidithiobacillus caldus is an extremely acidophilic, moderately thermophilic, chemolithoautotrophic gammaproteobacterium that derives energy from the oxidation of sulfur and reduced inorganic sulfur compounds. Here we present the draft genome sequence of Acidithiobacillus caldus ATCC 51756 (the type strain of the spe...

journal_title：Journal of bacteriology

authors： Valdes J,Quatrini R,Hallberg K,Dopson M,Valenzuela PD,Holmes DS

更新日期：2009-09-01 00:00:00

abstract：:Twenty-six FliF monomers assemble into the MS ring, a central motor component of the bacterial flagellum that anchors the structure in the inner membrane. Approximately 100 amino acids at the C terminus of FliF are exposed to the cytoplasm and, through the interaction with the FliG switch protein, a component of the f...

journal_title：Journal of bacteriology

authors： Grünenfelder B,Gehrig S,Jenal U

更新日期：2003-03-01 00:00:00

abstract：:Studies were made on the physical and chemical properties of polysaccharides synthesized by cell-free extracts of Streptococcus mutans, Streptococcus sanguis, and Streptococcus sp. and their susceptibilities to dextranases. Among the polysaccharides examined, insoluble glucans were rather resistant to available dextra...

journal_title：Journal of bacteriology

authors： Ebisu S,Kato K,Kotani S,Misaki A

更新日期：1975-12-01 00:00:00

abstract：:The nucleotide sequence of the Clostridium thermocellum F1 xynC gene, which encodes the xylanase XynC, consists of 1,857 bp and encodes a protein of 619 amino acids with a molecular weight of 69,517. XynC contains a typical N-terminal signal peptide of 32 amino acid residues, followed by a 165-amino-acid sequence whic...

journal_title：Journal of bacteriology

authors： Hayashi H,Takagi KI,Fukumura M,Kimura T,Karita S,Sakka K,Ohmiya K

更新日期：1997-07-01 00:00:00