Abstract:
:Exposure of Escherichia coli strains deficient in molybdopterin biosynthesis (moa) to the purine base N-6-hydroxylaminopurine (HAP) is mutagenic and toxic. We show that moa mutants exposed to HAP also exhibit elevated mutagenesis, a hyperrecombination phenotype, and increased SOS induction. The E. coli rdgB gene encodes a protein homologous to a deoxyribonucleotide triphosphate pyrophosphatase from Methanococcus jannaschii that shows a preference for purine base analogs. moa rdgB mutants are extremely sensitive to killing by HAP and exhibit increased mutagenesis, recombination, and SOS induction upon HAP exposure. Disruption of the endonuclease V gene, nfi, rescues the HAP sensitivity displayed by moa and moa rdgB mutants and reduces the level of recombination and SOS induction, but it increases the level of mutagenesis. Our results suggest that endonuclease V incision of DNA containing HAP leads to increased recombination and SOS induction and even cell death. Double-strand break repair mutants display an increase in HAP sensitivity, which can be reversed by an nfi mutation. This suggests that cell killing may result from an increase in double-strand breaks generated when replication forks encounter endonuclease V-nicked DNA. We propose a pathway for the removal of HAP from purine pools, from deoxynucleotide triphosphate pools, and from DNA, and we suggest a general model for excluding purine base analogs from DNA. The system for HAP removal consists of a molybdoenzyme, thought to detoxify HAP, a deoxyribonucleotide triphosphate pyrophosphatase that removes noncanonical deoxyribonucleotide triphosphates from replication precursor pools, and an endonuclease that initiates the removal of HAP from DNA.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Burgis NE,Brucker JJ,Cunningham RPdoi
10.1128/jb.185.10.3101-3110.2003keywords:
subject
Has Abstractpub_date
2003-05-01 00:00:00pages
3101-10issue
10eissn
0021-9193issn
1098-5530journal_volume
185pub_type
杂志文章abstract::The ftsH gene encodes an ATP- and Zn(2+)-dependent metalloprotease which is anchored to the cytoplasmic membrane via two transmembrane segments in such a way that the very short amino- and the long carboxy termini are exposed to the cytoplasm. Deletion of the ftsH gene in Bacillus subtilis results in a pleiotropic phe...
journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
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更新日期:1976-07-01 00:00:00
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
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更新日期:1990-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1992-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.13.4245-4249.1993
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.2.822-824.1978
更新日期:1978-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1983-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.3.1125-1132.1983
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
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更新日期:1985-07-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2003-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1980-06-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2020-03-11 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1961-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2011-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2003-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1978-02-01 00:00:00