Catechol 1,2-dioxygenase from Acinetobacter calcoaceticus: purification and properties.

abstract：:Members of all four families of methanogenic bacteria were analyzed for polyamine concentrations. High-performance liquid chromatography analysis of dansylated cell extracts revealed typical polyamine patterns for each family. Members of Methanobacteriaceae (family I) were characterized by very low polyamine concentra...

journal_title：Journal of bacteriology

authors： Scherer P,Kneifel H

更新日期：1983-06-01 00:00:00

abstract：:Multiple copies of a gene require enhanced investment on the part of the cell and, as such, call for an explanation. The observation that Escherichia coli has four copies of initiator tRNA (tRNAi) genes, encoding a special tRNA (tRNA(fMet)) required to start protein synthesis, is puzzling particularly because the cell...

journal_title：Journal of bacteriology

authors： Samhita L,Nanjundiah V,Varshney U

更新日期：2014-07-01 00:00:00

abstract：:Dehority, B. A. (Ohio Agricultural Experiment Station, Wooster). Degradation and utilization of isolated hemicellulose by pure cultures of cellulolytic rumen bacteria. J. Bacteriol. 89:1515-1520. 1965.-Hemicelluloses isolated from flax, corn hulls, alfalfa, oat hulls, and fescue grass were used as sole energy sources ...

journal_title：Journal of bacteriology

authors： DEHORITY BA

更新日期：1965-06-01 00:00:00

abstract：:A mutant of yeast lacking proteinase C (carboxypeptidase Y) activity has been found by using a histochemical stain to screen mutagenized colonies. This defect segregates 2:2 in meiotic tetrads. Cell extracts lacked the esterolytic, amidase, and proteolytic activities associated with proteinase C. The absence of protei...

journal_title：Journal of bacteriology

authors： Wolf DH,Fink GR

更新日期：1975-09-01 00:00:00

abstract：:The ftsZ gene is thought to be an essential cell division gene in Escherichia coli. We constructed a null allele of ftsZ in a strain carrying additional copies of ftsZ on a plasmid with a temperature-sensitive replication defect. This strain was temperature sensitive for cell division and viability, confirming that ft...

journal_title：Journal of bacteriology

authors： Dai K,Lutkenhaus J

更新日期：1991-06-01 00:00:00

abstract：:The copy number and stability of artificial 2-micron circle-based plasmids have been accurately measured in [Cir+] and [Cir0] strains of Saccharomyces cerevisiae. We conclude that (i) instability and copy number vary greatly from plasmid to plasmid; (ii) instability and copy number are negatively correlated--that is, ...

journal_title：Journal of bacteriology

authors： Futcher AB,Cox BS

更新日期：1984-01-01 00:00:00

abstract：:A mutant of Escherichia coli K-12 was examined which has growth medium-dependent lysyl-transfer ribonucleic acid (tRNA) ligase activity. In minimal medium or 0.5% yeast extract, the activity of the enzyme in the mutant strain was 5 to 10% of wild type. However, when the mutant was grown in a highly enriched medium, su...

journal_title：Journal of bacteriology

authors： Hirshfield IN,Bukald NE

更新日期：1973-01-01 00:00:00

abstract：:We present the nucleotide sequence of the oriT region from plasmid R100. Comparison to other IncF plasmids revealed homology around the proposed nick sites as well as conservation of inverted repeated sequences in the nonhomologous region. Three areas showed strong homology (eight of nine nucleotides) to the consensus...

journal_title：Journal of bacteriology

authors： McIntire SA,Dempsey WB

更新日期：1987-08-01 00:00:00

abstract：:A procedure is presented for the rapid screening of bacterial colonies to detect mutants unable to produce 14CO2 from a labeled precursor. The method is especially useful for mass screening for mutants that cannot be easily detected by their phenotypic characteristics. ...

journal_title：Journal of bacteriology

authors： Tabor H,Tabor CW,Hafner EW

更新日期：1976-10-01 00:00:00

abstract：:Streptococcus bovis possesses two sugar phosphate phosphatases (Pases). Pase I is a soluble enzyme that is inhibited by the membrane fractions from lactose-grown cells and is insensitive to activation by S46D HPr, an analog of HPr(ser-P) of the sugar phosphotransferase system. Pase II is a membrane-associated enzyme t...

journal_title：Journal of bacteriology

authors： Cook GM,Ye JJ,Russell JB,Saier MH Jr

更新日期：1995-12-01 00:00:00

abstract：:The ends of the bacterial transposon gamma delta contain adjacent binding sites for gamma delta transposase and integration host factor (IHF). IHF+ and IHF- strains were used in conjunction with gamma delta transposon ends containing or lacking the site for IHF binding to determine the role that IHF plays in various g...

journal_title：Journal of bacteriology

authors： Wiater LA,Grindley ND

更新日期：1990-09-01 00:00:00

abstract：:The protein surface layers on the cell walls of mosquito-pathogenic and nonpathogenic Bacillus sphaericus strains were studied by structural, biochemical, and serological methods. The surface structure of two representative insect-pathogenic strains had the form of a delicate linear array with a repeat interval of 5 n...

journal_title：Journal of bacteriology

authors： Lewis LO,Yousten AA,Murray RG

更新日期：1987-01-01 00:00:00

abstract：:Pseudomonas oleovorans contains an isomerase which catalyzes the cis-trans conversion of the abundant unsaturated membrane fatty acids 9-cis-hexadecenoic acid (palmitoleic acid) and 11-cis-octadecenoic acid (vaccenic acid). We purified the isomerase from the periplasmic fraction of Pseudomonas oleovorans. The molecula...

journal_title：Journal of bacteriology

authors： Pedrotta V,Witholt B

更新日期：1999-05-01 00:00:00

abstract：:Temperature-resistant pseudorevertants of the temperature-sensitive Escherichia coli mutant KNS19, harboring a mutation in rplX, the gene for ribosomal protein L24, were isolated, cloned, and sequenced. The codon GAC for the amino acid Asp in the temperature-sensitive mutant corresponding to position 84 in the protein...

journal_title：Journal of bacteriology

authors： Nishi K,Müller M,Schnier J

更新日期：1987-10-01 00:00:00

abstract：:A DNA probe from an internal fragment of the exotoxin A structural gene was used to study the effects of selected culture conditions on steady-state levels of exotoxin-specific mRNA in Pseudomonas aeruginosa. Cells grown under conditions of iron deprivation began to synthesize and excrete the exotoxin A polypeptide du...

journal_title：Journal of bacteriology

authors： Lory S

更新日期：1986-12-01 00:00:00

abstract：:In previous work, we cloned a Candida albicans cDNA for the glycolytic enzyme enolase and found a single, abundant enolase transcript on Northern (RNA) blots and a single protein on immunoblots, using antiserum raised against a recombinant enolase fusion protein. Because C. albicans enolase is abundantly produced duri...

journal_title：Journal of bacteriology

authors： Postlethwait P,Sundstrom P

更新日期：1995-04-01 00:00:00

abstract：:The catB and catC genes encode cis,cis-muconate lactonizing enzyme I (EC 5.5.1.1) and muconolactone isomerase (EC 5.3.3.4), respectively. These enzymes are required for the dissimilation of benzoate to beta-ketoadipate by Pseudomonas putida and are under coordinate transcriptional regulation. By deletion analysis and ...

journal_title：Journal of bacteriology

authors： Aldrich TL,Chakrabarty AM

更新日期：1988-03-01 00:00:00

abstract：:In Escherichia coli K-12, two enzymes, encoded by ilvE and tyrB, catalyze the amination of 2-ketoisocaproate (2-KIC) to form leucine. Although leucine-requiring derivatives of an ilvE strain that are unable to grow on 2-KIC were expected to have mutations only in tyrB, mapping studies showed that one such mutation was...

journal_title：Journal of bacteriology

authors： Vartak NB,Liu L,Wang BM,Berg CM

更新日期：1991-06-01 00:00:00

abstract：:Tn916 [carries tet(M)] is a 16.4-kb conjugative transposon that can establish itself in multiple copies in Enterococcus faecalis. To study the interaction of coresident homologous transposons during conjugation, an E. faecalis mutant defective in homologous recombination was utilized for construction of strains harbor...

journal_title：Journal of bacteriology

authors： Flannagan SE,Clewell DB

更新日期：1991-11-01 00:00:00

abstract：:LcrV, an essential piece of the Yop virulon, is encoded by the large lcrGVsycDyopBD operon. In spite of repeated efforts, the role of LcrV in the Yop virulon remains elusive. In an attempt to clarify this, we engineered a complete deletion of lcrV in the pYV plasmid of Yersinia enterocolitica E40 and characterized the...

journal_title：Journal of bacteriology

authors： Sarker MR,Neyt C,Stainier I,Cornelis GR

更新日期：1998-03-01 00:00:00

abstract：:Two mutations concerned with mannan biosynthesis in the yeast Saccharomyces cerevisiae have been mapping. The mnn2 mutation, which affects the addition to the polysaccharide backbone of the first side-chain D-mannose unit in alpha1-leads to2 linkage, was located on chromosome II linked to the centromere and the gall l...

journal_title：Journal of bacteriology

authors： Ballou DL

更新日期：1975-08-01 00:00:00

abstract：:Myxococcus xanthus, a gram-negative bacterium, has a complex life cycle. In response to starvation, most cells in a population participate in the formation of multicellular aggregates (i.e., fruiting bodies) in which cells differentiate into spores. However, some cells do not enter aggregates. In this and the two acco...

journal_title：Journal of bacteriology

authors： O'Connor KA,Zusman DR

更新日期：1991-06-01 00:00:00

abstract：:The expression pattern of the Escherichia coli genome is controlled in part by regulating the utilization of a limited number of RNA polymerases among a total of its approximately 4,600 genes. The distribution pattern of RNA polymerase changes from modulation of two types of protein-protein interactions: the interacti...

journal_title：Journal of bacteriology

authors： Ishihama A,Kori A,Koshio E,Yamada K,Maeda H,Shimada T,Makinoshima H,Iwata A,Fujita N

更新日期：2014-08-01 00:00:00

abstract：:In Escherichia coli, the CpxRA two-component signal transduction system senses and responds to aggregated and misfolded proteins in the bacterial envelope. We show that CpxR-P (the phosphorylated form of the cognate response regulator) activates cpxRA expression in conjunction with RpoS, suggesting an involvement of t...

journal_title：Journal of bacteriology

authors： De Wulf P,Kwon O,Lin EC

更新日期：1999-11-01 00:00:00

abstract：:An in vitro constructed plasmid, pVH15, consisting of the entire genome of the plasmid ColE1, the tryptophan operon of Escherichia coli, and regions of the bacteriophage PHI80pt190, spontaneously gave rise in E. coli to a mini-ColE1 plasmid consisting of approximately one-half of the ColE1 genome and a small segment o...

journal_title：Journal of bacteriology

authors： Hershfield V,Boyer HW,Chow L,Helinski DR

更新日期：1976-04-01 00:00:00

abstract：:Cysteine and methionine availability influences many processes in the cell. In bacteria, transcription of the specific genes involved in the synthesis of these two amino acids is usually regulated by different mechanisms or regulators. Pathways for the synthesis of cysteine and methionine and their interconversion wer...

journal_title：Journal of bacteriology

authors： Sperandio B,Polard P,Ehrlich DS,Renault P,Guédon E

更新日期：2005-06-01 00:00:00

abstract：:Streptococcus thermophilus strain CNRZ 302 is unable to ferment galactose, neither that generated intracellularly by lactose hydrolysis nor the free sugar. Nevertheless, sequence analysis and complementation studies with Escherichia coli demonstrated that strain CNRZ 302 contained structurally intact genes for the Lel...

journal_title：Journal of bacteriology

authors： Vaughan EE,van den Bogaard PT,Catzeddu P,Kuipers OP,de Vos WM

更新日期：2001-02-01 00:00:00

abstract：:Sortases are transamidases that covalently link proteins to the peptidoglycan of gram-positive bacteria. The genome of the pathogenic bacterium Listeria monocytogenes encodes two sortases genes, srtA and srtB. The srtA gene product anchors internalin and some other LPXTG-containing proteins to the listerial surface. H...

journal_title：Journal of bacteriology

authors： Bierne H,Garandeau C,Pucciarelli MG,Sabet C,Newton S,Garcia-del Portillo F,Cossart P,Charbit A

更新日期：2004-04-01 00:00:00

abstract：:The effects of temperature and attractants on chemotaxis in psychrotrophic Pseudomonas fluorescens were examined using the Adler capillary assay technique. Several organic acids, amino acids, and uronic acids were shown to be attractants, whereas glucose and its oxidation products, gluconate and 2-ketogluconate, elici...

journal_title：Journal of bacteriology

authors： Lynch WH

更新日期：1980-07-01 00:00:00

abstract：:The ftsZ gene of Escherichia coli, which lies in a cluster of cell division genes at 2 min on the genetic map, codes for a protein which is thought to play a key role in triggering cell division. Using an ftsZ::lacZ operon fusion, we have studied the transcription of the ftsZ gene under conditions in which cell divisi...

journal_title：Journal of bacteriology

authors： Robin A,Joseleau-Petit D,D'Ari R

更新日期：1990-03-01 00:00:00