Proteinase C (carboxypeptidase Y) mutant of yeast.

Abstract:

:A mutant of yeast lacking proteinase C (carboxypeptidase Y) activity has been found by using a histochemical stain to screen mutagenized colonies. This defect segregates 2:2 in meiotic tetrads. Cell extracts lacked the esterolytic, amidase, and proteolytic activities associated with proteinase C. The absence of proteinase C does not affect mitotic growth and has no obvious effect on the formation of viable ascospores or meiotic segregation. The mutant grows on peptides known to be cleaved by proteinase C in vitro. This finding is consistent with the idea that other enzymes exist in vivo with overlapping substrate specificities.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Wolf DH,Fink GR

doi

10.1128/JB.123.3.1150-1156.1975

subject

Has Abstract

pub_date

1975-09-01 00:00:00

pages

1150-6

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

123

pub_type

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