Complete genome sequences of three Pseudomonas aeruginosa isolates with phenotypes of polymyxin B adaptation and inducible resistance.

abstract：UNLABELLED:Streptococcus pneumoniae is able to integrate exogenous DNA into its genome by natural genetic transformation. Transient accumulation of high levels of the only S. pneumoniae alternative σ factor is insufficient for development of full competence without expression of a second competence-specific protein, Co...

journal_title：Journal of bacteriology

authors： Tovpeko Y,Bai J,Morrison DA

更新日期：2016-08-11 00:00:00

abstract：:The central metabolic model for Geobacter sulfurreducens included a single pathway for the biosynthesis of isoleucine that was analogous to that of Escherichia coli, in which the isoleucine precursor 2-oxobutanoate is generated from threonine. 13C labeling studies performed in G. sulfurreducens indicated that this pat...

journal_title：Journal of bacteriology

authors： Risso C,Van Dien SJ,Orloff A,Lovley DR,Coppi MV

更新日期：2008-04-01 00:00:00

abstract：:We sequenced the minimum REPI replication region and the incompatibility determinants of pColV-K30. The minimum replication region contains an open reading frame which corresponds to a 35-kilodalton (kDa) protein. Sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis with maxicells transformed with a numb...

journal_title：Journal of bacteriology

authors： Perez-Casal JF,Gammie AE,Crosa JH

更新日期：1989-04-01 00:00:00

abstract：:In streptococcal species, the key step of competence development is the transcriptional induction of comX, which encodes the alternative sigma factor sigma(X), which positively regulates genes necessary for DNA transformation. In Streptococcus species belonging to the mitis and mutans groups, induction of comX relies ...

journal_title：Journal of bacteriology

authors： Fontaine L,Boutry C,de Frahan MH,Delplace B,Fremaux C,Horvath P,Boyaval P,Hols P

更新日期：2010-03-01 00:00:00

abstract：:In a search for genes that produce hypersensitivity to cadmium salts in Escherichia coli, random transposon mutagenesis with TnphoA was used. One of the mutant strains obtained was sensitive to Cd2+ and Zn2+. Sequence analysis showed that the TnphoA insertion was located in the dsbA gene coding for a periplasmic prote...

journal_title：Journal of bacteriology

authors： Rensing C,Mitra B,Rosen BP

更新日期：1997-04-01 00:00:00

abstract：:Electron cryotomography was used to analyze the structure of the Lyme disease spirochete, Borrelia burgdorferi. This methodology offers a new means for studying the native architecture of bacteria by eliminating the chemical fixing, dehydration, and staining steps of conventional electron microscopy. Using electron cr...

journal_title：Journal of bacteriology

authors： Charon NW,Goldstein SF,Marko M,Hsieh C,Gebhardt LL,Motaleb MA,Wolgemuth CW,Limberger RJ,Rowe N

更新日期：2009-01-01 00:00:00

abstract：:Temperature-sensitive nalA mutants of Escherichia coli have been used to investigate the structure and functions of deoxyribonucleic acid (DNA) gyrase. Extracts of one such mutant (nalA43) had thermosensitive DNA gyrase subunit A activity but normal gyrase subunit B activity, proving definitively that nalA is the stru...

journal_title：Journal of bacteriology

authors： Kreuzer KN,Cozzarelli NR

更新日期：1979-11-01 00:00:00

abstract：:Avian-pathogenic Escherichia coli (APEC) strains cause extraintestinal diseases in avian species. Here, we present the draft genome of an APEC strain (SCI-07) from Brazil that was isolated from skin lesions (gelatinous edema) on the head and periorbital tissues of a laying hen with swollen head syndrome. ...

journal_title：Journal of bacteriology

authors： Rojas TC,Parizzi LP,Tiba MR,Chen L,Pereira GA,Sangal V,Yang J,Yu J,Dias da Silveira W

更新日期：2012-06-01 00:00:00

abstract：:Most mutations in the yajC-secDF operon identified via genetic screens confer a cold-sensitive growth phenotype. Here we report that two of these mutations confer this cold-sensitive phenotype by inactivating the SecDF-YajC complex in protein translocation. ...

journal_title：Journal of bacteriology

authors： Nouwen N,Driessen AJ

更新日期：2005-10-01 00:00:00

abstract：:rRNA synthesis is the rate-limiting step in ribosome synthesis in Escherichia coli. Its regulation has been described in terms of a negative-feedback control loop in which rRNA promoter activity responds to the amount of translation. The feedback nature of this control system was demonstrated previously by artificiall...

journal_title：Journal of bacteriology

authors： Schneider DA,Gourse RL

更新日期：2003-10-01 00:00:00

abstract：:The ends of the bacterial transposon gamma delta contain adjacent binding sites for gamma delta transposase and integration host factor (IHF). IHF+ and IHF- strains were used in conjunction with gamma delta transposon ends containing or lacking the site for IHF binding to determine the role that IHF plays in various g...

journal_title：Journal of bacteriology

authors： Wiater LA,Grindley ND

更新日期：1990-09-01 00:00:00

abstract：:Plasmids for high-level expression of penicillin-binding protein 6 (PBP6) were constructed, giving rise to overproduction of PBP6 under the control of the lambda pR promoter in either the periplasmic or the cytoplasmic space. In contrast to penicillin-binding protein 5 (PBP5), the presence of high amounts of PBP6 in t...

journal_title：Journal of bacteriology

authors： van der Linden MP,de Haan L,Hoyer MA,Keck W

更新日期：1992-12-01 00:00:00

abstract：:Signal transduction protein P(II) is dephosphorylated in Synechocystis sp. strain PCC 6803 by protein phosphatase PphA. To determine the impact of PphA-mediated P(II) dephosphorylation on physiology, the phenotype of a PphA-deficient mutant was analyzed. Mutants lacking either PphA or P(II) were impaired in efficient ...

journal_title：Journal of bacteriology

authors： Kloft N,Forchhammer K

更新日期：2005-10-01 00:00:00

abstract：:Eighty-seven different enterobacteria and pseudomonas strains were examined for the presence of extrachromosomal deoxyribonucleic acid (DNA). Thirty-four strains contained closed circular DNA by the ethidium bromide CsCl density technique. Extrachromosomal DNA was most frequent in Escherichia and Klebsiella strains. T...

journal_title：Journal of bacteriology

authors： Christiansen C,Christiansen G,Bak AL,Stenderup A

更新日期：1973-04-01 00:00:00

abstract：:The Bacillus anthracis endospore loses resistance properties during germination when its cortex peptidoglycan is degraded by germination-specific lytic enzymes (GSLEs). Although this event normally employs several GSLEs for complete cortex removal, the SleB protein alone can facilitate enough cortex hydrolysis to prod...

journal_title：Journal of bacteriology

authors： Heffron JD,Sherry N,Popham DL

更新日期：2011-01-01 00:00:00

abstract：:The cell wall lipopolysaccharides from 17 species belonging to the genus Xanthomonas were extracted from the cells with hot 45% phenol. After purification, the components of the polysaccharide were obtained by acid hydrolysis of the lipopolysaccharide and were quantitatively assayed. Data obtained show that all prepar...

journal_title：Journal of bacteriology

authors： Volk WA

更新日期：1968-03-01 00:00:00

abstract：:Vibrio cholerae is a motile bacterium responsible for the disease cholera, and motility has been hypothesized to be inversely regulated with virulence. We examined the transcription profiles of V. cholerae strains containing mutations in flagellar regulatory genes (rpoN, flrA, flrC, and fliA) by utilizing whole-genome...

journal_title：Journal of bacteriology

authors： Syed KA,Beyhan S,Correa N,Queen J,Liu J,Peng F,Satchell KJ,Yildiz F,Klose KE

更新日期：2009-11-01 00:00:00

abstract：:The cloned gene coding for Bacillus licheniformis penicillinase (penP) was introduced into Escherichia coli in a heat-inducible lambda Qam vector. After induction, significant amounts of penicillinase were synthesized in the new host. The cellular location of the penicillinase was found to be almost exclusively the ou...

journal_title：Journal of bacteriology

authors： Sarvas MO,Palva IA

更新日期：1983-08-01 00:00:00

abstract：:Glucose-6-phosphate dehydrogenase was partially purified from both glucose-grown and iron-glucose-grown Thiobacillus ferrooxidans. The enzyme possesses a dual nucleotide specificity for either nicotinamide adenine dinucleotide phosphate (NADP) or nicotinamide adenine dinucleotide (NAD) and has a molecular weight of 11...

journal_title：Journal of bacteriology

authors： Tabita R,Lundgren DG

更新日期：1971-10-01 00:00:00

abstract：:Cells of Pseudomonas putida NP, Pseudomonas species (NCIB 9816), and a Nocardia species, after growth on naphthalene as sole source of carbon and energy, contain a nicotinamide adenine dinucleotide (NAD+)-dependent enzyme that oxidizes cis-dihydrodiols of mono- and polycyclic aromatic compounds. Similarly, cells of a ...

journal_title：Journal of bacteriology

authors： Patel TR,Gibson DT

更新日期：1976-12-01 00:00:00

abstract：:The potassium, calcium, magnesium, and zinc ion content of cells in exponential and synchronously growing cultures of Escherichia coli B/r was determined with an X-ray fluorescence spectrometer and an atomic absorption spectrophotometer. Cellular potassium, calcium, and magnesium content increased smoothly during the ...

journal_title：Journal of bacteriology

authors： Kung FC,Raymond J,Glaser DA

更新日期：1976-06-01 00:00:00

abstract：:To define additional components of the export machinery of Escherichia coli, I have isolated extragenic suppressors of a mutant [secA(Ts)] that is temperature sensitive for growth and secretion at 37 degrees C. Suppressors that restored growth at 37 degrees C, but that rendered the cell cold sensitive for growth at 28...

journal_title：Journal of bacteriology

authors： Oliver DB

更新日期：1985-01-01 00:00:00

abstract：:The gram-positive bacterium Bacillus subtilis contains two minimal Tat translocases, TatAdCd and TatAyCy, which are each involved in the secretion of one or more specific protein substrates. We have investigated the subcellular localization of the TatA components by employing C-terminal green fluorescent protein (GFP)...

journal_title：Journal of bacteriology

authors： Ridder AN,de Jong EJ,Jongbloed JD,Kuipers OP

更新日期：2009-07-01 00:00:00

abstract：:Mutational analysis of the carboxy-terminal region of Escherichia coli HlyC was performed by site-directed mutagenesis. Replacement of residue Val-127 or Lys-129 reduced the activity of HlyC to about 30 or 60%, respectively, of that of the wild type, while replacement of Gly-128 reduced the activity to less than 1% of...

journal_title：Journal of bacteriology

authors： Guzmán-Verri C,García F,Arvidson S

更新日期：1997-09-01 00:00:00

abstract：:A Butyrivibrio fibrisolvens H17c glgB gene, was isolated by direct selection for colonies that produced clearing on starch azure plates. The gene was expressed in Escherichia coli from its own promoter. The glgB gene consisted of an open reading frame of 1,920 bp encoding a protein of 639 amino acids (calculated Mr, 7...

journal_title：Journal of bacteriology

authors： Rumbak E,Rawlings DE,Lindsey GG,Woods DR

更新日期：1991-11-01 00:00:00

abstract：:Ralstonia eutropha JMP134(pJP4) degrades 3-chlorobenzoate (3-CB) by using two not completely isofunctional, pJP4-encoded chlorocatechol degradation gene clusters, tfdC(I)D(I)E(I)F(I) and tfdD(II)C(II)E(II)F(II). Introduction of several copies of each gene cluster into R. eutropha JMP222, which lacks pJP4 and thus accu...

journal_title：Journal of bacteriology

authors： Pérez-Pantoja D,Ledger T,Pieper DH,González B

更新日期：2003-03-01 00:00:00

abstract：:Bacillus subtilis contains three Fur homologs: Fur, PerR, and Zur. Despite significant sequence similarities, they respond to different stimuli and regulate different sets of genes. DNA target site comparisons indicate that all three paralogs recognize operators with a core 7-1-7 inverted repeat. The corresponding con...

journal_title：Journal of bacteriology

authors： Fuangthong M,Helmann JD

更新日期：2003-11-01 00:00:00

abstract：:Limited proteolysis of the Escherichia coli cell division inhibitor MinC reveals that its dimerization function resides in a structurally autonomous C-terminal domain. We show that cytoplasmic MinC is poised near the monomer-dimer equilibrium and propose that it only becomes entirely dimeric once recruited to the memb...

journal_title：Journal of bacteriology

authors： Szeto TH,Rowland SL,King GF

更新日期：2001-11-01 00:00:00

abstract：:The gene coding for alveolysin, the thiol-activated toxin produced by Bacillus alvei, has been cloned by means of an oligonucleotide based on the known N-terminal sequence of the secreted protein. The complete nucleotide sequence of the gene has been determined. The deduced amino acid sequence of alveolysin shows that...

journal_title：Journal of bacteriology

authors： Geoffroy C,Mengaud J,Alouf JE,Cossart P

更新日期：1990-12-01 00:00:00

abstract：:Previous studies have shown that narL+ is required for nitrate induction of nitrate reductase synthesis and for nitrate inhibition of fumarate reductase synthesis in Escherichia coli. We cloned narL on a 5.1-kilobase HindIII fragment. Our clone also contained a previously unidentified gene, which we propose to designa...

journal_title：Journal of bacteriology

authors： Stewart V,Parales J Jr

更新日期：1988-04-01 00:00:00