Convenient method for detecting 14CO2 in multiple samples: application to rapid screening for mutants.

abstract：:Yersinia pestis, which causes bubonic and pneumonic plague, forms pigmented red colonies on Congo red (CR) dye agar. The hmsHFRS genes required for CR binding (Crb(+)) are genetically linked to virulence-associated genes encoding a siderophore uptake system. These genes are contained in a 102-kb chromosomal pgm locus ...

journal_title：Journal of bacteriology

authors： Hare JM,McDonough KA

更新日期：1999-08-01 00:00:00

abstract：:The flagellar regulon of Brucella melitensis 16M contains 31 genes clustered in three loci on the small chromosome. These genes encode a polar sheathed flagellum that is transiently expressed during vegetative growth and required for persistent infection in a mouse model. By following the expression of three flagellar...

journal_title：Journal of bacteriology

authors： Léonard S,Ferooz J,Haine V,Danese I,Fretin D,Tibor A,de Walque S,De Bolle X,Letesson JJ

更新日期：2007-01-01 00:00:00

abstract：:Cell division of Escherichia coli K-12 strain PA3092 was inhibited by the addition of adenosine 3',5'-phosphate (cAMP), and the cellular morphology was changed from rods into filaments. Nucleoids in the filaments were regularly distributed and septum formation was perfectly inhibited. This inhibition of cell division ...

journal_title：Journal of bacteriology

authors： Utsumi R,Tanabe H,Nakamoto Y,Kawamukai M,Sakai H,Himeno M,Komano T,Hirota Y

更新日期：1981-09-01 00:00:00

abstract：:The opportunistic pathogen Pseudomonas aeruginosa expresses polar type IV pili (TFP), which are responsible for adhesion to various materials and twitching motility on surfaces. Twitching occurs by alternate extension and retraction of TFP, which arise from assembly and disassembly of pilin subunits at the base of the...

journal_title：Journal of bacteriology

authors： Chiang P,Habash M,Burrows LL

更新日期：2005-02-01 00:00:00

abstract：:The type IV pili of plasmid R64 belonging to the type IVB group are required only for liquid mating. They consist of the major and minor components PilS pilin and PilV adhesin, respectively. PilS pilin is first synthesized as a 22-kDa prepilin from the pilS gene and is then processed to a 19-kDa mature pilin by PilU p...

journal_title：Journal of bacteriology

authors： Shimoda E,Muto T,Horiuchi T,Furuya N,Komano T

更新日期：2008-02-01 00:00:00

abstract：:In the photosynthetic bacterium Rhodobacter capsulatus, the enzyme DNA gyrase has been implicated in the expression of genes for anaerobic metabolic processes such as nitrogen fixation and photosynthesis. To assess the involvement of supercoiling in anaerobic gene expression, we have developed an assay to detect in vi...

journal_title：Journal of bacteriology

authors： Cook DN,Armstrong GA,Hearst JE

更新日期：1989-09-01 00:00:00

abstract：:Subgenomic DNA microarrays were employed to evaluate the expression of the accessory gene regulator (agr locus) as well as multiple virulence-associated genes in Staphylococcus aureus. Gene expression was examined during growth of S. aureus in vitro in standard laboratory medium and rabbit serum and in vivo in subcuta...

journal_title：Journal of bacteriology

authors： Yarwood JM,McCormick JK,Paustian ML,Kapur V,Schlievert PM

更新日期：2002-02-01 00:00:00

abstract：:IcsA is an outer membrane protein in the autotransporter family that is required for Shigella flexneri pathogenesis. Following its secretion through the Sec translocon, IcsA is incorporated into the outer membrane in a process that depends on YaeT, a component of an outer membrane beta-barrel insertion machinery. We i...

journal_title：Journal of bacteriology

authors： Wagner JK,Heindl JE,Gray AN,Jain S,Goldberg MB

更新日期：2009-02-01 00:00:00

abstract：:Glycine betaine is an effective osmoprotectant for Bacillus subtilis. Its import into osmotically stressed cells led to the buildup of large pools, whose size was sensitively determined by the degree of the osmotic stress imposed. The amassing of glycine betaine caused repression of the formation of an osmostress-adap...

journal_title：Journal of bacteriology

authors： Hoffmann T,Wensing A,Brosius M,Steil L,Völker U,Bremer E

更新日期：2013-02-01 00:00:00

abstract：:Seventy-one methionineless and cysteineless auxotrophs of Pseudomonas aeruginosa were placed into nine groups on the basis of their growth on methionine precursors and the cross-feeding response. Transduction experiments with bacteriophage F116 indicated the presence of four linkage groups among the methionineless mut...

journal_title：Journal of bacteriology

authors： Calhoun DH,Feary TW

更新日期：1969-01-01 00:00:00

abstract：:The butyrogenic genes from Clostridium difficile DSM 1296(T) have been cloned and expressed in Escherichia coli. The enzymes acetyl-coenzyme A (CoA) C-acetyltransferase, 3-hydroxybutyryl-CoA dehydrogenase, crotonase, phosphate butyryltransferase, and butyrate kinase and the butyryl-CoA dehydrogenase complex composed o...

journal_title：Journal of bacteriology

authors： Aboulnaga el-H,Pinkenburg O,Schiffels J,El-Refai A,Buckel W,Selmer T

更新日期：2013-08-01 00:00:00

abstract：:CluA is a cell surface-presented protein that causes cell aggregation and is essential for a high-efficiency conjugation process in Lactococcus lactis. We know from previous work that in addition to promoting cell-to-cell contact, CluA is involved in sex factor DNA transfer. To define the CluA domains involved in aggr...

journal_title：Journal of bacteriology

authors： Stentz R,Gasson M,Shearman C

更新日期：2006-03-01 00:00:00

abstract：:The allosteric pattern of control for 3-deoxy-d-arabino-heptulosonate 7-phosphate (DAHP) synthetase has previously been shown to be strongly conserved among the member species of a given genus in bacteria. The implications of this finding as a procedural tool of bacterial phylogeny were pursued by a study of two organ...

journal_title：Journal of bacteriology

authors： Jensen RA,Stenmark SL

更新日期：1970-03-01 00:00:00

abstract：:When Bacillus subtilis is subjected to phosphate starvation, genes of the Pho regulon are either induced or repressed. Among those induced are genes encoding alkaline phosphatases (APases). A set of isogenic mutants, with a beta-galactosidase gene transcriptionally fused to the inactivated target gene, was used to ide...

journal_title：Journal of bacteriology

authors： Prágai Z,Eschevins C,Bron S,Harwood CR

更新日期：2001-04-01 00:00:00

abstract：:Mutants of Bacillis subtilis that are temperature sensitive for RNA synthesis during sporulation were isolated after selection with a 32P suicide agent. Whole-genome sequencing revealed that two of the mutants carried an identical lesion in the rsbU gene, which encodes a phosphatase that indirectly activates SigB, the...

journal_title：Journal of bacteriology

authors： Rothstein DM,Lazinski D,Osburne MS,Sonenshein AL

更新日期：2017-06-27 00:00:00

abstract：:Three polynucleotide phosphorylase mutations, isolated in heavily mutagenized Escherichia coli strains Q7, Q13, and Q27, were characterized after their transfer by P1 transduction to nearly isogenic strains which lack ribonuclease I. Each strain has a different altered form of polynucleotide phosphorylase. One enzyme ...

journal_title：Journal of bacteriology

authors： Reiner AM

更新日期：1969-03-01 00:00:00

abstract：:Isolated sheaths of Leptothrix discophora SP-6 (ATCC 51168) were tested for susceptibility to degradation by a variety of chemical denaturants and lytic enzymes and found to be resistant to many reagents and enzyme treatments. However, disulfide bond-reducing agents such as dithiothreitol (DTT), beta-mercaptoethanol, ...

journal_title：Journal of bacteriology

authors： Emerson D,Ghiorse WC

更新日期：1993-12-01 00:00:00

abstract：:We isolated a new recF mutant of Escherichia coli K-12 by insertion of transposon Tn5 into the recF gene. This recF400::Tn5 allele displayed the same phenotypic characteristics as the classic recF143 mutation. By using Mu d(Ap lac) fusions, the induction of nine SOS genes, including recA, umuC, dinA, dinB, dinD, dinF,...

journal_title：Journal of bacteriology

authors： Thoms B,Wackernagel W

更新日期：1987-04-01 00:00:00

abstract：:Clinical "superbug" isolates of Pseudomonas aeruginosa were previously observed to be resistant to several antibiotics, including polymyxin B, and/or to have a distinct, reproducible adaptive polymyxin resistance phenotype, identified by observing "skipped" wells (appearance of extra turbid wells) during broth microdi...

journal_title：Journal of bacteriology

authors： Boyle B,Fernandez L,Laroche J,Kukavica-Ibrulj I,Mendes CM,Hancock RW,Levesque RC

更新日期：2012-01-01 00:00:00

abstract：:Burkholderia pseudomallei is the causative agent of melioidosis, an often fatal infection of humans and animals. The virulence of this pathogen is thought to depend on a number of secreted proteins, including the MprA metalloprotease. We observed that MprA is produced upon entry into the stationary phase, when the cel...

journal_title：Journal of bacteriology

authors： Valade E,Thibault FM,Gauthier YP,Palencia M,Popoff MY,Vidal DR

更新日期：2004-04-01 00:00:00

abstract：:The umuDC gene products, whose expression is induced by DNA-damaging treatments, have been extensively characterized for their role in SOS mutagenesis. We have recently presented evidence that supports a role for the umuDC gene products in the regulation of growth after DNA damage in exponentially growing cells, analo...

journal_title：Journal of bacteriology

authors： Murli S,Opperman T,Smith BT,Walker GC

更新日期：2000-02-01 00:00:00

abstract：:Lambda transducing phage gamma 16-2 carries the genes envA, ftsZ, ftsA, ddl, and murC and directs the synthesis of six unique proteins in ultraviolet-irradiated cells. Various derivatives of gamma 16-2 carrying smaller segments of the bacterial deoxyribonucleic acid have also been analyzed for their capacity to direct...

journal_title：Journal of bacteriology

authors： Lutkenhaus JF,Wu HC

更新日期：1980-09-01 00:00:00

abstract：:The levels of transfer ribonucleic acids (tRNAs) specific for 14 amino acids were almost identical in dormant spores and in spores germinated from 6 to 75 min. Germinated spore tRNAs specific for all amino acids tested were between 63 and 93% charged, and there was no significant change in this value from 6 to 75 min ...

journal_title：Journal of bacteriology

authors： Setlow P

更新日期：1974-06-01 00:00:00

abstract：:Expression of the Bacillus licheniformis alpha-amylase gene, amyL, was temporally activated and subject to catabolite repression both in its natural host and when cloned on a 3.55-kilobase fragment in Bacillus subtilis. A subclone from which the promoter region of amyL and sequences upstream from the promoter were del...

journal_title：Journal of bacteriology

authors： Laoide BM,Chambliss GH,McConnell DJ

更新日期：1989-05-01 00:00:00

abstract：:Myxococcus xanthus multicellular fruiting body development is initiated by nutrient limitation at high cell density. Five clustered point mutations (sasB5, -14, -15, -16, and -17) can bypass the starvation and high-cell-density requirements for expression of the 4521 developmental reporter gene. These mutants express ...

journal_title：Journal of bacteriology

authors： Xu D,Yang C,Kaplan HB

更新日期：1998-12-01 00:00:00

abstract：:Resistance to the antifolates methasquin and amethopterin has been studied in new strains of Streptococcus faecium var. durans. Two methasquin-resistant strains (SF/MQ, SF/MQ(T)) and an amethopterin-resistant strain (SF/AM) were selected independently from the wild-type S. faecium var. durans (SF/O). SF/MQ(T) is a thy...

journal_title：Journal of bacteriology

authors： Rader JI,Hutchison DJ

更新日期：1972-04-01 00:00:00

abstract：:An alcohol dehydrogenase (ADH) from hyperthermophilic archaeon Thermococcus guaymasensis was purified to homogeneity and was found to be a homotetramer with a subunit size of 40 ± 1 kDa. The gene encoding the enzyme was cloned and sequenced; this gene had 1,095 bp, corresponding to 365 amino acids, and showed high seq...

journal_title：Journal of bacteriology

authors： Ying X,Ma K

更新日期：2011-06-01 00:00:00

abstract：:The buoyant density in rapidly growing Streptococcus faecium 9790 cells varies over the cell cycle, in contrast to the density in Escherichia coli. Buoyant density in S. faecium was measured by using Percoll (Pharmacia Fine Chemicals, Piscataway, N.J.) density gradients. We found that the mean and coefficient of varia...

journal_title：Journal of bacteriology

authors： Glaser D,Higgins M

更新日期：1989-02-01 00:00:00

abstract：:Resting cells of Corynebacterium nephridii reduce nitrate, nitrite, and nitric oxide to nitrous oxide under anaerobic conditions. Nitrous oxide production from nitrite was optimal from pH 7.0 to 7.4. The stoichiometry of nitrous oxide production from nitrite was 99% of the theoretical-two moles of nitrite was used for...

journal_title：Journal of bacteriology

authors： Renner ED,Becker GE

更新日期：1970-03-01 00:00:00

abstract：:The extracytoplasmic factor (ECF) sigma factor sigma(E) is one of the most studied sigma factors of Mycobacterium tuberculosis. It has been shown to be involved in virulence as well as in survival under conditions of high temperature, alkaline pH, and exposure to detergents and oxidative stress. Unlike many ECF sigma ...

journal_title：Journal of bacteriology

authors： Donà V,Rodrigue S,Dainese E,Palù G,Gaudreau L,Manganelli R,Provvedi R

更新日期：2008-09-01 00:00:00