Abstract:
:A DNA probe from an internal fragment of the exotoxin A structural gene was used to study the effects of selected culture conditions on steady-state levels of exotoxin-specific mRNA in Pseudomonas aeruginosa. Cells grown under conditions of iron deprivation began to synthesize and excrete the exotoxin A polypeptide during the late exponential phase of growth and throughout the stationary phase of growth, concomitant with a sharp increase in exotoxin A mRNA pools in P. aeruginosa cells. The addition of iron to the medium resulted in the failure of these cells to synthesize exotoxin A mRNA, despite significantly enhanced growth. The inhibition of the production of exotoxin A and the accumulation of its mRNA by iron was dose dependent, with a half-maximal inhibitory concentration of FeSO4 of 5 to 10 microM. A blockade of the initiation of transcription by rifampin resulted in the decay of exotoxin A mRNA, with a half-life of approximately 8 to 10 min, depending on the media used for growth. The addition of iron to cells actively engaged in exotoxin A synthesis also resulted in a gradual decrease in the amount of this mRNA in bacteria. However, the rate of decline of mRNA induced by iron was relatively slow (half-life, 90 min), with a considerable lag time between the iron addition and the first detectable effect on mRNA. While iron clearly appears to influence the production of exotoxin A at the transcriptional level, the molecular basis of this effect may involve several interacting factors affecting the initiation of transcription and perhaps mRNA turnover.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Lory Sdoi
10.1128/jb.168.3.1451-1456.1986subject
Has Abstractpub_date
1986-12-01 00:00:00pages
1451-6issue
3eissn
0021-9193issn
1098-5530journal_volume
168pub_type
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journal_title:Journal of bacteriology
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doi:10.1128/JB.149.2.768-770.1982
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doi:10.1128/JB.104.1.360-362.1970
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doi:10.1128/jb.178.20.6070-6073.1996
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.18.5861-5863.1994
更新日期:1994-09-01 00:00:00
abstract::Streptococcus suis serotype 2 is a highly invasive, extracellular pathogen in pigs with the capacity to cause severe infections in humans. This study was initiated by the finding that IgM degradation products are released after opsonization of S. suis. The objective of this work was to identify the bacterial factor re...
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pub_type: 杂志文章
doi:10.1128/JB.01875-12
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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doi:10.1128/JB.98.2.403-406.1969
更新日期:1969-05-01 00:00:00
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doi:10.1128/JB.112.1.48-57.1972
更新日期:1972-10-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.140.2.567-573.1979
更新日期:1979-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.9.2541-2551.1993
更新日期:1993-05-01 00:00:00
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更新日期:2008-08-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2016-08-11 00:00:00
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doi:10.1128/JB.126.2.977-984.1976
更新日期:1976-05-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2014-01-01 00:00:00
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更新日期:2003-05-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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更新日期:2007-01-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/jb.174.1.327-330.1992
更新日期:1992-01-01 00:00:00
abstract::This study shows that sequential introduction of drug resistance mutations substantially increased enzyme production in Paenibacillus agaridevorans The triple mutant YT478 (rsmG Gln225→stop codon, rpsL K56R, and rpoB R485H), generated by screening for resistance to streptomycin and rifampin, expressed a 1,100-fold-lar...
journal_title:Journal of bacteriology
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更新日期:2018-08-10 00:00:00
abstract::The flagellar regulon of Brucella melitensis 16M contains 31 genes clustered in three loci on the small chromosome. These genes encode a polar sheathed flagellum that is transiently expressed during vegetative growth and required for persistent infection in a mouse model. By following the expression of three flagellar...
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更新日期:2007-01-01 00:00:00