Abstract:
:The terminal enzyme of heme biosynthesis, ferrochelatase (EC 4.99.1.1), catalyzes the insertion of ferrous iron into protoporphyrin IX to form protoheme. Prior to the present work, [2Fe-2S] clusters have been identified and characterized in animal ferrochelatases but not in plant or prokaryotic ferrochelatases. Herein we present evidence that ferrochelatases from the bacteria Caulobacter crescentus and Mycobacterium tuberculosis possess [2Fe-2S] clusters. The enzyme from C. crescentus is a homodimeric, membrane-associated protein while the enzyme from M. tuberculosis is monomeric and soluble. The clusters of the C. crescentus and M. tuberculosis ferrochelatases are ligated by four cysteines but possess ligand spacings that are unlike those of any previously characterized [2Fe-2S] cluster-containing protein, including the ferrochelatase of the yeast Schizosaccharomyces pombe. Thus, the microbial ferrochelatases represent a new group of [2Fe-2S] cluster-containing proteins.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Dailey TA,Dailey HAdoi
10.1128/jb.184.9.2460-2464.2002keywords:
subject
Has Abstractpub_date
2002-05-01 00:00:00pages
2460-4issue
9eissn
0021-9193issn
1098-5530journal_volume
184pub_type
杂志文章abstract::Proteus mirabilis swarming behavior is characterized by the development of concentric rings of growth that are formed as cyclic events of swarmer cell differentiation, swarming migration, and cellular differentiation are repeated during colony translocation across a surface. This cycle produces the bull's-eye colony o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
abstract::The large virulence plasmid pMYSH6000 of Shigella flexneri contains a determinant that is highly effective in stabilizing otherwise unstable plasmids in Escherichia coli. Expression of two small contiguous genes, mvpA and mvpT (formerly termed STBORF1 and STBORF2), was shown to be sufficient for stability. Mutations i...
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pub_type: 杂志文章
doi:10.1128/jb.182.9.2416-2421.2000
更新日期:2000-05-01 00:00:00
abstract::Formate dehydrogenase has traditionally been assumed to play an essential role in energy generation during growth on C(1) compounds. However, this assumption has not yet been experimentally tested in methylotrophic bacteria. In this study, a whole-genome analysis approach was used to identify three different formate d...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.1.22-28.2004
更新日期:2004-01-01 00:00:00
abstract::A second cluster of genes encoding the E1 alpha, E1 beta, and E2 subunits of branched-chain alpha-keto acid dehydrogenase (BCDH), bkdFGH, has been cloned and characterized from Streptomyces avermitilis, the soil microorganism which produces anthelmintic avermectins. Open reading frame 1 (ORF1) (bkdF, encoding E1 alpha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.12.3504-3511.1995
更新日期:1995-06-01 00:00:00
abstract::Although four of the five Salmonella pathogenicity islands (SPIs) have been characterized in detail for Salmonella enterica serovar Typhimurium, and the fifth has been characterized for Salmonella enterica serovar Dublin, there have been limited studies to examine them in detail in a range of pathogenic serovars of S....
journal_title:Journal of bacteriology
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更新日期:2003-06-01 00:00:00
abstract::During growth of the halophilic archaeon Haloarcula marismortui on D-xylose, a specific D-xylose dehydrogenase was induced. The enzyme was purified to homogeneity. It constitutes a homotetramer of about 175 kDa and catalyzed the oxidation of xylose with both NADP+ and NAD+ as cosubstrates with 10-fold higher affinity ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.18.6198-6207.2004
更新日期:2004-09-01 00:00:00
abstract::The alpha-aminoadipate pathway for the biosynthesis of lysine is present only in fungi and euglena. Until now, this unique metabolic pathway has never been investigated in the opportunistic fungal pathogens Candida albicans, Cryptococcus neoformans, and Aspergillus fumigatus. Five of the eight enzymes (homocitrate syn...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.22.7379-7384.1992
更新日期:1992-11-01 00:00:00
abstract::Willett, Norman P. (University of Pennsylvania School of Veterinary Medicine, Kennett Square, Pa.), and Guy E. Morse. Long-chain fatty acid inhibition of growth of Streptococcus agalactiae in a chemically defined medium. J. Bacteriol. 91:2245-2250. 1966.-A chemically defined medium was developed for Streptococcus agal...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.6.2245-2250.1966
更新日期:1966-06-01 00:00:00
abstract::The 2,647-bp nucleotide sequence of cryptic plasmid pTD1, isolated from the oral spirochete Treponema denticola, was determined. The sequence revealed two open reading frames, A and B, which encode polypeptides of 335 and 235 amino acids, respectively. Open reading frame A shows sequence similarity to genes that encod...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.8.2724-2728.1992
更新日期:1992-04-01 00:00:00
abstract:UNLABELLED:Mycobacterium tuberculosis possesses a thick and highly hydrophobic cell wall principally composed of a mycolyl-arabinogalactan-peptidoglycan complex, which is critical for survival and virulence. DprE1 is a well-characterized component of decaprenyl-phospho-ribose epimerase, which produces decaprenyl-phosph...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00628-15
更新日期:2015-12-01 00:00:00
abstract::During conjugation, a single strand of DNA is cleaved at the origin of transfer (oriT) by the plasmid-encoded relaxase. This strand is then unwound from its complement and transferred in the 5'-to-3' direction, with the 3' end likely extended by rolling-circle replication. The resulting, newly synthesized oriT must th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00818-12
更新日期:2012-10-01 00:00:00
abstract::Pakula, Roman (University of Toronto, Toronto, Ontario, Canada). Factors regulating competence in transformation of streptococci. J. Bacteriol. 90:1320-1324. 1965.-The highly transformable group H Streptococcus strain Challis produced an exocellular competence-provoking enzyme capable of converting to the state of com...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.5.1320-1324.1965
更新日期:1965-11-01 00:00:00
abstract::Cyclic nucleotide-independent protein kinase (EC 2.7.1.37) activity was found in the nuclear cap organelle, within which ribosomes of zoospores of Blastocladiella emersonii are sequestered. Two protein kinase activities were resolved from the high-salt wash fraction of zoospore ribosomes by selective adsorption to DEA...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.3.1467-1471.1983
更新日期:1983-06-01 00:00:00
abstract::In streptomycete anthracycline biosynthetic gene clusters, small open reading frames are located just upstream of minimal polyketide synthase genes. aknX is such a gene found in the aklavinone-aclacinomycin biosynthetic gene cluster of Streptomyces galilaeus. In order to identify its function, the aknX gene was expres...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.22.6115-6122.2002
更新日期:2002-11-01 00:00:00
abstract::The enzyme diversity of the cellulolytic system produced by Clostridium cellulolyticum grown on crystalline cellulose as a sole carbon and energy source was explored by two-dimensional electrophoresis. The cellulolytic system of C. cellulolyticum is composed of at least 30 dockerin-containing proteins (designated cell...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00917-06
更新日期:2007-03-01 00:00:00
abstract::Beppu, Michiko (University of Tokyo, Tokyo, Japan), and Kei Arima. Decreased permeability as the mechanism of arsenite resistance in Pseudomonas pseudomallei. J. Bacteriol. 88:151-157. 1964.-The mechanism of arsenite resistance of Pseudomonas pseudomallei strain 54, isolated from soil, was studied by use of radioactiv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.1.151-157.1964
更新日期:1964-07-01 00:00:00
abstract::Two new mutants of Rhodobacter sphaeroides deficient in sulfolipid accumulation were isolated by directly screening mutagenized cell lines for polar lipid composition by thin-layer chromatography of lipid extracts. A genomic clone which complemented the mutations in these two lines, but not the previously described su...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.20.6479-6487.1992
更新日期:1992-10-01 00:00:00
abstract::Rhodocyclales are representative of versatile bacteria that are able to utilize a wide variety of organic compounds for growth, but only a few strains have been isolated in pure culture thus far. Here we present the genome sequence of Methyloversatilis universalis FAM5(T), the first cultivable methylotrophic member of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05331-11
更新日期:2011-09-01 00:00:00
abstract::Aspartate transcarbamylase is synthesized during exponential growth of Bacillus subtilis and is inactivated when the cells enter the stationary phase. This work is a study of the regulation of aspartate transcarbamylase synthesis during growth and the stationary phase. Using specific immunoprecipitation of aspartate t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.3.943-951.1978
更新日期:1978-09-01 00:00:00
abstract::The Escherichia coli strain cs2-68 is a cold-sensitive (c) mutant that forms a long filamentous cell at 20 degrees C with a large nucleoid mass in its central region. We have recently shown that the pcsA68 mutation causing the cs phenotype is a single-base substitution within the dinD gene, a DNA damage-inducible gene...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.13.3854-3859.1996
更新日期:1996-07-01 00:00:00
abstract::Eklund, Curtis (The University of Texas, Austin) and Orville Wyss. Enzyme associated with bacteriophage infection. J. Bacteriol. 84:1209-1215. 1962.-A capsule-digesting enzyme was formed when azotobacter cells were infected with bacteriophage. The enzyme appeared in the medium when the phages lysed the cells. By disru...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.6.1209-1215.1962
更新日期:1962-12-01 00:00:00
abstract::Bacteriocin 28b from Serratia marcescens binds to Escherichia coli outer membrane proteins OmpA and OmpF and to lipopolysaccharide (LPS) core (J. Enfedaque, S. Ferrer, J. F. Guasch, J. Tomás, and M. Requé, Can. J. Microbiol. 42:19-26, 1996). A cosmid-based genomic library of S. marcescens was introduced into E. coli N...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.19.5741-5747.1996
更新日期:1996-10-01 00:00:00
abstract::DNA uptake by natural competence is a central process underlying the genetic plasticity, biology, and virulence of the human respiratory opportunistic pathogen Streptococcus pneumoniae A study reported in this issue (J. Slager, R. Aprianto, and J.-W. Veening, J. Bacteriol. 201:e00780-18, https://doi.org/10.1128/JB.007...
journal_title:Journal of bacteriology
pub_type: 评论,杂志文章
doi:10.1128/JB.00238-19
更新日期:2019-06-10 00:00:00
abstract::Transposon Tn917 mutagenesis of Bacillus subtilis BD99 followed by selection for protonophore resistance led to the isolation of strain MS119, which contained a single Tn917 insertion in an open reading frame whose deduced amino acid sequence was 56.6% identical to that of the Escherichia coli rho gene product. The in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.3.647-654.1993
更新日期:1993-02-01 00:00:00
abstract::The expression of R-factor-mediated resistance to tetracycline has been compared in Proteus mirabilis and Escherichia coli. Resistance to a range of concentrations of tetracycline was significantly lower in P. mirabilis than in E. coli in both induced and repressed states. Indirect evidence showed that conditions whic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.115.1.235-242.1973
更新日期:1973-07-01 00:00:00
abstract::The presence of plasmid gene cadB did not affect Cd2+ accumulation, whereas plasmid gene cadA reduced Cd2+ accumulation by whole cells but not by membrane vesicles. Membrane vesicle studies indicated that Cd2+ uptake occurred via the Mn2+ transport system which was energized by the membrane electrical potential. Mn2+ ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.150.2.973-976.1982
更新日期:1982-05-01 00:00:00
abstract::Chlamydiae are unusual obligately intracellular bacteria that do not synthesize detectable peptidoglycan. However, they possess genes that appear to encode products with peptidoglycan biosynthetic activity. Bioinformatic analysis predicts that chlamydial MurE possesses UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelic acid (...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01029-09
更新日期:2009-12-01 00:00:00
abstract::The genomic organization of the acidophilic chemolithoautotrophic bacterium Thiobacillus ferrooxidans ATCC 21834 has been studied by pulsed-field gel electrophoresis (PFGE). Analysis of its intact DNA, as well as the restriction patterns obtained with several endonucleases, allowed the characterization of one circular...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.6.1946-1950.1997
更新日期:1997-03-01 00:00:00
abstract::Bap (biofilm-associated protein) is a 254-kDa staphylococcal surface protein implicated in formation of biofilms by staphylococci isolated from chronic mastitis infections. The presence of potential EF-hand motifs in the amino acid sequence of Bap prompted us to investigate the effect of calcium on the multicellular b...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.22.7490-7498.2004
更新日期:2004-11-01 00:00:00
abstract::Phytoplasmas are plant-pathogenic bacteria that cause numerous diseases. This study shows a strong positive selection on the phytoplasma antigenic membrane protein (Amp). The ratio of nonsynonymous to synonymous substitutions was >1 with all the methods we tested. The clear positive selections imply an important biolo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.9.3424-3428.2006
更新日期:2006-05-01 00:00:00