Expression, purification, and characterization of AknX anthrone oxygenase, which is involved in aklavinone biosynthesis in Streptomyces galilaeus.

abstract：:By transformation of dnaA null mutant host cells that are suppressed either by an rnh mutation or by chromosomal integration of a mini-R1 plasmid, it was shown that replication of miniplasmids composed of the NR1 minimal replicon had no absolute dependence upon DnaA protein. In addition, the suppression of the dnaA nu...

journal_title：Journal of bacteriology

authors： Tang XB,Womble DD,Rownd RH

更新日期：1989-10-01 00:00:00

abstract：:Transposon Tn917 mutagenesis of Bacillus subtilis BD99 followed by selection for protonophore resistance led to the isolation of strain MS119, which contained a single Tn917 insertion in an open reading frame whose deduced amino acid sequence was 56.6% identical to that of the Escherichia coli rho gene product. The in...

journal_title：Journal of bacteriology

authors： Quirk PG,Dunkley EA Jr,Lee P,Krulwich TA

更新日期：1993-02-01 00:00:00

abstract：:The uropathogenic Escherichia coli strain 536 carries at least five genetic elements on its chromosome that meet all criteria characteristic of pathogenicity islands (PAIs). One main feature of these distinct DNA regions is their instability. We applied the so-called island-probing approach and individually labeled al...

journal_title：Journal of bacteriology

authors： Middendorf B,Hochhut B,Leipold K,Dobrindt U,Blum-Oehler G,Hacker J

更新日期：2004-05-01 00:00:00

abstract：:The chromosomal DNA replication origins (oriC) from two members of the family Enterobacteriaceae, Enterobacter aerogenes and Klebsiella pneumoniae, have been isolated as functional replication origins in Escherichia coli. The origins in the SalI restriction fragments of 17.5 and 10.2 kilobase pairs, cloned from E. aer...

journal_title：Journal of bacteriology

authors： Harding NE,Cleary JM,Smith DW,Michon JJ,Brusilow WS,Zyskind JW

更新日期：1982-12-01 00:00:00

abstract：:A preparation of pure protoplasts of Geotrichum candidum became osmotically stable and colonies developed when the protoplasts were embedded in stabilizing thin-layer-agar and incubated with stabilizing basal medium. When growing protoplasts were exposed to distilled water and then reincubated with basal medium, the p...

journal_title：Journal of bacteriology

authors： Fukui K,Sagara Y,Yoshida N,Matsuoka T

更新日期：1969-04-01 00:00:00

abstract：:Escherichia coli MutY and MutS increase replication fidelity by removing adenines that were misincorporated opposite 7,8-dihydro-8-oxo-deoxyguanines (8-oxoG), G, or C. MutY DNA glycosylase removes adenines from these mismatches through a short-patch base excision repair pathway and thus prevents G:C-to-T:A and A:T-to-...

journal_title：Journal of bacteriology

authors： Bai H,Lu AL

更新日期：2007-02-01 00:00:00

abstract：:The Bacillus subtilis ferric uptake regulator (Fur) protein mediates the iron-dependent repression of at least 20 operons encoding approximately 40 genes. We investigated the physiological roles of Fur-regulated genes by the construction of null mutations in 14 transcription units known or predicted to function in sid...

journal_title：Journal of bacteriology

authors： Ollinger J,Song KB,Antelmann H,Hecker M,Helmann JD

更新日期：2006-05-01 00:00:00

abstract：:Bacillus megaterium mutants JV-9 and JV-10 are temperature sensitive for initiation of spore germination. At 46 C, they did not lose heat resistance, dipicolinic acid, or absorbance, indicating that the temperature-sensitive blocks are very early in the sequence of initiation reactions. Strain JV-9 was temperature sen...

journal_title：Journal of bacteriology

authors： Vary JC

更新日期：1975-01-01 00:00:00

abstract：:Evolutionary trajectories and mutational landscapes of drug-resistant bacteria are influenced by cell-intrinsic and extrinsic factors. In this study, I demonstrated that loss of the Lon protease altered susceptibility of Escherichia coli to trimethoprim and that these effects were strongly contingent on the drug conce...

journal_title：Journal of bacteriology

authors： Matange N

更新日期：2020-01-15 00:00:00

abstract：:In Escherichia coli, cell division is mediated by the concerted action of about 12 proteins that assemble at the division site to presumably form a complex called the divisome. Among these essential division proteins, the multimodular class B penicillin-binding protein 3 (PBP3), which is specifically involved in septa...

journal_title：Journal of bacteriology

authors： Piette A,Fraipont C,Den Blaauwen T,Aarsman ME,Pastoret S,Nguyen-Distèche M

更新日期：2004-09-01 00:00:00

abstract：:Escherichia coli mutants with improved organic solvent tolerance levels showed high levels of outer membrane protein TolC and inner membrane protein AcrA. The TolC level was regulated positively by MarA, Rob, or SoxS. A possible mar-rob-sox box sequence was found upstream of the tolC gene. These findings suggest that ...

journal_title：Journal of bacteriology

authors： Aono R,Tsukagoshi N,Yamamoto M

更新日期：1998-02-01 00:00:00

abstract：:Flac mutants have been isolated in Escherichia coli K-12 which carry dominant mutations resulting in insensitivity to transfer inhibition by the Fin(+) I-like plasmid R62. These mutants were still sensitive to transfer inhibition by the fin(+) F-like plasmid R100 and, conversely, FlactraO(-) and traP(-) mutants, which...

journal_title：Journal of bacteriology

authors： Willetts NS,Paranchych W

更新日期：1974-10-01 00:00:00

abstract：:Martinez, R. J. (University of California, Los Angeles), and E. Z. Gordee. Formation of bacterial flagella. I. Demonstration of a functional flagellin pool in Spirillum serpens and Bacillus subtilis. J. Bacteriol. 91:870-875. 1966-Exponentially growing cultures of Spirillum serpens and Bacillus subtilis regained motil...

journal_title：Journal of bacteriology

authors： Martinez RJ,Gordee EZ

更新日期：1966-02-01 00:00:00

abstract：:We have constructed a promoter-probe expression vector for Bacillus subtilis. This plasmid, pCED6, can be used to fuse various DNA sequences to the structural gene of Escherichia coli beta-galactosidase, permitting analysis of the promoter activity of such sequences. pCED6 replicates and confers drug resistances in bo...

journal_title：Journal of bacteriology

authors： Donnelly CE,Sonenshein AL

更新日期：1984-03-01 00:00:00

abstract：:Accumulation of l-alpha-glycerophosphate, in cells of Bacillus subtilis mutants lacking the nicotinamide adenine dinucleotide-independent glycerophosphate dehydrogenase activity, suppresses both growth and sporulation. After growth has stopped, the cells slowly develop one and later more asymmetric septa that are thic...

journal_title：Journal of bacteriology

authors： O YK,Freese EB,Freese E

更新日期：1973-02-01 00:00:00

abstract：:The conjugative plasmid pTR2030 has been used extensively to confer phage resistance in commercial Lactococcus starter cultures. The plasmid harbors a 16-kb region, flanked by insertion sequence (IS) elements, that encodes the restriction/modification system LlaI and carries an abortive infection gene, abiA. The AbiA ...

journal_title：Journal of bacteriology

authors： Durmaz E,Klaenhammer TR

更新日期：2007-02-01 00:00:00

abstract：:Escherichia coli K antigens (capsular polysaccharides) are divided into two broad classes, designated groups I and II, on the basis of a number of chemical, physical, and genetic criteria. Group I K antigens can be further subdivided on the basis of the absence (group IA) or presence (group IB) of amino sugars in the ...

journal_title：Journal of bacteriology

authors： MacLachlan PR,Keenleyside WJ,Dodgson C,Whitfield C

更新日期：1993-12-01 00:00:00

abstract：:Small nuclear ribonucleoprotein (snRNP) particles play an important role in the processing of pre-mRNA. snRNPs have been identified immunologically in a variety of cells, but none have ever been observed in prokaryotic systems. This report provides the first evidence for the presence of snRNP-like components in two ty...

journal_title：Journal of bacteriology

authors： Kovacs SA,O'Neil J,Watcharapijarn J,Moe-Kirvan C,Vijay S,Silva V

更新日期：1993-04-01 00:00:00

abstract：:Although amino acid transport has been extensively studied in bacteria during the past decade, little is known concerning the transport of those amino acids that are biosynthetic intermediates or have multiple fates within the cell. We have studied homoserine and threonine as examples of this phenomenon. Homoserine is...

journal_title：Journal of bacteriology

authors： Templeton BA,Savageau MA

更新日期：1974-03-01 00:00:00

abstract：:A cloned 8.3-kilobase-pair DNA fragment carrying all the genes (iucABCD iutA) of the aerobactin iron transport system of plasmid pColV-K30 was subjected to in vitro mutagenesis to afford mutant genes iucA, iucC, and iucA iucC. Complementation analyses and identification of aerobactin precursors accumulated by Escheric...

journal_title：Journal of bacteriology

authors： de Lorenzo V,Neilands JB

更新日期：1986-07-01 00:00:00

abstract：:The rnt gene encoding RNase T was cloned on a 13-kilobase BamHI fragment. Restriction analysis of the fragment and comparison of it with the Escherichia coli restriction map localized rnt to kilobase coordinates 1733 to 1746, corresponding to about 36 min on the genetic map. The map location was confirmed by cotransdu...

journal_title：Journal of bacteriology

authors： Case LM,Chen XN,Deutscher MP

更新日期：1989-10-01 00:00:00

abstract：:Recombinant Escherichia coli strains harboring heterologous polyhydroxyalkanoate (PHA) biosynthesis genes were shown to accumulate unusually large amounts of PHA. In the present study, integrated cellular responses of metabolically engineered E. coli to the accumulation of poly(3-hydroxybutyrate) (PHB) in the early st...

journal_title：Journal of bacteriology

authors： Han MJ,Yoon SS,Lee SY

更新日期：2001-01-01 00:00:00

abstract：:A positive selection method for isolation of nitrogenase-derepressed mutant strains of a filamentous cyanobacterium, Anabaena variabilis, is described. Mutant strains that are resistant to a glutamate analog, L-methionine-D,L-sulfoximine, were screened for their ability to produce and excrete NH4+ into medium. Mutant ...

journal_title：Journal of bacteriology

authors： Spiller H,Latorre C,Hassan ME,Shanmugam KT

更新日期：1986-02-01 00:00:00

abstract：:On the basis of information available in the literature, gluconate dissimilation in Escherichia coli is thought to occur via the hexose monophosphate pathway. Evidence is presented in this study that gluconate is catabolized in this organism via an inducible Entner-Doudoroff pathway. This evidence is based on chromato...

journal_title：Journal of bacteriology

authors： Eisenberg RC,Dobrogosz WJ

更新日期：1967-03-01 00:00:00

abstract：:Neisseria gonorrhoeae releases peptidoglycan fragments during growth. The majority of the fragments released are peptidoglycan monomers, molecules known to increase pathogenesis through the induction of proinflammatory cytokines and responsible for the killing of ciliated epithelial cells. In other gram-negative bacte...

journal_title：Journal of bacteriology

authors： Garcia DL,Dillard JP

更新日期：2008-06-01 00:00:00

abstract：:The SecYEG heterotrimeric membrane protein complex functions as a channel for protein translocation across the Escherichia coli cytoplasmic membrane. SecY is the central subunit of the SecYEG complex and contains 10 transmembrane segments (TM1 to TM10). Previous mutation studies suggested that TM3 and TM4 are particul...

journal_title：Journal of bacteriology

authors： Mori H,Shimokawa N,Satoh Y,Ito K

更新日期：2004-06-01 00:00:00

abstract：:The kil-kor regulon of the self-transmissible, broad-host-range plasmid RK2 is a unique network with eight coregulated operons. Among the genes encoded by the kil-kor regulon are trfA, which encodes the replication initiator, and several kil loci (kilA, kilB, kilC, and kilE), each of which is lethal to the host cell i...

journal_title：Journal of bacteriology

authors： Thomson VJ,Jovanovic OS,Pohlman RF,Chang CH,Figurski DH

更新日期：1993-04-01 00:00:00

abstract：:Hasenclever, H. F. (U. S. Public Health Service, Bethesda, Md.), William O. Mitchell, and Joseph Loewe. Antigenic studies of Candida. II. Antigenic relation of Candida albicans group A and group B to Candida stellatoidea and Candida tropicalis. J. Bacteriol. 82:574-577. 1961.-Previous work in our laboratory has shown ...

journal_title：Journal of bacteriology

authors： HASENCLEVER HF,MITCHELL WO,LOEWE J

更新日期：1961-10-01 00:00:00

abstract：:A novel phenotype is described for Escherichia coli K-12 carrying the prlA4 allele determining a membrane component of the protein export mechanism. It is manifest as transformation deficiency for plasmids containing the cloned group C streptococcal streptokinase gene, skc. Streptokinase plasmid mutations relieving th...

journal_title：Journal of bacteriology

authors： Müller J,Reinert H,Malke H

更新日期：1989-04-01 00:00:00

abstract：:During spore formation, Bacillus subtilis divides asymmetrically, resulting in two cells with different fates. Immediately after division, the transcription factor σ(F) becomes active in the smaller prespore, followed by activation of σ(E) in the larger mother cell. We recently showed that a delay in σ(E) activation r...

journal_title：Journal of bacteriology

authors： Xenopoulos P,Piggot PJ

更新日期：2011-06-01 00:00:00