Abstract:
:Although amino acid transport has been extensively studied in bacteria during the past decade, little is known concerning the transport of those amino acids that are biosynthetic intermediates or have multiple fates within the cell. We have studied homoserine and threonine as examples of this phenomenon. Homoserine is transported by a single system which it shares with alanine, cysteine, isoleucine, leucine, phenylalanine, threonine, tyrosine, and valine. The evidence for this being the sole system for homoserine transport is (i) a linear double-reciprocal plot showing a homoserine K(m) of 9.6 x 10(-6) M, (ii) simultaneous reduction by 85% of homoserine and branched-chain amino acid uptake in a mutant selected for its inability to transport homoserine, and (iii) simultaneous reduction by 94% of the uptake of homoserine and the branched-chain amino acids by cells grown in millimolar leucine. Threonine, in addition to sharing the above system with homoserine, is transported by a second system shared with serine. The evidence for this second system consists of (i) incomplete inhibition of threonine uptake by any single amino acid, (ii) only 70% loss of threonine uptake in the mutant unable to transport homoserine, and (iii) only 40% reduction of threonine uptake when cells are grown in millimolar leucine. In this last case, the remaining threonine uptake can only be inhibited by serine and the inhibition is complete.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Templeton BA,Savageau MAdoi
10.1128/JB.117.3.1002-1009.1974subject
Has Abstractpub_date
1974-03-01 00:00:00pages
1002-9issue
3eissn
0021-9193issn
1098-5530journal_volume
117pub_type
杂志文章abstract::Bacteroides fragilis V479-1 (also designated strain 92) has previously been shown to contain a conjugative plasmid, pBF4, that specifies resistance to clindamycin (Cc). A report of inducible tetracycline (Tc) resistance in this strain suggested that this phenotype was also plasmid associated (G. Privitera et al., Natu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.1.281-287.1982
更新日期:1982-07-01 00:00:00
abstract::Cells of the cyanobacterium Anabaena variabilis starved for phosphate for 3 days took up phosphate at about 100 times the rate of unstarved cells. Kinetic data suggested that a new transport system had been induced by starvation for phosphate. The inducible phosphate transport system was quickly repressed by addition ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.3.1143-1147.1988
更新日期:1988-03-01 00:00:00
abstract::Plasmids pRAS3.1 and pRAS3.2 are natural variants of the IncQ-2 plasmid family, that except for two differences, have identical plasmid backbones. Plasmid pRAS3.1 has four 22-bp iterons in its oriV region, while pRAS3.2 has only three 6-bp repeats and pRAS3.1 has five 6-bp repeats in the promoter region of the mobB-mo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00176-10
更新日期:2010-12-01 00:00:00
abstract::Biofilm formation in Staphylococcus epidermidis is dependent upon the ica operon-encoded polysaccharide intercellular adhesin, which is subject to phase-variable and environmental regulation. The icaR gene, located adjacent to the ica operon, appears to be a member of the tetR family of transcriptional regulators. In ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.16.4400-4408.2002
更新日期:2002-08-01 00:00:00
abstract::Kondo, Eiko (Gunma University, Maebashi, Japan), and Susumu Mitsuhashi. Drug resistance of enteric bacteria. IV. Active transducing phage P1 CM produced by the combination of R factor with phage P1. J. Bacteriol. 88:1266-1276. 1964.-During an investigation of the transduction of R factors with phage P1, a phage lysate...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.5.1266-1276.1964
更新日期:1964-11-01 00:00:00
abstract::The obligate psychrophilic yeasts Torulopsis psychrophila, T. austromarina, Leucosporidium frigidum, L. gelidum, and L. nivalis were obligate aerobes and were unable to grow anaerobically. In contrast, the obligate thermophilic yeasts T. bovina, T. pintolopesii, Candida slooffii, and Saccharomyces telluris were facult...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.2.815-817.1978
更新日期:1978-11-01 00:00:00
abstract::As part of our effort to uncover the molecular basis for the phenotypic variation among clinical Mycobacterium tuberculosis isolates, we have previously reported that isolates belonging to the W/Beijing lineage constitutively overexpress the DosR-regulated transcriptional program. While generating dosR knockouts in tw...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00536-10
更新日期:2010-09-01 00:00:00
abstract::A pattern of allosteric control for aromatic biosynthesis in cyanobacteria relies upon early-pathway regulation as the major control point for the entire branched pathway. In Synechococcus sp. strain PCC6301 (Anacystis nidulans), two enzymes which form precursors for L-phenylalanine biosynthesis are subject to control...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.1.423-428.1983
更新日期:1983-01-01 00:00:00
abstract::The DNA of bacteriophage Mu, extracted from induced lysates, is partially resistant to digestion by the endonuclease BalI. This modification of DNA is controlled by the Mu modification function (mom), which acts in conjunction with the dam (DNA-adenine methylation) function of Escherichia coli. Since the BalI recognit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.1.423-428.1978
更新日期:1978-10-01 00:00:00
abstract::Forty-five strains, representing 18 species of the genus Bacillus, were surveyed for production of hemolysin against rabbit erythrocytes. Broth cultures of B. cereus, B. alvei, and B. laterosporus contained lysins that closely resembled streptolysin O. B. subtilis and a single strain of B. cereus may produce lysins ha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.5.1541-1543.1967
更新日期:1967-05-01 00:00:00
abstract::Pep 5 and nisin are cationic peptide antibiotics which in addition to their membrane-disruptive action induce autolysis in staphylococci. To investigate the mechanism of lysis induction, the influence of the peptides on the activity of the N-acetylmuramoyl-L-alanine amidase of Staphylococcus simulans 22 was studied. I...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.12.5452-5458.1987
更新日期:1987-12-01 00:00:00
abstract::Components of membranes isolated from Spiroplasma citri and corn stunt spiroplasma grown at 28 degrees C were analyzed. On a protein basis, lipid phosphorus was lower and cholesterol was higher in S. citri. Only minor differences between the two species were found in fatty acid composition, reduced nicotinamide adenin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.2.1056-1058.1979
更新日期:1979-02-01 00:00:00
abstract:UNLABELLED:Deacetylation of 7-aminocephalosporanic acid (7-ACA) at position C-3 provides valuable starting material for producing semisynthetic β-lactam antibiotics. However, few enzymes have been characterized in this process before now. Comparative analysis of the genome of the thermophilic bacterium Alicyclobacillus...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00471-15
更新日期:2015-11-02 00:00:00
abstract::Myxococcus xanthus dsp and dif mutants have similar phenotypes in that they are deficient in social motility and fruiting body development. We compared the two loci by genetic mapping, complementation with a cosmid clone, DNA sequencing, and gene disruption and found that 16 of the 18 dsp alleles map to the dif genes....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.5.1462-1465.2002
更新日期:2002-03-01 00:00:00
abstract::The anaerobic spore former Clostridioides difficile causes significant diarrheal disease in humans and other mammals. Infection begins with the ingestion of dormant spores, which subsequently germinate within the host gastrointestinal tract. There, the vegetative cells proliferate and secrete two exotoxins, TcdA and T...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00586-19
更新日期:2020-01-02 00:00:00
abstract::Parnas, Józef (Academy of Medicine, Lublin, Poland). Differentiation of brucellae by the aid of phages. J. Bacteriol. 82:319-325. 1961.-There appears to be no absolute specificity of phages for the three species of the genus Brucella. The phage test is only supplementary to biochemical and serological methods for spec...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.3.319-325.1961
更新日期:1961-09-01 00:00:00
abstract::The O-repeating unit of the Escherichia coli O7-specific lipopolysaccharide is made of galactose, mannose, rhamnose, 4-acetamido-4,6-dideoxyglucose, and N-acetyglucosamine. We have recently characterized the genes involved in the biosynthesis of the sugar precursor GDP-mannose occurring in the E. coli O7:K1 strain VW1...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.19.5539-5546.1995
更新日期:1995-10-01 00:00:00
abstract:UNLABELLED:CbrA is an atypical sensor kinase found in Pseudomonas. The autokinase domain is connected to a putative transporter of the sodium/solute symporter family (SSSF). CbrA functions together with its cognate response regulator, CbrB, and plays an important role in nutrient acquisition, including regulation of hu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00361-15
更新日期:2015-09-01 00:00:00
abstract::Bacteriophage GA-1 infects Bacillus sp. strain G1R and has a linear double-stranded DNA genome with a terminal protein covalently linked to its 5' ends. GA-1 protein p6 is very abundant in infected cells and binds DNA with no sequence specificity. We show here that it binds in vivo to the whole viral genome, as detect...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01047-07
更新日期:2007-11-01 00:00:00
abstract::The Vibrio fischeri luminescence (lux) genes are regulated by the 250-amino-acid-residue LuxR protein and a V. fischeri metabolite termed autoinducer. The V. fischeri lux regulon consists of two divergently transcribed units. Autoinducer and LuxR activate transcription of the luxICDABE operon and autoregulate the luxR...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.12.4064-4069.1992
更新日期:1992-06-01 00:00:00
abstract::As a step towards studying representative members of the two-component family of signal transduction proteins, we have cloned genes encoding a histidine protein kinase and a response regulator from the hyperthermophilic bacterium Thermotoga maritima. The genes have been designated HpkA and drrA, respectively. The dedu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.19.5579-5585.1996
更新日期:1996-10-01 00:00:00
abstract::Microarray studies of the Escherichia coli response to nitric oxide and nitrosative stress have suggested that additional transcriptional regulators of this response remain to be characterized. We identify here the product of the yjeB gene as a negative regulator of the transcription of the ytfE, hmpA and ygbA genes, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.3.874-881.2006
更新日期:2006-02-01 00:00:00
abstract::Analysis of rRNA genes in Vibrio fischeri indicates the presence of eight rRNA gene sets in this organism. It was found that the genes for 5S rRNA, 16S rRNA, and 23S rRNA are organized in operons in the following order: 5' end 16S rRNA 23S RNA 5S rRNA 3' end. Although the operons are homologous, they are not identical...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.5.2318-2321.1987
更新日期:1987-05-01 00:00:00
abstract::We previously showed that adsorption of bacteriophages T4 and T5 to their respective outer membrane receptors induced a partial depolarization of the cytoplasmic membrane. As these membrane potential changes were independent of phage properties, we proposed that phage adsorption triggered the emission of a signal whic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.3.789-794.1984
更新日期:1984-03-01 00:00:00
abstract::Previously we have shown that flagella and the O-specific polysaccharide of lipopolysaccharide play a role in colonization of the potato root by plant growth-promoting Pseudomonas strains WCS374 and WCS358. In this paper, we describe a novel cell surface-exposed structure in Pseudomonas putida WCS358 examined with a s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.7.1955-1961.1996
更新日期:1996-04-01 00:00:00
abstract::Proline-requiring mutants of Saccharomyces cerevisiae were isolated. Each mutation is recessive and is inherited as expected for a single nuclear gene. Three complementation groups cold be defined which are believed to correspond to mutations in the three genes (pro1, pro2, and pro3) coding for the three enzymes of th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.3.816-822.1979
更新日期:1979-06-01 00:00:00
abstract::Mutants lacking an enzyme of the oxidative branch of the hexose monophosphate shunt, 6-phosphogluconolactonase (pgl), have been selected as a new class of glucose-negative derivatives of a phosphoglucose isomerase (pgi) mutant. Glucose negativity is not as complete as in mutants lacking phosphoglucose isomerase and gl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.3.1296-1301.1969
更新日期:1969-12-01 00:00:00
abstract::Mutants of Thiosphaera pantotropha defective in chemolithoautotrophic growth were obtained by transpositional mutagenesis with Tn5 coding for kanamycin resistance. The suicide vehicle for introducing Tn5 to T. pantotropha was pSUP5011 harbored by Escherichia coli. Kanamycin-resistant isolates were screened for the ina...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.2.446-452.1986
更新日期:1986-05-01 00:00:00
abstract::The diphtheria toxin repressor (DtxR) uses Fe(2+) as a corepressor and inhibits transcription from iron-regulated promoters (IRPs) in Corynebacterium diphtheriae. A new IRP, designated IRP6, was cloned from C. diphtheriae by a SELEX-like procedure. DtxR bound to IRP6 in vitro only in the presence of appropriate divale...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.17.4846-4856.2002
更新日期:2002-09-01 00:00:00
abstract::Superoxide dismutase (SOD) and catalase (CAT) levels were determined for several aerobically grown halophilic vibrios and compared with those found in aerobically grown Escherichia coli K-12. The SOD levels ranged from 25 to 103.6 U/mg of protein for the vibrios compared with 44.6 U/mg of protein for E. coli. The CAT ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.134.2.375-380.1978
更新日期:1978-05-01 00:00:00