Evolutionary competitiveness of two natural variants of the IncQ-like plasmids, pRAS3.1 and pRAS3.2.

abstract：:Succinate dehydrogenase (EC 1.3.99.1) of Micrococcus luteus was selectively precipitated from Triton X-100-solubilized membranes by using specific antiserum. The precipitated enzyme contained equimolar amounts of four polypeptides with apparent molecular weights of 72,000, 30,000, 17,000, and 15,000. The 72,000 polype...

journal_title：Journal of bacteriology

authors： Crowe BA,Owen P

更新日期：1983-03-01 00:00:00

abstract：:The morphological and physiological characteristics of 20 isolates of psychrophilic Bacillus were compared with 29 strains representing nine species of mesophilic Bacillus and 2 strains of Sporosarcina ureae to determine the taxonomic position of the psychrophiles. The psychrophiles formed four distinct groups which w...

journal_title：Journal of bacteriology

authors： Larkin JM,Stokes JL

更新日期：1967-10-01 00:00:00

abstract：:A large number of carbon source utilization pathways are repressed in Bacillus subtilis by the global regulator CcpA, which also acts as an activator of carbon excretion pathways during growth in media containing glucose. In this study, CcpA mutants defective in transcriptional activation of the alsSD operon, which is...

journal_title：Journal of bacteriology

authors： Turinsky AJ,Moir-Blais TR,Grundy FJ,Henkin TM

更新日期：2000-10-01 00:00:00

abstract：:The glpD (MSMEG_6761) gene encoding glycerol-3-phosphate dehydrogenase was shown to be crucial for M. smegmatis to utilize glycerol as the sole carbon source. The glpD gene likely forms the glpFKD operon together with glpF and glpK, encoding a glycerol facilitator and glycerol kinase, respectively. The gylR (MSMEG_675...

journal_title：Journal of bacteriology

authors： Bong HJ,Ko EM,Song SY,Ko IJ,Oh JI

更新日期：2019-11-20 00:00:00

abstract：:The 2,760-base-pair (bp) PstI-EcoRI segment of the chromosome of Pseudomonas aeruginosa PA103 which carries the exotoxin A structural gene was expressed from an internal promoter when cloned in a pUC9 derivative and transformed into a nontoxigenic mutant of P. aeruginosa PAO1. The unique terminal EcoRI site was delete...

journal_title：Journal of bacteriology

authors： Tsaur ML,Clowes RC

更新日期：1989-05-01 00:00:00

abstract：:The DNA sequence of part of the gnd (6-phosphogluconate dehydrogenase) gene was determined for eight wild strains of Escherichia coli and for Salmonella typhimurium. Since a region of the trp (tryptophan) operon and the phoA (alkaline phosphatase) gene have been sequenced from the same strains, the gene trees for thes...

journal_title：Journal of bacteriology

authors： Dykhuizen DE,Green L

更新日期：1991-11-01 00:00:00

abstract：:Dehority, B. A. (Ohio Agricultural Experiment Station, Wooster). Degradation and utilization of isolated hemicellulose by pure cultures of cellulolytic rumen bacteria. J. Bacteriol. 89:1515-1520. 1965.-Hemicelluloses isolated from flax, corn hulls, alfalfa, oat hulls, and fescue grass were used as sole energy sources ...

journal_title：Journal of bacteriology

authors： DEHORITY BA

更新日期：1965-06-01 00:00:00

abstract：:Escherichia coli sigma70-dependent promoters have typically been characterized as either -10/-35 promoters, which have good matches to both the canonical -10 and the -35 sequences or as extended -10 promoters (TGn/-10 promoters), which have the TGn motif and an excellent match to the -10 consensus sequence. We report ...

journal_title：Journal of bacteriology

authors： Hook-Barnard I,Johnson XB,Hinton DM

更新日期：2006-12-01 00:00:00

abstract：:The delta subunit of RNA polymerase, RpoE, is widespread in low-G+C Gram-positive bacteria and is thought to play a role in enhancing transcriptional specificity by blocking RNA polymerase binding at weak promoter sites and stimulating RNA synthesis by accelerating core enzyme recycling. Despite the well-studied bioch...

journal_title：Journal of bacteriology

authors： Xue X,Tomasch J,Sztajer H,Wagner-Döbler I

更新日期：2010-10-01 00:00:00

abstract：:LaBrec, Eugene H., Herman Schneider, Thomas J. Magnani, and Samuel B. Formal. Epithelial cell penetration as an essential step in the pathogenesis of bacillary dysentery. J. Bacteriol. 88:1503-1518. 1964.-A parent strain of Shigella flexneri 2a and a colonial mutant derived from it were studied in three animal models....

journal_title：Journal of bacteriology

authors： Labrec EH,Schneider H,Magnani TJ,Formal SB

更新日期：1964-11-01 00:00:00

abstract：:Purified bulk tRNA from Methanococcus vanielii (carbon source, formate) showed variation in the modified nucleoside pattern reported for Escherichia coli as analyzed by both ion-exchange and thin-layer chromatography. Ribothymidine and 7-methylguanosine were absent; 1-methyladenosine, 1-methylguanosine, N2-methylguano...

journal_title：Journal of bacteriology

authors： Best AN

更新日期：1978-01-01 00:00:00

abstract：:A new strategy was developed for rapid cloning of genes with a transposon mutation library. We constructed a transposon designated TnV that was derived from Tn5 and consists of the gene coding for neomycin phosphotransferase II as well as the replication origin of an Escherichia coli plasmid, pSC101, flanked by Tn5 in...

journal_title：Journal of bacteriology

authors： Furuichi T,Inouye M,Inouye S

更新日期：1985-10-01 00:00:00

abstract：:The nucleotide sequence of tnaB of the tryptophanase operon of Escherichia coli is presented. TnaB is a tryptophan-specific permease that is homologous to Mtr, a second tryptophan-specific permease, and to TyrP, a tyrosine-specific permease. Each member of this family appears to contain 11 membrane-spanning domains. ...

journal_title：Journal of bacteriology

authors： Sarsero JP,Wookey PJ,Gollnick P,Yanofsky C,Pittard AJ

更新日期：1991-05-01 00:00:00

abstract：:The Tat system is a recently discovered protein export pathway that serves to translocate folded proteins, often containing redox cofactors, across the bacterial cytoplasmic membrane. Here we report that tat strains are associated with a mutant cell septation phenotype, where chains of up to 10 cells are evident. Muta...

journal_title：Journal of bacteriology

authors： Stanley NR,Findlay K,Berks BC,Palmer T

更新日期：2001-01-01 00:00:00

abstract：:The SecY39(Cs) (cold-sensitive) alteration of Arg357 results in a defect of translocation initiation. As a means to dissect the Sec translocation machinery, we isolated mutations that act as suppressors of the secY39 defect. A specific secE mutation, designated secE105, was thus isolated. This mutation proved to be id...

journal_title：Journal of bacteriology

authors： Mori H,Akiyama Y,Ito K

更新日期：2003-02-01 00:00:00

abstract：:Klite, P. D. (Middle America Research Unit, Balboa Heights, Canal Zone). Intestinal bacterial flora and transit time of three neotropical bat species. J. Bacteriol. 90:375-379. 1965.-Quantitative studies on the intestinal bacterial flora of three neotropical bat species revealed the following average bacterial populat...

journal_title：Journal of bacteriology

authors： KLITE PD

更新日期：1965-08-01 00:00:00

abstract：:The Enterococcus faecalis plasmid pAD1 undergoes conjugative transfer in response to cAD1, a peptide sex pheromone emitted by potential bacterial recipients. Regulation of pAD1 transfer involves a number of plasmid-encoded determinants:iad, which determines a peptide-competitive inhibitor iAD1; signal sensing and tran...

journal_title：Journal of bacteriology

authors： Pontius LT,Clewell DB

更新日期：1992-05-01 00:00:00

abstract：:At various stages during spore formation sporangia were shocked by cold treatment or with toluene, and the germination requirements of the prespores were examined. Up to 5 h after induction of sporulation (t5) germination was spontaneous; i.e., it occurred without any added germinants. After t5, during stages V and VI...

journal_title：Journal of bacteriology

authors： Dion P,Mandelstam J

更新日期：1980-02-01 00:00:00

abstract：:Iron-sulfur flavoproteins (ISF) constitute a widespread family of redox-active proteins in anaerobic prokaryotes. Based on sequence homologies, their overall structure is expected to be similar to that of flavodoxins, but in addition to a flavin mononucleotide cofactor they also contain a cubane-type [4Fe:4S] cluster....

journal_title：Journal of bacteriology

authors： Andrade SL,Cruz F,Drennan CL,Ramakrishnan V,Rees DC,Ferry JG,Einsle O

更新日期：2005-06-01 00:00:00

abstract：:Mycobacterial genomes are endowed with many eukaryote-like nucleotide cyclase genes encoding proteins that can synthesize 3',5'-cyclic AMP (cAMP). However, the roles of cAMP and the need for such redundancy in terms of adenylyl cyclase genes remain unknown. We measured cAMP levels in Mycobacterium smegmatis during gro...

journal_title：Journal of bacteriology

authors： Dass BK,Sharma R,Shenoy AR,Mattoo R,Visweswariah SS

更新日期：2008-06-01 00:00:00

abstract：:The peptidoglycan cell wall determines the shape and structural integrity of a bacterial cell. Class B penicillin-binding proteins (PBPs) carry a transpeptidase activity that cross-links peptidoglycan strands via their peptide side chains, and some of these proteins are directly involved in cell shape determination. N...

journal_title：Journal of bacteriology

authors： Wei Y,Havasy T,McPherson DC,Popham DL

更新日期：2003-08-01 00:00:00

abstract：:Yeast cells growing on mineral medium plus ammonia and glucose contained high levels of nicotinamide adenine dinucleotide phosphate-dependent glutamate dehydrogenase activity, as measured in crude extracts. After suspension of cells in fresh medium lacking glucose, there was a loss of the glutamate dehydrogenase activ...

journal_title：Journal of bacteriology

authors： Mazón MJ

更新日期：1978-02-01 00:00:00

abstract：:Developmental expression of the Myxococcus xanthus gene 4521 requires extracellular A-signal. This signal is generated in response to nutrient limitation and functions in cell density sensing. To identify the upstream limit of the minimum region required in vivo for A-signal-dependent 4521 expression, a 5' deletion an...

journal_title：Journal of bacteriology

authors： Gulati P,Xu D,Kaplan HB

更新日期：1995-08-01 00:00:00

abstract：:Lysates of Escherichia coli Ymel obtained from cultures grown in the absence of tryptophan in minimal medium supplemented with 0.1% casein hydrolysate show an approximate fivefold increase in steady-state specific activity of both anthranilate synthetase and tryptophan synthetase A protein relative to cultures grown i...

journal_title：Journal of bacteriology

authors： Stubbs JD,Stubbs EA

更新日期：1971-12-01 00:00:00

abstract：:A Pediococcus cerevisiae mutant that actively accumulated folate (PteGlu), in contrast to the wild-type, was also found to exhibit changes in the pattern of uptake of 5-methyl-tetrahydrofolate (5-CH3-H4PteGlu) and amethopterin. Most of the 5-CH3-H4PteGlue accumulated through a glucose- and temperature-dependent proces...

journal_title：Journal of bacteriology

authors： Mandelbaum-Shavit F,Grossowicz N

更新日期：1975-08-01 00:00:00

abstract：:Sequence flanking the bfpA locus on the enteroadherent factor plasmid of the enteropathogenic Escherichia coli (EPEC) strain B171-8 (O111:NM) was obtained to identify genes that might be required for bundle-forming pilus (BFP) biosynthesis. Deletion experiments led to the identification of a contiguous cluster of at l...

journal_title：Journal of bacteriology

authors： Sohel I,Puente JL,Ramer SW,Bieber D,Wu CY,Schoolnik GK

更新日期：1996-05-01 00:00:00

abstract：:rpoS, a gene that encodes an alternative sigma factor (also known as katF), is critical for the ability of Yersinia enterocolitica grown at 37 degrees C, but not at 26 degrees C, to survive diverse environmental insults such as high temperature, hydrogen peroxide, osmolarity, and low pH. However, a Y. enterocolitica r...

journal_title：Journal of bacteriology

authors： Badger JL,Miller VL

更新日期：1995-09-01 00:00:00

abstract：:An alcohol dehydrogenase (ADH) from hyperthermophilic archaeon Thermococcus guaymasensis was purified to homogeneity and was found to be a homotetramer with a subunit size of 40 ± 1 kDa. The gene encoding the enzyme was cloned and sequenced; this gene had 1,095 bp, corresponding to 365 amino acids, and showed high seq...

journal_title：Journal of bacteriology

authors： Ying X,Ma K

更新日期：2011-06-01 00:00:00

abstract：UNLABELLED:Campylobacter jejuni is a leading cause of bacterial diarrheal disease and a frequent commensal of the intestinal tract in poultry and other animals. For optimal growth and colonization of hosts, C. jejuni employs two-component regulatory systems (TCSs) to monitor environmental conditions and promote proper ...

journal_title：Journal of bacteriology

authors： Luethy PM,Huynh S,Parker CT,Hendrixson DR

更新日期：2015-05-01 00:00:00

abstract：:A method has been developed for studying the specific activity of the pool of S-adenosylmethionine in yeast. The pool reaches half-maximal specific activity within 30 s after the addition of [methyl-3H]methionine. After addition of an excess of nonradioactive methionine, the specific activity of S-adenosylmethionine i...

journal_title：Journal of bacteriology

authors： Warner JR,Morgan SA,Shulman RW

更新日期：1976-03-01 00:00:00