Kinetics of labeling of the S-adenosylmethionine pool of Saccharomyces cerevisiae.

Abstract:

:A method has been developed for studying the specific activity of the pool of S-adenosylmethionine in yeast. The pool reaches half-maximal specific activity within 30 s after the addition of [methyl-3H]methionine. After addition of an excess of nonradioactive methionine, the specific activity of S-adenosylmethionine is reduced by half within 20 s. During that period there is a substantial expansion of the pool. A logarithmically growing cell in synthetic medium contains about 2 X 10(6) molecules of S-adenosylmethionine, of which only 10% is used for the methylation of ribonucleic acid molecules.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Warner JR,Morgan SA,Shulman RW

doi

10.1128/JB.125.3.887-891.1976

subject

Has Abstract

pub_date

1976-03-01 00:00:00

pages

887-91

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

125

pub_type

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