Characterization of lipoprotein EnvA in Chlamydia psittaci 6BC.

Abstract:

:The primary sequence of the small cysteine-rich protein (EnvA) of Chlamydia psittaci 6BC has been shown to possess a potential lipid modification/signal peptidase II-processing site, and the mature protein was labeled by a [3H]palmitic acid precursor. We further characterized the mature EnvA, showing that it lacks the N-terminal methionine of the primary peptide, is hydrophobic despite a peptide sequence that is predicted to be hydrophilic, and appears to be lipid modified at an N-terminal cysteine in a manner analogous to that of murein lipoproteins of gram-negative bacteria. We also report the fatty acid content of the small cysteine-rich proteins of C. psittaci and Chlamydia trachomatis L2 as determined by combined gas chromatography-mass spectrometry.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Everett KD,Desiderio DM,Hatch TP

doi

10.1128/jb.176.19.6082-6087.1994

subject

Has Abstract

pub_date

1994-10-01 00:00:00

pages

6082-7

issue

19

eissn

0021-9193

issn

1098-5530

journal_volume

176

pub_type

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